Rw5G040410

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003720
Physical Location & Seq
Forward (+)
71300989 .. 71317268
16280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G040410.1

Sequence Viewer

Length: 2469 bp
ATGGATCCACCTTACACTGGACTCCCTGATTTCATGAACGACATTGATTTCGAGTCCATGTTACCCAATTCATCTCAATTCCCAGAAATCCCAAATCAGTACCAATTTAACCAGCTCTCTCCAGACCTTGACTCTTTGAGCAATCAGTTTTCAATTCCACCGGAGCCGGTATCCGGTACTTTTGTTCCTCCAATTAGTGTCAGCACAGAAGGAGAGTCAATTCTTCCTAACGGAGGCTCATTTTCTCTTCCTACAACCGTGAGCCCCGGAGTAGATTCTAAGTCCTCTGATGATCTTGAATTCTCTGAAACTGTTTTCAAGTATGTAAACCAGATGCTTATGGAAGAGAACATAGAGGAAAAGCCCCTCATGCTCTATGATGATCCATTGGGCCTTCATGGCACTGAGAAATCGTTCTATGAAGTTCTTGGTCAACAGTATCCCTTCTCACCCAATCAGCAACCACTTTATGATGATCAACCCAATCAGCAACCGCTTTATGTTGATCGAAAAGATGAGAGCCCTGATAGTTACTTTTCTGGTAACAGTAGTGATTTCAATACTGGTAGTAGTCCTAGTGCTGGCACTAGCAACTCCGTTGATTCTCAATCGCTTGGTGATTATGGAGAGCAGAAGCCATCTTTGTCAGAAACCTCTCGTTCAAGGGACAGTGTTTACCAGTTTAATTCTCATTCCAATTCCAATTCGCAGTTGTCCGTTCCTCTGACAAATAGCCTGTCTAGTTTTGGTGGTGGGATGTCTGAATCTTCTGTAAATCAGTTTCTGGCTCAGAATATATTTACAGATAGTGAATCTGTTTTGCAGTTTCAGAGAGGTTTAGAGCAAGCAAGCAAGTTCCTTCCCAAAGTTAATCCATTAGTTATTGATTTGGAAAGCAGCACAGTGTCTCCAGAAGTGAAAGGACATGCTCCAACTGATATCGTAAACAAGGAAAAGAGTGAGAGGAAGAACCGACCTAGTAGGCGAAGGGGAAGGAGGAAATCACCGAATGGGTCAAGGGAAAGGAAGAACTCACCTGATGGGTCAAGGGGGAGGAAGAATCATGAGCGAGAAGATGTGGATTCTGAAGGAGGGAGGAGTAGGAAGCAGTCTGCAATTTATAAGGGGGAGGAGGATGAATTATCTGAGATGTTTGACAAGGTCTTGCTTTTCGACGGGTCTAATCTGTCTTCATGTGGCTCATGCGGCGACAATGTAGATGTGCAGAATGAAGAAAGCAAAAACTTGCAGCTCAATGGACAGCCACAAGCATCAAATGATGAAGGTGGGAAGGCTCGTGCGAAGAAACAAGGCAAGAAGAAGGAAACTGTGGATTTAAGGAATCTTCTGATTCTGTGTGCACAAGCGGTGTCTACCAATGATTTTAGGACCTCCACAGAACTACTAAAGCAAATTAGGCAGCATTCTTCTCCTTTTGGTGATGGATCTCAAAGGTTGGCTCATTTCTTTGCAAATGGCCTTGAGGCACGAATGGCTGGTACTGGCACTGGAAACCAGTTTTATTATATTTCCGTTGCTTCCAAACAGAAGGCTGTTGATAAGTTGAAATGTTACCAAGTTCATCTTTCAGCTTGCCCTTTTAAGAGAATGTCAATGTTCTTCAAAAACAAGATGATTTTCAAGATGGCTGAGAAAGCAACTACCCTCCATATTGTAGATTTTGGTATCCTGTATGGTTTTCAGTGGCCAATCCTTATCCACAAGCTTTCAACGAGACCTGGTGGGCCTCCCAAGCTACGAATTACAGGGATAGAGGTTCCCCAACCTGGATTTCGTCCAGCAGAGTGGATTGAAGAGACTGGACGCCGCTTGGCGAGATATTGCAAGCGCTTTAATGTTCCTTTTGAGTTTAATGCAATAGCCTCGCAGAATTGGGAATCCATCCAAGTAGAGGACCTCAAGGTTGAGAGGAATGAGGTGCTTGCTGTGAATTGTATGTTACGGTTTAAGAACCTACTGGATGAGACTGTTGAAGTAAACTGTCCAAGAGATTCTGTTCTAAAGCTAATCAGGAGGTTGAAACCAGATATTTTTGTTCATACTATTGTTAATGGTGCCTACAATGCCCCCTTCTTTGTCACTCGATTTCGGGAGGCCCTCTTCCACTTCTCTGCCTTGTATGATGTATTTGATGTTAATATACCCCGTGACAGTTCAGAGAGGTTGATGTTTGAATCAGAGTTCTATGGCCGGGAGGCCATGAATGTGATAGCATGTGAGGGGATAGAGAGGGTTGAGAGAGCTGAGACATACAAGCAATGGCAGGTACGTTGTCAAAGGGCTGGTTTACAGTTGTTGCCATTGGACCAAGAGGTAGTGAAGGTGTTTAGGGATAAGGTGAAGGCATGGTACCACAAAGATTTCGCAATTGATCAAGATAGTGATTGGATGCTTCAAGGATGGAAGGGTCGAATTGTCTATGCTTCTTCTTGTTGGGTGCCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

822

Amino Acids

92.78

Weight (kDa)

5.64

Isoelectric Point (pI)

54.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 446 - 819 9.7e-115 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1122
Acc36I ACCTGC 1 cut(s) 2275
Acc65I GGTACC 1 cut(s) 2370
AccB1I GGYRCC 3 cut(s) 2075, 2370, 2458
AccI GTMKAC 1 cut(s) 1373
AciI CCGC 4 cut(s) 494, 1206, 1367, 1828
AclWI GGATC 3 cut(s) 12, 377, 1453
AcoI YGGCCR 2 cut(s) 1706, 2209
AcsI RAATTY 1 cut(s) 299
AcuI CTGAAG 1 cut(s) 1107
AcyI GRCGYC 1 cut(s) 1825
AfaI GTAC 5 cut(s) 101, 178, 1501, 2289, 2372
AfeI AGCGCT 1 cut(s) 1850
AfiI CCNNNNNNNGG 6 cut(s) 17, 173, 233, 581, 1787, 1912
AjnI CCWGG 2 cut(s) 1738, 1786
AjuI GAANNNNNNNTTGG 2 cut(s) 1776, 1808
AluBI AGCT 7 cut(s) 115, 1252, 1592, 1726, 1756, 2026, 2264
AluI AGCT 7 cut(s) 115, 1252, 1592, 1726, 1756, 2026, 2264
Alw21I GWGCWC 1 cut(s) 1363
Alw26I GTCTC 5 cut(s) 912, 1729, 1811, 1979, 2261
Alw44I GTGCAC 1 cut(s) 1359
AlwI GGATC 3 cut(s) 12, 377, 1453
AlwNI CAGNNNCTG 1 cut(s) 784
Aor51HI AGCGCT 1 cut(s) 1850
AoxI GGCC 7 cut(s) 391, 1477, 1706, 1745, 2115, 2209, 2217
ApaLI GTGCAC 1 cut(s) 1359
ApeKI GCWGC 3 cut(s) 897, 1249, 1420
ApoI RAATTY 1 cut(s) 299
ArsI GACNNNNNNTTYG 4 cut(s) 32, 64, 722, 754
Asp700I GAANNNNTTC 1 cut(s) 413
Asp718I GGTACC 1 cut(s) 2370
AspLEI GCGC 1 cut(s) 1851
AspS9I GGNCC 6 cut(s) 391, 1389, 1745, 1915, 2116, 2326
AsuC2I CCSGG 2 cut(s) 267, 2213
AsuHPI GGTGA 6 cut(s) 441, 629, 996, 1026, 1451, 2371
AvaII GGWCC 3 cut(s) 1389, 1915, 2326
BaeGI GKGCMC 1 cut(s) 1363
BaeI ACNNNNGTAYC 2 cut(s) 168, 201
BalI TGGCCA 1 cut(s) 1708
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 3 cut(s) 2075, 2370, 2458
BanII GRGCYC 2 cut(s) 266, 524
BarI GAAGNNNNNNTAC 2 cut(s) 2354, 2386
BauI CACGAG 1 cut(s) 1296
BbsI GAAGAC 1 cut(s) 1182
Bbv12I GWGCWC 1 cut(s) 1363
BbvI GCAGC 3 cut(s) 909, 1261, 1432
BccI CCATC 6 cut(s) 646, 1034, 1436, 1639, 1910, 2415
BcgI CGANNNNNNTGC 2 cut(s) 1866, 1900
BciT130I CCWGG 2 cut(s) 1740, 1788
BciVI GTATCC 3 cut(s) 181, 450, 1697
BclI TGATCA 2 cut(s) 475, 2392
BcnI CCSGG 2 cut(s) 267, 2213
BcoDI GTCTC 5 cut(s) 912, 1729, 1811, 1979, 2261
BfaI CTAG 4 cut(s) 576, 588, 741, 978
BfoI RGCGCY 1 cut(s) 1852
BfuAI ACCTGC 1 cut(s) 2275
BfuI GTATCC 3 cut(s) 181, 450, 1697
BglI GCCNNNNNGGC 1 cut(s) 399
BisI GCNGC 5 cut(s) 898, 1207, 1250, 1421, 1828
BlsI GCNGC 5 cut(s) 899, 1208, 1251, 1422, 1829
Bme1390I CCNGG 4 cut(s) 267, 1740, 1788, 2213
Bme18I GGWCC 3 cut(s) 1389, 1915, 2326
BmgT120I GGNCC 6 cut(s) 391, 1389, 1745, 1915, 2116, 2326
BmiI GGNNCC 6 cut(s) 6, 165, 1779, 2077, 2372, 2460
BmrFI CCNGG 4 cut(s) 267, 1740, 1788, 2213
BmsI GCATC 3 cut(s) 324, 1280, 2400
BpiI GAAGAC 1 cut(s) 1182
BpmI CTGGAG 2 cut(s) 105, 894
BpuEI CTTGAG 2 cut(s) 1502, 1904
BpuMI CCSGG 2 cut(s) 267, 2213
BsaHI GRCGYC 1 cut(s) 1825
BsaI GGTCTC 1 cut(s) 1729
BsaJI CCNNGG 1 cut(s) 265
BsaWI WCCGGW 2 cut(s) 160, 173
BsaXI ACNNNNNCTCC 2 cut(s) 892, 922
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 173, 233, 581, 1787, 1912
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 8 cut(s) 22, 568, 679, 1507, 1513, 1516, 1825, 1983
Bse3DI GCAATG 1 cut(s) 2285
BseBI CCWGG 2 cut(s) 1740, 1788
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 6 cut(s) 762, 1141, 1902, 1987, 2415, 2426
BseLI CCNNNNNNNGG 6 cut(s) 17, 173, 233, 581, 1787, 1912
BseMI GCAATG 1 cut(s) 2285
BseMII CTCAG 5 cut(s) 396, 803, 1137, 1641, 2256
BseNI ACTGG 8 cut(s) 22, 568, 679, 1507, 1513, 1516, 1825, 1983
BseRI GAGGAG 2 cut(s) 1111, 1145
BseSI GKGCMC 1 cut(s) 1363
BseXI GCAGC 3 cut(s) 909, 1261, 1432
BsgI GTGCAG 1 cut(s) 1244
BshFI GGCC 7 cut(s) 393, 1479, 1708, 1747, 2117, 2211, 2219
BshNI GGYRCC 3 cut(s) 2075, 2370, 2458
BsiHKAI GWGCWC 1 cut(s) 1363
BsiSI CCGG 5 cut(s) 161, 167, 174, 267, 2212
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 6 cut(s) 17, 173, 233, 581, 1787, 1912
BsmAI GTCTC 5 cut(s) 912, 1729, 1811, 1979, 2261
BsmFI GGGAC 1 cut(s) 680
BsmI GAATGC 1 cut(s) 1423
BsnI GGCC 7 cut(s) 393, 1479, 1708, 1747, 2117, 2211, 2219
Bso31I GGTCTC 1 cut(s) 1729
Bsp1286I GDGCHC 3 cut(s) 266, 524, 1363
Bsp143I GATC 7 cut(s) 4, 292, 382, 475, 505, 1445, 2392
BspACI CCGC 4 cut(s) 494, 1206, 1367, 1828
BspANI GGCC 7 cut(s) 393, 1479, 1708, 1747, 2117, 2211, 2219
BspCNI CTCAG 5 cut(s) 397, 802, 1138, 1642, 2257
BspHI TCATGA 2 cut(s) 33, 1063
BspLI GGNNCC 6 cut(s) 6, 165, 1779, 2077, 2372, 2460
BspMI ACCTGC 1 cut(s) 2275
BspPI GGATC 3 cut(s) 12, 377, 1453
BspT107I GGYRCC 3 cut(s) 2075, 2370, 2458
BspTNI GGTCTC 1 cut(s) 1729
BsrDI GCAATG 1 cut(s) 2285
BsrFI RCCGGY 1 cut(s) 166
BsrI ACTGG 8 cut(s) 22, 568, 679, 1507, 1513, 1516, 1825, 1983
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 7 cut(s) 4, 292, 382, 475, 505, 1445, 2392
BssNI GRCGYC 1 cut(s) 1825
BssSI CACGAG 1 cut(s) 1296
Bst2BI CACGAG 1 cut(s) 1296
Bst2UI CCWGG 2 cut(s) 1740, 1788
Bst6I CTCTTC 4 cut(s) 252, 339, 1809, 2126
BstACI GRCGYC 1 cut(s) 1825
BstC8I GCNNGC 7 cut(s) 583, 846, 850, 1594, 1847, 1944, 2463
BstDEI CTNAG 6 cut(s) 279, 405, 789, 1146, 1650, 2265
BstENI CCTNNNNNAGG 1 cut(s) 231
BstF5I GGATG 6 cut(s) 762, 1141, 1902, 1987, 2415, 2426
BstH2I RGCGCY 1 cut(s) 1852
BstHHI GCGC 1 cut(s) 1851
BstKTI GATC 7 cut(s) 7, 295, 385, 478, 508, 1448, 2395
BstMAI GTCTC 5 cut(s) 912, 1729, 1811, 1979, 2261
BstMBI GATC 7 cut(s) 4, 292, 382, 475, 505, 1445, 2392
BstMWI GCNNNNNNNGC 8 cut(s) 370, 399, 1206, 1417, 1493, 1655, 1753, 2084
BstNI CCWGG 2 cut(s) 1740, 1788
BstNSI RCATGY 2 cut(s) 929, 2238
BstSCI CCNGG 4 cut(s) 265, 1738, 1786, 2211
BstSLI GKGCMC 1 cut(s) 1363
BstV1I GCAGC 3 cut(s) 909, 1261, 1432
BstV2I GAAGAC 1 cut(s) 1182
BstX2I RGATCY 2 cut(s) 4, 1445
BstXI CCANNNNNNTGG 1 cut(s) 1806
BstYI RGATCY 2 cut(s) 4, 1445
BsuI GTATCC 3 cut(s) 181, 450, 1697
BsuRI GGCC 7 cut(s) 393, 1479, 1708, 1747, 2117, 2211, 2219
BtsCI GGATG 6 cut(s) 762, 1141, 1902, 1987, 2415, 2426
BtsIMutI CAGTG 6 cut(s) 15, 402, 676, 909, 1506, 1709
BveI ACCTGC 1 cut(s) 2275
Cac8I GCNNGC 7 cut(s) 583, 846, 850, 1594, 1847, 1944, 2463
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 2 cut(s) 33, 1063
CfoI GCGC 1 cut(s) 1851
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 6 cut(s) 391, 1389, 1745, 1915, 2116, 2326
CseI GACGC 1 cut(s) 1833
CsiI ACCWGGT 1 cut(s) 1738
Csp6I GTAC 5 cut(s) 100, 177, 1500, 2288, 2371
CviQI GTAC 5 cut(s) 100, 177, 1500, 2288, 2371
DdeI CTNAG 6 cut(s) 279, 405, 789, 1146, 1650, 2265
DpnI GATC 7 cut(s) 6, 294, 384, 477, 507, 1447, 2394
DpnII GATC 7 cut(s) 4, 292, 382, 475, 505, 1445, 2392
EaeI YGGCCR 2 cut(s) 1706, 2209
Eam1104I CTCTTC 4 cut(s) 252, 339, 1809, 2126
EarI CTCTTC 4 cut(s) 252, 339, 1809, 2126
Eco24I GRGCYC 2 cut(s) 266, 524
Eco31I GGTCTC 1 cut(s) 1729
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 3 cut(s) 1389, 1915, 2326
Eco47III AGCGCT 1 cut(s) 1850
Eco57I CTGAAG 1 cut(s) 1107
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoO109I RGGNCCY 3 cut(s) 1389, 1915, 2116
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 1738, 1786
EcoRV GATATC 1 cut(s) 940
EcoT38I GRGCYC 2 cut(s) 266, 524
FalI AAGNNNNNCTT 2 cut(s) 1569, 1601
FaqI GGGAC 1 cut(s) 680
FbaI TGATCA 2 cut(s) 475, 2392
FblI GTMKAC 1 cut(s) 1373
Fnu4HI GCNGC 5 cut(s) 898, 1207, 1250, 1421, 1828
FokI GGATG 6 cut(s) 769, 1148, 1889, 1994, 2422, 2433
FriOI GRGCYC 2 cut(s) 266, 524
Fsp4HI GCNGC 5 cut(s) 898, 1207, 1250, 1421, 1828
FspBI CTAG 4 cut(s) 576, 588, 741, 978
GlaI GCGC 1 cut(s) 1850
GluI GCNGC 5 cut(s) 898, 1207, 1250, 1421, 1828
GsuI CTGGAG 2 cut(s) 105, 894
HaeII RGCGCY 1 cut(s) 1852
HaeIII GGCC 7 cut(s) 393, 1479, 1708, 1747, 2117, 2211, 2219
HapII CCGG 5 cut(s) 161, 167, 174, 267, 2212
HgaI GACGC 1 cut(s) 1833
HhaI GCGC 1 cut(s) 1851
Hin1I GRCGYC 1 cut(s) 1825
Hin6I GCGC 1 cut(s) 1849
HinP1I GCGC 1 cut(s) 1849
HincII GTYRAC 1 cut(s) 434
HindII GTYRAC 1 cut(s) 434
HindIII AAGCTT 1 cut(s) 1724
HpaII CCGG 5 cut(s) 161, 167, 174, 267, 2212
HphI GGTGA 6 cut(s) 441, 629, 996, 1026, 1451, 2371
Hpy166II GTNNAC 8 cut(s) 328, 434, 676, 946, 1361, 1374, 1999, 2309
Hpy188III TCNNGA 9 cut(s) 34, 122, 296, 911, 1064, 1642, 2032, 2111, 2396
Hpy8I GTNNAC 8 cut(s) 328, 434, 676, 946, 1361, 1374, 1999, 2309
Hpy99I CGWCG 1 cut(s) 1178
HpyCH4IV ACGT 1 cut(s) 2290
HpyCH4V TGCA 9 cut(s) 823, 1115, 1225, 1249, 1361, 1472, 1845, 1877, 2465
HpyF10VI GCNNNNNNNGC 8 cut(s) 370, 399, 1206, 1417, 1493, 1655, 1753, 2084
HpyF3I CTNAG 6 cut(s) 279, 405, 789, 1146, 1650, 2265
HpySE526I ACGT 1 cut(s) 2290
Hsp92I GRCGYC 1 cut(s) 1825
HspAI GCGC 1 cut(s) 1849
KpnI GGTACC 1 cut(s) 2374
Ksp22I TGATCA 2 cut(s) 475, 2392
Kzo9I GATC 7 cut(s) 4, 292, 382, 475, 505, 1445, 2392
LmnI GCTCC 2 cut(s) 163, 934
Lsp1109I GCAGC 3 cut(s) 909, 1261, 1432
LweI GCATC 3 cut(s) 324, 1280, 2400
MabI ACCWGGT 1 cut(s) 1738
MaeI CTAG 4 cut(s) 576, 588, 741, 978
MaeII ACGT 1 cut(s) 2290
MaeIII GTNAC 7 cut(s) 60, 530, 542, 1571, 1959, 2098, 2168
MalI GATC 7 cut(s) 6, 294, 384, 477, 507, 1447, 2394
MboI GATC 7 cut(s) 4, 292, 382, 475, 505, 1445, 2392
MfeI CAATTG 1 cut(s) 2388
MflI RGATCY 2 cut(s) 4, 1445
MhlI GDGCHC 3 cut(s) 266, 524, 1363
MlsI TGGCCA 1 cut(s) 1708
MluNI TGGCCA 1 cut(s) 1708
MlyI GAGTC 4 cut(s) 15, 62, 125, 224
MmeI TCCRAC 1 cut(s) 956
Mox20I TGGCCA 1 cut(s) 1708
MroXI GAANNNNTTC 1 cut(s) 413
MscI TGGCCA 1 cut(s) 1708
MslI CAYNNNNRTG 1 cut(s) 2225
Msp20I TGGCCA 1 cut(s) 1708
MspI CCGG 5 cut(s) 161, 167, 174, 267, 2212
MspR9I CCNGG 4 cut(s) 267, 1740, 1788, 2213
MunI CAATTG 1 cut(s) 2388
Mva1269I GAATGC 1 cut(s) 1423
MvaI CCWGG 2 cut(s) 1740, 1788
MwoI GCNNNNNNNGC 8 cut(s) 370, 399, 1206, 1417, 1493, 1655, 1753, 2084
NciI CCSGG 2 cut(s) 267, 2213
NdeII GATC 7 cut(s) 4, 292, 382, 475, 505, 1445, 2392
NlaIV GGNNCC 6 cut(s) 6, 165, 1779, 2077, 2372, 2460
NmuCI GTSAC 2 cut(s) 2098, 2168
NspI RCATGY 2 cut(s) 929, 2238
PagI TCATGA 2 cut(s) 33, 1063
PctI GAATGC 1 cut(s) 1423
PdmI GAANNNNTTC 1 cut(s) 413
PflFI GACNNNGTC 1 cut(s) 1160
PkrI GCNGC 5 cut(s) 899, 1208, 1251, 1422, 1829
PleI GAGTC 4 cut(s) 15, 61, 125, 223
PpsI GAGTC 4 cut(s) 15, 61, 125, 223
PpuMI RGGWCCY 2 cut(s) 1389, 1915
PsiI TTATAA 1 cut(s) 1122
Psp5II RGGWCCY 2 cut(s) 1389, 1915
Psp6I CCWGG 2 cut(s) 1738, 1786
PspGI CCWGG 2 cut(s) 1738, 1786
PspN4I GGNNCC 6 cut(s) 6, 165, 1779, 2077, 2372, 2460
PspPI GGNCC 6 cut(s) 391, 1389, 1745, 1915, 2116, 2326
PspPPI RGGWCCY 2 cut(s) 1389, 1915
PstNI CAGNNNCTG 1 cut(s) 784
PsuI RGATCY 2 cut(s) 4, 1445
PsyI GACNNNGTC 1 cut(s) 1160
RsaI GTAC 5 cut(s) 101, 178, 1501, 2289, 2372
RsaNI GTAC 5 cut(s) 100, 177, 1500, 2288, 2371
RseI CAYNNNNRTG 1 cut(s) 2225
SatI GCNGC 5 cut(s) 898, 1207, 1250, 1421, 1828
Sau3AI GATC 7 cut(s) 4, 292, 382, 475, 505, 1445, 2392
Sau96I GGNCC 6 cut(s) 391, 1389, 1745, 1915, 2116, 2326
SchI GAGTC 4 cut(s) 15, 62, 125, 224
ScrFI CCNGG 4 cut(s) 267, 1740, 1788, 2213
SduI GDGCHC 3 cut(s) 266, 524, 1363
SexAI ACCWGGT 1 cut(s) 1738
SfaNI GCATC 3 cut(s) 324, 1280, 2400
SinI GGWCC 3 cut(s) 1389, 1915, 2326
SmiMI CAYNNNNRTG 1 cut(s) 2225
SmlI CTYRAG 2 cut(s) 1481, 1919
SmoI CTYRAG 2 cut(s) 1481, 1919
SsiI CCGC 4 cut(s) 494, 1206, 1367, 1828
SspMI CTAG 4 cut(s) 576, 588, 741, 978
StyD4I CCNGG 4 cut(s) 265, 1738, 1786, 2211
TaiI ACGT 1 cut(s) 2293
TaqI TCGA 5 cut(s) 51, 508, 1173, 2104, 2431
TauI GCSGC 2 cut(s) 1209, 1830
TscAI CASTG 6 cut(s) 22, 409, 676, 909, 1513, 1709
TseFI GTSAC 2 cut(s) 2098, 2168
TseI GCWGC 3 cut(s) 897, 1249, 1420
Tsp45I GTSAC 2 cut(s) 2098, 2168
TspGWI ACGGA 4 cut(s) 246, 586, 706, 1522
TspRI CASTG 6 cut(s) 22, 409, 676, 909, 1513, 1709
Tth111I GACNNNGTC 1 cut(s) 1160
VneI GTGCAC 1 cut(s) 1359
VpaK11BI GGWCC 3 cut(s) 1389, 1915, 2326
XagI CCTNNNNNAGG 1 cut(s) 231
XapI RAATTY 1 cut(s) 299
XceI RCATGY 2 cut(s) 929, 2238
XmiI GTMKAC 1 cut(s) 1373
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 4 cut(s) 576, 588, 741, 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.