RLG00000035798

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003720
Physical Location & Seq
Forward (+)
71832204 .. 71835020
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035798

Sequence Viewer

Length: 2544 bp
ATGGATCCACCTTACACTGGACTCCCTGATTTCATGAACGACATTGATTTCGAGTCCATGTTACCCAATTCATCTCAATTCCCAGAAATCCCAAATCAGTACCAATTTAACCAGCTCTCTCCAGACCTTGACTTTTTGAGCAATCAGTTTTCAATTCCACCGGAGCCGGTATCTGGTAATTTTGTTCCTCCAATTAGTGTCAGCACAGAAGGAGAGTCGCTTCTTCCTAACGGAGGCTCATTTTCTCTTCCTACAACTGTGAGCCCGGGAGTAGATTCTAAGTCCTCTGATGATCTTGACTTCTCTGAAACTGTTTTCAAGTATGTAAACCAGATGCTTATGGAAGAGAACATAGAGGAAAAGCCCCTCATGCTGTATGATGATCCATTGGGCCTTCGTGGCACCGAGAAATCGTTCTATGAAGTTCTGGGTCAACAGTATCCCTTCTCACCCAATCAGCAACCACAGCAACCACTTTATGATGATCAACCCAATCAGCAACCGCTTTATGTTGATCGAAAAGATGAGAGCCCTGATAGTTACTTTTCTGGTAACAGTAGTGATTTCAATACTGGTAGTAGTCCTAGTGCTGGCACTAGCAACTCCGTTGATTCTCAATCGCTTGGTGATTATGGAGAGCAGAAGCCATCTTCATCAGAAACCTCTCATTCCAGGGACAGCGTTTACCAGTTTAATTCTCATTCCAATTCCAATTCGCGGTTGTCCGTTCCTCTGACAAATAGCCTGTCTAGTTTTGGTGGTGGGATGTCTGAATCTTCTGTAAATCAGTTTCTGGCTCAGAATATATTTACAGATAGTGAATCTGTCTTGCAGTTTCAGAGAGGGTTAGAGCAAGCAAGTAAGTTCCTTCCCAAAGTTAATCCATTAGTTGTTGATTTGGAAAGCAGCACAGTGTCTCCAGAAGTGAAAGGACATGCTCCAACTGACATCGTAAACGAGGAAAAGAGTGGGAGGAAGAACCGACCTAGTAGGCGAAGGGGAAGGAGGAAATCACCGAATGGGTCAAGGGAAAGAAAGAACTCACCTGATGGGTCAAGGGAAAGGAAGAACTCACCTGATGGTTCAAGGGGGAGGAAGAATCGTGAGCGAGAAGATGTGGATTCTGAAGAAGGGAGGAGTAGCAAGCAGTCTGCAATTTATAAGGGGGAGGAGGATGAATTATCTGAGATGTTTGACAAGGTCTTGCTTTTCGACGGGAGTAATCCGTCTTCATGTGGTTCATGCGGTGACAATGTAGATGTGCAGAATGAAGAAAGCAAAAACTTGCAGCCCAATGGACACCCACAAGCATCAAATGATGAAGGTGGGAAGGCTCGTGTGAAGAAACAAGGCAAGAAGAAGGAAACTGTGGATTTAAGGAATCTTCTGATTCTGTGTGCACAAGCGGTGTCTACCAATGATTTTAGGACCTCCACAGAACTACTAAAGCAAATTAGGCAGCATTCTTCTCCTTTTGGTGATGGATCTCAAAGGTTGGCTCATTTCTTTGCAAATGGCCTCGAGGCACGAATGGCTGGCACTGGCACTGGAACCCAGTTTTATTATATTTCCGTTGCTTCCAAACAGAAGGCTGTTGATAAGTTGAAATGTTACCAAGTTCATCTTTCAGCTTGCCCTTTTAAGAGAATGTCAATGTTCTTTAAAAACAAGATGATTTTCAAGATGGCTGAGAAAGCAACTACCCTCCATATTGTAGATTTTGGTATCCTGTATGGTTTTCAGTGGCCAATCCTTATCCACAAGCTTTCAATGAGATCTGGTGGGCCTCCCAAGCTACGAATTACAGGAATAGAGGTTCCCCAACCTGGATTTCGTCCAGCAGAGTGGATTGAAGAGACCGGACGCCGCTTGGCGAGATATTGCAAGCGCTTTAATGTTCCTTTTGAGTTTAATGCGATAGCGTCGCAGAATTGGGAATCCATCCGAGTAGAGGACCTCAAGGTTGAGAGGAATGAGGTGCTTGCTGTGAATTGTATGTTACGGTTCACGAATCTACTGGATGAGACAGTTGAAGTGAACTGTCCAAGAGATTCTATTCTAAAGCTAATCAGGAGGTTGAAACCAGATATTTTTGTTCATACTATTGTTAATGGTGCCTACAATGCCCCTTTCTTTGTCACTCGATTTCGGGAGGCTCTCTTCCACTTCTCTGCCTTGTATGATGCATTTGATGTTAATATACCCCGTGACAGTCCAGAGAGGTTGGTGTTTGAAACAGAGTTCTATGGCCAGGAGGCCATGAATGTGATAGCATGTGAGGGGATACAGAGGGTTGAGAGGGCTGAGACATACAAGCAATGGCAGGTACGTTGTCAAAGGGCTGGTTTAAAGCTGTTGCCATTGAACCAAGAGGTAGTGAAGGCGTTTAGGGATAAGGTGAAGGCATGGTACCACAAAGATTTCGCAATTGATCAAGATAGTGATTGGATGCTTCAAGCATGGAAGGGCCGAATTGTCTATGCTTCTTCTTGTTGGTTCAGAGGTCAATTGTGGCAGTACTTTCTTCTTGCAGAAGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

848

Amino Acids

95.84

Weight (kDa)

5.71

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 459 - 832 2.5e-113 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AbsI CCTCGAGG 1 cut(s) 1520
Acc36I ACCTGC 1 cut(s) 2314
Acc65I GGTACC 1 cut(s) 2409
AccB1I GGYRCC 3 cut(s) 401, 2114, 2409
AccI GTMKAC 1 cut(s) 1412
AccII CGCG 1 cut(s) 718
AciI CCGC 5 cut(s) 503, 718, 1245, 1406, 1867
AclWI GGATC 3 cut(s) 12, 377, 1492
AcoI YGGCCR 2 cut(s) 1745, 2248
AcuI CTGAAG 1 cut(s) 1146
AcyI GRCGYC 1 cut(s) 1864
AfaI GTAC 4 cut(s) 101, 2328, 2411, 2519
AfeI AGCGCT 1 cut(s) 1889
AfiI CCNNNNNNNGG 7 cut(s) 17, 173, 233, 590, 717, 1826, 1951
AjnI CCWGG 3 cut(s) 671, 1825, 2250
AjuI GAANNNNNNNTTGG 2 cut(s) 1815, 1847
AloI GAACNNNNNNTCC 2 cut(s) 1800, 1832
AluBI AGCT 6 cut(s) 115, 1631, 1765, 1795, 2065, 2353
AluI AGCT 6 cut(s) 115, 1631, 1765, 1795, 2065, 2353
Alw21I GWGCWC 1 cut(s) 1402
Alw26I GTCTC 4 cut(s) 921, 1850, 2018, 2300
Alw44I GTGCAC 1 cut(s) 1398
AlwI GGATC 3 cut(s) 12, 377, 1492
AlwNI CAGNNNCTG 1 cut(s) 793
Ama87I CYCGRG 2 cut(s) 265, 1520
Aor51HI AGCGCT 1 cut(s) 1889
AoxI GGCC 7 cut(s) 391, 1516, 1745, 1784, 2248, 2256, 2467
ApaLI GTGCAC 1 cut(s) 1398
ApeKI GCWGC 3 cut(s) 906, 1288, 1459
ArsI GACNNNNNNTTYG 4 cut(s) 32, 64, 731, 763
Asp700I GAANNNNTTC 1 cut(s) 413
Asp718I GGTACC 1 cut(s) 2409
AspLEI GCGC 1 cut(s) 1890
AspS9I GGNCC 5 cut(s) 391, 1428, 1784, 1954, 2467
AsuC2I CCSGG 2 cut(s) 266, 267
AsuHPI GGTGA 8 cut(s) 441, 638, 1005, 1035, 1065, 1259, 1490, 2410
AvaI CYCGRG 2 cut(s) 265, 1520
AvaII GGWCC 2 cut(s) 1428, 1954
BaeGI GKGCMC 1 cut(s) 1402
BalI TGGCCA 2 cut(s) 1747, 2250
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 3 cut(s) 401, 2114, 2409
BanII GRGCYC 2 cut(s) 266, 533
BarI GAAGNNNNNNTAC 4 cut(s) 1213, 1245, 2393, 2425
BauI CACGAG 1 cut(s) 1335
BbsI GAAGAC 1 cut(s) 1221
Bbv12I GWGCWC 1 cut(s) 1402
BbvI GCAGC 3 cut(s) 918, 1300, 1471
BccI CCATC 6 cut(s) 655, 1043, 1073, 1475, 1678, 1949
BciT130I CCWGG 3 cut(s) 673, 1827, 2252
BciVI GTATCC 3 cut(s) 450, 1736, 2277
BclI TGATCA 2 cut(s) 484, 2431
BcnI CCSGG 2 cut(s) 266, 267
BcoDI GTCTC 4 cut(s) 921, 1850, 2018, 2300
BfaI CTAG 4 cut(s) 585, 597, 750, 987
BfoI RGCGCY 1 cut(s) 1891
BfuAI ACCTGC 1 cut(s) 2314
BfuI GTATCC 3 cut(s) 450, 1736, 2277
BglI GCCNNNNNGGC 1 cut(s) 399
BglII AGATCT 1 cut(s) 1775
BisI GCNGC 4 cut(s) 907, 1289, 1460, 1867
BlsI GCNGC 4 cut(s) 908, 1290, 1461, 1868
BmcAI AGTACT 1 cut(s) 2519
Bme1390I CCNGG 5 cut(s) 266, 267, 673, 1827, 2252
Bme18I GGWCC 2 cut(s) 1428, 1954
BmeT110I CYCGRG 2 cut(s) 265, 1520
BmgT120I GGNCC 5 cut(s) 391, 1428, 1784, 1954, 2467
BmiI GGNNCC 7 cut(s) 6, 165, 403, 1552, 1818, 2116, 2411
BmrFI CCNGG 5 cut(s) 266, 267, 673, 1827, 2252
BmrI ACTGGG 1 cut(s) 1549
BmsI GCATC 4 cut(s) 324, 1319, 2173, 2439
BmuI ACTGGG 1 cut(s) 1549
BpiI GAAGAC 1 cut(s) 1221
BpmI CTGGAG 2 cut(s) 105, 903
BpuEI CTTGAG 1 cut(s) 1943
BpuMI CCSGG 2 cut(s) 266, 267
BsaHI GRCGYC 1 cut(s) 1864
BsaI GGTCTC 1 cut(s) 1850
BsaJI CCNNGG 2 cut(s) 265, 672
BsaWI WCCGGW 2 cut(s) 160, 1859
BsaXI ACNNNNNCTCC 2 cut(s) 901, 931
Bsc4I CCNNNNNNNGG 7 cut(s) 17, 173, 233, 590, 717, 1826, 1951
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 7 cut(s) 22, 577, 688, 1546, 1552, 1555, 2022
Bse3DI GCAATG 1 cut(s) 2324
BseBI CCWGG 3 cut(s) 673, 1827, 2252
BseDI CCNNGG 2 cut(s) 265, 672
BseGI GGATG 5 cut(s) 771, 1180, 1941, 2026, 2454
BseLI CCNNNNNNNGG 7 cut(s) 17, 173, 233, 590, 717, 1826, 1951
BseMI GCAATG 1 cut(s) 2324
BseMII CTCAG 4 cut(s) 812, 1176, 1680, 2295
BseNI ACTGG 7 cut(s) 22, 577, 688, 1546, 1552, 1555, 2022
BseRI GAGGAG 2 cut(s) 1150, 1184
BseSI GKGCMC 1 cut(s) 1402
BseXI GCAGC 3 cut(s) 918, 1300, 1471
BsgI GTGCAG 1 cut(s) 1283
Bsh1236I CGCG 1 cut(s) 718
BshFI GGCC 7 cut(s) 393, 1518, 1747, 1786, 2250, 2258, 2469
BshNI GGYRCC 3 cut(s) 401, 2114, 2409
BsiHKAI GWGCWC 1 cut(s) 1402
BsiHKCI CYCGRG 2 cut(s) 265, 1520
BsiSI CCGG 4 cut(s) 161, 167, 266, 1860
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 7 cut(s) 17, 173, 233, 590, 717, 1826, 1951
BsmAI GTCTC 4 cut(s) 921, 1850, 2018, 2300
BsmFI GGGAC 1 cut(s) 689
BsmI GAATGC 1 cut(s) 1462
BsnI GGCC 7 cut(s) 393, 1518, 1747, 1786, 2250, 2258, 2469
Bso31I GGTCTC 1 cut(s) 1850
BsoBI CYCGRG 2 cut(s) 265, 1520
Bsp1286I GDGCHC 3 cut(s) 266, 533, 1402
Bsp143I GATC 8 cut(s) 4, 292, 382, 484, 514, 1484, 1775, 2431
BspACI CCGC 5 cut(s) 503, 718, 1245, 1406, 1867
BspANI GGCC 7 cut(s) 393, 1518, 1747, 1786, 2250, 2258, 2469
BspCNI CTCAG 4 cut(s) 811, 1177, 1681, 2296
BspFNI CGCG 1 cut(s) 718
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 7 cut(s) 6, 165, 403, 1552, 1818, 2116, 2411
BspMI ACCTGC 1 cut(s) 2314
BspPI GGATC 3 cut(s) 12, 377, 1492
BspT107I GGYRCC 3 cut(s) 401, 2114, 2409
BspTNI GGTCTC 1 cut(s) 1850
BsrDI GCAATG 1 cut(s) 2324
BsrFI RCCGGY 1 cut(s) 166
BsrI ACTGG 7 cut(s) 22, 577, 688, 1546, 1552, 1555, 2022
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 2 cut(s) 265, 672
BssMI GATC 8 cut(s) 4, 292, 382, 484, 514, 1484, 1775, 2431
BssNI GRCGYC 1 cut(s) 1864
BssSI CACGAG 1 cut(s) 1335
Bst2BI CACGAG 1 cut(s) 1335
Bst2UI CCWGG 3 cut(s) 673, 1827, 2252
Bst6I CTCTTC 4 cut(s) 252, 339, 1848, 2165
BstACI GRCGYC 1 cut(s) 1864
BstC8I GCNNGC 7 cut(s) 592, 855, 1145, 1537, 1633, 1886, 1983
BstDEI CTNAG 5 cut(s) 279, 798, 1185, 1689, 2304
BstENI CCTNNNNNAGG 1 cut(s) 231
BstF5I GGATG 5 cut(s) 771, 1180, 1941, 2026, 2454
BstFNI CGCG 1 cut(s) 718
BstH2I RGCGCY 1 cut(s) 1891
BstHHI GCGC 1 cut(s) 1890
BstKTI GATC 8 cut(s) 7, 295, 385, 487, 517, 1487, 1778, 2434
BstMAI GTCTC 4 cut(s) 921, 1850, 2018, 2300
BstMBI GATC 8 cut(s) 4, 292, 382, 484, 514, 1484, 1775, 2431
BstMWI GCNNNNNNNGC 8 cut(s) 370, 399, 466, 1456, 1532, 1694, 1792, 2123
BstNI CCWGG 3 cut(s) 673, 1827, 2252
BstNSI RCATGY 2 cut(s) 938, 2277
BstSCI CCNGG 5 cut(s) 264, 265, 671, 1825, 2250
BstSLI GKGCMC 1 cut(s) 1402
BstUI CGCG 1 cut(s) 718
BstV1I GCAGC 3 cut(s) 918, 1300, 1471
BstV2I GAAGAC 1 cut(s) 1221
BstX2I RGATCY 3 cut(s) 4, 1484, 1775
BstXI CCANNNNNNTGG 1 cut(s) 1845
BstYI RGATCY 3 cut(s) 4, 1484, 1775
BsuI GTATCC 3 cut(s) 450, 1736, 2277
BsuRI GGCC 7 cut(s) 393, 1518, 1747, 1786, 2250, 2258, 2469
BtsCI GGATG 5 cut(s) 771, 1180, 1941, 2026, 2454
BtsIMutI CAGTG 5 cut(s) 15, 918, 1539, 1545, 1748
BveI ACCTGC 1 cut(s) 2314
Cac8I GCNNGC 7 cut(s) 592, 855, 1145, 1537, 1633, 1886, 1983
CaiI CAGNNNCTG 1 cut(s) 793
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 1890
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 5 cut(s) 391, 1428, 1784, 1954, 2467
Cfr9I CCCGGG 1 cut(s) 265
CseI GACGC 2 cut(s) 1872, 1911
Csp6I GTAC 4 cut(s) 100, 2327, 2410, 2518
CviQI GTAC 4 cut(s) 100, 2327, 2410, 2518
DdeI CTNAG 5 cut(s) 279, 798, 1185, 1689, 2304
DpnI GATC 8 cut(s) 6, 294, 384, 486, 516, 1486, 1777, 2433
DpnII GATC 8 cut(s) 4, 292, 382, 484, 514, 1484, 1775, 2431
DraI TTTAAA 2 cut(s) 1663, 2349
EaeI YGGCCR 2 cut(s) 1745, 2248
Eam1104I CTCTTC 4 cut(s) 252, 339, 1848, 2165
EarI CTCTTC 4 cut(s) 252, 339, 1848, 2165
Eco24I GRGCYC 2 cut(s) 266, 533
Eco31I GGTCTC 1 cut(s) 1850
Eco47I GGWCC 2 cut(s) 1428, 1954
Eco47III AGCGCT 1 cut(s) 1889
Eco57I CTGAAG 1 cut(s) 1146
Eco88I CYCGRG 2 cut(s) 265, 1520
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoO109I RGGNCCY 2 cut(s) 1428, 1954
EcoRII CCWGG 3 cut(s) 671, 1825, 2250
EcoT22I ATGCAT 1 cut(s) 2188
EcoT38I GRGCYC 2 cut(s) 266, 533
FalI AAGNNNNNCTT 2 cut(s) 1608, 1640
FaqI GGGAC 1 cut(s) 689
FbaI TGATCA 2 cut(s) 484, 2431
FblI GTMKAC 1 cut(s) 1412
Fnu4HI GCNGC 4 cut(s) 907, 1289, 1460, 1867
FokI GGATG 5 cut(s) 778, 1187, 1928, 2033, 2461
FriOI GRGCYC 2 cut(s) 266, 533
Fsp4HI GCNGC 4 cut(s) 907, 1289, 1460, 1867
FspBI CTAG 4 cut(s) 585, 597, 750, 987
GlaI GCGC 1 cut(s) 1889
GluI GCNGC 4 cut(s) 907, 1289, 1460, 1867
GsuI CTGGAG 2 cut(s) 105, 903
HaeII RGCGCY 1 cut(s) 1891
HaeIII GGCC 7 cut(s) 393, 1518, 1747, 1786, 2250, 2258, 2469
HapII CCGG 4 cut(s) 161, 167, 266, 1860
HgaI GACGC 2 cut(s) 1872, 1911
HhaI GCGC 1 cut(s) 1890
Hin1I GRCGYC 1 cut(s) 1864
Hin6I GCGC 1 cut(s) 1888
HinP1I GCGC 1 cut(s) 1888
HincII GTYRAC 1 cut(s) 434
HindII GTYRAC 1 cut(s) 434
HindIII AAGCTT 1 cut(s) 1763
HpaII CCGG 4 cut(s) 161, 167, 266, 1860
HphI GGTGA 8 cut(s) 441, 638, 1005, 1035, 1065, 1259, 1490, 2410
Hpy166II GTNNAC 8 cut(s) 328, 434, 685, 955, 1400, 1413, 2007, 2038
Hpy8I GTNNAC 8 cut(s) 328, 434, 685, 955, 1400, 1413, 2007, 2038
Hpy99I CGWCG 2 cut(s) 1217, 1927
HpyCH4IV ACGT 1 cut(s) 2329
HpyCH4V TGCA 9 cut(s) 832, 1154, 1264, 1288, 1400, 1511, 1884, 2186, 2531
HpyF10VI GCNNNNNNNGC 8 cut(s) 370, 399, 466, 1456, 1532, 1694, 1792, 2123
HpyF3I CTNAG 5 cut(s) 279, 798, 1185, 1689, 2304
HpySE526I ACGT 1 cut(s) 2329
Hsp92I GRCGYC 1 cut(s) 1864
HspAI GCGC 1 cut(s) 1888
KpnI GGTACC 1 cut(s) 2413
Ksp22I TGATCA 2 cut(s) 484, 2431
Kzo9I GATC 8 cut(s) 4, 292, 382, 484, 514, 1484, 1775, 2431
LmnI GCTCC 2 cut(s) 163, 943
Lsp1109I GCAGC 3 cut(s) 918, 1300, 1471
LweI GCATC 4 cut(s) 324, 1319, 2173, 2439
MaeI CTAG 4 cut(s) 585, 597, 750, 987
MaeII ACGT 1 cut(s) 2329
MaeIII GTNAC 8 cut(s) 60, 539, 551, 1247, 1610, 1998, 2137, 2207
MalI GATC 8 cut(s) 6, 294, 384, 486, 516, 1486, 1777, 2433
MboI GATC 8 cut(s) 4, 292, 382, 484, 514, 1484, 1775, 2431
MfeI CAATTG 2 cut(s) 2427, 2507
MflI RGATCY 3 cut(s) 4, 1484, 1775
MhlI GDGCHC 3 cut(s) 266, 533, 1402
MlsI TGGCCA 2 cut(s) 1747, 2250
MluNI TGGCCA 2 cut(s) 1747, 2250
MlyI GAGTC 3 cut(s) 15, 62, 224
MmeI TCCRAC 1 cut(s) 965
Mox20I TGGCCA 2 cut(s) 1747, 2250
Mph1103I ATGCAT 1 cut(s) 2188
MroXI GAANNNNTTC 1 cut(s) 413
MscI TGGCCA 2 cut(s) 1747, 2250
MslI CAYNNNNRTG 1 cut(s) 2264
Msp20I TGGCCA 2 cut(s) 1747, 2250
MspI CCGG 4 cut(s) 161, 167, 266, 1860
MspR9I CCNGG 5 cut(s) 266, 267, 673, 1827, 2252
MunI CAATTG 2 cut(s) 2427, 2507
Mva1269I GAATGC 1 cut(s) 1462
MvaI CCWGG 3 cut(s) 673, 1827, 2252
MvnI CGCG 1 cut(s) 718
MwoI GCNNNNNNNGC 8 cut(s) 370, 399, 466, 1456, 1532, 1694, 1792, 2123
NciI CCSGG 2 cut(s) 266, 267
NdeII GATC 8 cut(s) 4, 292, 382, 484, 514, 1484, 1775, 2431
NlaIV GGNNCC 7 cut(s) 6, 165, 403, 1552, 1818, 2116, 2411
NmuCI GTSAC 3 cut(s) 1247, 2137, 2207
NsiI ATGCAT 1 cut(s) 2188
NspI RCATGY 2 cut(s) 938, 2277
PaeR7I CTCGAG 1 cut(s) 1520
PagI TCATGA 1 cut(s) 33
PctI GAATGC 1 cut(s) 1462
PdmI GAANNNNTTC 1 cut(s) 413
PflFI GACNNNGTC 1 cut(s) 1199
PkrI GCNGC 4 cut(s) 908, 1290, 1461, 1868
PleI GAGTC 3 cut(s) 15, 61, 223
PpsI GAGTC 3 cut(s) 15, 61, 223
PpuMI RGGWCCY 2 cut(s) 1428, 1954
PsiI TTATAA 1 cut(s) 1161
Psp5II RGGWCCY 2 cut(s) 1428, 1954
Psp6I CCWGG 3 cut(s) 671, 1825, 2250
PspGI CCWGG 3 cut(s) 671, 1825, 2250
PspN4I GGNNCC 7 cut(s) 6, 165, 403, 1552, 1818, 2116, 2411
PspPI GGNCC 5 cut(s) 391, 1428, 1784, 1954, 2467
PspPPI RGGWCCY 2 cut(s) 1428, 1954
PspXI VCTCGAGB 1 cut(s) 1520
PstNI CAGNNNCTG 1 cut(s) 793
PsuI RGATCY 3 cut(s) 4, 1484, 1775
PsyI GACNNNGTC 1 cut(s) 1199
RsaI GTAC 4 cut(s) 101, 2328, 2411, 2519
RsaNI GTAC 4 cut(s) 100, 2327, 2410, 2518
RseI CAYNNNNRTG 1 cut(s) 2264
SatI GCNGC 4 cut(s) 907, 1289, 1460, 1867
Sau3AI GATC 8 cut(s) 4, 292, 382, 484, 514, 1484, 1775, 2431
Sau96I GGNCC 5 cut(s) 391, 1428, 1784, 1954, 2467
ScaI AGTACT 1 cut(s) 2519
SchI GAGTC 3 cut(s) 15, 62, 224
ScrFI CCNGG 5 cut(s) 266, 267, 673, 1827, 2252
SduI GDGCHC 3 cut(s) 266, 533, 1402
SfaNI GCATC 4 cut(s) 324, 1319, 2173, 2439
Sfr274I CTCGAG 1 cut(s) 1520
SinI GGWCC 2 cut(s) 1428, 1954
SlaI CTCGAG 1 cut(s) 1520
SmaI CCCGGG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 2264
SmlI CTYRAG 2 cut(s) 1520, 1958
SmoI CTYRAG 2 cut(s) 1520, 1958
SsiI CCGC 5 cut(s) 503, 718, 1245, 1406, 1867
SspMI CTAG 4 cut(s) 585, 597, 750, 987
StyD4I CCNGG 5 cut(s) 264, 265, 671, 1825, 2250
TaiI ACGT 1 cut(s) 2332
TaqI TCGA 5 cut(s) 51, 517, 1212, 1521, 2143
TatI WGTACW 1 cut(s) 2517
TauI GCSGC 1 cut(s) 1869
TscAI CASTG 5 cut(s) 22, 918, 1546, 1552, 1748
TseFI GTSAC 3 cut(s) 1247, 2137, 2207
TseI GCWGC 3 cut(s) 906, 1288, 1459
Tsp45I GTSAC 3 cut(s) 1247, 2137, 2207
TspGWI ACGGA 5 cut(s) 246, 595, 715, 1215, 1561
TspMI CCCGGG 1 cut(s) 265
TspRI CASTG 5 cut(s) 22, 918, 1546, 1552, 1748
Tth111I GACNNNGTC 1 cut(s) 1199
VneI GTGCAC 1 cut(s) 1398
VpaK11BI GGWCC 2 cut(s) 1428, 1954
XagI CCTNNNNNAGG 1 cut(s) 231
XceI RCATGY 2 cut(s) 938, 2277
XhoI CTCGAG 1 cut(s) 1520
XmaI CCCGGG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 1412
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 4 cut(s) 585, 597, 750, 987
ZrmI AGTACT 1 cut(s) 2519
Zsp2I ATGCAT 1 cut(s) 2188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.