MD05G1338100.v1.1

mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In the eIF-3 complex, eif3d specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010260
Physical Location & Seq
Reverse (-)
45956939 .. 45959683
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1338100.v1.1.491

Sequence Viewer

Length: 1701 bp
ATGGTCGAAGGTTTCGAAGTCGGCGGCGTACCTTTCAACCCCGACGGGTGGGGCCCACCGGACAGCACCACCACCCCAACCACCTCTTCCAATTTGCCCCAAAACGTCCCCTTCGCCCCCTTCTCCCGCTCTGAGAAGCTTGGCCGGATCGCCGACTGGACCCGCACCTTCAACAATCCTGGCCGCTCCAAGAACCCATCTGACGCCGTCTTCGACTTCTCCAACGACGAGTCCTTCCCCGCCTCTGCCGATGACGACGCTTCGTTCCGTCTCGTCGATGGAAAGCCCCCTCCGCGCCCCAGATTCGGCCCCAAGTGGCGGTTCCAGCAGCAGCGACAGCTCCCCCACCGCCGCGACGAGGAGGTCGAGGCCAAGAAGCGCGAGGCTGAGAAGGAGCGGGCCCGCCGCGACCGCCAGTACAACATGAACCGGTCCAACGTCAACGCCCCTCGCCGAGAAGCCGCGGTTTTCAAATCTTCCGTCGATATCCAGCCGGAGTGGAACATGCTGGAACAGATCCCCTTCTCCACATTCTCTAAGCTCTCGTTTTCTGTACCGGAACCCGAGGACCTCCTCCTCTGCGGCGGACTCGAGTTCTACGATCGCTCCGTCGATCGAATCACCCCCAAGAACGAGAGGCGGCTCGAGAGGTTCAAGAACAGGAACTTCTTCAAGGTCACCACCACCGATGACCCCGTCATCCGCCGCCTCGCAAACGAGGATAAAGCCACAGTCTTTGCCACTGATACGATTCTCTCGACTCTCATGTGTGCACCCAGGTCAGTTTACTCTTGGGATATTGTGGTGCAGAAAGTTGGGAACAAATTGTTCTTTGATAAACGTGATGGGTCGCAATTGGATTTGCTTTCGGTCCACGAAACCTCTCAGGAGCCCTTGCCTGAAGCTAAGGATGATATCAATTCAGCTTACTCGTTGAGTGTCGAGGCTGCTTATATCAATCAGAACTTTTCACAGCAGGTTTTGGTTAGGGATGGAAATAAGGTCACGTTCGAGGAGCCTAACCCCTTTGCGAATGAAGGAGAGGAGGTTGCTTCCGTTGCTTATCGCTACCGGAGGTGGAAGCTTGAGGAGGGAATGTATCTGGTTGCGAGGTGTGAGGTGCAGAGTGTTATGGAGGTGAATAACCAGAGGTCGTTTTTGACCCTGAATGCGCTTAATGAGTTTGATCCGAAGTATTCGGGTGTCGATTGGAGGCAGAAGTTGGAGACTCAGAGGGGAGCTGTATTGGCAACTGAGCTGAAGAACAATGCTAACAAGTTGGCAAAATGGACTGCACAAGCGCTCCTGGCCAGTGCTGATTTGATGAAATTGGGTTACGTTTCAAGGGTTCATCCTCGTGATCACTTTAACCATGTGATTTTGGCTGTGGTTGGATACAAGCCCAAGGAGTTTGCCTCGCAGATTAATCTGAACCAAAACAGTATGTGGGGGATTGTGAAGTCGATTGTGGACTTGTGTATGAAATTGAATGAGGGCAAGTATGTGCTTGTTAAGGACCCATCGAAGCCCCAAGTTAGGATATATGAGGTGCCTCCAGATGCTTTTGAGAATGACTATGTGGAGGAGCCACTGCCCGAGGATGAGCAAGTTCAGCCACCGACGGAGGAAGGGCAAGATGCTGAGCCGAATGTTGCTGCTAATGATGTTGAGGATAAGCCGGTTGATGCTGTTGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005654 GO:0005737 GO:0005783 GO:0005789 GO:0005829 GO:0005852 GO:0005911 GO:0006412 GO:0006413 GO:0006464 GO:0006470 GO:0006518 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006913 GO:0007154 GO:0007165 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009639 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010017 GO:0010019 GO:0010035 GO:0010038 GO:0010380 GO:0010467 GO:0012505 GO:0016020 GO:0016021 GO:0016311 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0023052 GO:0030054 GO:0030176 GO:0031224 GO:0031227 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0042175 GO:0042221 GO:0042578 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046686 GO:0046906 GO:0046907 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051171 GO:0051172 GO:0051179 GO:0051193 GO:0051195 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071704 GO:0090056 GO:0097159 GO:0098827 GO:0104004 GO:0140096 GO:1901363 GO:1901401 GO:1901402 GO:1901463 GO:1901464 GO:1901564 GO:1901566 GO:1901576 GO:1902325
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-3_zeta

Protein Analysis

567

Amino Acids

64.48

Weight (kDa)

5.16

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-3_zeta PF05091 7 - 516 7.4e-197 Eukaryotic translation initiation factor 3 subunit 7 (eIF-3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20980 AT4G20980 AT4G20980 AT4G20980 AT5G44320
fragaria_vesca FvH4_3g37950 FvH4_5g27490
malus_domestica MD05G1338100.v1.1
prunus_persica Prupe.4G048700_v2.0.a1
pyrus_communis pycom10g24520
rosa_chinensis RchiOBHm_Chr5g0048541
rosa_laevigata RLG00000034531
rosa_multiflora Rmu_sc0007341.1_g000041
rosa_roxburghii Rroxscaffold_1G00033270
rosa_rugosa Rorug05G0239400
rosa_samantha Rh5AG319100 Rh5BG329500 Rh5CG355200 Rh5DG340700
rosa_wichuraiana Rw0G021230 Rw5G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 362
Acc36I ACCTGC 1 cut(s) 967
AccB1I GGYRCC 1 cut(s) 1549
AccBSI CCGCTC 3 cut(s) 129, 186, 397
AccII CGCG 5 cut(s) 295, 354, 381, 408, 464
AclWI GGATC 3 cut(s) 155, 511, 1181
AcoI YGGCCR 3 cut(s) 142, 181, 1308
AcuI CTGAAG 2 cut(s) 921, 1280
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 3 cut(s) 30, 419, 555
AfeI AGCGCT 1 cut(s) 1302
AfiI CCNNNNNNNGG 5 cut(s) 48, 305, 318, 358, 1536
AgeI ACCGGT 1 cut(s) 429
AgsI TTSAA 8 cut(s) 37, 172, 472, 655, 673, 1344, 1489, 1694
AjnI CCWGG 3 cut(s) 178, 776, 1305
AjuI GAANNNNNNNTTGG 4 cut(s) 70, 102, 305, 337
AluBI AGCT 9 cut(s) 139, 340, 541, 905, 926, 1084, 1241, 1258, 1697
AluI AGCT 9 cut(s) 139, 340, 541, 905, 926, 1084, 1241, 1258, 1697
Alw21I GWGCWC 1 cut(s) 775
Alw26I GTCTC 2 cut(s) 275, 1220
Alw44I GTGCAC 1 cut(s) 771
AlwI GGATC 3 cut(s) 155, 511, 1181
Ama87I CYCGRG 4 cut(s) 563, 590, 644, 1595
Aor51HI AGCGCT 1 cut(s) 1302
AoxI GGCC 7 cut(s) 52, 142, 181, 307, 369, 399, 1308
ApaI GGGCCC 2 cut(s) 56, 403
ApaLI GTGCAC 1 cut(s) 771
ApeKI GCWGC 4 cut(s) 328, 331, 947, 1655
ArsI GACNNNNNNTTYG 4 cut(s) 194, 226, 465, 497
AseI ATTAAT 1 cut(s) 1425
AsiGI ACCGGT 1 cut(s) 429
Asp700I GAANNNNTTC 1 cut(s) 668
AspLEI GCGC 4 cut(s) 297, 381, 1174, 1303
AsuHPI GGTGA 3 cut(s) 613, 670, 1150
AsuII TTCGAA 1 cut(s) 15
AvaI CYCGRG 4 cut(s) 563, 590, 644, 1595
AvaII GGWCC 5 cut(s) 159, 432, 568, 871, 1516
BaeGI GKGCMC 3 cut(s) 56, 403, 775
BalI TGGCCA 1 cut(s) 1310
BanI GGYRCC 1 cut(s) 1549
BanII GRGCYC 3 cut(s) 56, 403, 894
BauI CACGAG 1 cut(s) 1356
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 775
BbvI GCAGC 4 cut(s) 340, 343, 934, 1642
BccI CCATC 5 cut(s) 205, 272, 839, 986, 1528
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 3 cut(s) 180, 778, 1307
BciVI GTATCC 1 cut(s) 1388
BclI TGATCA 1 cut(s) 1360
BcoDI GTCTC 2 cut(s) 275, 1220
BfoI RGCGCY 1 cut(s) 1304
BfuAI ACCTGC 1 cut(s) 967
BfuI GTATCC 1 cut(s) 1388
BlpI GCTNAGC 1 cut(s) 1641
Bme1390I CCNGG 3 cut(s) 180, 778, 1307
Bme18I GGWCC 5 cut(s) 159, 432, 568, 871, 1516
BmeT110I CYCGRG 4 cut(s) 563, 590, 644, 1595
BmrFI CCNGG 3 cut(s) 180, 778, 1307
BmsI GCATC 3 cut(s) 1549, 1627, 1675
BpiI GAAGAC 1 cut(s) 202
BplI GAGNNNNNCTC 2 cut(s) 1400, 1432
BpmI CTGGAG 1 cut(s) 1539
Bpu10I CCTNAGC 1 cut(s) 906
Bpu1102I GCTNAGC 1 cut(s) 1641
Bpu14I TTCGAA 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 1106
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 5 cut(s) 462, 564, 776, 1404, 1596
BsaWI WCCGGW 4 cut(s) 58, 429, 556, 1071
BsaXI ACNNNNNCTCC 8 cut(s) 590, 620, 1032, 1062, 1082, 1112, 1287, 1317
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 305, 318, 358, 1536
Bse118I RCCGGY 2 cut(s) 429, 1678
Bse1I ACTGG 3 cut(s) 161, 415, 1311
BseBI CCWGG 3 cut(s) 180, 778, 1307
BseDI CCNNGG 5 cut(s) 462, 564, 776, 1404, 1596
BseGI GGATG 5 cut(s) 699, 916, 997, 1351, 1606
BseLI CCNNNNNNNGG 5 cut(s) 48, 305, 318, 358, 1536
BseMII CTCAG 6 cut(s) 123, 378, 899, 1244, 1245, 1632
BseNI ACTGG 3 cut(s) 161, 415, 1311
BseRI GAGGAG 7 cut(s) 374, 563, 566, 1028, 1058, 1103, 1598
BseSI GKGCMC 3 cut(s) 56, 403, 775
BseXI GCAGC 4 cut(s) 340, 343, 934, 1642
BsgI GTGCAG 3 cut(s) 827, 1142, 1278
Bsh1236I CGCG 5 cut(s) 295, 354, 381, 408, 464
Bsh1285I CGRYCG 3 cut(s) 412, 604, 616
BshFI GGCC 7 cut(s) 54, 144, 183, 309, 371, 401, 1310
BshNI GGYRCC 1 cut(s) 1549
BshTI ACCGGT 1 cut(s) 429
BsiEI CGRYCG 3 cut(s) 412, 604, 616
BsiHKAI GWGCWC 1 cut(s) 775
BsiHKCI CYCGRG 4 cut(s) 563, 590, 644, 1595
BsiSI CCGG 7 cut(s) 59, 145, 430, 494, 557, 1072, 1679
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 5 cut(s) 48, 305, 318, 358, 1536
BsmAI GTCTC 2 cut(s) 275, 1220
BsmBI CGTCTC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 92
BsmI GAATGC 1 cut(s) 1174
BsnI GGCC 7 cut(s) 54, 144, 183, 309, 371, 401, 1310
BsoBI CYCGRG 4 cut(s) 563, 590, 644, 1595
Bsp119I TTCGAA 1 cut(s) 15
Bsp120I GGGCCC 2 cut(s) 52, 399
Bsp1286I GDGCHC 4 cut(s) 56, 403, 775, 894
Bsp143I GATC 6 cut(s) 147, 516, 601, 613, 1186, 1360
Bsp1720I GCTNAGC 1 cut(s) 1641
BspANI GGCC 7 cut(s) 54, 144, 183, 309, 371, 401, 1310
BspCNI CTCAG 6 cut(s) 124, 379, 898, 1243, 1246, 1633
BspFNI CGCG 5 cut(s) 295, 354, 381, 408, 464
BspMI ACCTGC 1 cut(s) 967
BspPI GGATC 3 cut(s) 155, 511, 1181
BspT104I TTCGAA 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 1549
BsrBI CCGCTC 3 cut(s) 129, 186, 397
BsrFI RCCGGY 2 cut(s) 429, 1678
BsrI ACTGG 3 cut(s) 161, 415, 1311
BssAI RCCGGY 2 cut(s) 429, 1678
BssECI CCNNGG 5 cut(s) 462, 564, 776, 1404, 1596
BssMI GATC 6 cut(s) 147, 516, 601, 613, 1186, 1360
BssNI GRCGYC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 1356
BssT1I CCWWGG 1 cut(s) 1404
Bst2BI CACGAG 1 cut(s) 1356
Bst2UI CCWGG 3 cut(s) 180, 778, 1307
Bst4CI ACNGT 2 cut(s) 733, 1442
Bst6I CTCTTC 1 cut(s) 91
BstACI GRCGYC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 399, 403
BstDEI CTNAG 8 cut(s) 132, 387, 537, 885, 906, 1230, 1254, 1641
BstDSI CCRYGG 1 cut(s) 462
BstEII GGTNACC 1 cut(s) 676
BstF5I GGATG 5 cut(s) 699, 916, 997, 1351, 1606
BstFNI CGCG 5 cut(s) 295, 354, 381, 408, 464
BstH2I RGCGCY 1 cut(s) 1304
BstHHI GCGC 4 cut(s) 297, 381, 1174, 1303
BstKTI GATC 6 cut(s) 150, 519, 604, 616, 1189, 1363
BstMAI GTCTC 2 cut(s) 275, 1220
BstMBI GATC 6 cut(s) 147, 516, 601, 613, 1186, 1360
BstMCI CGRYCG 3 cut(s) 412, 604, 616
BstMWI GCNNNNNNNGC 9 cut(s) 292, 325, 337, 411, 1058, 1247, 1307, 1612, 1694
BstNI CCWGG 3 cut(s) 180, 778, 1307
BstNSI RCATGY 1 cut(s) 508
BstPI GGTNACC 1 cut(s) 676
BstSCI CCNGG 3 cut(s) 178, 776, 1305
BstSLI GKGCMC 3 cut(s) 56, 403, 775
BstUI CGCG 5 cut(s) 295, 354, 381, 408, 464
BstV1I GCAGC 4 cut(s) 340, 343, 934, 1642
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 1 cut(s) 516
BstYI RGATCY 1 cut(s) 516
BsuI GTATCC 1 cut(s) 1388
BsuRI GGCC 7 cut(s) 54, 144, 183, 309, 371, 401, 1310
BtgI CCRYGG 1 cut(s) 462
BtsCI GGATG 5 cut(s) 699, 916, 997, 1351, 1606
BtsI GCAGTG 1 cut(s) 1589
BtsIMutI CAGTG 3 cut(s) 741, 1318, 1589
BveI ACCTGC 1 cut(s) 967
Cac8I GCNNGC 2 cut(s) 399, 403
CfoI GCGC 4 cut(s) 297, 381, 1174, 1303
Cfr10I RCCGGY 2 cut(s) 429, 1678
Cfr42I CCGCGG 1 cut(s) 465
CseI GACGC 2 cut(s) 212, 266
Csp6I GTAC 3 cut(s) 29, 418, 554
CspAI ACCGGT 1 cut(s) 429
CspCI CAANNNNNGTGG 2 cut(s) 718, 753
CviAII CATG 4 cut(s) 424, 505, 766, 1373
CviQI GTAC 3 cut(s) 29, 418, 554
DdeI CTNAG 8 cut(s) 132, 387, 537, 885, 906, 1230, 1254, 1641
DpnI GATC 6 cut(s) 149, 518, 603, 615, 1188, 1362
DpnII GATC 6 cut(s) 147, 516, 601, 613, 1186, 1360
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 3 cut(s) 142, 181, 1308
Eam1104I CTCTTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 91
EciI GGCGGA 2 cut(s) 600, 692
Eco130I CCWWGG 1 cut(s) 1404
Eco24I GRGCYC 3 cut(s) 56, 403, 894
Eco32I GATATC 2 cut(s) 487, 916
Eco47I GGWCC 5 cut(s) 159, 432, 568, 871, 1516
Eco47III AGCGCT 1 cut(s) 1302
Eco57I CTGAAG 2 cut(s) 921, 1280
Eco88I CYCGRG 4 cut(s) 563, 590, 644, 1595
Eco91I GGTNACC 1 cut(s) 676
EcoO109I RGGNCCY 3 cut(s) 52, 568, 1516
EcoO65I GGTNACC 1 cut(s) 676
EcoRII CCWGG 3 cut(s) 178, 776, 1305
EcoRV GATATC 2 cut(s) 487, 916
EcoT14I CCWWGG 1 cut(s) 1404
EcoT38I GRGCYC 3 cut(s) 56, 403, 894
ErhI CCWWGG 1 cut(s) 1404
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 4 cut(s) 427, 508, 769, 1376
FaqI GGGAC 1 cut(s) 92
FatI CATG 4 cut(s) 423, 504, 765, 1372
FauI CCCGC 5 cut(s) 134, 170, 247, 390, 410
FbaI TGATCA 1 cut(s) 1360
FokI GGATG 5 cut(s) 686, 923, 1004, 1338, 1613
FriOI GRGCYC 3 cut(s) 56, 403, 894
GlaI GCGC 4 cut(s) 296, 380, 1173, 1302
GsuI CTGGAG 1 cut(s) 1539
HaeII RGCGCY 1 cut(s) 1304
HaeIII GGCC 7 cut(s) 54, 144, 183, 309, 371, 401, 1310
HapII CCGG 7 cut(s) 59, 145, 430, 494, 557, 1072, 1679
HgaI GACGC 2 cut(s) 212, 266
HhaI GCGC 4 cut(s) 297, 381, 1174, 1303
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 4 cut(s) 427, 508, 769, 1376
Hin6I GCGC 4 cut(s) 295, 379, 1172, 1301
HinP1I GCGC 4 cut(s) 295, 379, 1172, 1301
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HindIII AAGCTT 3 cut(s) 137, 1082, 1695
HinfI GANTC 7 cut(s) 230, 303, 588, 618, 751, 760, 1228
HpaII CCGG 7 cut(s) 59, 145, 430, 494, 557, 1072, 1679
HphI GGTGA 3 cut(s) 613, 670, 1150
Hpy166II GTNNAC 5 cut(s) 442, 773, 787, 874, 1471
Hpy188I TCNGA 6 cut(s) 133, 202, 963, 1191, 1233, 1431
Hpy188III TCNNGA 6 cut(s) 646, 655, 757, 887, 1358, 1556
Hpy8I GTNNAC 5 cut(s) 442, 773, 787, 874, 1471
Hpy99I CGWCG 8 cut(s) 47, 230, 260, 278, 359, 485, 614, 1624
HpyAV CCTTC 8 cut(s) 121, 130, 178, 244, 385, 532, 1031, 1622
HpyCH4III ACNGT 2 cut(s) 733, 1442
HpyCH4IV ACGT 5 cut(s) 105, 438, 841, 1007, 1338
HpyCH4V TGCA 4 cut(s) 773, 808, 1123, 1295
HpyF10VI GCNNNNNNNGC 9 cut(s) 292, 325, 337, 411, 1058, 1247, 1307, 1612, 1694
HpyF3I CTNAG 8 cut(s) 132, 387, 537, 885, 906, 1230, 1254, 1641
HpySE526I ACGT 5 cut(s) 105, 438, 841, 1007, 1338
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 4 cut(s) 427, 508, 769, 1376
HspAI GCGC 4 cut(s) 295, 379, 1172, 1301
Ksp22I TGATCA 1 cut(s) 1360
KspI CCGCGG 1 cut(s) 465
Kzo9I GATC 6 cut(s) 147, 516, 601, 613, 1186, 1360
LmnI GCTCC 9 cut(s) 191, 345, 394, 611, 889, 1015, 1238, 1308, 1585
Lsp1109I GCAGC 4 cut(s) 340, 343, 934, 1642
LweI GCATC 3 cut(s) 1549, 1627, 1675
MaeII ACGT 5 cut(s) 105, 438, 841, 1007, 1338
MaeIII GTNAC 3 cut(s) 676, 1003, 1334
MalI GATC 6 cut(s) 149, 518, 603, 615, 1188, 1362
MbiI CCGCTC 3 cut(s) 129, 186, 397
MboI GATC 6 cut(s) 147, 516, 601, 613, 1186, 1360
MboII GAAGA 5 cut(s) 78, 202, 468, 661, 1273
MfeI CAATTG 1 cut(s) 854
MflI RGATCY 1 cut(s) 516
MhlI GDGCHC 4 cut(s) 56, 403, 775, 894
MlsI TGGCCA 1 cut(s) 1310
MluCI AATT 6 cut(s) 91, 824, 854, 919, 1328, 1484
MluNI TGGCCA 1 cut(s) 1310
MlyI GAGTC 4 cut(s) 239, 582, 754, 1222
MmeI TCCRAC 4 cut(s) 246, 459, 1203, 1372
Mox20I TGGCCA 1 cut(s) 1310
MroXI GAANNNNTTC 1 cut(s) 668
MscI TGGCCA 1 cut(s) 1310
MseI TTAA 4 cut(s) 1176, 1368, 1425, 1512
MslI CAYNNNNRTG 1 cut(s) 1356
Msp20I TGGCCA 1 cut(s) 1310
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 7 cut(s) 59, 145, 430, 494, 557, 1072, 1679
MspR9I CCNGG 3 cut(s) 180, 778, 1307
MunI CAATTG 1 cut(s) 854
Mva1269I GAATGC 1 cut(s) 1174
MvaI CCWGG 3 cut(s) 180, 778, 1307
MvnI CGCG 5 cut(s) 295, 354, 381, 408, 464
MwoI GCNNNNNNNGC 9 cut(s) 292, 325, 337, 411, 1058, 1247, 1307, 1612, 1694
NdeII GATC 6 cut(s) 147, 516, 601, 613, 1186, 1360
NlaIII CATG 4 cut(s) 427, 508, 769, 1376
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 2 cut(s) 676, 1003
NspI RCATGY 1 cut(s) 508
NspV TTCGAA 1 cut(s) 15
PaeR7I CTCGAG 2 cut(s) 590, 644
PcsI WCGNNNNNNNCGW 6 cut(s) 12, 210, 363, 597, 606, 755
PctI GAATGC 1 cut(s) 1174
PdmI GAANNNNTTC 1 cut(s) 668
PfeI GAWTC 3 cut(s) 303, 618, 751
PflFI GACNNNGTC 2 cut(s) 206, 695
PinAI ACCGGT 1 cut(s) 429
Ple19I CGATCG 2 cut(s) 604, 616
PleI GAGTC 4 cut(s) 238, 582, 754, 1222
PpsI GAGTC 4 cut(s) 238, 582, 754, 1222
PpuMI RGGWCCY 2 cut(s) 568, 1516
PshBI ATTAAT 1 cut(s) 1425
Psp5II RGGWCCY 2 cut(s) 568, 1516
Psp6I CCWGG 3 cut(s) 178, 776, 1305
PspEI GGTNACC 1 cut(s) 676
PspGI CCWGG 3 cut(s) 178, 776, 1305
PspOMI GGGCCC 2 cut(s) 52, 399
PspPPI RGGWCCY 2 cut(s) 568, 1516
PspXI VCTCGAGB 1 cut(s) 590
PsuI RGATCY 1 cut(s) 516
PsyI GACNNNGTC 2 cut(s) 206, 695
PvuI CGATCG 2 cut(s) 604, 616
RsaI GTAC 3 cut(s) 30, 419, 555
RsaNI GTAC 3 cut(s) 29, 418, 554
RseI CAYNNNNRTG 1 cut(s) 1356
SacII CCGCGG 1 cut(s) 465
SaqAI TTAA 4 cut(s) 1176, 1368, 1425, 1512
Sau3AI GATC 6 cut(s) 147, 516, 601, 613, 1186, 1360
SchI GAGTC 4 cut(s) 239, 582, 754, 1222
ScrFI CCNGG 3 cut(s) 180, 778, 1307
SduI GDGCHC 4 cut(s) 56, 403, 775, 894
SfaNI GCATC 3 cut(s) 1549, 1627, 1675
Sfr274I CTCGAG 2 cut(s) 590, 644
Sfr303I CCGCGG 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 15
SgrBI CCGCGG 1 cut(s) 465
SinI GGWCC 5 cut(s) 159, 432, 568, 871, 1516
SlaI CTCGAG 2 cut(s) 590, 644
SmiMI CAYNNNNRTG 1 cut(s) 1356
SmlI CTYRAG 3 cut(s) 590, 644, 1085
SmoI CTYRAG 3 cut(s) 590, 644, 1085
Sse9I AATT 6 cut(s) 91, 824, 854, 919, 1328, 1484
StyD4I CCNGG 3 cut(s) 178, 776, 1305
StyI CCWWGG 1 cut(s) 1404
TaaI ACNGT 2 cut(s) 733, 1442
TaiI ACGT 5 cut(s) 108, 441, 844, 1010, 1341
TaqII GACCGA 1 cut(s) 859
TasI AATT 6 cut(s) 91, 824, 854, 919, 1328, 1484
TatI WGTACW 1 cut(s) 417
TauI GCSGC 8 cut(s) 27, 186, 354, 408, 464, 585, 643, 708
TfiI GAWTC 3 cut(s) 303, 618, 751
Tru1I TTAA 4 cut(s) 1176, 1368, 1425, 1512
Tru9I TTAA 4 cut(s) 1176, 1368, 1425, 1512
TscAI CASTG 3 cut(s) 748, 1318, 1596
TseFI GTSAC 2 cut(s) 676, 1003
TseI GCWGC 4 cut(s) 328, 331, 947, 1655
Tsp45I GTSAC 2 cut(s) 676, 1003
TspDTI ATGAA 5 cut(s) 440, 1050, 1340, 1340, 1496
TspGWI ACGGA 5 cut(s) 257, 469, 598, 1045, 1637
TspRI CASTG 3 cut(s) 748, 1318, 1596
Tth111I GACNNNGTC 2 cut(s) 206, 695
VneI GTGCAC 1 cut(s) 771
VpaK11BI GGWCC 5 cut(s) 159, 432, 568, 871, 1516
VspI ATTAAT 1 cut(s) 1425
XceI RCATGY 1 cut(s) 508
XhoI CTCGAG 2 cut(s) 590, 644
XmnI GAANNNNTTC 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.