FvH4_3g41700

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013649
Physical Location & Seq
Forward (+)
34979456 .. 34980891
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41700.t1

Sequence Viewer

Length: 1335 bp
ATGGGTCTGAAATTGATGAATATCCTGACGATACTTGTGCTCTCATGGTTTGCATCTGCAACTACATCCCAAGCTGATGTGTTTGATGTGACTAGTTCAATATATGGCGGAAAGCCCGGCTATGATATTGCAGAGGGTTTGGCAAATGCATGGAGAGATGCATGTTCATCAGAGTCGGCAAGTAAGGTTGTTGTTCCGAGCGGTACGTACAAGTTGAAAGAAGCAATATTTGAAGGTCCTTGCAAGGCTCCGATTGAGGTTCACATCCAAGGAACACTGCAGGCTCCGAAAGACCCAAGTTCAGATGTTTGGGTGACTTTTAGCTTGATTAACATGCTCACACTATCCGGCGGTGGAACCTTCGACGGCCAAGGAGCACATGGTTCAAAACGCAGTGATTTCAGGGACATTAAATTCTCCTCCATCACCAATTCCATAGTCAAGAACATAACTTCACTCAACAGCAAGAAATTCCACATTAGTGTTACCGAGTGCACCAATGTTGTGTTCGACCACCTTACGATTACATCACCTACAGACGATGCTAACACTGATGGAATCGACATTGGGAGTTCGAAAGGGATCAATGTCACTCACACAAACATTGCAACAGGAGATGATTGTATTGCCGTTAGTGAGGGCACCAACCAGCTCCTAGTGACTGGCGTTACTTGCGGACCAGGCCACGGCATAAGCATAGGAAGTCTTGGACAGAAGCCTGATGAACAACCTGTTGTCGGAGTCACAGTCATGAACTGCACCCTTGTTGGCACAACCAATGGTGCGAGAATAAAGACATGGCCTGATTCTCCTGGTGCAAGTACCGCCTCCGATATAGTCTTTGAGGATATTGTAATGGTAAATGTCGAGAATCCTATCATCATTGATCAAAACTACTGCTCAGGAGAAGATGATGATAGATGCAAACAGAATATTCCATCGACAGTTCAGATCAGAAATGTGAGATTCAAGAACATTAGAGGCTCGTCTGCAACTCCAATTGCTGTAAACCTTGCATGTAGTCCTAGTGTGCCATGCCAGAATGTGGAACTAGAAGATGTTGATCTCACATACAGTGGCGACGAAGGCTCCCTCTCATCTCAATGTATCAATGTCAGGCCTAGAATTACTCGCGTCACAGAAGCTCTTGCTTGTGCTAATGTGCCAGGCTTACCATCGGATAATTCACCTCCTCCACCGTTATACGATTATCAATCACCTATGATATACACTGGCGCACTAGACAATAGCAGTTGTTCGTGGCCAAGAGCATCGGTCATGGTGTATCTGCTAATTTCAGGTCTGGGTGTGAGACTGTTGCAACGGTTGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

445

Amino Acids

47.16

Weight (kDa)

4.85

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 373 1.2e-90 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 641
AccB7I CCANNNNNTGG 1 cut(s) 1045
AccBSI CCGCTC 1 cut(s) 201
AccII CGCG 1 cut(s) 1134
AciI CCGC 5 cut(s) 108, 201, 351, 675, 825
AclWI GGATC 1 cut(s) 590
AcoI YGGCCR 2 cut(s) 367, 1262
AcsI RAATTY 2 cut(s) 413, 470
AfaI GTAC 3 cut(s) 205, 209, 823
AfiI CCNNNNNNNGG 3 cut(s) 686, 737, 1045
AgsI TTSAA 5 cut(s) 99, 217, 233, 387, 970
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 3 cut(s) 679, 811, 1165
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AleI CACNNNNGTG 1 cut(s) 480
AluBI AGCT 4 cut(s) 74, 324, 652, 1145
AluI AGCT 4 cut(s) 74, 324, 652, 1145
Alw21I GWGCWC 3 cut(s) 42, 379, 497
Alw26I GTCTC 1 cut(s) 1306
Alw44I GTGCAC 1 cut(s) 493
AlwI GGATC 1 cut(s) 590
AoxI GGCC 5 cut(s) 367, 682, 800, 1118, 1262
ApaLI GTGCAC 1 cut(s) 493
ApoI RAATTY 2 cut(s) 413, 470
AspLEI GCGC 1 cut(s) 1238
AspS9I GGNCC 2 cut(s) 236, 677
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 5 cut(s) 325, 418, 522, 1179, 1209
AsuII TTCGAA 1 cut(s) 575
AvaII GGWCC 2 cut(s) 236, 677
BaeGI GKGCMC 2 cut(s) 497, 644
BalI TGGCCA 1 cut(s) 1264
BanI GGYRCC 1 cut(s) 641
Bbv12I GWGCWC 3 cut(s) 42, 379, 497
BccI CCATC 4 cut(s) 431, 548, 946, 1183
BceAI ACGGC 3 cut(s) 382, 614, 703
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 3 cut(s) 681, 813, 1167
BclI TGATCA 1 cut(s) 886
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 1306
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 6 cut(s) 93, 656, 1026, 1052, 1122, 1241
BfmI CTRYAG 3 cut(s) 278, 534, 1331
Bme1390I CCNGG 4 cut(s) 117, 681, 813, 1167
Bme18I GGWCC 2 cut(s) 236, 677
BmgT120I GGNCC 2 cut(s) 236, 677
BmiI GGNNCC 5 cut(s) 249, 285, 358, 643, 1090
BmrFI CCNGG 4 cut(s) 117, 681, 813, 1167
BmsI GCATC 5 cut(s) 62, 148, 532, 911, 1280
Bpu10I CCTNAGC 1 cut(s) 901
Bpu14I TTCGAA 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 117
BsaAI YACGTR 1 cut(s) 207
BsaBI GATNNNNATC 2 cut(s) 20, 1062
BsaJI CCNNGG 3 cut(s) 268, 370, 685
BsaXI ACNNNNNCTCC 4 cut(s) 732, 762, 1073, 1103
Bsc4I CCNNNNNNNGG 3 cut(s) 686, 737, 1045
Bse1I ACTGG 2 cut(s) 667, 1237
Bse3DI GCAATG 1 cut(s) 603
Bse8I GATNNNNATC 2 cut(s) 20, 1062
BseBI CCWGG 3 cut(s) 681, 813, 1167
BseDI CCNNGG 3 cut(s) 268, 370, 685
BseGI GGATG 2 cut(s) 65, 264
BseJI GATNNNNATC 2 cut(s) 20, 1062
BseLI CCNNNNNNNGG 3 cut(s) 686, 737, 1045
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 1 cut(s) 915
BseNI ACTGG 2 cut(s) 667, 1237
BseRI GAGGAG 2 cut(s) 409, 1182
BseSI GKGCMC 2 cut(s) 497, 644
BsgI GTGCAG 1 cut(s) 742
Bsh1236I CGCG 1 cut(s) 1134
BshFI GGCC 5 cut(s) 369, 684, 802, 1120, 1264
BshNI GGYRCC 1 cut(s) 641
BsiHKAI GWGCWC 3 cut(s) 42, 379, 497
BsiSI CCGG 2 cut(s) 117, 348
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 3 cut(s) 686, 737, 1045
BsmAI GTCTC 1 cut(s) 1306
BsmFI GGGAC 1 cut(s) 419
BsnI GGCC 5 cut(s) 369, 684, 802, 1120, 1264
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 4 cut(s) 42, 379, 497, 644
Bsp143I GATC 4 cut(s) 582, 886, 951, 1063
BspACI CCGC 5 cut(s) 108, 201, 351, 675, 825
BspANI GGCC 5 cut(s) 369, 684, 802, 1120, 1264
BspCNI CTCAG 1 cut(s) 914
BspFNI CGCG 1 cut(s) 1134
BspHI TCATGA 1 cut(s) 750
BspLI GGNNCC 5 cut(s) 249, 285, 358, 643, 1090
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 1 cut(s) 590
BspT104I TTCGAA 1 cut(s) 575
BspT107I GGYRCC 1 cut(s) 641
BsrBI CCGCTC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 2 cut(s) 667, 1237
BssECI CCNNGG 3 cut(s) 268, 370, 685
BssMI GATC 4 cut(s) 582, 886, 951, 1063
BssT1I CCWWGG 2 cut(s) 268, 370
Bst2UI CCWGG 3 cut(s) 681, 813, 1167
Bst4CI ACNGT 6 cut(s) 748, 946, 1076, 1200, 1317, 1326
BstAPI GCANNNNNTGC 1 cut(s) 1327
BstBAI YACGTR 1 cut(s) 207
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 1 cut(s) 901
BstDSI CCRYGG 1 cut(s) 685
BstF5I GGATG 2 cut(s) 65, 264
BstFNI CGCG 1 cut(s) 1134
BstHHI GCGC 1 cut(s) 1238
BstKTI GATC 4 cut(s) 585, 889, 954, 1066
BstMAI GTCTC 1 cut(s) 1306
BstMBI GATC 4 cut(s) 582, 886, 951, 1063
BstMWI GCNNNNNNNGC 5 cut(s) 672, 681, 824, 1086, 1327
BstNI CCWGG 3 cut(s) 681, 813, 1167
BstNSI RCATGY 3 cut(s) 165, 337, 1020
BstSCI CCNGG 4 cut(s) 115, 679, 811, 1165
BstSFI CTRYAG 3 cut(s) 278, 534, 1331
BstSLI GKGCMC 2 cut(s) 497, 644
BstSNI TACGTA 1 cut(s) 207
BstUI CGCG 1 cut(s) 1134
BsuRI GGCC 5 cut(s) 369, 684, 802, 1120, 1264
BtgI CCRYGG 1 cut(s) 685
BtsCI GGATG 2 cut(s) 65, 264
BtsI GCAGTG 2 cut(s) 275, 400
BtsIMutI CAGTG 5 cut(s) 275, 400, 549, 1081, 1230
Cac8I GCNNGC 1 cut(s) 282
CciI TCATGA 1 cut(s) 750
CfoI GCGC 1 cut(s) 1238
Cfr13I GGNCC 2 cut(s) 236, 677
CseI GACGC 1 cut(s) 1123
Csp6I GTAC 3 cut(s) 204, 208, 822
CviQI GTAC 3 cut(s) 204, 208, 822
DdeI CTNAG 1 cut(s) 901
DpnI GATC 4 cut(s) 584, 888, 953, 1065
DpnII GATC 4 cut(s) 582, 886, 951, 1063
EaeI YGGCCR 2 cut(s) 367, 1262
EciI GGCGGA 1 cut(s) 123
Eco105I TACGTA 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 268, 370
Eco147I AGGCCT 1 cut(s) 1120
Eco47I GGWCC 2 cut(s) 236, 677
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 3 cut(s) 679, 811, 1165
EcoT14I CCWWGG 2 cut(s) 268, 370
EcoT22I ATGCAT 2 cut(s) 151, 163
ErhI CCWWGG 2 cut(s) 268, 370
FaqI GGGAC 1 cut(s) 419
FbaI TGATCA 1 cut(s) 886
FokI GGATG 2 cut(s) 52, 251
FspBI CTAG 6 cut(s) 93, 656, 1026, 1052, 1122, 1241
GlaI GCGC 1 cut(s) 1237
HaeIII GGCC 5 cut(s) 369, 684, 802, 1120, 1264
HapII CCGG 2 cut(s) 117, 348
HgaI GACGC 1 cut(s) 1123
HhaI GCGC 1 cut(s) 1238
Hin6I GCGC 1 cut(s) 1236
HinP1I GCGC 1 cut(s) 1236
HinfI GANTC 6 cut(s) 173, 558, 741, 806, 871, 966
HpaII CCGG 2 cut(s) 117, 348
HphI GGTGA 5 cut(s) 325, 418, 522, 1179, 1209
Hpy166II GTNNAC 3 cut(s) 262, 495, 1009
Hpy188III TCNNGA 6 cut(s) 25, 442, 751, 868, 903, 970
Hpy8I GTNNAC 3 cut(s) 262, 495, 1009
Hpy99I CGWCG 2 cut(s) 368, 1085
HpyAV CCTTC 3 cut(s) 227, 370, 1079
HpyCH4III ACNGT 6 cut(s) 748, 946, 1076, 1200, 1317, 1326
HpyCH4IV ACGT 1 cut(s) 206
HpyF10VI GCNNNNNNNGC 5 cut(s) 672, 681, 824, 1086, 1327
HpyF3I CTNAG 1 cut(s) 901
HpySE526I ACGT 1 cut(s) 206
HspAI GCGC 1 cut(s) 1236
Ksp22I TGATCA 1 cut(s) 886
Kzo9I GATC 4 cut(s) 582, 886, 951, 1063
LmnI GCTCC 5 cut(s) 253, 289, 374, 657, 1094
LweI GCATC 5 cut(s) 62, 148, 532, 911, 1280
MaeI CTAG 6 cut(s) 93, 656, 1026, 1052, 1122, 1241
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 8 cut(s) 88, 313, 484, 589, 658, 667, 742, 1135
MalI GATC 4 cut(s) 584, 888, 953, 1065
MbiI CCGCTC 1 cut(s) 201
MboI GATC 4 cut(s) 582, 886, 951, 1063
MboII GAAGA 2 cut(s) 920, 1067
MfeI CAATTG 1 cut(s) 999
MhlI GDGCHC 4 cut(s) 42, 379, 497, 644
MlsI TGGCCA 1 cut(s) 1264
MluCI AATT 8 cut(s) 11, 413, 430, 470, 999, 1125, 1183, 1293
MluNI TGGCCA 1 cut(s) 1264
MlyI GAGTC 2 cut(s) 182, 750
MmeI TCCRAC 1 cut(s) 718
Mox20I TGGCCA 1 cut(s) 1264
Mph1103I ATGCAT 2 cut(s) 151, 163
MscI TGGCCA 1 cut(s) 1264
MseI TTAA 2 cut(s) 330, 411
MslI CAYNNNNRTG 3 cut(s) 480, 749, 1102
Msp20I TGGCCA 1 cut(s) 1264
MspI CCGG 2 cut(s) 117, 348
MspR9I CCNGG 4 cut(s) 117, 681, 813, 1167
MunI CAATTG 1 cut(s) 999
MvaI CCWGG 3 cut(s) 681, 813, 1167
MvnI CGCG 1 cut(s) 1134
MwoI GCNNNNNNNGC 5 cut(s) 672, 681, 824, 1086, 1327
NciI CCSGG 1 cut(s) 117
NdeII GATC 4 cut(s) 582, 886, 951, 1063
NlaIV GGNNCC 5 cut(s) 249, 285, 358, 643, 1090
NmuCI GTSAC 6 cut(s) 88, 313, 589, 658, 742, 1135
NsiI ATGCAT 2 cut(s) 151, 163
NspI RCATGY 3 cut(s) 165, 337, 1020
NspV TTCGAA 1 cut(s) 575
OliI CACNNNNGTG 1 cut(s) 480
PagI TCATGA 1 cut(s) 750
PceI AGGCCT 1 cut(s) 1120
PfeI GAWTC 4 cut(s) 558, 806, 871, 966
PflMI CCANNNNNTGG 1 cut(s) 1045
PleI GAGTC 2 cut(s) 181, 749
PpsI GAGTC 2 cut(s) 181, 749
Ppu21I YACGTR 1 cut(s) 207
PpuMI RGGWCCY 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 3 cut(s) 679, 811, 1165
PspGI CCWGG 3 cut(s) 679, 811, 1165
PspN4I GGNNCC 5 cut(s) 249, 285, 358, 643, 1090
PspPI GGNCC 2 cut(s) 236, 677
PspPPI RGGWCCY 1 cut(s) 236
PstI CTGCAG 1 cut(s) 282
RsaI GTAC 3 cut(s) 205, 209, 823
RsaNI GTAC 3 cut(s) 204, 208, 822
RseI CAYNNNNRTG 3 cut(s) 480, 749, 1102
SaqAI TTAA 2 cut(s) 330, 411
Sau3AI GATC 4 cut(s) 582, 886, 951, 1063
Sau96I GGNCC 2 cut(s) 236, 677
SchI GAGTC 2 cut(s) 182, 750
ScrFI CCNGG 4 cut(s) 117, 681, 813, 1167
SduI GDGCHC 4 cut(s) 42, 379, 497, 644
SfaNI GCATC 5 cut(s) 62, 148, 532, 911, 1280
SfcI CTRYAG 3 cut(s) 278, 534, 1331
SfuI TTCGAA 1 cut(s) 575
SinI GGWCC 2 cut(s) 236, 677
SmiMI CAYNNNNRTG 3 cut(s) 480, 749, 1102
SnaBI TACGTA 1 cut(s) 207
SpeI ACTAGT 1 cut(s) 92
Sse9I AATT 8 cut(s) 11, 413, 430, 470, 999, 1125, 1183, 1293
SseBI AGGCCT 1 cut(s) 1120
SsiI CCGC 5 cut(s) 108, 201, 351, 675, 825
SspI AATATT 2 cut(s) 228, 934
SspMI CTAG 6 cut(s) 93, 656, 1026, 1052, 1122, 1241
StuI AGGCCT 1 cut(s) 1120
StyD4I CCNGG 4 cut(s) 115, 679, 811, 1165
StyI CCWWGG 2 cut(s) 268, 370
TaaI ACNGT 6 cut(s) 748, 946, 1076, 1200, 1317, 1326
TaiI ACGT 1 cut(s) 209
TaqI TCGA 6 cut(s) 363, 510, 561, 575, 867, 941
TaqII GACCGA 1 cut(s) 1264
TasI AATT 8 cut(s) 11, 413, 430, 470, 999, 1125, 1183, 1293
TfiI GAWTC 4 cut(s) 558, 806, 871, 966
Tru1I TTAA 2 cut(s) 330, 411
Tru9I TTAA 2 cut(s) 330, 411
TscAI CASTG 5 cut(s) 282, 400, 556, 1081, 1237
TseFI GTSAC 6 cut(s) 88, 313, 589, 658, 742, 1135
Tsp45I GTSAC 6 cut(s) 88, 313, 589, 658, 742, 1135
TspDTI ATGAA 4 cut(s) 32, 156, 738, 767
TspRI CASTG 5 cut(s) 282, 400, 556, 1081, 1237
Van91I CCANNNNNTGG 1 cut(s) 1045
VneI GTGCAC 1 cut(s) 493
VpaK11BI GGWCC 2 cut(s) 236, 677
XapI RAATTY 2 cut(s) 413, 470
XceI RCATGY 3 cut(s) 165, 337, 1020
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XspI CTAG 6 cut(s) 93, 656, 1026, 1052, 1122, 1241
Zsp2I ATGCAT 2 cut(s) 151, 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.