Rh5DG525200

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013649
Physical Location & Seq
Forward (+)
81317212 .. 81318541
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG525200.1

Sequence Viewer

Length: 876 bp
ATGGGTATCAAAATGGTGAATGTCTTGGCAATACTTGTGCTCTCATTGTTAGCTTCTACAACTACATCCCAAGCCAATGTGTTTGATGTGACTAGTTCAATCTATGGTGGAAAGCCCGGGTCTGATATTGCAAAGGGTTTGGCAAAGGCATGGAGAGATGCATGTGCATCAGAGTCAGCAAGTAAGGTTGTTGTTCCGAGCGGTGCGTACAAGTTAAAAGAAGCAACATTCGAAGGTCCTTGCAAGGCTCCGATTGAGGTTAAGATCCAGGGAACACTGCAGGCTCCAAAAGACCGAAGTTCAGATACTTGGGTTGCTTTTAGCTCCATTGACATGCTCACCTTATGGGGTGGTGGAACTTTTGACGGCCAAGGAGCACATGGTTCGAAAGACGGTGATATCAGAGATATCAGGTTCTCCTCCATCACCAATTCCATAATCATGGACATAACTTCACTAGATAGCAAGAAATACCACATAAGTATTTCCGAGTGCACCAATATTGTGTTTGACCACCTTACCATTACATCGCCTACAAACGATGCTAACACGGATGGAATTGACATTGAGAGTTCAACAAGGATCAATGTCACTCACACAAATATTGCTACTGGAGACGATTGTATTGCCGTTGGCGAAGGCACCAACCAGCTCCTAGTGACCGACGTTACTTGCGGACCAGGCCACGGCATAAGCATAGGAAGTCTTGGATATAAGCCTGATGAAAAGCCCGTGCTTGGAGTCACAATCATGAACTGCACCCTTGCTGGCACAACCAATGGCGCGAGAATAAAGACATGGCCTGATTCTTCTGGTGCTTACCATCGGGTGATTCGCCGCCTCCACCACCGTTATATGATTACCATTCACCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

291

Amino Acids

30.94

Weight (kDa)

6.29

Isoelectric Point (pI)

30.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 273 4e-61 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 641
AccBSI CCGCTC 1 cut(s) 201
AccII CGCG 1 cut(s) 785
AciI CCGC 3 cut(s) 201, 675, 838
AclWI GGATC 2 cut(s) 259, 590
AcoI YGGCCR 1 cut(s) 367
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 2 cut(s) 686, 737
AgsI TTSAA 2 cut(s) 99, 576
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 2 cut(s) 267, 679
AluBI AGCT 3 cut(s) 53, 324, 652
AluI AGCT 3 cut(s) 53, 324, 652
Alw21I GWGCWC 3 cut(s) 42, 379, 497
Alw26I GTCTC 1 cut(s) 609
Alw44I GTGCAC 1 cut(s) 493
AlwI GGATC 2 cut(s) 259, 590
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 3 cut(s) 367, 682, 800
ApaLI GTGCAC 1 cut(s) 493
AspLEI GCGC 1 cut(s) 785
AspS9I GGNCC 2 cut(s) 236, 677
AsuC2I CCSGG 2 cut(s) 117, 118
AsuHPI GGTGA 6 cut(s) 28, 331, 407, 418, 841, 860
AsuII TTCGAA 2 cut(s) 231, 386
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 2 cut(s) 236, 677
BaeGI GKGCMC 1 cut(s) 497
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BanI GGYRCC 1 cut(s) 641
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
Bbv12I GWGCWC 3 cut(s) 42, 379, 497
BccI CCATC 3 cut(s) 431, 548, 831
BceAI ACGGC 3 cut(s) 382, 614, 703
BcgI CGANNNNNNTGC 4 cut(s) 366, 400, 608, 642
BciT130I CCWGG 2 cut(s) 269, 681
BcnI CCSGG 2 cut(s) 117, 118
BcoDI GTCTC 1 cut(s) 609
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 3 cut(s) 93, 458, 656
BfmI CTRYAG 1 cut(s) 278
BisI GCNGC 1 cut(s) 838
BlsI GCNGC 1 cut(s) 839
Bme1390I CCNGG 4 cut(s) 117, 118, 269, 681
Bme18I GGWCC 2 cut(s) 236, 677
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 2 cut(s) 236, 677
BmiI GGNNCC 3 cut(s) 249, 285, 643
BmrFI CCNGG 4 cut(s) 117, 118, 269, 681
BmsI GCATC 3 cut(s) 148, 176, 532
BpmI CTGGAG 1 cut(s) 633
Bpu14I TTCGAA 2 cut(s) 231, 386
BpuMI CCSGG 2 cut(s) 117, 118
BsaJI CCNNGG 4 cut(s) 116, 268, 370, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 686, 737
Bse1I ACTGG 1 cut(s) 616
BseBI CCWGG 2 cut(s) 269, 681
BseDI CCNNGG 4 cut(s) 116, 268, 370, 685
BseGI GGATG 2 cut(s) 65, 559
BseLI CCNNNNNNNGG 2 cut(s) 686, 737
BseNI ACTGG 1 cut(s) 616
BseRI GAGGAG 1 cut(s) 409
BseSI GKGCMC 1 cut(s) 497
BsgI GTGCAG 1 cut(s) 742
Bsh1236I CGCG 1 cut(s) 785
BshFI GGCC 3 cut(s) 369, 684, 802
BshNI GGYRCC 1 cut(s) 641
BsiHKAI GWGCWC 3 cut(s) 42, 379, 497
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 686, 737
BsmAI GTCTC 1 cut(s) 609
BsmBI CGTCTC 1 cut(s) 609
BsnI GGCC 3 cut(s) 369, 684, 802
BsoBI CYCGRG 1 cut(s) 116
Bsp119I TTCGAA 2 cut(s) 231, 386
Bsp1286I GDGCHC 3 cut(s) 42, 379, 497
Bsp143I GATC 2 cut(s) 264, 582
BspACI CCGC 3 cut(s) 201, 675, 838
BspANI GGCC 3 cut(s) 369, 684, 802
BspFNI CGCG 1 cut(s) 785
BspHI TCATGA 1 cut(s) 750
BspLI GGNNCC 3 cut(s) 249, 285, 643
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 2 cut(s) 259, 590
BspT104I TTCGAA 2 cut(s) 231, 386
BspT107I GGYRCC 1 cut(s) 641
BsrBI CCGCTC 1 cut(s) 201
BsrI ACTGG 1 cut(s) 616
BssECI CCNNGG 4 cut(s) 116, 268, 370, 685
BssMI GATC 2 cut(s) 264, 582
BssT1I CCWWGG 1 cut(s) 370
Bst2UI CCWGG 2 cut(s) 269, 681
Bst4CI ACNGT 2 cut(s) 395, 851
BstBI TTCGAA 2 cut(s) 231, 386
BstC8I GCNNGC 2 cut(s) 282, 769
BstDSI CCRYGG 1 cut(s) 685
BstF5I GGATG 2 cut(s) 65, 559
BstFNI CGCG 1 cut(s) 785
BstHHI GCGC 1 cut(s) 785
BstKTI GATC 2 cut(s) 267, 585
BstMAI GTCTC 1 cut(s) 609
BstMBI GATC 2 cut(s) 264, 582
BstMWI GCNNNNNNNGC 1 cut(s) 681
BstNI CCWGG 2 cut(s) 269, 681
BstNSI RCATGY 2 cut(s) 165, 337
BstSCI CCNGG 4 cut(s) 115, 116, 267, 679
BstSFI CTRYAG 1 cut(s) 278
BstSLI GKGCMC 1 cut(s) 497
BstUI CGCG 1 cut(s) 785
BstX2I RGATCY 1 cut(s) 264
BstXI CCANNNNNNTGG 1 cut(s) 442
BstYI RGATCY 1 cut(s) 264
BsuRI GGCC 3 cut(s) 369, 684, 802
BtgI CCRYGG 1 cut(s) 685
BtgZI GCGATG 1 cut(s) 513
BtsCI GGATG 2 cut(s) 65, 559
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 282, 769
CciI TCATGA 1 cut(s) 750
CfoI GCGC 1 cut(s) 785
Cfr13I GGNCC 2 cut(s) 236, 677
Cfr9I CCCGGG 1 cut(s) 116
Csp6I GTAC 1 cut(s) 208
CviAII CATG 8 cut(s) 150, 162, 334, 380, 442, 751, 798, 873
CviQI GTAC 1 cut(s) 208
DpnI GATC 2 cut(s) 266, 584
DpnII GATC 2 cut(s) 264, 582
EaeI YGGCCR 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 370
Eco32I GATATC 2 cut(s) 400, 409
Eco47I GGWCC 2 cut(s) 236, 677
Eco88I CYCGRG 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 267, 679
EcoRV GATATC 2 cut(s) 400, 409
EcoT14I CCWWGG 1 cut(s) 370
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 370
Esp3I CGTCTC 1 cut(s) 609
FaeI CATG 8 cut(s) 153, 165, 337, 383, 445, 754, 801, 876
FatI CATG 8 cut(s) 149, 161, 333, 379, 441, 750, 797, 872
Fnu4HI GCNGC 1 cut(s) 838
FokI GGATG 2 cut(s) 52, 566
Fsp4HI GCNGC 1 cut(s) 838
FspBI CTAG 3 cut(s) 93, 458, 656
GlaI GCGC 1 cut(s) 784
GluI GCNGC 1 cut(s) 838
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 3 cut(s) 369, 684, 802
HapII CCGG 1 cut(s) 117
HhaI GCGC 1 cut(s) 785
Hin1II CATG 8 cut(s) 153, 165, 337, 383, 445, 754, 801, 876
Hin6I GCGC 1 cut(s) 783
HinP1I GCGC 1 cut(s) 783
HinfI GANTC 4 cut(s) 173, 741, 806, 832
HpaII CCGG 1 cut(s) 117
HphI GGTGA 6 cut(s) 28, 331, 407, 418, 841, 860
Hpy166II GTNNAC 1 cut(s) 495
Hpy188I TCNGA 7 cut(s) 124, 172, 198, 252, 304, 404, 490
Hpy188III TCNNGA 1 cut(s) 751
Hpy8I GTNNAC 1 cut(s) 495
Hpy99I CGWCG 1 cut(s) 668
HpyAV CCTTC 2 cut(s) 227, 632
HpyCH4III ACNGT 2 cut(s) 395, 851
HpyCH4IV ACGT 1 cut(s) 666
HpyCH4V TGCA 7 cut(s) 131, 161, 167, 243, 280, 495, 759
HpyF10VI GCNNNNNNNGC 1 cut(s) 681
HpySE526I ACGT 1 cut(s) 666
Hsp92II CATG 8 cut(s) 153, 165, 337, 383, 445, 754, 801, 876
HspAI GCGC 1 cut(s) 783
Kzo9I GATC 2 cut(s) 264, 582
LmnI GCTCC 5 cut(s) 253, 289, 329, 374, 657
LweI GCATC 3 cut(s) 148, 176, 532
MaeI CTAG 3 cut(s) 93, 458, 656
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 5 cut(s) 88, 589, 658, 667, 742
MalI GATC 2 cut(s) 266, 584
MbiI CCGCTC 1 cut(s) 201
MboI GATC 2 cut(s) 264, 582
MboII GAAGA 1 cut(s) 801
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 3 cut(s) 42, 379, 497
MluCI AATT 2 cut(s) 430, 558
MlyI GAGTC 2 cut(s) 182, 750
MnlI CCTC 3 cut(s) 250, 430, 851
Mph1103I ATGCAT 1 cut(s) 163
MseI TTAA 2 cut(s) 215, 261
MslI CAYNNNNRTG 3 cut(s) 332, 440, 749
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 4 cut(s) 117, 118, 269, 681
MvaI CCWGG 2 cut(s) 269, 681
MvnI CGCG 1 cut(s) 785
MwoI GCNNNNNNNGC 1 cut(s) 681
NciI CCSGG 2 cut(s) 117, 118
NdeII GATC 2 cut(s) 264, 582
NlaIII CATG 8 cut(s) 153, 165, 337, 383, 445, 754, 801, 876
NlaIV GGNNCC 3 cut(s) 249, 285, 643
NmuCI GTSAC 4 cut(s) 88, 589, 658, 742
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 2 cut(s) 165, 337
NspV TTCGAA 2 cut(s) 231, 386
PagI TCATGA 1 cut(s) 750
PfeI GAWTC 2 cut(s) 806, 832
PkrI GCNGC 1 cut(s) 839
PleI GAGTC 2 cut(s) 181, 749
PpsI GAGTC 2 cut(s) 181, 749
PpuMI RGGWCCY 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 2 cut(s) 267, 679
PspGI CCWGG 2 cut(s) 267, 679
PspN4I GGNNCC 3 cut(s) 249, 285, 643
PspPI GGNCC 2 cut(s) 236, 677
PspPPI RGGWCCY 1 cut(s) 236
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 264
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 3 cut(s) 332, 440, 749
SaqAI TTAA 2 cut(s) 215, 261
SatI GCNGC 1 cut(s) 838
Sau3AI GATC 2 cut(s) 264, 582
Sau96I GGNCC 2 cut(s) 236, 677
SchI GAGTC 2 cut(s) 182, 750
ScrFI CCNGG 4 cut(s) 117, 118, 269, 681
SduI GDGCHC 3 cut(s) 42, 379, 497
SfaNI GCATC 3 cut(s) 148, 176, 532
SfcI CTRYAG 1 cut(s) 278
SfuI TTCGAA 2 cut(s) 231, 386
SinI GGWCC 2 cut(s) 236, 677
SmaI CCCGGG 1 cut(s) 118
SmiMI CAYNNNNRTG 3 cut(s) 332, 440, 749
SpeI ACTAGT 1 cut(s) 92
Sse9I AATT 2 cut(s) 430, 558
SsiI CCGC 3 cut(s) 201, 675, 838
SspI AATATT 2 cut(s) 502, 604
SspMI CTAG 3 cut(s) 93, 458, 656
StyD4I CCNGG 4 cut(s) 115, 116, 267, 679
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 2 cut(s) 395, 851
TaiI ACGT 1 cut(s) 669
TaqI TCGA 2 cut(s) 231, 386
TaqII GACCGA 2 cut(s) 309, 677
TasI AATT 2 cut(s) 430, 558
TauI GCSGC 1 cut(s) 840
TfiI GAWTC 2 cut(s) 806, 832
Tru1I TTAA 2 cut(s) 215, 261
Tru9I TTAA 2 cut(s) 215, 261
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 4 cut(s) 88, 589, 658, 742
Tsp45I GTSAC 4 cut(s) 88, 589, 658, 742
TspDTI ATGAA 2 cut(s) 738, 767
TspGWI ACGGA 1 cut(s) 566
TspMI CCCGGG 1 cut(s) 116
TspRI CASTG 1 cut(s) 282
VneI GTGCAC 1 cut(s) 493
VpaK11BI GGWCC 2 cut(s) 236, 677
XceI RCATGY 2 cut(s) 165, 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XmaI CCCGGG 1 cut(s) 116
XspI CTAG 3 cut(s) 93, 458, 656
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.