FvH4_3g36680

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003720
Physical Location & Seq
Forward (+)
31440483 .. 31444128
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36680.t2

Sequence Viewer

Length: 2505 bp
ATGGATCCAACTTACACTGGACTCCCTGATTTCATGAACGACATTGATTTCGAGCCCATGTTACCCAATTCATCTCAATTCCCAGACATCTCAAATCAGTACCAATTTAACCAGCTCTCTCCAGATCTTGATTTTCTGAGCAATCAGTTTTCAATTCCACCGGAACCGGAATCCGGTAATCTTGTTCCTCCAATTAGTGTTAGCACAGAGGGAGAGTCATTTAATCCTAATGGAGGCTCATTTTCTGGTCCTACTACTGTGAGCCCTGGAGTGGACTCTCCTTCCTCTGATGATATTGACTTCTCTGAAACTGTGTTCAAGTATGTAAACCAGATGCTTCTAGAGGAGAACGTAGAGCAAATGCCTATCATGTTCCATGATCCATTGGGCCTTCGTGTCACCGAGAAATCTTTCTTTGATGTTCTTGGCCAACAGTATCCATTCTCACCAAATCAGCAACAACAACAACCACAACAACAACAGCAAACACTTTATGTTGATCAACCCAACCGGCAACCGCTTTATGTTGACCAAAAAGTTGAGAGCCCTGATGATTATTTTGCCGGAAGCAGTAGTGATTTCAATGCTAGTAGTAGTAGTCCTAGTACTGGCAGTAGCAACTCGGCTGATTATCAGTCGCGTGGTGAATCTGGAGAGCAGAAGCCATCTTTTTCACAAACCTCTCTTTCTAGTGACAGTGTGAACCATTCCACTTCCAATTCCAATTCGCAACCGTCTCTTCCTCTGACAAATAGCCTGTCTACCTTTGGTGATGGGATGTTGGAAGCTTCTGTGAATCAGTTTCTGGCTCAGAATATATTTACAGATAGTGAATCTGTCTTGCAGTTTCAGAGAGGGGTAGAGGAAGCTAGTAAGTTCCTTCCTAAAGTTAATCCATTGGTTATTGATTTGGAAAGCAGTAGTGTGTCTTCAGAAGTGAAAGGACATGCTTCAACTGTTGTCGTCAAGAAGGAAAAGAGTGAGAGGAAGACCTCACCTAGTAAGCGAAGGGGAAGGAGGAACTCACCGAATGGGTCAATGGAAAGGAAGAAGTCACCTAATGGGTCAAAAGGAAAGAAGAATCACGAGCGAGAAGATGTGGATTCTGAAGGGAGGAGTAGCAAGCAGTCTGCAGTTTTTAAGGAGGAGATGGAGGAGGAGGAGGAGGAATTATCTGAGTTGTTTGACAAGGTCTTGCTTTGTACGGACGGGGGTAATCAGTCTTCATGTGGTTCATGTGGCGACAATGTAGATGTGAAGAATGAAGAAAGCAAAAACTTACAGCCAAATGGACAGCCACTGGCATCAAATGGTGAAGGTGGGAAGGCTCGTGCTAAGAAACAAGGCAAGAAGAAGGAAACTGTGGATTTAAGAAATCTCCTGATTCTCTGTGCACAAGCTGTGTCTGTCAATGATTATAGGACCTCTACCGAACTACTAAAGCAAATTAGGCAGCATTCTTCTCCTTTTGGCGATGGATCTCAGAGGTTGGCTCATTTCTTTGCAAATGGCCTGGAGGCACGAATGGCTGGTACTGGCACTGGAACCCAGATTTTTTATACTTCCCTTGCTTCCAAAAAGACATCGGCTGTTGAGATTTTGAAATCTTACCAAGTCAGTCTTGCATCTTGCCCTTTTAAAAGGATGTCAATTTTCTTCAAAAACAAGATGATTTTTAAGATGGCTGAGAAAGCAACTACCCTCCATATTGTGGATTTTGGTATCCTGTATGGTTTTCAGTGGCCAATCCTTATCCACAAGCTTTCAATGAGACCTGGTGGACCTCCCAAGCTACGAATTACAGGGATAGAGGTTCCCCAACCTGGATTTCGCCCAGCAGAGTGGATCGAAGAGACTGGAAGACGCTTGGCAAAATATTGTGAGCGCTTTAAGGTTCCTTTTGAGTTTAATGCGATAGCTTCGCAAAATTGGGAGTCCATCAAAGTAGAGGACCTCAAGGTTGAGAGGAATGAGGTGCTTGCTGTGAATTGTATGTTACGGTTCAAAAACCTACTGGATGAGACAGTTGAAGTGAATTGTCCAAGAGATTCTGTTCTAAAGCTAATCAGGAGTTTGAAACCAGATATTTTTGTTCATACTATTATTAATGGTGCCTACAATGCCCCATTCTTTGTCACTCGATTTCGGGAGGCTCTCTTCCACTTCTCTGCCTTGTATGATGTGTTTGATGTTAATATACCCCGTGACAATCCACAGAGGTTGATGTTTGAAGCAGAGTTCTATGGCCGAGAGGCCATGAATGTGATAGCATGTGAGGGGATAGAGAGGGTTGAGAGGGCCGAGACATACAAGCAGTGGCAAGTACGCTGTCAAAGGGCTGGTTTACAGCTTTTGCCATTGGACCAAGAGTTAGTGAAGGTGTTTAGGGATAAGGTGAAGGAATGGTACCACAAAGATTTCACAATTGATCAAGATAGTAATTGGATGCTTCAAGGATGGAAAGGCCGAATTGTCTATGCTTCTTCTTGTTGGGTGCCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

835

Amino Acids

93.68

Weight (kDa)

5.4

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 457 - 831 4.7e-114 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2406
AccB1I GGYRCC 3 cut(s) 2111, 2406, 2494
AccB7I CCANNNNNTGG 1 cut(s) 1711
AccI GTMKAC 1 cut(s) 761
AccII CGCG 1 cut(s) 640
AciI CCGC 1 cut(s) 518
AclWI GGATC 4 cut(s) 12, 374, 1486, 1853
AcoI YGGCCR 3 cut(s) 427, 1742, 2245
AcuI CTGAAG 2 cut(s) 915, 1128
AfaI GTAC 6 cut(s) 101, 607, 1204, 1534, 2325, 2408
AfeI AGCGCT 1 cut(s) 1886
AfiI CCNNNNNNNGG 6 cut(s) 173, 233, 271, 608, 1711, 1823
AjnI CCWGG 4 cut(s) 265, 1512, 1774, 1822
AjuI GAANNNNNNNTTGG 2 cut(s) 1812, 1844
AluBI AGCT 9 cut(s) 115, 788, 869, 1400, 1762, 1792, 1919, 2062, 2350
AluI AGCT 9 cut(s) 115, 788, 869, 1400, 1762, 1792, 1919, 2062, 2350
Alw21I GWGCWC 1 cut(s) 1396
Alw26I GTCTC 5 cut(s) 741, 1765, 1847, 2015, 2297
Alw44I GTGCAC 1 cut(s) 1392
AlwI GGATC 4 cut(s) 12, 374, 1486, 1853
AlwNI CAGNNNCTG 2 cut(s) 805, 1300
Aor51HI AGCGCT 1 cut(s) 1886
AoxI GGCC 8 cut(s) 388, 427, 1510, 1742, 2245, 2253, 2298, 2464
ApaLI GTGCAC 1 cut(s) 1392
ApeKI GCWGC 1 cut(s) 1453
ArsI GACNNNNNNTTYG 4 cut(s) 32, 64, 743, 775
AseI ATTAAT 1 cut(s) 2106
Asp700I GAANNNNTTC 1 cut(s) 410
Asp718I GGTACC 1 cut(s) 2406
AspLEI GCGC 1 cut(s) 1887
AspS9I GGNCC 7 cut(s) 248, 388, 1422, 1781, 1951, 2298, 2362
AsuHPI GGTGA 9 cut(s) 391, 438, 656, 782, 987, 1017, 1047, 1325, 2407
AvaII GGWCC 5 cut(s) 248, 1422, 1781, 1951, 2362
BaeGI GKGCMC 1 cut(s) 1396
BalI TGGCCA 2 cut(s) 429, 1744
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 3 cut(s) 2111, 2406, 2494
BanII GRGCYC 3 cut(s) 57, 266, 548
BarI GAAGNNNNNNTAC 6 cut(s) 913, 945, 1207, 1239, 2390, 2422
BauI CACGAG 2 cut(s) 1085, 1329
BbsI GAAGAC 4 cut(s) 921, 995, 1215, 1867
Bbv12I GWGCWC 1 cut(s) 1396
BbvI GCAGC 1 cut(s) 1465
BccI CCATC 7 cut(s) 673, 767, 1144, 1469, 1675, 1946, 2451
BciT130I CCWGG 4 cut(s) 267, 1514, 1776, 1824
BciVI GTATCC 2 cut(s) 447, 1733
BclI TGATCA 2 cut(s) 499, 2428
BcoDI GTCTC 5 cut(s) 741, 1765, 1847, 2015, 2297
BfaI CTAG 6 cut(s) 341, 588, 603, 690, 870, 999
BfmI CTRYAG 1 cut(s) 1131
BfoI RGCGCY 1 cut(s) 1888
BfuI GTATCC 2 cut(s) 447, 1733
BglII AGATCT 1 cut(s) 124
BisI GCNGC 1 cut(s) 1454
BlsI GCNGC 1 cut(s) 1455
BmcAI AGTACT 1 cut(s) 607
Bme1390I CCNGG 4 cut(s) 267, 1514, 1776, 1824
Bme18I GGWCC 5 cut(s) 248, 1422, 1781, 1951, 2362
BmgT120I GGNCC 7 cut(s) 248, 388, 1422, 1781, 1951, 2298, 2362
BmiI GGNNCC 8 cut(s) 6, 165, 1546, 1815, 1896, 2113, 2408, 2496
BmrFI CCNGG 4 cut(s) 267, 1514, 1776, 1824
BmsI GCATC 4 cut(s) 324, 1313, 1634, 2436
BpiI GAAGAC 4 cut(s) 921, 995, 1215, 1867
BplI GAGNNNNNCTC 2 cut(s) 1477, 1509
BpmI CTGGAG 4 cut(s) 105, 288, 672, 1535
BpuEI CTTGAG 1 cut(s) 1940
BsaI GGTCTC 1 cut(s) 1765
BsaJI CCNNGG 1 cut(s) 265
BsaWI WCCGGW 3 cut(s) 160, 166, 173
Bsc4I CCNNNNNNNGG 6 cut(s) 173, 233, 271, 608, 1711, 1823
Bse118I RCCGGY 1 cut(s) 510
Bse1I ACTGG 7 cut(s) 22, 613, 1305, 1540, 1546, 1861, 2019
BseBI CCWGG 4 cut(s) 267, 1514, 1776, 1824
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 5 cut(s) 783, 1650, 2023, 2451, 2462
BseLI CCNNNNNNNGG 6 cut(s) 173, 233, 271, 608, 1711, 1823
BseMII CTCAG 5 cut(s) 128, 824, 1167, 1496, 1677
BseNI ACTGG 7 cut(s) 22, 613, 1305, 1540, 1546, 1861, 2019
BseRI GAGGAG 7 cut(s) 359, 1129, 1160, 1169, 1172, 1175, 1178
BseSI GKGCMC 1 cut(s) 1396
BseXI GCAGC 1 cut(s) 1465
BseYI CCCAGC 1 cut(s) 1834
Bsh1236I CGCG 1 cut(s) 640
BshFI GGCC 8 cut(s) 390, 429, 1512, 1744, 2247, 2255, 2300, 2466
BshNI GGYRCC 3 cut(s) 2111, 2406, 2494
BsiHKAI GWGCWC 1 cut(s) 1396
BsiSI CCGG 5 cut(s) 161, 167, 174, 511, 564
BslI CCNNNNNNNGG 6 cut(s) 173, 233, 271, 608, 1711, 1823
BsmAI GTCTC 5 cut(s) 741, 1765, 1847, 2015, 2297
BsmBI CGTCTC 1 cut(s) 741
BsmI GAATGC 1 cut(s) 1456
BsnI GGCC 8 cut(s) 390, 429, 1512, 1744, 2247, 2255, 2300, 2466
Bso31I GGTCTC 1 cut(s) 1765
Bsp1286I GDGCHC 4 cut(s) 57, 266, 548, 1396
Bsp143I GATC 7 cut(s) 4, 124, 379, 499, 1478, 1845, 2428
BspACI CCGC 1 cut(s) 518
BspANI GGCC 8 cut(s) 390, 429, 1512, 1744, 2247, 2255, 2300, 2466
BspCNI CTCAG 5 cut(s) 129, 823, 1168, 1495, 1678
BspFNI CGCG 1 cut(s) 640
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 8 cut(s) 6, 165, 1546, 1815, 1896, 2113, 2408, 2496
BspMAI CTGCAG 1 cut(s) 1135
BspPI GGATC 4 cut(s) 12, 374, 1486, 1853
BspT107I GGYRCC 3 cut(s) 2111, 2406, 2494
BspTNI GGTCTC 1 cut(s) 1765
BsrFI RCCGGY 1 cut(s) 510
BsrI ACTGG 7 cut(s) 22, 613, 1305, 1540, 1546, 1861, 2019
BssAI RCCGGY 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 7 cut(s) 4, 124, 379, 499, 1478, 1845, 2428
BssSI CACGAG 2 cut(s) 1085, 1329
Bst2BI CACGAG 2 cut(s) 1085, 1329
Bst2UI CCWGG 4 cut(s) 267, 1514, 1776, 1824
Bst4CI ACNGT 9 cut(s) 259, 313, 435, 698, 735, 958, 1363, 2001, 2026
Bst6I CTCTTC 3 cut(s) 744, 1845, 2162
BstC8I GCNNGC 3 cut(s) 1124, 1980, 2499
BstDEI CTNAG 6 cut(s) 137, 810, 1176, 1335, 1482, 1686
BstENI CCTNNNNNAGG 1 cut(s) 231
BstF5I GGATG 5 cut(s) 783, 1650, 2023, 2451, 2462
BstFNI CGCG 1 cut(s) 640
BstH2I RGCGCY 1 cut(s) 1888
BstHHI GCGC 1 cut(s) 1887
BstKTI GATC 7 cut(s) 7, 127, 382, 502, 1481, 1848, 2431
BstMAI GTCTC 5 cut(s) 741, 1765, 1847, 2015, 2297
BstMBI GATC 7 cut(s) 4, 124, 379, 499, 1478, 1845, 2428
BstMWI GCNNNNNNNGC 4 cut(s) 1450, 1526, 1691, 2120
BstNI CCWGG 4 cut(s) 267, 1514, 1776, 1824
BstNSI RCATGY 2 cut(s) 950, 2274
BstSCI CCNGG 4 cut(s) 265, 1512, 1774, 1822
BstSFI CTRYAG 1 cut(s) 1131
BstSLI GKGCMC 1 cut(s) 1396
BstUI CGCG 1 cut(s) 640
BstV1I GCAGC 1 cut(s) 1465
BstV2I GAAGAC 4 cut(s) 921, 995, 1215, 1867
BstX2I RGATCY 3 cut(s) 4, 124, 1478
BstXI CCANNNNNNTGG 1 cut(s) 1842
BstYI RGATCY 3 cut(s) 4, 124, 1478
BsuI GTATCC 2 cut(s) 447, 1733
BsuRI GGCC 8 cut(s) 390, 429, 1512, 1744, 2247, 2255, 2300, 2466
BtgZI GCGATG 1 cut(s) 1488
BtsCI GGATG 5 cut(s) 783, 1650, 2023, 2451, 2462
BtsI GCAGTG 1 cut(s) 2321
BtsIMutI CAGTG 6 cut(s) 15, 703, 1298, 1539, 1745, 2321
Cac8I GCNNGC 3 cut(s) 1124, 1980, 2499
CaiI CAGNNNCTG 2 cut(s) 805, 1300
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 1887
Cfr10I RCCGGY 1 cut(s) 510
Cfr13I GGNCC 7 cut(s) 248, 388, 1422, 1781, 1951, 2298, 2362
CseI GACGC 1 cut(s) 1872
CsiI ACCWGGT 1 cut(s) 1774
Csp6I GTAC 6 cut(s) 100, 606, 1203, 1533, 2324, 2407
CspCI CAANNNNNGTGG 2 cut(s) 2202, 2237
CviAII CATG 9 cut(s) 34, 58, 370, 377, 947, 1227, 1236, 2257, 2271
CviQI GTAC 6 cut(s) 100, 606, 1203, 1533, 2324, 2407
DdeI CTNAG 6 cut(s) 137, 810, 1176, 1335, 1482, 1686
DpnI GATC 7 cut(s) 6, 126, 381, 501, 1480, 1847, 2430
DpnII GATC 7 cut(s) 4, 124, 379, 499, 1478, 1845, 2428
DraI TTTAAA 1 cut(s) 1639
EaeI YGGCCR 3 cut(s) 427, 1742, 2245
Eam1104I CTCTTC 3 cut(s) 744, 1845, 2162
EarI CTCTTC 3 cut(s) 744, 1845, 2162
Eco24I GRGCYC 3 cut(s) 57, 266, 548
Eco31I GGTCTC 1 cut(s) 1765
Eco47I GGWCC 5 cut(s) 248, 1422, 1781, 1951, 2362
Eco47III AGCGCT 1 cut(s) 1886
Eco57I CTGAAG 2 cut(s) 915, 1128
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoO109I RGGNCCY 2 cut(s) 1422, 1951
EcoRII CCWGG 4 cut(s) 265, 1512, 1774, 1822
EcoT38I GRGCYC 3 cut(s) 57, 266, 548
Esp3I CGTCTC 1 cut(s) 741
FaeI CATG 9 cut(s) 37, 61, 373, 380, 950, 1230, 1239, 2260, 2274
FalI AAGNNNNNCTT 2 cut(s) 1605, 1637
FatI CATG 9 cut(s) 33, 57, 369, 376, 946, 1226, 1235, 2256, 2270
FbaI TGATCA 2 cut(s) 499, 2428
FblI GTMKAC 1 cut(s) 761
Fnu4HI GCNGC 1 cut(s) 1454
FokI GGATG 5 cut(s) 790, 1657, 2030, 2458, 2469
FriOI GRGCYC 3 cut(s) 57, 266, 548
Fsp4HI GCNGC 1 cut(s) 1454
FspBI CTAG 6 cut(s) 341, 588, 603, 690, 870, 999
GlaI GCGC 1 cut(s) 1886
GluI GCNGC 1 cut(s) 1454
GsaI CCCAGC 1 cut(s) 1838
GsuI CTGGAG 4 cut(s) 105, 288, 672, 1535
HaeII RGCGCY 1 cut(s) 1888
HaeIII GGCC 8 cut(s) 390, 429, 1512, 1744, 2247, 2255, 2300, 2466
HapII CCGG 5 cut(s) 161, 167, 174, 511, 564
HgaI GACGC 1 cut(s) 1872
HhaI GCGC 1 cut(s) 1887
Hin1II CATG 9 cut(s) 37, 61, 373, 380, 950, 1230, 1239, 2260, 2274
Hin6I GCGC 1 cut(s) 1885
HinP1I GCGC 1 cut(s) 1885
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 2 cut(s) 786, 1760
HpaII CCGG 5 cut(s) 161, 167, 174, 511, 564
HphI GGTGA 9 cut(s) 391, 438, 656, 782, 987, 1017, 1047, 1325, 2407
Hpy166II GTNNAC 8 cut(s) 274, 328, 529, 703, 762, 1394, 1781, 2345
Hpy8I GTNNAC 8 cut(s) 274, 328, 529, 703, 762, 1394, 1781, 2345
HpyCH4III ACNGT 9 cut(s) 259, 313, 435, 698, 735, 958, 1363, 2001, 2026
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 6 cut(s) 844, 1133, 1394, 1505, 1625, 2501
HpyF10VI GCNNNNNNNGC 4 cut(s) 1450, 1526, 1691, 2120
HpyF3I CTNAG 6 cut(s) 137, 810, 1176, 1335, 1482, 1686
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 9 cut(s) 37, 61, 373, 380, 950, 1230, 1239, 2260, 2274
HspAI GCGC 1 cut(s) 1885
KpnI GGTACC 1 cut(s) 2410
Ksp22I TGATCA 2 cut(s) 499, 2428
Kzo9I GATC 7 cut(s) 4, 124, 379, 499, 1478, 1845, 2428
Lsp1109I GCAGC 1 cut(s) 1465
LweI GCATC 4 cut(s) 324, 1313, 1634, 2436
MabI ACCWGGT 1 cut(s) 1774
MaeI CTAG 6 cut(s) 341, 588, 603, 690, 870, 999
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 7 cut(s) 60, 397, 692, 1053, 1995, 2134, 2204
MalI GATC 7 cut(s) 6, 126, 381, 501, 1480, 1847, 2430
MboI GATC 7 cut(s) 4, 124, 379, 499, 1478, 1845, 2428
MfeI CAATTG 1 cut(s) 2424
MflI RGATCY 3 cut(s) 4, 124, 1478
MhlI GDGCHC 4 cut(s) 57, 266, 548, 1396
MlsI TGGCCA 2 cut(s) 429, 1744
MluNI TGGCCA 2 cut(s) 429, 1744
MlyI GAGTC 4 cut(s) 15, 224, 269, 1943
MmeI TCCRAC 2 cut(s) 32, 762
Mox20I TGGCCA 2 cut(s) 429, 1744
MroXI GAANNNNTTC 1 cut(s) 410
MscI TGGCCA 2 cut(s) 429, 1744
MslI CAYNNNNRTG 1 cut(s) 2261
Msp20I TGGCCA 2 cut(s) 429, 1744
MspI CCGG 5 cut(s) 161, 167, 174, 511, 564
MspR9I CCNGG 4 cut(s) 267, 1514, 1776, 1824
MunI CAATTG 1 cut(s) 2424
Mva1269I GAATGC 1 cut(s) 1456
MvaI CCWGG 4 cut(s) 267, 1514, 1776, 1824
MvnI CGCG 1 cut(s) 640
MwoI GCNNNNNNNGC 4 cut(s) 1450, 1526, 1691, 2120
NdeII GATC 7 cut(s) 4, 124, 379, 499, 1478, 1845, 2428
NlaIII CATG 9 cut(s) 37, 61, 373, 380, 950, 1230, 1239, 2260, 2274
NlaIV GGNNCC 8 cut(s) 6, 165, 1546, 1815, 1896, 2113, 2408, 2496
NmeAIII GCCGAG 3 cut(s) 602, 2273, 2326
NmuCI GTSAC 5 cut(s) 397, 692, 1053, 2134, 2204
NspI RCATGY 2 cut(s) 950, 2274
PagI TCATGA 1 cut(s) 33
PctI GAATGC 1 cut(s) 1456
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 8 cut(s) 170, 647, 796, 833, 1081, 1103, 1384, 2048
PflFI GACNNNGTC 1 cut(s) 1190
PflMI CCANNNNNTGG 1 cut(s) 1711
PkrI GCNGC 1 cut(s) 1455
PleI GAGTC 4 cut(s) 15, 223, 269, 1942
PpsI GAGTC 4 cut(s) 15, 223, 269, 1942
PpuMI RGGWCCY 2 cut(s) 1422, 1951
PshBI ATTAAT 1 cut(s) 2106
Psp5II RGGWCCY 2 cut(s) 1422, 1951
Psp6I CCWGG 4 cut(s) 265, 1512, 1774, 1822
PspFI CCCAGC 1 cut(s) 1834
PspGI CCWGG 4 cut(s) 265, 1512, 1774, 1822
PspN4I GGNNCC 8 cut(s) 6, 165, 1546, 1815, 1896, 2113, 2408, 2496
PspPI GGNCC 7 cut(s) 248, 388, 1422, 1781, 1951, 2298, 2362
PspPPI RGGWCCY 2 cut(s) 1422, 1951
PstI CTGCAG 1 cut(s) 1135
PstNI CAGNNNCTG 2 cut(s) 805, 1300
PsuI RGATCY 3 cut(s) 4, 124, 1478
PsyI GACNNNGTC 1 cut(s) 1190
RsaI GTAC 6 cut(s) 101, 607, 1204, 1534, 2325, 2408
RsaNI GTAC 6 cut(s) 100, 606, 1203, 1533, 2324, 2407
RseI CAYNNNNRTG 1 cut(s) 2261
SatI GCNGC 1 cut(s) 1454
Sau3AI GATC 7 cut(s) 4, 124, 379, 499, 1478, 1845, 2428
Sau96I GGNCC 7 cut(s) 248, 388, 1422, 1781, 1951, 2298, 2362
ScaI AGTACT 1 cut(s) 607
SchI GAGTC 4 cut(s) 15, 224, 269, 1943
ScrFI CCNGG 4 cut(s) 267, 1514, 1776, 1824
SduI GDGCHC 4 cut(s) 57, 266, 548, 1396
SexAI ACCWGGT 1 cut(s) 1774
SfaNI GCATC 4 cut(s) 324, 1313, 1634, 2436
SfcI CTRYAG 1 cut(s) 1131
SinI GGWCC 5 cut(s) 248, 1422, 1781, 1951, 2362
SmiMI CAYNNNNRTG 1 cut(s) 2261
SmlI CTYRAG 1 cut(s) 1955
SmoI CTYRAG 1 cut(s) 1955
SsiI CCGC 1 cut(s) 518
SspI AATATT 1 cut(s) 1877
SspMI CTAG 6 cut(s) 341, 588, 603, 690, 870, 999
StyD4I CCNGG 4 cut(s) 265, 1512, 1774, 1822
TaaI ACNGT 9 cut(s) 259, 313, 435, 698, 735, 958, 1363, 2001, 2026
TaiI ACGT 1 cut(s) 354
TaqI TCGA 3 cut(s) 51, 1848, 2140
TatI WGTACW 1 cut(s) 605
TfiI GAWTC 8 cut(s) 170, 647, 796, 833, 1081, 1103, 1384, 2048
TscAI CASTG 6 cut(s) 22, 703, 1305, 1546, 1745, 2321
TseFI GTSAC 5 cut(s) 397, 692, 1053, 2134, 2204
TseI GCWGC 1 cut(s) 1453
Tsp45I GTSAC 5 cut(s) 397, 692, 1053, 2134, 2204
TspDTI ATGAA 8 cut(s) 22, 50, 60, 1215, 1224, 1278, 2084, 2273
TspGWI ACGGA 1 cut(s) 1220
TspRI CASTG 6 cut(s) 22, 703, 1305, 1546, 1745, 2321
Tth111I GACNNNGTC 1 cut(s) 1190
Van91I CCANNNNNTGG 1 cut(s) 1711
VneI GTGCAC 1 cut(s) 1392
VpaK11BI GGWCC 5 cut(s) 248, 1422, 1781, 1951, 2362
VspI ATTAAT 1 cut(s) 2106
XagI CCTNNNNNAGG 1 cut(s) 231
XbaI TCTAGA 1 cut(s) 340
XceI RCATGY 2 cut(s) 950, 2274
XmiI GTMKAC 1 cut(s) 761
XmnI GAANNNNTTC 1 cut(s) 410
XspI CTAG 6 cut(s) 341, 588, 603, 690, 870, 999
ZrmI AGTACT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.