mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In the eIF-3 complex, eif3d specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G20980 AT4G20980 AT4G20980 AT4G20980 AT5G44320 |
| fragaria_vesca | FvH4_3g37950 FvH4_5g27490 |
| malus_domestica | MD05G1338100.v1.1 |
| prunus_persica | Prupe.4G048700_v2.0.a1 |
| pyrus_communis | pycom10g24520 |
| rosa_chinensis | RchiOBHm_Chr5g0048541 |
| rosa_laevigata | RLG00000034531 |
| rosa_multiflora | Rmu_sc0007341.1_g000041 |
| rosa_roxburghii | Rroxscaffold_1G00033270 |
| rosa_rugosa | Rorug05G0239400 |
| rosa_samantha | Rh5AG319100 Rh5BG329500 Rh5CG355200 Rh5DG340700 |
| rosa_wichuraiana | Rw0G021230 Rw5G029840 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 754 |
| Acc36I | ACCTGC | 1 cut(s) | 949 |
| AccB1I | GGYRCC | 2 cut(s) | 557, 1531 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1428 |
| AccBSI | CCGCTC | 3 cut(s) | 102, 159, 622 |
| AccI | GTMKAC | 3 cut(s) | 249, 414, 768 |
| AccII | CGCG | 3 cut(s) | 268, 327, 354 |
| AcoI | YGGCCR | 2 cut(s) | 115, 1230 |
| AcsI | RAATTY | 3 cut(s) | 275, 946, 1163 |
| AcuI | CTGAAG | 2 cut(s) | 1490, 1626 |
| AcyI | GRCGYC | 2 cut(s) | 27, 177 |
| AfaI | GTAC | 1 cut(s) | 1488 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 48, 278, 291, 331, 583, 1428 |
| AflII | CTTAAG | 1 cut(s) | 1241 |
| AgsI | TTSAA | 7 cut(s) | 37, 145, 631, 1326, 1351, 1550, 1667 |
| AjnI | CCWGG | 2 cut(s) | 284, 561 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1599, 1631 |
| Alw26I | GTCTC | 3 cut(s) | 854, 1086, 1202 |
| Ama87I | CYCGRG | 3 cut(s) | 530, 617, 792 |
| AoxI | GGCC | 8 cut(s) | 52, 115, 280, 342, 357, 708, 1230, 1419 |
| ApaI | GGGCCC | 1 cut(s) | 56 |
| ApeKI | GCWGC | 4 cut(s) | 301, 929, 1031, 1637 |
| ApoI | RAATTY | 3 cut(s) | 275, 946, 1163 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 167, 199 |
| AseI | ATTAAT | 1 cut(s) | 1407 |
| AspA2I | CCTAGG | 1 cut(s) | 758 |
| AspLEI | GCGC | 5 cut(s) | 270, 354, 612, 755, 1156 |
| AspS9I | GGNCC | 6 cut(s) | 4, 52, 53, 132, 281, 1272 |
| AsuHPI | GGTGA | 5 cut(s) | 311, 514, 799, 979, 1504 |
| AvaI | CYCGRG | 3 cut(s) | 530, 617, 792 |
| AvaII | GGWCC | 3 cut(s) | 4, 132, 1272 |
| AvrII | CCTAGG | 1 cut(s) | 758 |
| AxyI | CCTNAGG | 1 cut(s) | 537 |
| BaeGI | GKGCMC | 2 cut(s) | 56, 562 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1647, 1680 |
| BalI | TGGCCA | 1 cut(s) | 1232 |
| BamHI | GGATCC | 1 cut(s) | 1498 |
| BanI | GGYRCC | 2 cut(s) | 557, 1531 |
| BanII | GRGCYC | 2 cut(s) | 56, 376 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1557, 1589 |
| BauI | CACGAG | 1 cut(s) | 1338 |
| BbsI | GAAGAC | 2 cut(s) | 175, 708 |
| BbvI | GCAGC | 4 cut(s) | 313, 916, 1018, 1624 |
| BccI | CCATC | 5 cut(s) | 160, 821, 968, 1510, 1604 |
| BceAI | ACGGC | 4 cut(s) | 164, 363, 411, 422 |
| BciT130I | CCWGG | 2 cut(s) | 286, 563 |
| BciVI | GTATCC | 2 cut(s) | 1370, 1648 |
| BcoDI | GTCTC | 3 cut(s) | 854, 1086, 1202 |
| BfaI | CTAG | 1 cut(s) | 759 |
| BfrI | CTTAAG | 1 cut(s) | 1241 |
| BfuAI | ACCTGC | 1 cut(s) | 949 |
| BfuI | GTATCC | 2 cut(s) | 1370, 1648 |
| BisI | GCNGC | 9 cut(s) | 25, 266, 302, 325, 435, 688, 930, 1032, 1638 |
| BlnI | CCTAGG | 1 cut(s) | 758 |
| BlsI | GCNGC | 9 cut(s) | 26, 267, 303, 326, 436, 689, 931, 1033, 1639 |
| Bme1390I | CCNGG | 2 cut(s) | 286, 563 |
| Bme18I | GGWCC | 3 cut(s) | 4, 132, 1272 |
| BmeT110I | CYCGRG | 3 cut(s) | 530, 617, 792 |
| BmgT120I | GGNCC | 6 cut(s) | 4, 52, 53, 132, 281, 1272 |
| BmiI | GGNNCC | 9 cut(s) | 53, 54, 134, 283, 296, 559, 873, 1500, 1533 |
| BmrFI | CCNGG | 2 cut(s) | 286, 563 |
| BmsI | GCATC | 1 cut(s) | 1531 |
| BpiI | GAAGAC | 2 cut(s) | 175, 708 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1382, 1414 |
| BpmI | CTGGAG | 1 cut(s) | 584 |
| BsaHI | GRCGYC | 2 cut(s) | 27, 177 |
| BsaI | GGTCTC | 1 cut(s) | 854 |
| BsaWI | WCCGGW | 1 cut(s) | 58 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 215, 245, 1466, 1496 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 48, 278, 291, 331, 583, 1428 |
| Bse1I | ACTGG | 4 cut(s) | 134, 677, 1422, 1494 |
| Bse21I | CCTNAGG | 1 cut(s) | 537 |
| BseBI | CCWGG | 2 cut(s) | 286, 563 |
| BseGI | GGATG | 3 cut(s) | 152, 681, 1333 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 48, 278, 291, 331, 583, 1428 |
| BseMII | CTCAG | 4 cut(s) | 528, 881, 908, 1226 |
| BseNI | ACTGG | 4 cut(s) | 134, 677, 1422, 1494 |
| BseRI | GAGGAG | 2 cut(s) | 347, 1010 |
| BseSI | GKGCMC | 2 cut(s) | 56, 562 |
| BseX3I | CGGCCG | 1 cut(s) | 115 |
| BseXI | GCAGC | 4 cut(s) | 313, 916, 1018, 1624 |
| BseYI | CCCAGC | 1 cut(s) | 1312 |
| BsgI | GTGCAG | 1 cut(s) | 1641 |
| Bsh1236I | CGCG | 3 cut(s) | 268, 327, 354 |
| Bsh1285I | CGRYCG | 1 cut(s) | 118 |
| BshFI | GGCC | 8 cut(s) | 54, 117, 282, 344, 359, 710, 1232, 1421 |
| BshNI | GGYRCC | 2 cut(s) | 557, 1531 |
| BsiEI | CGRYCG | 1 cut(s) | 118 |
| BsiHKCI | CYCGRG | 3 cut(s) | 530, 617, 792 |
| BsiSI | CCGG | 3 cut(s) | 59, 118, 467 |
| BslFI | GGGAC | 4 cut(s) | 14, 65, 512, 791 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 48, 278, 291, 331, 583, 1428 |
| BsmAI | GTCTC | 3 cut(s) | 854, 1086, 1202 |
| BsmFI | GGGAC | 4 cut(s) | 14, 65, 512, 791 |
| BsmI | GAATGC | 1 cut(s) | 1156 |
| BsnI | GGCC | 8 cut(s) | 54, 117, 282, 344, 359, 710, 1232, 1421 |
| Bso31I | GGTCTC | 1 cut(s) | 854 |
| BsoBI | CYCGRG | 3 cut(s) | 530, 617, 792 |
| Bsp120I | GGGCCC | 1 cut(s) | 52 |
| Bsp1286I | GDGCHC | 3 cut(s) | 56, 376, 562 |
| BspANI | GGCC | 8 cut(s) | 54, 117, 282, 344, 359, 710, 1232, 1421 |
| BspCNI | CTCAG | 4 cut(s) | 529, 880, 909, 1225 |
| BspFNI | CGCG | 3 cut(s) | 268, 327, 354 |
| BspLI | GGNNCC | 9 cut(s) | 53, 54, 134, 283, 296, 559, 873, 1500, 1533 |
| BspMI | ACCTGC | 1 cut(s) | 949 |
| BspT107I | GGYRCC | 2 cut(s) | 557, 1531 |
| BspTI | CTTAAG | 1 cut(s) | 1241 |
| BspTNI | GGTCTC | 1 cut(s) | 854 |
| BsrBI | CCGCTC | 3 cut(s) | 102, 159, 622 |
| BsrI | ACTGG | 4 cut(s) | 134, 677, 1422, 1494 |
| BssNAI | GTATAC | 1 cut(s) | 769 |
| BssNI | GRCGYC | 2 cut(s) | 27, 177 |
| BssSI | CACGAG | 1 cut(s) | 1338 |
| BssT1I | CCWWGG | 6 cut(s) | 7, 648, 758, 888, 990, 1386 |
| Bst1107I | GTATAC | 1 cut(s) | 769 |
| Bst2BI | CACGAG | 1 cut(s) | 1338 |
| Bst2UI | CCWGG | 2 cut(s) | 286, 563 |
| Bst4CI | ACNGT | 4 cut(s) | 47, 526, 583, 715 |
| Bst6I | CTCTTC | 1 cut(s) | 554 |
| BstACI | GRCGYC | 2 cut(s) | 27, 177 |
| BstAFI | CTTAAG | 1 cut(s) | 1241 |
| BstC8I | GCNNGC | 3 cut(s) | 376, 614, 1590 |
| BstDEI | CTNAG | 5 cut(s) | 537, 867, 917, 1212, 1506 |
| BstDSI | CCRYGG | 1 cut(s) | 582 |
| BstEII | GGTNACC | 1 cut(s) | 985 |
| BstF5I | GGATG | 3 cut(s) | 152, 681, 1333 |
| BstFNI | CGCG | 3 cut(s) | 268, 327, 354 |
| BstHHI | GCGC | 5 cut(s) | 270, 354, 612, 755, 1156 |
| BstMAI | GTCTC | 3 cut(s) | 854, 1086, 1202 |
| BstMCI | CGRYCG | 1 cut(s) | 118 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 310, 384, 576, 844, 1040, 1229, 1289 |
| BstNI | CCWGG | 2 cut(s) | 286, 563 |
| BstNSI | RCATGY | 2 cut(s) | 421, 481 |
| BstPI | GGTNACC | 1 cut(s) | 985 |
| BstSCI | CCNGG | 2 cut(s) | 284, 561 |
| BstSLI | GKGCMC | 2 cut(s) | 56, 562 |
| BstUI | CGCG | 3 cut(s) | 268, 327, 354 |
| BstV1I | GCAGC | 4 cut(s) | 313, 916, 1018, 1624 |
| BstV2I | GAAGAC | 2 cut(s) | 175, 708 |
| BstX2I | RGATCY | 3 cut(s) | 489, 541, 1498 |
| BstYI | RGATCY | 3 cut(s) | 489, 541, 1498 |
| BstZ17I | GTATAC | 1 cut(s) | 769 |
| BstZI | CGGCCG | 1 cut(s) | 115 |
| Bsu36I | CCTNAGG | 1 cut(s) | 537 |
| BsuI | GTATCC | 2 cut(s) | 1370, 1648 |
| BsuRI | GGCC | 8 cut(s) | 54, 117, 282, 344, 359, 710, 1232, 1421 |
| BtgI | CCRYGG | 1 cut(s) | 582 |
| BtsCI | GGATG | 3 cut(s) | 152, 681, 1333 |
| BtsI | GCAGTG | 1 cut(s) | 875 |
| BtsIMutI | CAGTG | 3 cut(s) | 711, 875, 914 |
| BveI | ACCTGC | 1 cut(s) | 949 |
| Cac8I | GCNNGC | 3 cut(s) | 376, 614, 1590 |
| CfoI | GCGC | 5 cut(s) | 270, 354, 612, 755, 1156 |
| Cfr13I | GGNCC | 6 cut(s) | 4, 52, 53, 132, 281, 1272 |
| CseI | GACGC | 2 cut(s) | 16, 185 |
| Csp6I | GTAC | 1 cut(s) | 1487 |
| CviAII | CATG | 5 cut(s) | 418, 478, 748, 857, 1355 |
| CviQI | GTAC | 1 cut(s) | 1487 |
| DdeI | CTNAG | 5 cut(s) | 537, 867, 917, 1212, 1506 |
| EaeI | YGGCCR | 2 cut(s) | 115, 1230 |
| EagI | CGGCCG | 1 cut(s) | 115 |
| Eam1104I | CTCTTC | 1 cut(s) | 554 |
| EarI | CTCTTC | 1 cut(s) | 554 |
| EciI | GGCGGA | 4 cut(s) | 230, 490, 674, 730 |
| EclXI | CGGCCG | 1 cut(s) | 115 |
| Eco130I | CCWWGG | 6 cut(s) | 7, 648, 758, 888, 990, 1386 |
| Eco24I | GRGCYC | 2 cut(s) | 56, 376 |
| Eco31I | GGTCTC | 1 cut(s) | 854 |
| Eco32I | GATATC | 2 cut(s) | 460, 898 |
| Eco47I | GGWCC | 3 cut(s) | 4, 132, 1272 |
| Eco52I | CGGCCG | 1 cut(s) | 115 |
| Eco57I | CTGAAG | 2 cut(s) | 1490, 1626 |
| Eco81I | CCTNAGG | 1 cut(s) | 537 |
| Eco88I | CYCGRG | 3 cut(s) | 530, 617, 792 |
| Eco91I | GGTNACC | 1 cut(s) | 985 |
| EcoO109I | RGGNCCY | 1 cut(s) | 52 |
| EcoO65I | GGTNACC | 1 cut(s) | 985 |
| EcoRII | CCWGG | 2 cut(s) | 284, 561 |
| EcoRV | GATATC | 2 cut(s) | 460, 898 |
| EcoT14I | CCWWGG | 6 cut(s) | 7, 648, 758, 888, 990, 1386 |
| EcoT38I | GRGCYC | 2 cut(s) | 56, 376 |
| ErhI | CCWWGG | 6 cut(s) | 7, 648, 758, 888, 990, 1386 |
| FaeI | CATG | 5 cut(s) | 421, 481, 751, 860, 1358 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 503, 535, 797, 829, 974, 1006 |
| FaqI | GGGAC | 4 cut(s) | 14, 65, 512, 791 |
| FatI | CATG | 5 cut(s) | 417, 477, 747, 856, 1354 |
| FauI | CCCGC | 4 cut(s) | 107, 143, 164, 270 |
| FblI | GTMKAC | 3 cut(s) | 249, 414, 768 |
| Fnu4HI | GCNGC | 9 cut(s) | 25, 266, 302, 325, 435, 688, 930, 1032, 1638 |
| FokI | GGATG | 3 cut(s) | 139, 668, 1320 |
| FriOI | GRGCYC | 2 cut(s) | 56, 376 |
| Fsp4HI | GCNGC | 9 cut(s) | 25, 266, 302, 325, 435, 688, 930, 1032, 1638 |
| FspAI | RTGCGCAY | 1 cut(s) | 754 |
| FspBI | CTAG | 1 cut(s) | 759 |
| FspI | TGCGCA | 1 cut(s) | 754 |
| GlaI | GCGC | 5 cut(s) | 269, 353, 611, 754, 1155 |
| GluI | GCNGC | 9 cut(s) | 25, 266, 302, 325, 435, 688, 930, 1032, 1638 |
| GsaI | CCCAGC | 1 cut(s) | 1316 |
| GsuI | CTGGAG | 1 cut(s) | 584 |
| HaeIII | GGCC | 8 cut(s) | 54, 117, 282, 344, 359, 710, 1232, 1421 |
| HapII | CCGG | 3 cut(s) | 59, 118, 467 |
| HgaI | GACGC | 2 cut(s) | 16, 185 |
| HhaI | GCGC | 5 cut(s) | 270, 354, 612, 755, 1156 |
| Hin1I | GRCGYC | 2 cut(s) | 27, 177 |
| Hin1II | CATG | 5 cut(s) | 421, 481, 751, 860, 1358 |
| Hin6I | GCGC | 5 cut(s) | 268, 352, 610, 753, 1154 |
| HinP1I | GCGC | 5 cut(s) | 268, 352, 610, 753, 1154 |
| HincII | GTYRAC | 2 cut(s) | 250, 1123 |
| HindII | GTYRAC | 2 cut(s) | 250, 1123 |
| HinfI | GANTC | 7 cut(s) | 203, 230, 591, 790, 1144, 1210, 1414 |
| HpaI | GTTAAC | 1 cut(s) | 1123 |
| HpaII | CCGG | 3 cut(s) | 59, 118, 467 |
| HphI | GGTGA | 5 cut(s) | 311, 514, 799, 979, 1504 |
| Hpy166II | GTNNAC | 5 cut(s) | 250, 415, 769, 1123, 1453 |
| Hpy188I | TCNGA | 8 cut(s) | 106, 175, 489, 670, 1107, 1149, 1215, 1413 |
| Hpy188III | TCNNGA | 4 cut(s) | 508, 631, 869, 1340 |
| Hpy8I | GTNNAC | 5 cut(s) | 250, 415, 769, 1123, 1453 |
| Hpy99I | CGWCG | 8 cut(s) | 47, 179, 203, 227, 230, 251, 254, 332 |
| HpyCH4III | ACNGT | 4 cut(s) | 47, 526, 583, 715 |
| HpyCH4IV | ACGT | 2 cut(s) | 78, 823 |
| HpyCH4V | TGCA | 5 cut(s) | 66, 444, 1013, 1091, 1622 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 310, 384, 576, 844, 1040, 1229, 1289 |
| HpyF3I | CTNAG | 5 cut(s) | 537, 867, 917, 1212, 1506 |
| HpySE526I | ACGT | 2 cut(s) | 78, 823 |
| Hsp92I | GRCGYC | 2 cut(s) | 27, 177 |
| Hsp92II | CATG | 5 cut(s) | 421, 481, 751, 860, 1358 |
| HspAI | GCGC | 5 cut(s) | 268, 352, 610, 753, 1154 |
| KspAI | GTTAAC | 1 cut(s) | 1123 |
| LmnI | GCTCC | 5 cut(s) | 107, 164, 318, 871, 997 |
| Lsp1109I | GCAGC | 4 cut(s) | 313, 916, 1018, 1624 |
| LweI | GCATC | 1 cut(s) | 1531 |
| MaeI | CTAG | 1 cut(s) | 759 |
| MaeII | ACGT | 2 cut(s) | 78, 823 |
| MaeIII | GTNAC | 5 cut(s) | 380, 652, 787, 985, 1049 |
| MbiI | CCGCTC | 3 cut(s) | 102, 159, 622 |
| MboII | GAAGA | 6 cut(s) | 175, 536, 541, 713, 1577, 1619 |
| MflI | RGATCY | 3 cut(s) | 489, 541, 1498 |
| MhlI | GDGCHC | 3 cut(s) | 56, 376, 562 |
| MlsI | TGGCCA | 1 cut(s) | 1232 |
| MluCI | AATT | 5 cut(s) | 275, 946, 1163, 1202, 1434 |
| MluNI | TGGCCA | 1 cut(s) | 1232 |
| MlyI | GAGTC | 5 cut(s) | 212, 224, 784, 1138, 1204 |
| MmeI | TCCRAC | 3 cut(s) | 198, 219, 778 |
| Mox20I | TGGCCA | 1 cut(s) | 1232 |
| MscI | TGGCCA | 1 cut(s) | 1232 |
| MseI | TTAA | 5 cut(s) | 939, 1122, 1242, 1407, 1678 |
| MslI | CAYNNNNRTG | 2 cut(s) | 785, 1338 |
| Msp20I | TGGCCA | 1 cut(s) | 1232 |
| MspCI | CTTAAG | 1 cut(s) | 1241 |
| MspI | CCGG | 3 cut(s) | 59, 118, 467 |
| MspR9I | CCNGG | 2 cut(s) | 286, 563 |
| Mva1269I | GAATGC | 1 cut(s) | 1156 |
| MvaI | CCWGG | 2 cut(s) | 286, 563 |
| MvnI | CGCG | 3 cut(s) | 268, 327, 354 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 310, 384, 576, 844, 1040, 1229, 1289 |
| NlaIII | CATG | 5 cut(s) | 421, 481, 751, 860, 1358 |
| NlaIV | GGNNCC | 9 cut(s) | 53, 54, 134, 283, 296, 559, 873, 1500, 1533 |
| NmeAIII | GCCGAG | 4 cut(s) | 93, 192, 385, 934 |
| NmuCI | GTSAC | 4 cut(s) | 380, 652, 787, 985 |
| NsbI | TGCGCA | 1 cut(s) | 754 |
| NspI | RCATGY | 2 cut(s) | 421, 481 |
| PaeR7I | CTCGAG | 2 cut(s) | 617, 792 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 183, 219, 225 |
| PctI | GAATGC | 1 cut(s) | 1156 |
| PfeI | GAWTC | 2 cut(s) | 591, 1414 |
| PflFI | GACNNNGTC | 1 cut(s) | 179 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1428 |
| PkrI | GCNGC | 9 cut(s) | 26, 267, 303, 326, 436, 689, 931, 1033, 1639 |
| PleI | GAGTC | 5 cut(s) | 211, 224, 784, 1138, 1204 |
| PpsI | GAGTC | 5 cut(s) | 211, 224, 784, 1138, 1204 |
| PshBI | ATTAAT | 1 cut(s) | 1407 |
| Psp6I | CCWGG | 2 cut(s) | 284, 561 |
| PspEI | GGTNACC | 1 cut(s) | 985 |
| PspFI | CCCAGC | 1 cut(s) | 1312 |
| PspGI | CCWGG | 2 cut(s) | 284, 561 |
| PspN4I | GGNNCC | 9 cut(s) | 53, 54, 134, 283, 296, 559, 873, 1500, 1533 |
| PspOMI | GGGCCC | 1 cut(s) | 52 |
| PspPI | GGNCC | 6 cut(s) | 4, 52, 53, 132, 281, 1272 |
| PspXI | VCTCGAGB | 2 cut(s) | 617, 792 |
| PsuI | RGATCY | 3 cut(s) | 489, 541, 1498 |
| PsyI | GACNNNGTC | 1 cut(s) | 179 |
| RsaI | GTAC | 1 cut(s) | 1488 |
| RsaNI | GTAC | 1 cut(s) | 1487 |
| RseI | CAYNNNNRTG | 2 cut(s) | 785, 1338 |
| SalI | GTCGAC | 1 cut(s) | 248 |
| SaqAI | TTAA | 5 cut(s) | 939, 1122, 1242, 1407, 1678 |
| SatI | GCNGC | 9 cut(s) | 25, 266, 302, 325, 435, 688, 930, 1032, 1638 |
| Sau96I | GGNCC | 6 cut(s) | 4, 52, 53, 132, 281, 1272 |
| SchI | GAGTC | 5 cut(s) | 212, 224, 784, 1138, 1204 |
| ScrFI | CCNGG | 2 cut(s) | 286, 563 |
| SduI | GDGCHC | 3 cut(s) | 56, 376, 562 |
| SfaNI | GCATC | 1 cut(s) | 1531 |
| Sfr274I | CTCGAG | 2 cut(s) | 617, 792 |
| SgrDI | CGTCGACG | 1 cut(s) | 248 |
| SinI | GGWCC | 3 cut(s) | 4, 132, 1272 |
| SlaI | CTCGAG | 2 cut(s) | 617, 792 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 785, 1338 |
| SmlI | CTYRAG | 3 cut(s) | 617, 792, 1241 |
| SmoI | CTYRAG | 3 cut(s) | 617, 792, 1241 |
| Sse9I | AATT | 5 cut(s) | 275, 946, 1163, 1202, 1434 |
| SspI | AATATT | 1 cut(s) | 1178 |
| SspMI | CTAG | 1 cut(s) | 759 |
| StyD4I | CCNGG | 2 cut(s) | 284, 561 |
| StyI | CCWWGG | 6 cut(s) | 7, 648, 758, 888, 990, 1386 |
| TaaI | ACNGT | 4 cut(s) | 47, 526, 583, 715 |
| TaiI | ACGT | 2 cut(s) | 81, 826 |
| TaqI | TCGA | 9 cut(s) | 15, 186, 249, 398, 509, 589, 618, 793, 924 |
| TasI | AATT | 5 cut(s) | 275, 946, 1163, 1202, 1434 |
| TatI | WGTACW | 1 cut(s) | 1486 |
| TauI | GCSGC | 5 cut(s) | 27, 268, 327, 437, 690 |
| TfiI | GAWTC | 2 cut(s) | 591, 1414 |
| Tru1I | TTAA | 5 cut(s) | 939, 1122, 1242, 1407, 1678 |
| Tru9I | TTAA | 5 cut(s) | 939, 1122, 1242, 1407, 1678 |
| TscAI | CASTG | 3 cut(s) | 718, 882, 921 |
| TseFI | GTSAC | 4 cut(s) | 380, 652, 787, 985 |
| TseI | GCWGC | 4 cut(s) | 301, 929, 1031, 1637 |
| Tsp45I | GTSAC | 4 cut(s) | 380, 652, 787, 985 |
| TspDTI | ATGAA | 6 cut(s) | 714, 1032, 1176, 1322, 1322, 1478 |
| TspRI | CASTG | 3 cut(s) | 718, 882, 921 |
| Tth111I | GACNNNGTC | 1 cut(s) | 179 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1428 |
| Vha464I | CTTAAG | 1 cut(s) | 1241 |
| VpaK11BI | GGWCC | 3 cut(s) | 4, 132, 1272 |
| VspI | ATTAAT | 1 cut(s) | 1407 |
| XapI | RAATTY | 3 cut(s) | 275, 946, 1163 |
| XceI | RCATGY | 2 cut(s) | 421, 481 |
| XhoI | CTCGAG | 2 cut(s) | 617, 792 |
| XmaJI | CCTAGG | 1 cut(s) | 758 |
| XmiI | GTMKAC | 3 cut(s) | 249, 414, 768 |
| XspI | CTAG | 1 cut(s) | 759 |