MD03G1089700.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003720
Physical Location & Seq
Reverse (-)
7367711 .. 7371218
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1089700.v1.1.491

Sequence Viewer

Length: 2430 bp
ATGGATCATCCAACTTTCAATGGAGTTCCTGATTACATGAATGGTTTTAGTATTGATGACCTAGCCTTCTCACCCAATTCAACTCAATTCCCAAATCTCATAAATGAATATCAATTTAACCAGCTCTCTCCGGATCTTAACTTTATGGATAATCGTTTTTTCACTCCACCCGATTTCGAACAGGGTAATATTGTTCCTAAAGTTAGTGTGACCACATTTGGAGAGTCGTTTGTTCCATCTAATAGTTTGAGTCCACCAGATGAAGGCTCATTTTCACTGCCTAATACTTTGAGCCCTGGAGGAGGAGACACATCCTCCGATGACAGTGATTTCTCCGAATCTGTTTTCAAATACGTAAACCAGATTCTTATGGAAGAGAATATAGAGAAAAAGCCTTGCATGTTCTATGATCCATTGGGTCTTCGTATGACTGAGAAATCGTTCTACGATGTTCTTGGTCAACAGTACCCCTTTTCACCCAATCAGCAACCCCTTTATATCAATCCGAATGTTGAGAGTCCCGATGGTAATATTTCTGGGAATTGTACTGATTGTAGTGGTAGTACTAATTCTGGTACTAGCAATTCCATTGATCCTCAATGGGTTGGCGATCTAGGAGACCAAAAATCTTCTTTTCCACAAACTTCCCTTCCCAATGACTACCCTTTCCAGTTTAATTCAAAATCCAGTTCACAGTTGTCTGTTCCTCTGGAAAATGGCATGACTAGGCTTGGTGATGGGCTGCGTGTTGGTGATGAGCAGCATGTTGATAATGTGCTGCAGGGCTCTTCTATAGAAGAGTTTGTGGCTCAGAATATATTTACAGATAGTGATTCTATCTTTCAGTTTCAGAGAGGGTTAGAGGAAGCTAGTAAATTCCTTCCGAAAAGCACTAAGTTGCTTGTTGATCTGGAAAGCAACACAGTGTCTCCAGAAGTGAAAGCACATGTGCCGATAGTTACTGTCAAGAAAGAAAAGAGTGAGAGGAAGAACTTACTTAATGGGTCAAAGGGAAGAAAGAATCACAAGCGGGAAGATGTTGATCTCGAAGAAGGGAGGAGTAGCAAGCAGTCTGCAGTTTATTTAGAAGGGACTCAAGAGAGTGAATTATCTGAGATGTTTGACAAGGTGTTGCTTTGTCCTGGCGGAAATAATGAATCTCAGTGTGACAATGTTGCTTTTAAGAATGAAGCAAGCAAGACCTTGCAGCCAAATGGACAGGCACAAGGATCTAATGGTAATGGTGGGAAGGCTCGTGGTAAGAAACAAGGAGATAAGAAGGAAGCCGTGGATTTGCGGAATCTCCTGATTTTATGCGCACAAGCCTTGTCAACCAATGATTTTAAAACCACCAGTGAACTGCTAAAGAAGGTTAGGCAGCACTCTTCTCCTAACGGTGATGGATCTCAAAGATTGGCTCACTTCTTTGCAAATGGTCTTGAGGCTCGTATGGCTGGTACTGGCACTGGAACCCAAATGTTTTATACGTCCCTTGCTTCCAAAAGGACACCAGCTGTTGAGATTTTGAAAGCTTACCAAGTTCATCTTTCTGCTTGTCCCTTCAAGAGAATGTCAATTTTCTTCAAAAACAAGATGATTTTGAAGATGGCCGAGAAAGCAACAACCCTTCATATTGTCGATTTTGGTATCCTGTATGGTTTCCAGTGGCCCATCCTCATCCAGCATCTTTCAAAGAGAGCTGGAGGACCTCCGAAGCTACGCATTACAGGGATAGAGGTTCCCCAACCTGGGTTTCGTCCAGCAGAGTGGATTGACGAGACTGGACGTCGCTTGGCAAGATATTGTGAGCGTTTTAATGTGCCTTTTGAGTACAATGCGATAGCTTCACAAAACTGGGAATCCATCCAACTGGAGGACCTCAAGACTGAAAGGAACGAGGTGCTTGCTGTGAATTCTATGTTACGGTTCAAGAACCTACTTGACGAGACAGCTGAAGTGAACTGTCCAAGGGATGCTGTTCTAAAACTAATAAGAAGGATGAAGCCAGATATTTTTGTGCACTCTATTATCAATGGTTCCTACACTGCCCCTTTCTTTGACACTCGATTTCGGGAGGCTCTCTTCCACTACTCTGCCTTGTATGATGCATTTGATATTAATATACCCCGTGACAATGAAGAGAGGTTGATGTTTGAGAGGGAGTTCTATGGCAGGGAAGTTATGAATGTAATAGCATGTGAGGGCGTAGAGAGGGTTGAGAGGCCTGAGACATACAAGCAGTGGCAGGTTCGATGCATGAGGGCTGGGTTGCAGCTGCTGCCAGTGGACCAAGGTCTACTGAAGATTTTCAAGGATAAGGTGAAGGCATGGTACCACAAGGACTTTGAAATTGATCAAGATAGTGATTGGATGCTCCAAGGATGGAAGGGCCGAATTGTGTATGCTTCTTCTTGTTGGGTGCCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

810

Amino Acids

91.17

Weight (kDa)

5.41

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 432 - 806 1.6e-112 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1789
Acc16I TGCGCA 1 cut(s) 1318
Acc36I ACCTGC 1 cut(s) 2236
Acc65I GGTACC 1 cut(s) 2331
AccB1I GGYRCC 2 cut(s) 2331, 2419
AccI GTMKAC 1 cut(s) 2296
AccIII TCCGGA 1 cut(s) 130
AciI CCGC 3 cut(s) 1030, 1146, 1297
AclWI GGATC 6 cut(s) 12, 141, 404, 587, 1237, 1411
AcoI YGGCCR 1 cut(s) 1608
AcsI RAATTY 2 cut(s) 875, 1912
AcuI CTGAAG 2 cut(s) 1974, 2321
AcyI GRCGYC 1 cut(s) 1786
AfaI GTAC 7 cut(s) 467, 547, 565, 577, 1459, 1832, 2333
AfiI CCNNNNNNNGG 5 cut(s) 263, 302, 1748, 1749, 1873
AflIII ACRYGT 1 cut(s) 946
AhdI GACNNNNNGTC 1 cut(s) 1785
AjnI CCWGG 3 cut(s) 295, 1141, 1747
AjuI GAANNNNNNNTTGG 2 cut(s) 1737, 1769
Alw21I GWGCWC 1 cut(s) 2022
Alw26I GTCTC 6 cut(s) 300, 612, 933, 1772, 1940, 2222
Alw44I GTGCAC 1 cut(s) 2018
AlwI GGATC 6 cut(s) 12, 141, 404, 587, 1237, 1411
AlwNI CAGNNNCTG 1 cut(s) 2278
Aor13HI TCCGGA 1 cut(s) 130
AoxI GGCC 4 cut(s) 1608, 1667, 2222, 2389
ApaLI GTGCAC 1 cut(s) 2018
ApeKI GCWGC 8 cut(s) 742, 760, 778, 1207, 1378, 2272, 2275, 2278
ApoI RAATTY 2 cut(s) 875, 1912
AseI ATTAAT 1 cut(s) 2118
Asp700I GAANNNNTTC 2 cut(s) 440, 2306
Asp718I GGTACC 1 cut(s) 2331
AspLEI GCGC 1 cut(s) 1319
AspS9I GGNCC 5 cut(s) 1668, 1706, 1876, 2287, 2389
AsuHPI GGTGA 6 cut(s) 63, 468, 746, 764, 1409, 2332
AsuII TTCGAA 1 cut(s) 177
AvaII GGWCC 3 cut(s) 1706, 1876, 2287
BaeGI GKGCMC 1 cut(s) 2022
BanI GGYRCC 2 cut(s) 2331, 2419
BanII GRGCYC 2 cut(s) 296, 788
BarI GAAGNNNNNNTAC 2 cut(s) 2315, 2347
BauI CACGAG 1 cut(s) 1254
BbsI GAAGAC 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 2022
BbvI GCAGC 8 cut(s) 729, 765, 772, 1219, 1390, 2262, 2265, 2284
BccI CCATC 8 cut(s) 244, 518, 731, 1394, 1600, 1679, 1871, 2376
BceAI ACGGC 1 cut(s) 1271
BcgI CGANNNNNNTGC 2 cut(s) 1886, 1920
BciT130I CCWGG 3 cut(s) 297, 1143, 1749
BciVI GTATCC 1 cut(s) 1658
BclI TGATCA 1 cut(s) 2353
BcoDI GTCTC 6 cut(s) 300, 612, 933, 1772, 1940, 2222
BfaI CTAG 5 cut(s) 62, 579, 614, 726, 870
BfmI CTRYAG 3 cut(s) 779, 792, 1074
BfuAI ACCTGC 1 cut(s) 2236
BfuI GTATCC 1 cut(s) 1658
BisI GCNGC 8 cut(s) 743, 761, 779, 1208, 1379, 2273, 2276, 2279
BlsI GCNGC 8 cut(s) 744, 762, 780, 1209, 1380, 2274, 2277, 2280
BmcAI AGTACT 1 cut(s) 565
Bme1390I CCNGG 3 cut(s) 297, 1143, 1749
Bme18I GGWCC 3 cut(s) 1706, 1876, 2287
BmeRI GACNNNNNGTC 1 cut(s) 1785
BmgT120I GGNCC 5 cut(s) 1668, 1706, 1876, 2287, 2389
BmiI GGNNCC 5 cut(s) 1471, 1740, 2038, 2333, 2421
BmrFI CCNGG 3 cut(s) 297, 1143, 1749
BmrI ACTGGG 1 cut(s) 1864
BmsI GCATC 5 cut(s) 1693, 1963, 2095, 2243, 2361
BmuI ACTGGG 1 cut(s) 1864
BoxI GACNNNNGTC 1 cut(s) 2292
BpiI GAAGAC 1 cut(s) 413
BpmI CTGGAG 4 cut(s) 318, 915, 1722, 1892
Bpu14I TTCGAA 1 cut(s) 177
BpuEI CTTGAG 3 cut(s) 1080, 1460, 1865
BsaAI YACGTR 1 cut(s) 355
BsaBI GATNNNNATC 1 cut(s) 1041
BsaHI GRCGYC 1 cut(s) 1786
BsaI GGTCTC 1 cut(s) 612
BsaJI CCNNGG 6 cut(s) 295, 1287, 1748, 1967, 2290, 2377
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 913, 943
Bsc4I CCNNNNNNNGG 5 cut(s) 263, 302, 1748, 1749, 1873
Bse8I GATNNNNATC 1 cut(s) 1041
BseAI TCCGGA 1 cut(s) 130
BseBI CCWGG 3 cut(s) 297, 1143, 1749
BseDI CCNNGG 6 cut(s) 295, 1287, 1748, 1967, 2290, 2377
BseGI GGATG 9 cut(s) 7, 311, 1671, 1677, 1863, 1978, 2004, 2376, 2387
BseJI GATNNNNATC 1 cut(s) 1041
BseLI CCNNNNNNNGG 5 cut(s) 263, 302, 1748, 1749, 1873
BseMII CTCAG 5 cut(s) 423, 824, 1104, 1175, 2217
BseRI GAGGAG 3 cut(s) 315, 318, 1072
BseSI GKGCMC 1 cut(s) 2022
BseXI GCAGC 8 cut(s) 729, 765, 772, 1219, 1390, 2262, 2265, 2284
BseYI CCCAGC 1 cut(s) 2264
BshFI GGCC 4 cut(s) 1610, 1669, 2224, 2391
BshNI GGYRCC 2 cut(s) 2331, 2419
BsiHKAI GWGCWC 1 cut(s) 2022
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 4 cut(s) 504, 1105, 1474, 1542
BslI CCNNNNNNNGG 5 cut(s) 263, 302, 1748, 1749, 1873
BsmAI GTCTC 6 cut(s) 300, 612, 933, 1772, 1940, 2222
BsmFI GGGAC 4 cut(s) 504, 1105, 1474, 1542
BsnI GGCC 4 cut(s) 1610, 1669, 2224, 2391
Bso31I GGTCTC 1 cut(s) 612
Bsp119I TTCGAA 1 cut(s) 177
Bsp1286I GDGCHC 3 cut(s) 296, 788, 2022
Bsp13I TCCGGA 1 cut(s) 130
BspACI CCGC 3 cut(s) 1030, 1146, 1297
BspANI GGCC 4 cut(s) 1610, 1669, 2224, 2391
BspCNI CTCAG 5 cut(s) 424, 823, 1105, 1174, 2218
BspEI TCCGGA 1 cut(s) 130
BspLI GGNNCC 5 cut(s) 1471, 1740, 2038, 2333, 2421
BspMAI CTGCAG 2 cut(s) 783, 1078
BspMI ACCTGC 1 cut(s) 2236
BspPI GGATC 6 cut(s) 12, 141, 404, 587, 1237, 1411
BspQI GCTCTTC 1 cut(s) 793
BspT104I TTCGAA 1 cut(s) 177
BspT107I GGYRCC 2 cut(s) 2331, 2419
BspTNI GGTCTC 1 cut(s) 612
BssECI CCNNGG 6 cut(s) 295, 1287, 1748, 1967, 2290, 2377
BssNI GRCGYC 1 cut(s) 1786
BssSI CACGAG 1 cut(s) 1254
BssT1I CCWWGG 3 cut(s) 1967, 2290, 2377
Bst2BI CACGAG 1 cut(s) 1254
Bst2UI CCWGG 3 cut(s) 297, 1143, 1749
Bst4CI ACNGT 8 cut(s) 326, 465, 696, 925, 964, 1397, 1926, 1964
Bst6I CTCTTC 6 cut(s) 369, 792, 793, 1390, 2087, 2133
BstACI GRCGYC 1 cut(s) 1786
BstAPI GCANNNNNTGC 1 cut(s) 2278
BstBAI YACGTR 1 cut(s) 355
BstBI TTCGAA 1 cut(s) 177
BstC8I GCNNGC 4 cut(s) 1067, 1195, 1905, 2424
BstDEI CTNAG 7 cut(s) 432, 810, 894, 1113, 1161, 2226, 2427
BstDSI CCRYGG 1 cut(s) 1287
BstENI CCTNNNNNAGG 1 cut(s) 300
BstF5I GGATG 9 cut(s) 7, 311, 1671, 1677, 1863, 1978, 2004, 2376, 2387
BstHHI GCGC 1 cut(s) 1319
BstMAI GTCTC 6 cut(s) 300, 612, 933, 1772, 1940, 2222
BstMWI GCNNNNNNNGC 3 cut(s) 1451, 1616, 2278
BstNI CCWGG 3 cut(s) 297, 1143, 1749
BstNSI RCATGY 4 cut(s) 403, 767, 950, 2199
BstPAI GACNNNNGTC 1 cut(s) 2292
BstSCI CCNGG 3 cut(s) 295, 1141, 1747
BstSFI CTRYAG 3 cut(s) 779, 792, 1074
BstSLI GKGCMC 1 cut(s) 2022
BstSNI TACGTA 1 cut(s) 355
BstV1I GCAGC 8 cut(s) 729, 765, 772, 1219, 1390, 2262, 2265, 2284
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 3 cut(s) 133, 1229, 1403
BstXI CCANNNNNNTGG 2 cut(s) 1767, 1870
BstYI RGATCY 3 cut(s) 133, 1229, 1403
BsuI GTATCC 1 cut(s) 1658
BsuRI GGCC 4 cut(s) 1610, 1669, 2224, 2391
BtgI CCRYGG 1 cut(s) 1287
BtsCI GGATG 9 cut(s) 7, 311, 1671, 1677, 1863, 1978, 2004, 2376, 2387
BtsI GCAGTG 3 cut(s) 275, 2043, 2246
BveI ACCTGC 1 cut(s) 2236
Cac8I GCNNGC 4 cut(s) 1067, 1195, 1905, 2424
CaiI CAGNNNCTG 1 cut(s) 2278
CfoI GCGC 1 cut(s) 1319
Cfr13I GGNCC 5 cut(s) 1668, 1706, 1876, 2287, 2389
Csp6I GTAC 7 cut(s) 466, 546, 564, 576, 1458, 1831, 2332
CviAII CATG 8 cut(s) 37, 400, 721, 764, 947, 2196, 2257, 2328
CviQI GTAC 7 cut(s) 466, 546, 564, 576, 1458, 1831, 2332
DdeI CTNAG 7 cut(s) 432, 810, 894, 1113, 1161, 2226, 2427
DraI TTTAAA 1 cut(s) 1345
DriI GACNNNNNGTC 1 cut(s) 1785
EaeI YGGCCR 1 cut(s) 1608
Eam1104I CTCTTC 6 cut(s) 369, 792, 793, 1390, 2087, 2133
Eam1105I GACNNNNNGTC 1 cut(s) 1785
EarI CTCTTC 6 cut(s) 369, 792, 793, 1390, 2087, 2133
EciI GGCGGA 1 cut(s) 1161
Eco105I TACGTA 1 cut(s) 355
Eco130I CCWWGG 3 cut(s) 1967, 2290, 2377
Eco147I AGGCCT 1 cut(s) 2224
Eco24I GRGCYC 2 cut(s) 296, 788
Eco31I GGTCTC 1 cut(s) 612
Eco47I GGWCC 3 cut(s) 1706, 1876, 2287
Eco57I CTGAAG 2 cut(s) 1974, 2321
EcoNI CCTNNNNNAGG 1 cut(s) 300
EcoO109I RGGNCCY 2 cut(s) 1706, 1876
EcoRI GAATTC 1 cut(s) 1912
EcoRII CCWGG 3 cut(s) 295, 1141, 1747
EcoT14I CCWWGG 3 cut(s) 1967, 2290, 2377
EcoT22I ATGCAT 2 cut(s) 2110, 2258
EcoT38I GRGCYC 2 cut(s) 296, 788
ErhI CCWWGG 3 cut(s) 1967, 2290, 2377
FaeI CATG 8 cut(s) 40, 403, 724, 767, 950, 2199, 2260, 2331
FalI AAGNNNNNCTT 2 cut(s) 1530, 1562
FaqI GGGAC 4 cut(s) 504, 1105, 1474, 1542
FatI CATG 8 cut(s) 36, 399, 720, 763, 946, 2195, 2256, 2327
FauI CCCGC 1 cut(s) 1023
FbaI TGATCA 1 cut(s) 2353
FblI GTMKAC 1 cut(s) 2296
Fnu4HI GCNGC 8 cut(s) 743, 761, 779, 1208, 1379, 2273, 2276, 2279
FokI GGATG 8 cut(s) 298, 1658, 1664, 1850, 1985, 2011, 2383, 2394
FriOI GRGCYC 2 cut(s) 296, 788
Fsp4HI GCNGC 8 cut(s) 743, 761, 779, 1208, 1379, 2273, 2276, 2279
FspAI RTGCGCAY 1 cut(s) 1318
FspBI CTAG 5 cut(s) 62, 579, 614, 726, 870
FspI TGCGCA 1 cut(s) 1318
GlaI GCGC 1 cut(s) 1318
GluI GCNGC 8 cut(s) 743, 761, 779, 1208, 1379, 2273, 2276, 2279
GsaI CCCAGC 1 cut(s) 2268
GsuI CTGGAG 4 cut(s) 318, 915, 1722, 1892
HaeIII GGCC 4 cut(s) 1610, 1669, 2224, 2391
HapII CCGG 1 cut(s) 131
HhaI GCGC 1 cut(s) 1319
Hin1I GRCGYC 1 cut(s) 1786
Hin1II CATG 8 cut(s) 40, 403, 724, 767, 950, 2199, 2260, 2331
Hin6I GCGC 1 cut(s) 1317
HinP1I GCGC 1 cut(s) 1317
HincII GTYRAC 2 cut(s) 461, 1332
HindII GTYRAC 2 cut(s) 461, 1332
HindIII AAGCTT 1 cut(s) 1530
HpaII CCGG 1 cut(s) 131
HphI GGTGA 6 cut(s) 63, 468, 746, 764, 1409, 2332
Hpy188I TCNGA 8 cut(s) 319, 337, 507, 813, 852, 885, 1114, 1713
Hpy99I CGWCG 1 cut(s) 1791
HpyCH4III ACNGT 8 cut(s) 326, 465, 696, 925, 964, 1397, 1926, 1964
HpyCH4IV ACGT 3 cut(s) 354, 1487, 1786
HpyCH4V TGCA 9 cut(s) 399, 781, 1076, 1207, 1430, 2020, 2108, 2256, 2272
HpyF10VI GCNNNNNNNGC 3 cut(s) 1451, 1616, 2278
HpyF3I CTNAG 7 cut(s) 432, 810, 894, 1113, 1161, 2226, 2427
HpySE526I ACGT 3 cut(s) 354, 1487, 1786
Hsp92I GRCGYC 1 cut(s) 1786
Hsp92II CATG 8 cut(s) 40, 403, 724, 767, 950, 2199, 2260, 2331
HspAI GCGC 1 cut(s) 1317
Kpn2I TCCGGA 1 cut(s) 130
KpnI GGTACC 1 cut(s) 2335
Ksp22I TGATCA 1 cut(s) 2353
LguI GCTCTTC 1 cut(s) 793
LmnI GCTCC 1 cut(s) 2379
Lsp1109I GCAGC 8 cut(s) 729, 765, 772, 1219, 1390, 2262, 2265, 2284
LweI GCATC 5 cut(s) 1693, 1963, 2095, 2243, 2361
MaeI CTAG 5 cut(s) 62, 579, 614, 726, 870
MaeII ACGT 3 cut(s) 354, 1487, 1786
MaeIII GTNAC 5 cut(s) 208, 958, 1166, 1920, 2129
MflI RGATCY 3 cut(s) 133, 1229, 1403
MhlI GDGCHC 3 cut(s) 296, 788, 2022
MlyI GAGTC 4 cut(s) 233, 259, 526, 1087
MmeI TCCRAC 2 cut(s) 35, 1891
Mph1103I ATGCAT 2 cut(s) 2110, 2258
MroI TCCGGA 1 cut(s) 130
MroXI GAANNNNTTC 2 cut(s) 440, 2306
MseI TTAA 8 cut(s) 117, 138, 675, 999, 1182, 1344, 1815, 2118
MspA1I CMGCKG 3 cut(s) 1514, 1952, 2275
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 3 cut(s) 297, 1143, 1749
MvaI CCWGG 3 cut(s) 297, 1143, 1749
MwoI GCNNNNNNNGC 3 cut(s) 1451, 1616, 2278
NlaIII CATG 8 cut(s) 40, 403, 724, 767, 950, 2199, 2260, 2331
NlaIV GGNNCC 5 cut(s) 1471, 1740, 2038, 2333, 2421
NmeAIII GCCGAG 1 cut(s) 1636
NmuCI GTSAC 3 cut(s) 208, 1166, 2129
NsbI TGCGCA 1 cut(s) 1318
NsiI ATGCAT 2 cut(s) 2110, 2258
NspI RCATGY 4 cut(s) 403, 767, 950, 2199
NspV TTCGAA 1 cut(s) 177
PceI AGGCCT 1 cut(s) 2224
PciI ACATGT 1 cut(s) 946
PciSI GCTCTTC 1 cut(s) 793
PdmI GAANNNNTTC 2 cut(s) 440, 2306
PfeI GAWTC 7 cut(s) 338, 364, 833, 1021, 1157, 1300, 1859
PkrI GCNGC 8 cut(s) 744, 762, 780, 1209, 1380, 2274, 2277, 2280
PleI GAGTC 4 cut(s) 232, 258, 525, 1087
PpsI GAGTC 4 cut(s) 232, 258, 525, 1087
Ppu21I YACGTR 1 cut(s) 355
PpuMI RGGWCCY 2 cut(s) 1706, 1876
PscI ACATGT 1 cut(s) 946
PshAI GACNNNNGTC 1 cut(s) 2292
PshBI ATTAAT 1 cut(s) 2118
Psp5II RGGWCCY 2 cut(s) 1706, 1876
Psp6I CCWGG 3 cut(s) 295, 1141, 1747
PspFI CCCAGC 1 cut(s) 2264
PspGI CCWGG 3 cut(s) 295, 1141, 1747
PspN4I GGNNCC 5 cut(s) 1471, 1740, 2038, 2333, 2421
PspPI GGNCC 5 cut(s) 1668, 1706, 1876, 2287, 2389
PspPPI RGGWCCY 2 cut(s) 1706, 1876
PstI CTGCAG 2 cut(s) 783, 1078
PstNI CAGNNNCTG 1 cut(s) 2278
PsuI RGATCY 3 cut(s) 133, 1229, 1403
PvuII CAGCTG 3 cut(s) 1514, 1952, 2275
RsaI GTAC 7 cut(s) 467, 547, 565, 577, 1459, 1832, 2333
RsaNI GTAC 7 cut(s) 466, 546, 564, 576, 1458, 1831, 2332
SapI GCTCTTC 1 cut(s) 793
SaqAI TTAA 8 cut(s) 117, 138, 675, 999, 1182, 1344, 1815, 2118
SatI GCNGC 8 cut(s) 743, 761, 779, 1208, 1379, 2273, 2276, 2279
Sau96I GGNCC 5 cut(s) 1668, 1706, 1876, 2287, 2389
ScaI AGTACT 1 cut(s) 565
SchI GAGTC 4 cut(s) 233, 259, 526, 1087
ScrFI CCNGG 3 cut(s) 297, 1143, 1749
SduI GDGCHC 3 cut(s) 296, 788, 2022
SfaNI GCATC 5 cut(s) 1693, 1963, 2095, 2243, 2361
SfcI CTRYAG 3 cut(s) 779, 792, 1074
SfuI TTCGAA 1 cut(s) 177
SinI GGWCC 3 cut(s) 1706, 1876, 2287
SmlI CTYRAG 3 cut(s) 1095, 1439, 1880
SmoI CTYRAG 3 cut(s) 1095, 1439, 1880
SnaBI TACGTA 1 cut(s) 355
SseBI AGGCCT 1 cut(s) 2224
SsiI CCGC 3 cut(s) 1030, 1146, 1297
SspI AATATT 2 cut(s) 190, 532
SspMI CTAG 5 cut(s) 62, 579, 614, 726, 870
StuI AGGCCT 1 cut(s) 2224
StyD4I CCNGG 3 cut(s) 295, 1141, 1747
StyI CCWWGG 3 cut(s) 1967, 2290, 2377
TaaI ACNGT 8 cut(s) 326, 465, 696, 925, 964, 1397, 1926, 1964
TaiI ACGT 3 cut(s) 357, 1490, 1789
TaqI TCGA 5 cut(s) 177, 1047, 1638, 2065, 2251
TatI WGTACW 3 cut(s) 545, 563, 1830
TfiI GAWTC 7 cut(s) 338, 364, 833, 1021, 1157, 1300, 1859
Tru1I TTAA 8 cut(s) 117, 138, 675, 999, 1182, 1344, 1815, 2118
Tru9I TTAA 8 cut(s) 117, 138, 675, 999, 1182, 1344, 1815, 2118
TseFI GTSAC 3 cut(s) 208, 1166, 2129
TseI GCWGC 8 cut(s) 742, 760, 778, 1207, 1378, 2272, 2275, 2278
Tsp45I GTSAC 3 cut(s) 208, 1166, 2129
VneI GTGCAC 1 cut(s) 2018
VpaK11BI GGWCC 3 cut(s) 1706, 1876, 2287
VspI ATTAAT 1 cut(s) 2118
XagI CCTNNNNNAGG 1 cut(s) 300
XapI RAATTY 2 cut(s) 875, 1912
XceI RCATGY 4 cut(s) 403, 767, 950, 2199
XmiI GTMKAC 1 cut(s) 2296
XmnI GAANNNNTTC 2 cut(s) 440, 2306
XspI CTAG 5 cut(s) 62, 579, 614, 726, 870
ZraI GACGTC 1 cut(s) 1787
ZrmI AGTACT 1 cut(s) 565
Zsp2I ATGCAT 2 cut(s) 2110, 2258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.