pycom11g08320

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003720
Physical Location & Seq
Reverse (-)
6447579 .. 6449852
2274 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g08320.1

Sequence Viewer

Length: 2274 bp
ATGGACAATCAATTTTCCCTTCCACCCGATTTCGAACAGGGTAATATTGTTCCTGAAGTTAGTTTGACCACAAGTGGAGAGTCATTTGTTCCATCCAAGAGTTTGAGTCCACCTGATGGAGGTTCATTGTCTCTGCCTACGACTGTGAGCGTCGGAGGAGCAGACAGTTCCTCCGATGACAGTGATTTCTCTGAAGCTATTTTCAAATACGCAAACCAGATTCTTTTGGAAGAGAACATAGAGAAAAAGCCATGCATGTTCTTTGATCCGTTGGGTCTTCGTGTGACTGAGAAATCGTTCTATGATGTTCTTGGTCAACAGTACCCCTTTTCACCCAATCAGCAACCCCTTCATATCAATCCGAATGTTGAGAGTCCTGATGGTAATATTTCTGGGAATTGTAGTGATTGTAGTGGTAGTAATAGGAGTTCTAGTCCTGGTACTAGCAATTCCATTGATCCTCAATGGGTTGGTGATTCGGGAGAGCAAAAACCTTCTTTTCCACAAACTTCCCTTCCCAATGACAGCCCCTTCCAGTTTAATTCACATTCCAGTTCACAGTTGTCTGTTCCTCTGGAAAATCGCATGACTAGTGTCGGTGATGGGCAGCATGTTGGTGATATGCTGCAGGGCTCCTCTGCAGAAGAGTTTGTGGCTCAGAATATATTTACAGACAGTGATTCTATCTTGCAATTCCAGAGAGGGTTAGAGGAAGCTAGTAAATTCCTTCCGAAAAGCACTCAGCTGCTTGTTGATCTGGAAAGCAACACAGTGTCTCCAGAAGTGAAAGCAAATGTGCCGATAGTTACTGTCAAGAAAGAAAAGAGTGAGAGGAAGAAGTCACCTAATGGGTCAAAGGGAAGAAAGAATCGCGAGCGGGAAGATGTTGATCTTGAAGAAGGGAGGAGTAGCAAGCAGTCTGCAGTTTATAAAGAAAAGACTGAAGAGAGTGAATTGTCTGAAATGTTTGACAAGGTGTTGCTGTCTACTGGCGGAAATAATGAATCTCAGTGTGATAGTGTTGCTTTTAAGAATGAAGCAAGCAAGACCTTGCAGCCAAGTGGACAGCCACAAGGATCTAATGGTAATGGAGGGAAGGCTCGTGGTAAGAAACAAGGAAAGAAGAAGGAAACTGTGGATTTGCGGAACCTCCTGATTTTATGTGCACAAGCTGTGTCTACCAATGATTTTAGGACCACTAGTGAACTGTTAAAGCAGGTTAGGCAGCATGCTTCTCCTAACGGTGATGGATGTCAAAGGTTGGCTCACTTCTTTGCGAATGGTCTTGAGGCTCGTATGGCTGGTACTGGCACCGGAACCCAAAAGTTTTATACGTCCCTTGCCAAAAGGTCATCAGCTGTTGATGTTTTGAAATCTTACCAAGTCCATCTTTCAGCTTGCCCTTTCAAGAGAATGTGTATGCTCTTCAAAAACAAGATGATTTTGAAGATGGCCGAGAAAGCAACAACCCTTCATATTGTCGATTTTGGTATCCTGTATGGTTTCCAGTGGCCAATCCTCATCCAGCATCTTTCAAAGAGACATGGTGGACCTCCGAAGCTACGCATTACTGGAATAGAGGTTCCCCAACCCGGGTTTCGTCCAGCAGAGTGGATTGAAGAGACTGGACGTCGCTTGGGAAGATATTGTGAGCGTTTTGGTGTACCTTTTGAGTACAATGCCGTAGCTTCACAAAATTGGGAATCCATCCAACTGGCGGACCTCAAGACTGAAAGGAACGAGGTGCTTGCTGTGAACTGTATGTTACGGTTCAAGAACCTACTTGATGAGACTGTTGAAGAGAACTGTCCAAGGGATGCTGTTCTAAATCTAATCAGAAAGATGAAACCAGATATTTTTGTCCACTCTATTATCAATGGTTCCTACAATGCGCCTTTCTTTGTCACTCGATTCCGGGAGGCTCTCTTCCACTACTCTTCCTTGTATGATGCATTTGATATTAATATACCCCGTGACAATGAAGAGAGGTTGGGGTTTGAGAGTGAGTTCTATGGCAGGGAAGCTATGAATGTAATAGCATGTGAGGGCGTAGAGAGGGTTGAGAGGCCTGAGACATATAAGCAGTGGCAGGTTCGATGCATGAGGGCTGGTTTACAGCCGTTGCAATTGGACCAAGATTTAGTGAAGATTCTTAAGGATAAGGTGAAGGCATGGTACCACAAAGATTTTGTAGTCGATCAAGATAGTGATTGGATGCTTCAAGGATGGAAGGGCCGAATTGTGCATGCTTCTTCTTGTTGGGTGCCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

758

Amino Acids

84.53

Weight (kDa)

5.86

Isoelectric Point (pI)

54.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 381 - 754 1.5e-113 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 930
AatII GACGTC 1 cut(s) 1633
Acc36I ACCTGC 2 cut(s) 1207, 2080
Acc65I GGTACC 1 cut(s) 2175
AccB1I GGYRCC 3 cut(s) 1310, 2175, 2263
AccB7I CCANNNNNTGG 1 cut(s) 116
AccBSI CCGCTC 1 cut(s) 877
AccI GTMKAC 2 cut(s) 986, 1178
AccII CGCG 1 cut(s) 873
AciI CCGC 4 cut(s) 877, 993, 1144, 1718
AclWI GGATC 3 cut(s) 260, 452, 1084
AcoI YGGCCR 2 cut(s) 1452, 1511
AcsI RAATTY 1 cut(s) 722
AcuI CTGAAG 3 cut(s) 75, 213, 963
AcyI GRCGYC 1 cut(s) 1630
AfaI GTAC 6 cut(s) 323, 442, 1306, 1665, 1676, 2177
AfiI CCNNNNNNNGG 5 cut(s) 116, 119, 1592, 1593, 1717
AflII CTTAAG 1 cut(s) 2153
AhdI GACNNNNNGTC 1 cut(s) 1629
AhlI ACTAGT 2 cut(s) 590, 1199
AjnI CCWGG 1 cut(s) 436
AjuI GAANNNNNNNTTGG 2 cut(s) 1581, 1613
AloI GAACNNNNNNTCC 2 cut(s) 1566, 1598
AluBI AGCT 9 cut(s) 197, 716, 745, 1172, 1358, 1397, 1561, 1688, 2024
AluI AGCT 9 cut(s) 197, 716, 745, 1172, 1358, 1397, 1561, 1688, 2024
Alw21I GWGCWC 1 cut(s) 1168
Alw26I GTCTC 6 cut(s) 135, 780, 1534, 1616, 1784, 2066
Alw44I GTGCAC 1 cut(s) 1164
AlwI GGATC 3 cut(s) 260, 452, 1084
Ama87I CYCGRG 1 cut(s) 1592
AoxI GGCC 4 cut(s) 1452, 1511, 2066, 2233
ApaLI GTGCAC 1 cut(s) 1164
ApeKI GCWGC 5 cut(s) 607, 625, 745, 1054, 1225
ApoI RAATTY 1 cut(s) 722
AseI ATTAAT 1 cut(s) 1962
Asp700I GAANNNNTTC 1 cut(s) 296
Asp718I GGTACC 1 cut(s) 2175
AspLEI GCGC 1 cut(s) 1894
AspS9I GGNCC 5 cut(s) 1194, 1550, 1720, 2131, 2233
AsuC2I CCSGG 3 cut(s) 1593, 1594, 1916
AsuHPI GGTGA 7 cut(s) 324, 485, 611, 629, 834, 1256, 2176
AsuII TTCGAA 1 cut(s) 33
AvaI CYCGRG 1 cut(s) 1592
AvaII GGWCC 4 cut(s) 1194, 1550, 1720, 2131
BaeGI GKGCMC 1 cut(s) 1168
BalI TGGCCA 1 cut(s) 1513
BanI GGYRCC 3 cut(s) 1310, 2175, 2263
BanII GRGCYC 1 cut(s) 635
BarI GAAGNNNNNNTAC 2 cut(s) 2159, 2191
BauI CACGAG 1 cut(s) 1101
BbsI GAAGAC 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 1168
BbvI GCAGC 5 cut(s) 612, 619, 732, 1066, 1237
BccI CCATC 9 cut(s) 100, 110, 374, 596, 1241, 1395, 1444, 1715, 2220
BceAI ACGGC 2 cut(s) 1667, 2104
BcgI CGANNNNNNTGC 2 cut(s) 1730, 1764
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 1 cut(s) 1502
BcnI CCSGG 3 cut(s) 1593, 1594, 1916
BcoDI GTCTC 6 cut(s) 135, 780, 1534, 1616, 1784, 2066
BcuI ACTAGT 2 cut(s) 590, 1199
BfaI CTAG 5 cut(s) 432, 444, 591, 717, 1200
BfmI CTRYAG 3 cut(s) 626, 639, 921
BfrI CTTAAG 1 cut(s) 2153
BfuAI ACCTGC 2 cut(s) 1207, 2080
BfuI GTATCC 1 cut(s) 1502
BisI GCNGC 5 cut(s) 608, 626, 746, 1055, 1226
BlsI GCNGC 5 cut(s) 609, 627, 747, 1056, 1227
Bme1390I CCNGG 4 cut(s) 438, 1593, 1594, 1916
Bme18I GGWCC 4 cut(s) 1194, 1550, 1720, 2131
BmeRI GACNNNNNGTC 1 cut(s) 1629
BmeT110I CYCGRG 1 cut(s) 1592
BmgT120I GGNCC 5 cut(s) 1194, 1550, 1720, 2131, 2233
BmiI GGNNCC 8 cut(s) 634, 1148, 1312, 1318, 1584, 1882, 2177, 2265
BmrFI CCNGG 4 cut(s) 438, 1593, 1594, 1916
BmsI GCATC 5 cut(s) 1537, 1807, 1939, 2087, 2205
BoxI GACNNNNGTC 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 269
BpmI CTGGAG 1 cut(s) 762
Bpu14I TTCGAA 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 1307, 1709
BpuMI CCSGG 3 cut(s) 1593, 1594, 1916
BsaBI GATNNNNATC 1 cut(s) 888
BsaHI GRCGYC 1 cut(s) 1630
BsaJI CCNNGG 2 cut(s) 1592, 1811
BsaWI WCCGGW 1 cut(s) 1313
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 418, 448, 760, 790
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 119, 1592, 1593, 1717
Bse1I ACTGG 8 cut(s) 535, 552, 994, 1312, 1507, 1576, 1630, 1719
Bse8I GATNNNNATC 1 cut(s) 888
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 1592, 1811
BseGI GGATG 7 cut(s) 92, 1256, 1521, 1707, 1822, 2220, 2231
BseJI GATNNNNATC 1 cut(s) 888
BseLI CCNNNNNNNGG 5 cut(s) 116, 119, 1592, 1593, 1717
BseMII CTCAG 5 cut(s) 279, 671, 755, 1022, 2061
BseNI ACTGG 8 cut(s) 535, 552, 994, 1312, 1507, 1576, 1630, 1719
BseRI GAGGAG 3 cut(s) 171, 625, 919
BseSI GKGCMC 1 cut(s) 1168
BseXI GCAGC 5 cut(s) 612, 619, 732, 1066, 1237
Bsh1236I CGCG 1 cut(s) 873
BshFI GGCC 4 cut(s) 1454, 1513, 2068, 2235
BshNI GGYRCC 3 cut(s) 1310, 2175, 2263
BsiHKAI GWGCWC 1 cut(s) 1168
BsiHKCI CYCGRG 1 cut(s) 1592
BsiSI CCGG 3 cut(s) 1314, 1593, 1915
BslFI GGGAC 1 cut(s) 1321
BslI CCNNNNNNNGG 5 cut(s) 116, 119, 1592, 1593, 1717
BsmAI GTCTC 6 cut(s) 135, 780, 1534, 1616, 1784, 2066
BsmFI GGGAC 1 cut(s) 1321
BsnI GGCC 4 cut(s) 1454, 1513, 2068, 2235
BsoBI CYCGRG 1 cut(s) 1592
Bsp119I TTCGAA 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 635, 1168
Bsp143I GATC 6 cut(s) 265, 457, 754, 889, 1076, 2197
Bsp68I TCGCGA 1 cut(s) 873
BspACI CCGC 4 cut(s) 877, 993, 1144, 1718
BspANI GGCC 4 cut(s) 1454, 1513, 2068, 2235
BspCNI CTCAG 5 cut(s) 280, 670, 754, 1021, 2062
BspFNI CGCG 1 cut(s) 873
BspLI GGNNCC 8 cut(s) 634, 1148, 1312, 1318, 1584, 1882, 2177, 2265
BspMAI CTGCAG 3 cut(s) 630, 643, 925
BspMI ACCTGC 2 cut(s) 1207, 2080
BspPI GGATC 3 cut(s) 260, 452, 1084
BspQI GCTCTTC 1 cut(s) 1430
BspT104I TTCGAA 1 cut(s) 33
BspT107I GGYRCC 3 cut(s) 1310, 2175, 2263
BspTI CTTAAG 1 cut(s) 2153
BsrBI CCGCTC 1 cut(s) 877
BsrI ACTGG 8 cut(s) 535, 552, 994, 1312, 1507, 1576, 1630, 1719
BssECI CCNNGG 2 cut(s) 1592, 1811
BssMI GATC 6 cut(s) 265, 457, 754, 889, 1076, 2197
BssNI GRCGYC 1 cut(s) 1630
BssSI CACGAG 1 cut(s) 1101
BssT1I CCWWGG 1 cut(s) 1811
Bst2BI CACGAG 1 cut(s) 1101
Bst2UI CCWGG 1 cut(s) 438
Bst6I CTCTTC 9 cut(s) 225, 639, 939, 1430, 1614, 1794, 1931, 1942, 1977
BstACI GRCGYC 1 cut(s) 1630
BstAFI CTTAAG 1 cut(s) 2153
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 8 cut(s) 875, 914, 1042, 1230, 1399, 1749, 2247, 2268
BstDEI CTNAG 5 cut(s) 288, 657, 741, 1008, 2070
BstENI CCTNNNNNAGG 1 cut(s) 117
BstF5I GGATG 7 cut(s) 92, 1256, 1521, 1707, 1822, 2220, 2231
BstFNI CGCG 1 cut(s) 873
BstHHI GCGC 1 cut(s) 1894
BstKTI GATC 6 cut(s) 268, 460, 757, 892, 1079, 2200
BstMAI GTCTC 6 cut(s) 135, 780, 1534, 1616, 1784, 2066
BstMBI GATC 6 cut(s) 265, 457, 754, 889, 1076, 2197
BstMWI GCNNNNNNNGC 3 cut(s) 1222, 1298, 1460
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 5 cut(s) 259, 614, 1232, 2043, 2249
BstPAI GACNNNNGTC 1 cut(s) 593
BstSCI CCNGG 4 cut(s) 436, 1591, 1592, 1914
BstSFI CTRYAG 3 cut(s) 626, 639, 921
BstSLI GKGCMC 1 cut(s) 1168
BstUI CGCG 1 cut(s) 873
BstV1I GCAGC 5 cut(s) 612, 619, 732, 1066, 1237
BstV2I GAAGAC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 1076
BstXI CCANNNNNNTGG 2 cut(s) 1611, 1714
BstYI RGATCY 1 cut(s) 1076
BsuI GTATCC 1 cut(s) 1502
BsuRI GGCC 4 cut(s) 1454, 1513, 2068, 2235
BtsCI GGATG 7 cut(s) 92, 1256, 1521, 1707, 1822, 2220, 2231
BtsI GCAGTG 1 cut(s) 2090
BtsIMutI CAGTG 6 cut(s) 187, 682, 777, 1016, 1514, 2090
BtuMI TCGCGA 1 cut(s) 873
BveI ACCTGC 2 cut(s) 1207, 2080
Cac8I GCNNGC 8 cut(s) 875, 914, 1042, 1230, 1399, 1749, 2247, 2268
CfoI GCGC 1 cut(s) 1894
Cfr13I GGNCC 5 cut(s) 1194, 1550, 1720, 2131, 2233
Cfr9I CCCGGG 1 cut(s) 1592
CseI GACGC 1 cut(s) 139
Csp6I GTAC 6 cut(s) 322, 441, 1305, 1664, 1675, 2176
CviQI GTAC 6 cut(s) 322, 441, 1305, 1664, 1675, 2176
DdeI CTNAG 5 cut(s) 288, 657, 741, 1008, 2070
DpnI GATC 6 cut(s) 267, 459, 756, 891, 1078, 2199
DpnII GATC 6 cut(s) 265, 457, 754, 889, 1076, 2197
DriI GACNNNNNGTC 1 cut(s) 1629
EaeI YGGCCR 2 cut(s) 1452, 1511
Eam1104I CTCTTC 9 cut(s) 225, 639, 939, 1430, 1614, 1794, 1931, 1942, 1977
Eam1105I GACNNNNNGTC 1 cut(s) 1629
EarI CTCTTC 9 cut(s) 225, 639, 939, 1430, 1614, 1794, 1931, 1942, 1977
EciI GGCGGA 2 cut(s) 1008, 1733
Eco130I CCWWGG 1 cut(s) 1811
Eco147I AGGCCT 1 cut(s) 2068
Eco24I GRGCYC 1 cut(s) 635
Eco47I GGWCC 4 cut(s) 1194, 1550, 1720, 2131
Eco57I CTGAAG 3 cut(s) 75, 213, 963
Eco88I CYCGRG 1 cut(s) 1592
EcoNI CCTNNNNNAGG 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 1811
EcoT22I ATGCAT 3 cut(s) 257, 1954, 2102
EcoT38I GRGCYC 1 cut(s) 635
ErhI CCWWGG 1 cut(s) 1811
FalI AAGNNNNNCTT 2 cut(s) 1374, 1406
FaqI GGGAC 1 cut(s) 1321
FauI CCCGC 1 cut(s) 870
FblI GTMKAC 2 cut(s) 986, 1178
Fnu4HI GCNGC 5 cut(s) 608, 626, 746, 1055, 1226
FokI GGATG 7 cut(s) 79, 1263, 1508, 1694, 1829, 2227, 2238
FriOI GRGCYC 1 cut(s) 635
Fsp4HI GCNGC 5 cut(s) 608, 626, 746, 1055, 1226
FspBI CTAG 5 cut(s) 432, 444, 591, 717, 1200
GlaI GCGC 1 cut(s) 1893
GluI GCNGC 5 cut(s) 608, 626, 746, 1055, 1226
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 4 cut(s) 1454, 1513, 2068, 2235
HapII CCGG 3 cut(s) 1314, 1593, 1915
HgaI GACGC 1 cut(s) 139
HhaI GCGC 1 cut(s) 1894
Hin1I GRCGYC 1 cut(s) 1630
Hin6I GCGC 1 cut(s) 1892
HinP1I GCGC 1 cut(s) 1892
HincII GTYRAC 1 cut(s) 317
HindII GTYRAC 1 cut(s) 317
HpaII CCGG 3 cut(s) 1314, 1593, 1915
HphI GGTGA 7 cut(s) 324, 485, 611, 629, 834, 1256, 2176
Hpy188I TCNGA 9 cut(s) 155, 175, 193, 363, 660, 732, 961, 1557, 1838
Hpy99I CGWCG 2 cut(s) 155, 1635
HpyCH4IV ACGT 2 cut(s) 1334, 1630
HpyF10VI GCNNNNNNNGC 3 cut(s) 1222, 1298, 1460
HpyF3I CTNAG 5 cut(s) 288, 657, 741, 1008, 2070
HpySE526I ACGT 2 cut(s) 1334, 1630
Hsp92I GRCGYC 1 cut(s) 1630
HspAI GCGC 1 cut(s) 1892
KpnI GGTACC 1 cut(s) 2179
Kzo9I GATC 6 cut(s) 265, 457, 754, 889, 1076, 2197
LguI GCTCTTC 1 cut(s) 1430
LmnI GCTCC 2 cut(s) 158, 638
Lsp1109I GCAGC 5 cut(s) 612, 619, 732, 1066, 1237
LweI GCATC 5 cut(s) 1537, 1807, 1939, 2087, 2205
MaeI CTAG 5 cut(s) 432, 444, 591, 717, 1200
MaeII ACGT 2 cut(s) 1334, 1630
MaeIII GTNAC 6 cut(s) 283, 805, 840, 1764, 1903, 1973
MalI GATC 6 cut(s) 267, 459, 756, 891, 1078, 2199
MbiI CCGCTC 1 cut(s) 877
MboI GATC 6 cut(s) 265, 457, 754, 889, 1076, 2197
MfeI CAATTG 1 cut(s) 2126
MflI RGATCY 1 cut(s) 1076
MhlI GDGCHC 2 cut(s) 635, 1168
MlsI TGGCCA 1 cut(s) 1513
MluNI TGGCCA 1 cut(s) 1513
MlyI GAGTC 3 cut(s) 89, 115, 382
MmeI TCCRAC 2 cut(s) 133, 1735
Mox20I TGGCCA 1 cut(s) 1513
Mph1103I ATGCAT 3 cut(s) 257, 1954, 2102
MroXI GAANNNNTTC 1 cut(s) 296
MscI TGGCCA 1 cut(s) 1513
MseI TTAA 5 cut(s) 540, 1029, 1211, 1962, 2154
MslI CAYNNNNRTG 1 cut(s) 615
Msp20I TGGCCA 1 cut(s) 1513
MspA1I CMGCKG 2 cut(s) 745, 1358
MspCI CTTAAG 1 cut(s) 2153
MspI CCGG 3 cut(s) 1314, 1593, 1915
MspR9I CCNGG 4 cut(s) 438, 1593, 1594, 1916
MunI CAATTG 1 cut(s) 2126
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 873
MwoI GCNNNNNNNGC 3 cut(s) 1222, 1298, 1460
NciI CCSGG 3 cut(s) 1593, 1594, 1916
NdeII GATC 6 cut(s) 265, 457, 754, 889, 1076, 2197
NlaIV GGNNCC 8 cut(s) 634, 1148, 1312, 1318, 1584, 1882, 2177, 2265
NmeAIII GCCGAG 1 cut(s) 1480
NmuCI GTSAC 4 cut(s) 283, 840, 1903, 1973
NruI TCGCGA 1 cut(s) 873
NsiI ATGCAT 3 cut(s) 257, 1954, 2102
NspI RCATGY 5 cut(s) 259, 614, 1232, 2043, 2249
NspV TTCGAA 1 cut(s) 33
PaeI GCATGC 2 cut(s) 1232, 2249
PceI AGGCCT 1 cut(s) 2068
PciSI GCTCTTC 1 cut(s) 1430
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 8 cut(s) 220, 476, 680, 868, 1004, 1703, 1911, 2149
PflMI CCANNNNNTGG 1 cut(s) 116
PfoI TCCNGGA 1 cut(s) 1914
PkrI GCNGC 5 cut(s) 609, 627, 747, 1056, 1227
PleI GAGTC 3 cut(s) 88, 114, 381
PpsI GAGTC 3 cut(s) 88, 114, 381
PshAI GACNNNNGTC 1 cut(s) 593
PshBI ATTAAT 1 cut(s) 1962
PsiI TTATAA 1 cut(s) 930
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 8 cut(s) 634, 1148, 1312, 1318, 1584, 1882, 2177, 2265
PspPI GGNCC 5 cut(s) 1194, 1550, 1720, 2131, 2233
PsrI GAACNNNNNNTAC 2 cut(s) 412, 444
PstI CTGCAG 3 cut(s) 630, 643, 925
PsuI RGATCY 1 cut(s) 1076
PvuII CAGCTG 2 cut(s) 745, 1358
RruI TCGCGA 1 cut(s) 873
RsaI GTAC 6 cut(s) 323, 442, 1306, 1665, 1676, 2177
RsaNI GTAC 6 cut(s) 322, 441, 1305, 1664, 1675, 2176
RseI CAYNNNNRTG 1 cut(s) 615
SapI GCTCTTC 1 cut(s) 1430
SaqAI TTAA 5 cut(s) 540, 1029, 1211, 1962, 2154
SatI GCNGC 5 cut(s) 608, 626, 746, 1055, 1226
Sau3AI GATC 6 cut(s) 265, 457, 754, 889, 1076, 2197
Sau96I GGNCC 5 cut(s) 1194, 1550, 1720, 2131, 2233
SchI GAGTC 3 cut(s) 89, 115, 382
ScrFI CCNGG 4 cut(s) 438, 1593, 1594, 1916
SduI GDGCHC 2 cut(s) 635, 1168
SfaNI GCATC 5 cut(s) 1537, 1807, 1939, 2087, 2205
SfcI CTRYAG 3 cut(s) 626, 639, 921
SfuI TTCGAA 1 cut(s) 33
SinI GGWCC 4 cut(s) 1194, 1550, 1720, 2131
SmaI CCCGGG 1 cut(s) 1594
SmiMI CAYNNNNRTG 1 cut(s) 615
SmlI CTYRAG 3 cut(s) 1286, 1724, 2153
SmoI CTYRAG 3 cut(s) 1286, 1724, 2153
SpeI ACTAGT 2 cut(s) 590, 1199
SphI GCATGC 2 cut(s) 1232, 2249
SseBI AGGCCT 1 cut(s) 2068
SsiI CCGC 4 cut(s) 877, 993, 1144, 1718
SspI AATATT 2 cut(s) 46, 388
SspMI CTAG 5 cut(s) 432, 444, 591, 717, 1200
StuI AGGCCT 1 cut(s) 2068
StyD4I CCNGG 4 cut(s) 436, 1591, 1592, 1914
StyI CCWWGG 1 cut(s) 1811
TaiI ACGT 2 cut(s) 1337, 1633
TaqI TCGA 5 cut(s) 33, 1482, 1909, 2095, 2196
TatI WGTACW 1 cut(s) 1674
TfiI GAWTC 8 cut(s) 220, 476, 680, 868, 1004, 1703, 1911, 2149
Tru1I TTAA 5 cut(s) 540, 1029, 1211, 1962, 2154
Tru9I TTAA 5 cut(s) 540, 1029, 1211, 1962, 2154
TscAI CASTG 6 cut(s) 187, 682, 777, 1016, 1514, 2090
TseFI GTSAC 4 cut(s) 283, 840, 1903, 1973
TseI GCWGC 5 cut(s) 607, 625, 745, 1054, 1225
Tsp45I GTSAC 4 cut(s) 283, 840, 1903, 1973
TspDTI ATGAA 8 cut(s) 114, 341, 1017, 1050, 1463, 1859, 1995, 2042
TspGWI ACGGA 1 cut(s) 258
TspMI CCCGGG 1 cut(s) 1592
TspRI CASTG 6 cut(s) 187, 682, 777, 1016, 1514, 2090
Van91I CCANNNNNTGG 1 cut(s) 116
Vha464I CTTAAG 1 cut(s) 2153
VneI GTGCAC 1 cut(s) 1164
VpaK11BI GGWCC 4 cut(s) 1194, 1550, 1720, 2131
VspI ATTAAT 1 cut(s) 1962
XagI CCTNNNNNAGG 1 cut(s) 117
XapI RAATTY 1 cut(s) 722
XceI RCATGY 5 cut(s) 259, 614, 1232, 2043, 2249
XmaI CCCGGG 1 cut(s) 1592
XmiI GTMKAC 2 cut(s) 986, 1178
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 5 cut(s) 432, 444, 591, 717, 1200
ZraI GACGTC 1 cut(s) 1631
Zsp2I ATGCAT 3 cut(s) 257, 1954, 2102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.