pycom10g24520

mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In the eIF-3 complex, eif3d specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010260
Physical Location & Seq
Reverse (-)
26360885 .. 26362574
1690 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g24520.2

Sequence Viewer

Length: 1644 bp
ATGGTCGAAGGTTTCGAAGTCGGCGGCGTCCCTTTCAACCCCGACGGGTGGGGCCCACCTGAGAGCACCACCACCTCCAATTTGCCCCAAAACGTCCCCTTCGCCCCCTTCTCCCGCTCCGAGAAGCTCGGCCGGATCGCCGACTGGACCCGCACCTTCAACAATCCAGCCCGCTCCAAGAACCCATCCGACGCCGTCTTCGACTTCTCCAACGGTGAGTCGTTCCCCGGCTCCGCCGATGACGACGCGTCGTTCCGTCTCGTCGATGGAAAGCCCCCGCCGCGCCCCAGATTCGGCCCCAAGTGGCGGTTCCAGCAGCAGCGACAGCTCCCCCACCGCCGCGACGAGGAGGTCGAGGCCAAGAAGCGCGAGGCCGAGAAGGAGCGAGCCCGCCGTGACCGCCAGTACAACATGAACCGGTCCAACGTCAATGCCCCGCGCCGAGAAGCTGCGGTTTTCAAATCTTCCGTCGATATCCAACCTGAGTGGAACATGCTCGAACAGATCCCCTTCTCCACATTCTCGAAGCTCTCGTTTTCGGTTCCGGAGCCGGAGGATCTCCTCTTCTGCGGCGGACTCGAGTTCTACGATCGCTCTGTCGATCGAATCACCCCCAAGAACGAGAGGCGGCTCGAGCGGTTCAAGAACAGGAACTTCTTCAAGGATAAAGCCACAGTCTTTGCCACAGACACCATTCTCTCGACTCTTATGTGCGCACCCAGGTCAGTTTACTCTTGGGATATTGTTGTTCAGAAAGTTGGGAACAAATTATTCTTTGATAAACGTGATGGGTCGCAATTGGATTTGCTTTCGGTTCACGAGACATCTCAGGAGCCATTGCCTGAAGCTAAGGATGATATCAATTCAGCTTATTCGTTGAGTGTCGAGGCTGCTTATATCAATCAGAACTTTTCACAGCAGGTTTTGGTTAGGGATGGGAATAAGGTCACATTCGATGAGCCTAACCCGTTTGCAAACGAAGGAGAGGAGGTTGCTGCCGTTGCTTATCGCTACCGAAGGTGGAAGCTTGAGGAGGGAATGTATCTGGTTGCGAGGTGTGAGGTGCAAAGTGTTATGGAGGTGAATAATCAGAGGTCGTTTTTGACCCTGAATGCGCTTAATGAGTTTGATCCCAAGTATTCGGGTGTCGATTGGAGGCAGAAATTGGAGACTCAGAGGGGTGCTGTATTGGCAACTGAGCTTAAGAACAATGCCAACAAGTTGGCTAAATGGACTGCCCAAGCCCTCTTGGCCAGTGCCGATTTGATGAAGTTGGGTTACGTTTCGAGGGTTCATCCTCGTGATCACTTTAACCATGTGATTTTGGCTGTGGTAGGATACAAGCCCAAGGAGTTTGCCTCGCAGATTAATCTCAACCAAAACAGTATGTGGGGGATTGTGAAGTCGATTGTGGACTTGTGTATGAAGTTAAATGAGGGCAAGTATGTGCTTGTTAAGGACCCGTCGAAGCCCCAAGTTAGAATCTATGAGGTGCCTCCAGATGCTTTTGAGAATGATTATGTGGAGGAACCACTGCCCGAGGATGAGCAAGTTCAGCCACCCACGGAGGAAGGCCAAGGTGCTGAGCCGAATGTTGCTGCTAATGATGTTGAGGATAAGCCGGCTGATGCTGTTGCCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005654 GO:0005737 GO:0005783 GO:0005789 GO:0005829 GO:0005852 GO:0005911 GO:0006412 GO:0006413 GO:0006464 GO:0006470 GO:0006518 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006913 GO:0007154 GO:0007165 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009639 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010017 GO:0010019 GO:0010035 GO:0010038 GO:0010380 GO:0010467 GO:0012505 GO:0016020 GO:0016021 GO:0016311 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0023052 GO:0030054 GO:0030176 GO:0031224 GO:0031227 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0042175 GO:0042221 GO:0042578 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046686 GO:0046906 GO:0046907 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051171 GO:0051172 GO:0051179 GO:0051193 GO:0051195 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071704 GO:0090056 GO:0097159 GO:0098827 GO:0104004 GO:0140096 GO:1901363 GO:1901401 GO:1901402 GO:1901463 GO:1901464 GO:1901564 GO:1901566 GO:1901576 GO:1902325
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-3_zeta

Protein Analysis

548

Amino Acids

62.23

Weight (kDa)

5.34

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20980 AT4G20980 AT4G20980 AT4G20980 AT5G44320
fragaria_vesca FvH4_3g37950 FvH4_5g27490
malus_domestica MD05G1338100.v1.1
prunus_persica Prupe.4G048700_v2.0.a1
pyrus_communis pycom10g24520
rosa_chinensis RchiOBHm_Chr5g0048541
rosa_laevigata RLG00000034531
rosa_multiflora Rmu_sc0007341.1_g000041
rosa_roxburghii Rroxscaffold_1G00033270
rosa_rugosa Rorug05G0239400
rosa_samantha Rh5AG319100 Rh5BG329500 Rh5CG355200 Rh5DG340700
rosa_wichuraiana Rw0G021230 Rw5G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
Acc16I TGCGCA 1 cut(s) 715
Acc36I ACCTGC 1 cut(s) 910
AccB1I GGYRCC 1 cut(s) 1492
AccBSI CCGCTC 3 cut(s) 117, 174, 637
AccII CGCG 5 cut(s) 248, 283, 342, 369, 439
AccIII TCCGGA 1 cut(s) 544
AclWI GGATC 4 cut(s) 143, 499, 564, 1124
AcoI YGGCCR 2 cut(s) 130, 1251
AcuI CTGAAG 1 cut(s) 864
AcyI GRCGYC 2 cut(s) 27, 192
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 4 cut(s) 48, 293, 306, 346
AflII CTTAAG 1 cut(s) 1202
AflIII ACRYGT 1 cut(s) 246
AgeI ACCGGT 1 cut(s) 417
AgsI TTSAA 5 cut(s) 37, 160, 460, 643, 661
AhdI GACNNNNNGTC 1 cut(s) 247
AjnI CCWGG 1 cut(s) 719
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 8 cut(s) 127, 328, 449, 529, 848, 869, 1027, 1201
AluI AGCT 8 cut(s) 127, 328, 449, 529, 848, 869, 1027, 1201
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 3 cut(s) 263, 815, 1163
AlwI GGATC 4 cut(s) 143, 499, 564, 1124
Ama87I CYCGRG 3 cut(s) 578, 632, 1538
Aor13HI TCCGGA 1 cut(s) 544
AoxI GGCC 7 cut(s) 52, 130, 295, 357, 372, 1251, 1573
ApaI GGGCCC 1 cut(s) 56
ApeKI GCWGC 6 cut(s) 316, 319, 449, 890, 995, 1598
ArsI GACNNNNNNTTYG 4 cut(s) 182, 214, 453, 485
AseI ATTAAT 1 cut(s) 1368
AsiGI ACCGGT 1 cut(s) 417
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 5 cut(s) 285, 369, 441, 716, 1117
AspS9I GGNCC 6 cut(s) 52, 53, 147, 296, 420, 1459
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 3 cut(s) 227, 601, 1093
AsuII TTCGAA 1 cut(s) 15
AvaI CYCGRG 3 cut(s) 578, 632, 1538
AvaII GGWCC 3 cut(s) 147, 420, 1459
BaeGI GKGCMC 1 cut(s) 56
BalI TGGCCA 1 cut(s) 1253
BanI GGYRCC 1 cut(s) 1492
BanII GRGCYC 2 cut(s) 56, 391
BarI GAAGNNNNNNTAC 2 cut(s) 1262, 1294
BauI CACGAG 2 cut(s) 818, 1299
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 68
BbvI GCAGC 6 cut(s) 328, 331, 436, 877, 982, 1585
BccI CCATC 4 cut(s) 193, 260, 782, 929
BceAI ACGGC 3 cut(s) 179, 378, 983
BciT130I CCWGG 1 cut(s) 721
BciVI GTATCC 1 cut(s) 1331
BclI TGATCA 1 cut(s) 1303
BcnI CCSGG 1 cut(s) 228
BcoDI GTCTC 3 cut(s) 263, 815, 1163
BfrI CTTAAG 1 cut(s) 1202
BfuAI ACCTGC 1 cut(s) 910
BfuI GTATCC 1 cut(s) 1331
BglI GCCNNNNNGGC 1 cut(s) 1250
BlpI GCTNAGC 1 cut(s) 1584
Bme1390I CCNGG 2 cut(s) 228, 721
Bme18I GGWCC 3 cut(s) 147, 420, 1459
BmeRI GACNNNNNGTC 1 cut(s) 247
BmeT110I CYCGRG 3 cut(s) 578, 632, 1538
BmgT120I GGNCC 6 cut(s) 52, 53, 147, 296, 420, 1459
BmrFI CCNGG 2 cut(s) 228, 721
BmsI GCATC 2 cut(s) 1492, 1618
BpiI GAAGAC 1 cut(s) 190
BplI GAGNNNNNCTC 2 cut(s) 1343, 1375
BpmI CTGGAG 1 cut(s) 1482
Bpu10I CCTNAGC 1 cut(s) 849
Bpu1102I GCTNAGC 1 cut(s) 1584
Bpu14I TTCGAA 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 1049
BpuMI CCSGG 1 cut(s) 228
BsaHI GRCGYC 2 cut(s) 27, 192
BsaJI CCNNGG 6 cut(s) 226, 719, 1347, 1539, 1563, 1576
BsaWI WCCGGW 2 cut(s) 417, 544
BsaXI ACNNNNNCTCC 4 cut(s) 975, 1005, 1025, 1055
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 293, 306, 346
Bse118I RCCGGY 2 cut(s) 417, 1621
Bse1I ACTGG 3 cut(s) 149, 403, 1254
Bse3DI GCAATG 1 cut(s) 836
BseAI TCCGGA 1 cut(s) 544
BseBI CCWGG 1 cut(s) 721
BseDI CCNNGG 6 cut(s) 226, 719, 1347, 1539, 1563, 1576
BseGI GGATG 5 cut(s) 185, 859, 940, 1294, 1549
BseLI CCNNNNNNNGG 4 cut(s) 48, 293, 306, 346
BseMI GCAATG 1 cut(s) 836
BseMII CTCAG 6 cut(s) 51, 474, 842, 1187, 1188, 1575
BseNI ACTGG 3 cut(s) 149, 403, 1254
BseRI GAGGAG 4 cut(s) 362, 551, 1001, 1046
BseSI GKGCMC 1 cut(s) 56
BseX3I CGGCCG 1 cut(s) 130
BseXI GCAGC 6 cut(s) 328, 331, 436, 877, 982, 1585
Bsh1236I CGCG 5 cut(s) 248, 283, 342, 369, 439
Bsh1285I CGRYCG 3 cut(s) 133, 592, 604
BshFI GGCC 7 cut(s) 54, 132, 297, 359, 374, 1253, 1575
BshNI GGYRCC 1 cut(s) 1492
BshTI ACCGGT 1 cut(s) 417
BsiEI CGRYCG 3 cut(s) 133, 592, 604
BsiHKAI GWGCWC 1 cut(s) 68
BsiHKCI CYCGRG 3 cut(s) 578, 632, 1538
BsiSI CCGG 6 cut(s) 133, 228, 418, 545, 551, 1622
BslFI GGGAC 2 cut(s) 14, 80
BslI CCNNNNNNNGG 4 cut(s) 48, 293, 306, 346
BsmAI GTCTC 3 cut(s) 263, 815, 1163
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 14, 80
BsmI GAATGC 1 cut(s) 1117
BsnI GGCC 7 cut(s) 54, 132, 297, 359, 374, 1253, 1575
BsoBI CYCGRG 3 cut(s) 578, 632, 1538
Bsp119I TTCGAA 1 cut(s) 15
Bsp120I GGGCCC 1 cut(s) 52
Bsp1286I GDGCHC 3 cut(s) 56, 68, 391
Bsp13I TCCGGA 1 cut(s) 544
Bsp143I GATC 7 cut(s) 135, 504, 556, 589, 601, 1129, 1303
Bsp1720I GCTNAGC 1 cut(s) 1584
BspANI GGCC 7 cut(s) 54, 132, 297, 359, 374, 1253, 1575
BspCNI CTCAG 6 cut(s) 52, 475, 841, 1186, 1189, 1576
BspEI TCCGGA 1 cut(s) 544
BspFNI CGCG 5 cut(s) 248, 283, 342, 369, 439
BspMI ACCTGC 1 cut(s) 910
BspPI GGATC 4 cut(s) 143, 499, 564, 1124
BspT104I TTCGAA 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 1492
BspTI CTTAAG 1 cut(s) 1202
BsrBI CCGCTC 3 cut(s) 117, 174, 637
BsrDI GCAATG 1 cut(s) 836
BsrFI RCCGGY 2 cut(s) 417, 1621
BsrI ACTGG 3 cut(s) 149, 403, 1254
BssAI RCCGGY 2 cut(s) 417, 1621
BssECI CCNNGG 6 cut(s) 226, 719, 1347, 1539, 1563, 1576
BssMI GATC 7 cut(s) 135, 504, 556, 589, 601, 1129, 1303
BssNI GRCGYC 2 cut(s) 27, 192
BssSI CACGAG 2 cut(s) 818, 1299
BssT1I CCWWGG 2 cut(s) 1347, 1576
Bst2BI CACGAG 2 cut(s) 818, 1299
Bst2UI CCWGG 1 cut(s) 721
Bst4CI ACNGT 3 cut(s) 215, 676, 1385
Bst6I CTCTTC 1 cut(s) 569
BstACI GRCGYC 2 cut(s) 27, 192
BstAFI CTTAAG 1 cut(s) 1202
BstBI TTCGAA 1 cut(s) 15
BstC8I GCNNGC 4 cut(s) 172, 387, 391, 1623
BstDEI CTNAG 7 cut(s) 60, 483, 828, 849, 1173, 1197, 1584
BstDSI CCRYGG 1 cut(s) 1563
BstF5I GGATG 5 cut(s) 185, 859, 940, 1294, 1549
BstFNI CGCG 5 cut(s) 248, 283, 342, 369, 439
BstHHI GCGC 5 cut(s) 285, 369, 441, 716, 1117
BstKTI GATC 7 cut(s) 138, 507, 559, 592, 604, 1132, 1306
BstMAI GTCTC 3 cut(s) 263, 815, 1163
BstMBI GATC 7 cut(s) 135, 504, 556, 589, 601, 1129, 1303
BstMCI CGRYCG 3 cut(s) 133, 592, 604
BstNI CCWGG 1 cut(s) 721
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 2 cut(s) 226, 719
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 5 cut(s) 248, 283, 342, 369, 439
BstV1I GCAGC 6 cut(s) 328, 331, 436, 877, 982, 1585
BstV2I GAAGAC 1 cut(s) 190
BstX2I RGATCY 2 cut(s) 504, 556
BstXI CCANNNNNNTGG 1 cut(s) 1222
BstYI RGATCY 2 cut(s) 504, 556
BstZI CGGCCG 1 cut(s) 130
BsuI GTATCC 1 cut(s) 1331
BsuRI GGCC 7 cut(s) 54, 132, 297, 359, 374, 1253, 1575
BtgI CCRYGG 1 cut(s) 1563
BtsCI GGATG 5 cut(s) 185, 859, 940, 1294, 1549
BtsI GCAGTG 1 cut(s) 1532
BtsIMutI CAGTG 2 cut(s) 1261, 1532
BveI ACCTGC 1 cut(s) 910
Cac8I GCNNGC 4 cut(s) 172, 387, 391, 1623
CfoI GCGC 5 cut(s) 285, 369, 441, 716, 1117
Cfr10I RCCGGY 2 cut(s) 417, 1621
Cfr13I GGNCC 6 cut(s) 52, 53, 147, 296, 420, 1459
CseI GACGC 4 cut(s) 16, 200, 237, 254
Csp6I GTAC 1 cut(s) 406
CspAI ACCGGT 1 cut(s) 417
CspCI CAANNNNNGTGG 4 cut(s) 467, 502, 661, 696
CviAII CATG 3 cut(s) 412, 493, 1316
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 7 cut(s) 60, 483, 828, 849, 1173, 1197, 1584
DpnI GATC 7 cut(s) 137, 506, 558, 591, 603, 1131, 1305
DpnII GATC 7 cut(s) 135, 504, 556, 589, 601, 1129, 1303
DrdI GACNNNNNNGTC 1 cut(s) 350
DriI GACNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 2 cut(s) 130, 1251
EagI CGGCCG 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 569
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 569
EciI GGCGGA 2 cut(s) 223, 588
EclXI CGGCCG 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 1347, 1576
Eco24I GRGCYC 2 cut(s) 56, 391
Eco32I GATATC 2 cut(s) 475, 859
Eco47I GGWCC 3 cut(s) 147, 420, 1459
Eco52I CGGCCG 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 864
Eco88I CYCGRG 3 cut(s) 578, 632, 1538
EcoO109I RGGNCCY 2 cut(s) 52, 1459
EcoRII CCWGG 1 cut(s) 719
EcoRV GATATC 2 cut(s) 475, 859
EcoT14I CCWWGG 2 cut(s) 1347, 1576
EcoT38I GRGCYC 2 cut(s) 56, 391
ErhI CCWWGG 2 cut(s) 1347, 1576
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 3 cut(s) 415, 496, 1319
FaqI GGGAC 2 cut(s) 14, 80
FatI CATG 3 cut(s) 411, 492, 1315
FauI CCCGC 6 cut(s) 122, 158, 179, 285, 398, 444
FbaI TGATCA 1 cut(s) 1303
FokI GGATG 5 cut(s) 172, 866, 947, 1281, 1556
FriOI GRGCYC 2 cut(s) 56, 391
FspAI RTGCGCAY 1 cut(s) 715
FspI TGCGCA 1 cut(s) 715
GlaI GCGC 5 cut(s) 284, 368, 440, 715, 1116
GsuI CTGGAG 1 cut(s) 1482
HaeIII GGCC 7 cut(s) 54, 132, 297, 359, 374, 1253, 1575
HapII CCGG 6 cut(s) 133, 228, 418, 545, 551, 1622
HgaI GACGC 4 cut(s) 16, 200, 237, 254
HhaI GCGC 5 cut(s) 285, 369, 441, 716, 1117
Hin1I GRCGYC 2 cut(s) 27, 192
Hin1II CATG 3 cut(s) 415, 496, 1319
Hin6I GCGC 5 cut(s) 283, 367, 439, 714, 1115
HinP1I GCGC 5 cut(s) 283, 367, 439, 714, 1115
HindIII AAGCTT 1 cut(s) 1025
HinfI GANTC 7 cut(s) 218, 291, 576, 606, 703, 1171, 1482
HpaII CCGG 6 cut(s) 133, 228, 418, 545, 551, 1622
HphI GGTGA 3 cut(s) 227, 601, 1093
Hpy166II GTNNAC 3 cut(s) 730, 817, 1414
Hpy188I TCNGA 6 cut(s) 121, 190, 753, 906, 1092, 1176
Hpy188III TCNNGA 8 cut(s) 523, 545, 643, 700, 818, 830, 1301, 1499
Hpy8I GTNNAC 3 cut(s) 730, 817, 1414
Hpy99I CGWCG 8 cut(s) 47, 194, 248, 253, 266, 347, 473, 1468
HpyAV CCTTC 8 cut(s) 109, 118, 166, 373, 520, 974, 1011, 1565
HpyCH4III ACNGT 3 cut(s) 215, 676, 1385
HpyCH4IV ACGT 4 cut(s) 93, 426, 784, 1281
HpyCH4V TGCA 2 cut(s) 974, 1066
HpyF3I CTNAG 7 cut(s) 60, 483, 828, 849, 1173, 1197, 1584
HpySE526I ACGT 4 cut(s) 93, 426, 784, 1281
Hsp92I GRCGYC 2 cut(s) 27, 192
Hsp92II CATG 3 cut(s) 415, 496, 1319
HspAI GCGC 5 cut(s) 283, 367, 439, 714, 1115
Kpn2I TCCGGA 1 cut(s) 544
KroI GCCGGC 1 cut(s) 1621
KroNI GCCGGC 1 cut(s) 1623
Ksp22I TGATCA 1 cut(s) 1303
Kzo9I GATC 7 cut(s) 135, 504, 556, 589, 601, 1129, 1303
LmnI GCTCC 7 cut(s) 122, 179, 236, 333, 382, 547, 832
Lsp1109I GCAGC 6 cut(s) 328, 331, 436, 877, 982, 1585
LweI GCATC 2 cut(s) 1492, 1618
MaeII ACGT 4 cut(s) 93, 426, 784, 1281
MaeIII GTNAC 3 cut(s) 395, 946, 1277
MalI GATC 7 cut(s) 137, 506, 558, 591, 603, 1131, 1305
MbiI CCGCTC 3 cut(s) 117, 174, 637
MboI GATC 7 cut(s) 135, 504, 556, 589, 601, 1129, 1303
MboII GAAGA 4 cut(s) 190, 456, 556, 649
MfeI CAATTG 1 cut(s) 797
MflI RGATCY 2 cut(s) 504, 556
MhlI GDGCHC 3 cut(s) 56, 68, 391
MlsI TGGCCA 1 cut(s) 1253
MluCI AATT 5 cut(s) 79, 767, 797, 862, 1163
MluI ACGCGT 1 cut(s) 246
MluNI TGGCCA 1 cut(s) 1253
MlyI GAGTC 4 cut(s) 227, 570, 697, 1165
MmeI TCCRAC 4 cut(s) 213, 234, 447, 502
Mox20I TGGCCA 1 cut(s) 1253
MroI TCCGGA 1 cut(s) 544
MroNI GCCGGC 1 cut(s) 1621
MroXI GAANNNNTTC 1 cut(s) 656
MscI TGGCCA 1 cut(s) 1253
MseI TTAA 6 cut(s) 1119, 1203, 1311, 1368, 1430, 1455
MslI CAYNNNNRTG 1 cut(s) 1299
Msp20I TGGCCA 1 cut(s) 1253
MspCI CTTAAG 1 cut(s) 1202
MspI CCGG 6 cut(s) 133, 228, 418, 545, 551, 1622
MspR9I CCNGG 2 cut(s) 228, 721
MunI CAATTG 1 cut(s) 797
Mva1269I GAATGC 1 cut(s) 1117
MvaI CCWGG 1 cut(s) 721
MvnI CGCG 5 cut(s) 248, 283, 342, 369, 439
NaeI GCCGGC 1 cut(s) 1623
NciI CCSGG 1 cut(s) 228
NdeII GATC 7 cut(s) 135, 504, 556, 589, 601, 1129, 1303
NgoMIV GCCGGC 1 cut(s) 1621
NlaIII CATG 3 cut(s) 415, 496, 1319
NmeAIII GCCGAG 3 cut(s) 108, 400, 467
NmuCI GTSAC 2 cut(s) 395, 946
NsbI TGCGCA 1 cut(s) 715
NspI RCATGY 1 cut(s) 496
NspV TTCGAA 1 cut(s) 15
PaeR7I CTCGAG 2 cut(s) 578, 632
PcsI WCGNNNNNNNCGW 5 cut(s) 12, 198, 351, 530, 585
PctI GAATGC 1 cut(s) 1117
PdiI GCCGGC 1 cut(s) 1623
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 3 cut(s) 291, 606, 1482
PflFI GACNNNGTC 1 cut(s) 194
PinAI ACCGGT 1 cut(s) 417
Ple19I CGATCG 2 cut(s) 592, 604
PleI GAGTC 4 cut(s) 226, 570, 697, 1165
PpsI GAGTC 4 cut(s) 226, 570, 697, 1165
PpuMI RGGWCCY 1 cut(s) 1459
PshBI ATTAAT 1 cut(s) 1368
Psp5II RGGWCCY 1 cut(s) 1459
Psp6I CCWGG 1 cut(s) 719
PspGI CCWGG 1 cut(s) 719
PspOMI GGGCCC 1 cut(s) 52
PspPI GGNCC 6 cut(s) 52, 53, 147, 296, 420, 1459
PspPPI RGGWCCY 1 cut(s) 1459
PspXI VCTCGAGB 2 cut(s) 578, 632
PsuI RGATCY 2 cut(s) 504, 556
PsyI GACNNNGTC 1 cut(s) 194
PvuI CGATCG 2 cut(s) 592, 604
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
RseI CAYNNNNRTG 1 cut(s) 1299
SaqAI TTAA 6 cut(s) 1119, 1203, 1311, 1368, 1430, 1455
Sau3AI GATC 7 cut(s) 135, 504, 556, 589, 601, 1129, 1303
Sau96I GGNCC 6 cut(s) 52, 53, 147, 296, 420, 1459
SchI GAGTC 4 cut(s) 227, 570, 697, 1165
ScrFI CCNGG 2 cut(s) 228, 721
SduI GDGCHC 3 cut(s) 56, 68, 391
SfaNI GCATC 2 cut(s) 1492, 1618
Sfr274I CTCGAG 2 cut(s) 578, 632
SfuI TTCGAA 1 cut(s) 15
SinI GGWCC 3 cut(s) 147, 420, 1459
SlaI CTCGAG 2 cut(s) 578, 632
SmiMI CAYNNNNRTG 1 cut(s) 1299
SmlI CTYRAG 4 cut(s) 578, 632, 1028, 1202
SmoI CTYRAG 4 cut(s) 578, 632, 1028, 1202
Sse9I AATT 5 cut(s) 79, 767, 797, 862, 1163
StyD4I CCNGG 2 cut(s) 226, 719
StyI CCWWGG 2 cut(s) 1347, 1576
TaaI ACNGT 3 cut(s) 215, 676, 1385
TaiI ACGT 4 cut(s) 96, 429, 787, 1284
TasI AATT 5 cut(s) 79, 767, 797, 862, 1163
TatI WGTACW 1 cut(s) 405
TauI GCSGC 6 cut(s) 27, 283, 342, 573, 631, 1640
TfiI GAWTC 3 cut(s) 291, 606, 1482
Tru1I TTAA 6 cut(s) 1119, 1203, 1311, 1368, 1430, 1455
Tru9I TTAA 6 cut(s) 1119, 1203, 1311, 1368, 1430, 1455
TscAI CASTG 2 cut(s) 1261, 1539
TseFI GTSAC 2 cut(s) 395, 946
TseI GCWGC 6 cut(s) 316, 319, 449, 890, 995, 1598
Tsp45I GTSAC 2 cut(s) 395, 946
TspDTI ATGAA 4 cut(s) 428, 1283, 1283, 1439
TspGWI ACGGA 3 cut(s) 245, 457, 1580
TspRI CASTG 2 cut(s) 1261, 1539
Tth111I GACNNNGTC 1 cut(s) 194
Vha464I CTTAAG 1 cut(s) 1202
VpaK11BI GGWCC 3 cut(s) 147, 420, 1459
VspI ATTAAT 1 cut(s) 1368
XceI RCATGY 1 cut(s) 496
XhoI CTCGAG 2 cut(s) 578, 632
XmnI GAANNNNTTC 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.