Rh5DG459300

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003720
Physical Location & Seq
Forward (+)
72964338 .. 72968064
3727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG459300.1

Sequence Viewer

Length: 2469 bp
ATGGATCCACCTTACACTGGACTCCCTGATTTCATGAACGACATTGATTTCGAGTCCATGTTACCCAATTCATCTCAATTCCCAGAAATCCCAAATCAGTACCAATTTAACCAGCTCTCTCCAGACCTTGACTCTTTGAGCAATCAGTTTTCAATTCCACCGGAGCCGGTATCCGGTACTTTTGTTCCTCCAATTAGTGTCAGCACAGAAGGAGAGTCACTTCTTCCTAACGGAGGCTCATTTTCTCTTCCTACAACCGTGAGCCCCGGAGTAGATTCTAAGTCCTCTGATGATCTTGAATTCTCTGAAACTGTTTTCAAGTATGTAAACCAGATGCTTATGGAAGAGAACATAGAGGAAAAGCCCCTCATGCTCTATGATGATCCATTGGGCCTTCATGGCACTGAGAAATCGTTCTATGAAGTTCTTGGTCAACAGTATCCCTTCTCACCCAATCAGCAACCACTTTATGATGATCAACCCAATCAGCAACCGCTTTATGTTGATCGAAAAGATGAGAGCCCTGATAGTTACTTTTCTGGTAACAGTAGTGATTTCAATACTGGTAGTAGTCCTAGTGCTGGCACTAGCAACTCCGTTGATTCTCAATCGCTTGGTGATTATGGAGAGCAGAAGCCATCTTTGTCAGAAACCTCTCGTTCAAGGGACAGTGTTTACCAGTTTAATTCTCATTCCAATTCCAATTCGCAGTTGTCCGTTCCTCTGACAAATAGCCTGTCTAGTTTTGGTGGTGGGATGTCTGAATCTTCTGTAAATCAGTTTCTGGCTCAGAATATATTTACAGATAGTGAATCTGTTTTGCAGTTTCAGAGAGGTTTAGAGCAAGCAAGCAAGTTCCTTCCCAAAGTTAATCCATTAGTTATTGATTTGGAAAGCAGCACAGTGTCTCCAGAAGTGAAAGGACATGCTCCAACTGATATCGTAAACAAGGAAAAGAGTGAGAGGAAGAACCGACCTAGTAGGCGAAGGGGAAGGAGGAAATCACCGAATGGGTCAAGGGAAAGGAAGAACTCACCTGATGGGTCAAGGGGGAGGAAGAATCATGAGCGAGAAGATGTGGATTCTGAAGGAGGGAGGAGTAGGAAGCAGTCTGCAATTTATAAGGGGGAGGAGGATGAATTATCTGAGATGTTTGACAAGGTCTTGCTTTTCGACGGGTCTAATCTGTCTTCATGTGGCTCATGCGGTGACAATGTAGATGTGCAGAATGAAGAAAGCAAAAACTTGCAGCTCAATGGACAGCCACAAGCATCAAATGATGAAGGTGGGAAGGCTCGTGCGAAGAAACAAGGCAAGAAGAAGGAAACTGTGGATTTAAGGAATCTTCTGATTCTGTGTGCACAAGCGGTGTCTACCAATGATTTTAGGACCTCCACAGAACTACTAAAGCAAATTAGGCAGCATTCTTCTCCTTTTGGTGATGGATCTCAAAGGTTGGCTCATTTCTTTGCAAATGGCCTTGAGGCACGAATGGCTGGTACTGGCACTGGAAACCAGTTTTATTATATTTCCGTTGCTTCCAAACAGAAGGCTGTTGATAAGTTGAAATGTTACCAAGTTCATCTTTCAGCTTGCCCTTTTAAGAGAATGTCAATGTTCTTCAAAAACAAGATGATTTTGAAGATGGCTGAGAAAGCAACTACCCTCCATATTGTAGATTTTGGTATCCTGTATGGTTTTCAGTGGCCAATCCTTATCCACAAGCTTTCAATGAGATCTGGTGGGCCTCCCAAGCTACGAATTACAGGGATAGAGGTTCCCCAACCTGGATTTCGTCCAGCAGAGTGGATTGAAGAGACTGGACGCCGCTTGGCGAGATATTGCAAGCGCTTTAATGTTCCTTTTGAGTTTAATGCGATAGCGTCGCAGAATTGGGAATCCATCCAAGTAGAGGACCTCAAGGTTGAGAGGAATGAGGTGCTTGCTGTGAATTGTATGTTACGGTTCACGAATCTACTGGATGAGACAGTTGAAGTGAACTGTCCAAGAGATTCTGTTCTAAAGCTAATCAGGAGGTTGAAACCAGATATTTTTGTTCATACTATTGTTAATGGTGCCTACAATGCCCCTTTCTTTGTCACTCGATTTCGGGAGGCTCTCTTCCACTTCTCTGCTTTGTATGATGCATTTGATGTTAATATACCCCGTGACAGTCCAGAGAGGTTGGTGTTTGAAACAGAGTTCTATGGCCAGGAGGCCATGAATGTGATAGCATGTGAGGGGATACAGAGGGTTGAGAGGGCTGAGACATACAAGCAATGGCAGGTACGTTGTCAAAGGGCTGGTTTAAAGCTGTTGCCATTGAACCAAGAGTTAGTGAAGGCGTTTAGGGATAAGGTGAAGGCATGGTACCACAAAGATTTCGCAATTGATCAAGATAGTGATTGGATGCTTCAAGCATGGAAGGGCCGAATTGTCTATGCTTCTTCTTGTTGGGTGCCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

822

Amino Acids

92.67

Weight (kDa)

5.7

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 446 - 819 8e-114 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1122
Acc36I ACCTGC 1 cut(s) 2275
Acc65I GGTACC 1 cut(s) 2370
AccB1I GGYRCC 3 cut(s) 2075, 2370, 2458
AccI GTMKAC 1 cut(s) 1373
AciI CCGC 4 cut(s) 494, 1206, 1367, 1828
AclWI GGATC 3 cut(s) 12, 377, 1453
AcoI YGGCCR 2 cut(s) 1706, 2209
AcsI RAATTY 1 cut(s) 299
AcuI CTGAAG 1 cut(s) 1107
AcyI GRCGYC 1 cut(s) 1825
AfaI GTAC 5 cut(s) 101, 178, 1501, 2289, 2372
AfeI AGCGCT 1 cut(s) 1850
AfiI CCNNNNNNNGG 6 cut(s) 17, 173, 233, 581, 1787, 1912
AjnI CCWGG 2 cut(s) 1786, 2211
AjuI GAANNNNNNNTTGG 2 cut(s) 1776, 1808
AluBI AGCT 7 cut(s) 115, 1252, 1592, 1726, 1756, 2026, 2314
AluI AGCT 7 cut(s) 115, 1252, 1592, 1726, 1756, 2026, 2314
Alw21I GWGCWC 1 cut(s) 1363
Alw26I GTCTC 4 cut(s) 912, 1811, 1979, 2261
Alw44I GTGCAC 1 cut(s) 1359
AlwI GGATC 3 cut(s) 12, 377, 1453
AlwNI CAGNNNCTG 1 cut(s) 784
Aor51HI AGCGCT 1 cut(s) 1850
AoxI GGCC 7 cut(s) 391, 1477, 1706, 1745, 2209, 2217, 2428
ApaLI GTGCAC 1 cut(s) 1359
ApeKI GCWGC 3 cut(s) 897, 1249, 1420
ApoI RAATTY 1 cut(s) 299
ArsI GACNNNNNNTTYG 4 cut(s) 32, 64, 722, 754
Asp700I GAANNNNTTC 1 cut(s) 413
Asp718I GGTACC 1 cut(s) 2370
AspLEI GCGC 1 cut(s) 1851
AspS9I GGNCC 5 cut(s) 391, 1389, 1745, 1915, 2428
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 7 cut(s) 441, 629, 996, 1026, 1220, 1451, 2371
AvaII GGWCC 2 cut(s) 1389, 1915
BaeGI GKGCMC 1 cut(s) 1363
BaeI ACNNNNGTAYC 2 cut(s) 168, 201
BalI TGGCCA 2 cut(s) 1708, 2211
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 3 cut(s) 2075, 2370, 2458
BanII GRGCYC 2 cut(s) 266, 524
BarI GAAGNNNNNNTAC 2 cut(s) 2354, 2386
BauI CACGAG 1 cut(s) 1296
BbsI GAAGAC 1 cut(s) 1182
Bbv12I GWGCWC 1 cut(s) 1363
BbvI GCAGC 3 cut(s) 909, 1261, 1432
BccI CCATC 5 cut(s) 646, 1034, 1436, 1639, 1910
BciT130I CCWGG 2 cut(s) 1788, 2213
BciVI GTATCC 4 cut(s) 181, 450, 1697, 2238
BclI TGATCA 2 cut(s) 475, 2392
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 4 cut(s) 912, 1811, 1979, 2261
BfaI CTAG 4 cut(s) 576, 588, 741, 978
BfoI RGCGCY 1 cut(s) 1852
BfuAI ACCTGC 1 cut(s) 2275
BfuI GTATCC 4 cut(s) 181, 450, 1697, 2238
BglI GCCNNNNNGGC 1 cut(s) 399
BglII AGATCT 1 cut(s) 1736
BisI GCNGC 4 cut(s) 898, 1250, 1421, 1828
BlsI GCNGC 4 cut(s) 899, 1251, 1422, 1829
Bme1390I CCNGG 3 cut(s) 267, 1788, 2213
Bme18I GGWCC 2 cut(s) 1389, 1915
BmgT120I GGNCC 5 cut(s) 391, 1389, 1745, 1915, 2428
BmiI GGNNCC 6 cut(s) 6, 165, 1779, 2077, 2372, 2460
BmrFI CCNGG 3 cut(s) 267, 1788, 2213
BmsI GCATC 4 cut(s) 324, 1280, 2134, 2400
BpiI GAAGAC 1 cut(s) 1182
BpmI CTGGAG 2 cut(s) 105, 894
BpuEI CTTGAG 2 cut(s) 1502, 1904
BpuMI CCSGG 1 cut(s) 267
BsaHI GRCGYC 1 cut(s) 1825
BsaJI CCNNGG 1 cut(s) 265
BsaWI WCCGGW 2 cut(s) 160, 173
BsaXI ACNNNNNCTCC 2 cut(s) 892, 922
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 173, 233, 581, 1787, 1912
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 8 cut(s) 22, 568, 679, 1507, 1513, 1516, 1825, 1983
Bse3DI GCAATG 1 cut(s) 2285
BseBI CCWGG 2 cut(s) 1788, 2213
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 5 cut(s) 762, 1141, 1902, 1987, 2415
BseLI CCNNNNNNNGG 6 cut(s) 17, 173, 233, 581, 1787, 1912
BseMI GCAATG 1 cut(s) 2285
BseMII CTCAG 5 cut(s) 396, 803, 1137, 1641, 2256
BseNI ACTGG 8 cut(s) 22, 568, 679, 1507, 1513, 1516, 1825, 1983
BseRI GAGGAG 2 cut(s) 1111, 1145
BseSI GKGCMC 1 cut(s) 1363
BseXI GCAGC 3 cut(s) 909, 1261, 1432
BsgI GTGCAG 1 cut(s) 1244
BshFI GGCC 7 cut(s) 393, 1479, 1708, 1747, 2211, 2219, 2430
BshNI GGYRCC 3 cut(s) 2075, 2370, 2458
BsiHKAI GWGCWC 1 cut(s) 1363
BsiSI CCGG 4 cut(s) 161, 167, 174, 267
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 6 cut(s) 17, 173, 233, 581, 1787, 1912
BsmAI GTCTC 4 cut(s) 912, 1811, 1979, 2261
BsmFI GGGAC 1 cut(s) 680
BsmI GAATGC 1 cut(s) 1423
BsnI GGCC 7 cut(s) 393, 1479, 1708, 1747, 2211, 2219, 2430
Bsp1286I GDGCHC 3 cut(s) 266, 524, 1363
Bsp143I GATC 8 cut(s) 4, 292, 382, 475, 505, 1445, 1736, 2392
BspACI CCGC 4 cut(s) 494, 1206, 1367, 1828
BspANI GGCC 7 cut(s) 393, 1479, 1708, 1747, 2211, 2219, 2430
BspCNI CTCAG 5 cut(s) 397, 802, 1138, 1642, 2257
BspHI TCATGA 2 cut(s) 33, 1063
BspLI GGNNCC 6 cut(s) 6, 165, 1779, 2077, 2372, 2460
BspMI ACCTGC 1 cut(s) 2275
BspPI GGATC 3 cut(s) 12, 377, 1453
BspT107I GGYRCC 3 cut(s) 2075, 2370, 2458
BsrDI GCAATG 1 cut(s) 2285
BsrFI RCCGGY 1 cut(s) 166
BsrI ACTGG 8 cut(s) 22, 568, 679, 1507, 1513, 1516, 1825, 1983
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 8 cut(s) 4, 292, 382, 475, 505, 1445, 1736, 2392
BssNI GRCGYC 1 cut(s) 1825
BssSI CACGAG 1 cut(s) 1296
Bst2BI CACGAG 1 cut(s) 1296
Bst2UI CCWGG 2 cut(s) 1788, 2213
Bst6I CTCTTC 4 cut(s) 252, 339, 1809, 2126
BstACI GRCGYC 1 cut(s) 1825
BstC8I GCNNGC 7 cut(s) 583, 846, 850, 1594, 1847, 1944, 2463
BstDEI CTNAG 6 cut(s) 279, 405, 789, 1146, 1650, 2265
BstENI CCTNNNNNAGG 1 cut(s) 231
BstF5I GGATG 5 cut(s) 762, 1141, 1902, 1987, 2415
BstH2I RGCGCY 1 cut(s) 1852
BstHHI GCGC 1 cut(s) 1851
BstKTI GATC 8 cut(s) 7, 295, 385, 478, 508, 1448, 1739, 2395
BstMAI GTCTC 4 cut(s) 912, 1811, 1979, 2261
BstMBI GATC 8 cut(s) 4, 292, 382, 475, 505, 1445, 1736, 2392
BstMWI GCNNNNNNNGC 7 cut(s) 370, 399, 1417, 1493, 1655, 1753, 2084
BstNI CCWGG 2 cut(s) 1788, 2213
BstNSI RCATGY 2 cut(s) 929, 2238
BstSCI CCNGG 3 cut(s) 265, 1786, 2211
BstSLI GKGCMC 1 cut(s) 1363
BstV1I GCAGC 3 cut(s) 909, 1261, 1432
BstV2I GAAGAC 1 cut(s) 1182
BstX2I RGATCY 3 cut(s) 4, 1445, 1736
BstXI CCANNNNNNTGG 1 cut(s) 1806
BstYI RGATCY 3 cut(s) 4, 1445, 1736
BsuI GTATCC 4 cut(s) 181, 450, 1697, 2238
BsuRI GGCC 7 cut(s) 393, 1479, 1708, 1747, 2211, 2219, 2430
BtsCI GGATG 5 cut(s) 762, 1141, 1902, 1987, 2415
BtsIMutI CAGTG 6 cut(s) 15, 402, 676, 909, 1506, 1709
BveI ACCTGC 1 cut(s) 2275
Cac8I GCNNGC 7 cut(s) 583, 846, 850, 1594, 1847, 1944, 2463
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 2 cut(s) 33, 1063
CfoI GCGC 1 cut(s) 1851
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 5 cut(s) 391, 1389, 1745, 1915, 2428
CseI GACGC 2 cut(s) 1833, 1872
Csp6I GTAC 5 cut(s) 100, 177, 1500, 2288, 2371
CviQI GTAC 5 cut(s) 100, 177, 1500, 2288, 2371
DdeI CTNAG 6 cut(s) 279, 405, 789, 1146, 1650, 2265
DpnI GATC 8 cut(s) 6, 294, 384, 477, 507, 1447, 1738, 2394
DpnII GATC 8 cut(s) 4, 292, 382, 475, 505, 1445, 1736, 2392
DraI TTTAAA 1 cut(s) 2310
EaeI YGGCCR 2 cut(s) 1706, 2209
Eam1104I CTCTTC 4 cut(s) 252, 339, 1809, 2126
EarI CTCTTC 4 cut(s) 252, 339, 1809, 2126
Eco24I GRGCYC 2 cut(s) 266, 524
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 2 cut(s) 1389, 1915
Eco47III AGCGCT 1 cut(s) 1850
Eco57I CTGAAG 1 cut(s) 1107
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoO109I RGGNCCY 2 cut(s) 1389, 1915
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 1786, 2211
EcoRV GATATC 1 cut(s) 940
EcoT22I ATGCAT 1 cut(s) 2149
EcoT38I GRGCYC 2 cut(s) 266, 524
FalI AAGNNNNNCTT 2 cut(s) 1569, 1601
FaqI GGGAC 1 cut(s) 680
FbaI TGATCA 2 cut(s) 475, 2392
FblI GTMKAC 1 cut(s) 1373
Fnu4HI GCNGC 4 cut(s) 898, 1250, 1421, 1828
FokI GGATG 5 cut(s) 769, 1148, 1889, 1994, 2422
FriOI GRGCYC 2 cut(s) 266, 524
Fsp4HI GCNGC 4 cut(s) 898, 1250, 1421, 1828
FspBI CTAG 4 cut(s) 576, 588, 741, 978
GlaI GCGC 1 cut(s) 1850
GluI GCNGC 4 cut(s) 898, 1250, 1421, 1828
GsuI CTGGAG 2 cut(s) 105, 894
HaeII RGCGCY 1 cut(s) 1852
HaeIII GGCC 7 cut(s) 393, 1479, 1708, 1747, 2211, 2219, 2430
HapII CCGG 4 cut(s) 161, 167, 174, 267
HgaI GACGC 2 cut(s) 1833, 1872
HhaI GCGC 1 cut(s) 1851
Hin1I GRCGYC 1 cut(s) 1825
Hin6I GCGC 1 cut(s) 1849
HinP1I GCGC 1 cut(s) 1849
HincII GTYRAC 1 cut(s) 434
HindII GTYRAC 1 cut(s) 434
HindIII AAGCTT 1 cut(s) 1724
HpaII CCGG 4 cut(s) 161, 167, 174, 267
HphI GGTGA 7 cut(s) 441, 629, 996, 1026, 1220, 1451, 2371
Hpy166II GTNNAC 8 cut(s) 328, 434, 676, 946, 1361, 1374, 1968, 1999
Hpy8I GTNNAC 8 cut(s) 328, 434, 676, 946, 1361, 1374, 1968, 1999
Hpy99I CGWCG 2 cut(s) 1178, 1888
HpyCH4IV ACGT 1 cut(s) 2290
HpyCH4V TGCA 9 cut(s) 823, 1115, 1225, 1249, 1361, 1472, 1845, 2147, 2465
HpyF10VI GCNNNNNNNGC 7 cut(s) 370, 399, 1417, 1493, 1655, 1753, 2084
HpyF3I CTNAG 6 cut(s) 279, 405, 789, 1146, 1650, 2265
HpySE526I ACGT 1 cut(s) 2290
Hsp92I GRCGYC 1 cut(s) 1825
HspAI GCGC 1 cut(s) 1849
KpnI GGTACC 1 cut(s) 2374
Ksp22I TGATCA 2 cut(s) 475, 2392
Kzo9I GATC 8 cut(s) 4, 292, 382, 475, 505, 1445, 1736, 2392
LmnI GCTCC 2 cut(s) 163, 934
Lsp1109I GCAGC 3 cut(s) 909, 1261, 1432
LweI GCATC 4 cut(s) 324, 1280, 2134, 2400
MaeI CTAG 4 cut(s) 576, 588, 741, 978
MaeII ACGT 1 cut(s) 2290
MaeIII GTNAC 9 cut(s) 60, 216, 530, 542, 1208, 1571, 1959, 2098, 2168
MalI GATC 8 cut(s) 6, 294, 384, 477, 507, 1447, 1738, 2394
MboI GATC 8 cut(s) 4, 292, 382, 475, 505, 1445, 1736, 2392
MfeI CAATTG 1 cut(s) 2388
MflI RGATCY 3 cut(s) 4, 1445, 1736
MhlI GDGCHC 3 cut(s) 266, 524, 1363
MlsI TGGCCA 2 cut(s) 1708, 2211
MluNI TGGCCA 2 cut(s) 1708, 2211
MlyI GAGTC 4 cut(s) 15, 62, 125, 224
MmeI TCCRAC 1 cut(s) 956
Mox20I TGGCCA 2 cut(s) 1708, 2211
Mph1103I ATGCAT 1 cut(s) 2149
MroXI GAANNNNTTC 1 cut(s) 413
MscI TGGCCA 2 cut(s) 1708, 2211
MslI CAYNNNNRTG 1 cut(s) 2225
Msp20I TGGCCA 2 cut(s) 1708, 2211
MspI CCGG 4 cut(s) 161, 167, 174, 267
MspR9I CCNGG 3 cut(s) 267, 1788, 2213
MunI CAATTG 1 cut(s) 2388
Mva1269I GAATGC 1 cut(s) 1423
MvaI CCWGG 2 cut(s) 1788, 2213
MwoI GCNNNNNNNGC 7 cut(s) 370, 399, 1417, 1493, 1655, 1753, 2084
NciI CCSGG 1 cut(s) 267
NdeII GATC 8 cut(s) 4, 292, 382, 475, 505, 1445, 1736, 2392
NlaIV GGNNCC 6 cut(s) 6, 165, 1779, 2077, 2372, 2460
NmuCI GTSAC 4 cut(s) 216, 1208, 2098, 2168
NsiI ATGCAT 1 cut(s) 2149
NspI RCATGY 2 cut(s) 929, 2238
PagI TCATGA 2 cut(s) 33, 1063
PctI GAATGC 1 cut(s) 1423
PdmI GAANNNNTTC 1 cut(s) 413
PflFI GACNNNGTC 1 cut(s) 1160
PkrI GCNGC 4 cut(s) 899, 1251, 1422, 1829
PleI GAGTC 4 cut(s) 15, 61, 125, 223
PpsI GAGTC 4 cut(s) 15, 61, 125, 223
PpuMI RGGWCCY 2 cut(s) 1389, 1915
PsiI TTATAA 1 cut(s) 1122
Psp5II RGGWCCY 2 cut(s) 1389, 1915
Psp6I CCWGG 2 cut(s) 1786, 2211
PspGI CCWGG 2 cut(s) 1786, 2211
PspN4I GGNNCC 6 cut(s) 6, 165, 1779, 2077, 2372, 2460
PspPI GGNCC 5 cut(s) 391, 1389, 1745, 1915, 2428
PspPPI RGGWCCY 2 cut(s) 1389, 1915
PstNI CAGNNNCTG 1 cut(s) 784
PsuI RGATCY 3 cut(s) 4, 1445, 1736
PsyI GACNNNGTC 1 cut(s) 1160
RsaI GTAC 5 cut(s) 101, 178, 1501, 2289, 2372
RsaNI GTAC 5 cut(s) 100, 177, 1500, 2288, 2371
RseI CAYNNNNRTG 1 cut(s) 2225
SatI GCNGC 4 cut(s) 898, 1250, 1421, 1828
Sau3AI GATC 8 cut(s) 4, 292, 382, 475, 505, 1445, 1736, 2392
Sau96I GGNCC 5 cut(s) 391, 1389, 1745, 1915, 2428
SchI GAGTC 4 cut(s) 15, 62, 125, 224
ScrFI CCNGG 3 cut(s) 267, 1788, 2213
SduI GDGCHC 3 cut(s) 266, 524, 1363
SfaNI GCATC 4 cut(s) 324, 1280, 2134, 2400
SinI GGWCC 2 cut(s) 1389, 1915
SmiMI CAYNNNNRTG 1 cut(s) 2225
SmlI CTYRAG 2 cut(s) 1481, 1919
SmoI CTYRAG 2 cut(s) 1481, 1919
SsiI CCGC 4 cut(s) 494, 1206, 1367, 1828
SspMI CTAG 4 cut(s) 576, 588, 741, 978
StyD4I CCNGG 3 cut(s) 265, 1786, 2211
TaiI ACGT 1 cut(s) 2293
TaqI TCGA 4 cut(s) 51, 508, 1173, 2104
TauI GCSGC 1 cut(s) 1830
TscAI CASTG 6 cut(s) 22, 409, 676, 909, 1513, 1709
TseFI GTSAC 4 cut(s) 216, 1208, 2098, 2168
TseI GCWGC 3 cut(s) 897, 1249, 1420
Tsp45I GTSAC 4 cut(s) 216, 1208, 2098, 2168
TspGWI ACGGA 4 cut(s) 246, 586, 706, 1522
TspRI CASTG 6 cut(s) 22, 409, 676, 909, 1513, 1709
Tth111I GACNNNGTC 1 cut(s) 1160
VneI GTGCAC 1 cut(s) 1359
VpaK11BI GGWCC 2 cut(s) 1389, 1915
XagI CCTNNNNNAGG 1 cut(s) 231
XapI RAATTY 1 cut(s) 299
XceI RCATGY 2 cut(s) 929, 2238
XmiI GTMKAC 1 cut(s) 1373
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 4 cut(s) 576, 588, 741, 978
Zsp2I ATGCAT 1 cut(s) 2149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.