Rroxscaffold_1G00006790

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013649
Physical Location & Seq
Reverse (-)
8777210 .. 8778022
813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006790.1

Sequence Viewer

Length: 702 bp
ATGGGTCTGAAATTGGTGAATGTCCTGGCAATACTGGTGCTCTCATTGTTAGCTTCTACAACTACATCCCAAGCCGATGTGTTTGATGTGACTAGTTCAATCTATGGTGGAAAGCCCGGCTCTGATATTGCAGAGGGTTTGGCAAAGGCATGGAGAGATGCATGTGCATCAGAGTCGTCAAGTAAGGTTGTTGTTCCGAGCGGTGCGTTCAAGTTGAAAGAAGCAACATTCGAAGGTCCTTGCAAGGCTCCGATTGAGGTTAAGATCCAAGGAACACTGCAGGCTCCAAAAGACCGAAGTTCAGATACTTGGGTTGCTTTTAGCTCCATTGACATGCTCACCTTATGGGGTGGTGGAACTTTTGACGGCCAAGGAGCACATGGTTCGAAAGACGGTGATATCAGAGATATCAGGTTCTCCTCCATCACCAATTCCATAATCATGGACATAACTTCACTAGATAGCAAGAAATACCACATAAGTATTTCCGAGTGCACCAATATTGTGTTCGACCACCTTACCATTACATCGCCTACAAACGATGCTAACACGGATGGAATTGACATTGGGAGTTCAACAAGGATCAATGTCACACACACAAATATTGCTACCGGAGACGATTGTATTGCCATTGGCGAAGGCACCAACCAGCTCCTAGTGACCGACGTTACTTGCGGACCAGGCCACGGCATAAGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

233

Amino Acids

24.4

Weight (kDa)

4.79

Isoelectric Point (pI)

30.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 233 3.1e-46 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 641
AccBSI CCGCTC 1 cut(s) 201
AciI CCGC 2 cut(s) 201, 675
AclWI GGATC 2 cut(s) 259, 590
AcoI YGGCCR 1 cut(s) 367
AfiI CCNNNNNNNGG 1 cut(s) 686
AgsI TTSAA 4 cut(s) 99, 211, 217, 576
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 2 cut(s) 24, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AluBI AGCT 3 cut(s) 53, 324, 652
AluI AGCT 3 cut(s) 53, 324, 652
Alw21I GWGCWC 3 cut(s) 42, 379, 497
Alw26I GTCTC 1 cut(s) 609
Alw44I GTGCAC 1 cut(s) 493
AlwI GGATC 2 cut(s) 259, 590
AoxI GGCC 2 cut(s) 367, 682
ApaLI GTGCAC 1 cut(s) 493
AspS9I GGNCC 2 cut(s) 236, 677
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 4 cut(s) 28, 331, 407, 418
AsuII TTCGAA 2 cut(s) 231, 386
AvaII GGWCC 2 cut(s) 236, 677
BaeGI GKGCMC 1 cut(s) 497
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BanI GGYRCC 1 cut(s) 641
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
Bbv12I GWGCWC 3 cut(s) 42, 379, 497
BccI CCATC 2 cut(s) 431, 548
BceAI ACGGC 1 cut(s) 382
BcgI CGANNNNNNTGC 6 cut(s) 156, 190, 366, 400, 608, 642
BciT130I CCWGG 2 cut(s) 26, 681
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 609
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 3 cut(s) 93, 458, 656
BfmI CTRYAG 1 cut(s) 278
Bme1390I CCNGG 3 cut(s) 26, 117, 681
Bme18I GGWCC 2 cut(s) 236, 677
BmgT120I GGNCC 2 cut(s) 236, 677
BmiI GGNNCC 3 cut(s) 249, 285, 643
BmrFI CCNGG 3 cut(s) 26, 117, 681
BmsI GCATC 3 cut(s) 148, 176, 532
Bpu14I TTCGAA 2 cut(s) 231, 386
BpuMI CCSGG 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 268, 370, 685
BsaWI WCCGGW 1 cut(s) 611
Bsc4I CCNNNNNNNGG 1 cut(s) 686
Bse1I ACTGG 1 cut(s) 39
BseBI CCWGG 2 cut(s) 26, 681
BseDI CCNNGG 3 cut(s) 268, 370, 685
BseGI GGATG 2 cut(s) 65, 559
BseLI CCNNNNNNNGG 1 cut(s) 686
BseNI ACTGG 1 cut(s) 39
BseRI GAGGAG 1 cut(s) 409
BseSI GKGCMC 1 cut(s) 497
BshFI GGCC 2 cut(s) 369, 684
BshNI GGYRCC 1 cut(s) 641
BsiHKAI GWGCWC 3 cut(s) 42, 379, 497
BsiSI CCGG 2 cut(s) 117, 612
BslI CCNNNNNNNGG 1 cut(s) 686
BsmAI GTCTC 1 cut(s) 609
BsmBI CGTCTC 1 cut(s) 609
BsnI GGCC 2 cut(s) 369, 684
Bsp119I TTCGAA 2 cut(s) 231, 386
Bsp1286I GDGCHC 3 cut(s) 42, 379, 497
Bsp143I GATC 2 cut(s) 264, 582
BspACI CCGC 2 cut(s) 201, 675
BspANI GGCC 2 cut(s) 369, 684
BspLI GGNNCC 3 cut(s) 249, 285, 643
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 2 cut(s) 259, 590
BspT104I TTCGAA 2 cut(s) 231, 386
BspT107I GGYRCC 1 cut(s) 641
BsrBI CCGCTC 1 cut(s) 201
BsrI ACTGG 1 cut(s) 39
BssECI CCNNGG 3 cut(s) 268, 370, 685
BssMI GATC 2 cut(s) 264, 582
BssT1I CCWWGG 2 cut(s) 268, 370
Bst2UI CCWGG 2 cut(s) 26, 681
Bst4CI ACNGT 1 cut(s) 395
BstBI TTCGAA 2 cut(s) 231, 386
BstC8I GCNNGC 1 cut(s) 282
BstDSI CCRYGG 1 cut(s) 685
BstF5I GGATG 2 cut(s) 65, 559
BstKTI GATC 2 cut(s) 267, 585
BstMAI GTCTC 1 cut(s) 609
BstMBI GATC 2 cut(s) 264, 582
BstMWI GCNNNNNNNGC 1 cut(s) 681
BstNI CCWGG 2 cut(s) 26, 681
BstNSI RCATGY 2 cut(s) 165, 337
BstSCI CCNGG 3 cut(s) 24, 115, 679
BstSFI CTRYAG 1 cut(s) 278
BstSLI GKGCMC 1 cut(s) 497
BstX2I RGATCY 1 cut(s) 264
BstXI CCANNNNNNTGG 1 cut(s) 442
BstYI RGATCY 1 cut(s) 264
BsuRI GGCC 2 cut(s) 369, 684
BtgI CCRYGG 1 cut(s) 685
BtgZI GCGATG 1 cut(s) 513
BtsCI GGATG 2 cut(s) 65, 559
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 1 cut(s) 282
Cfr13I GGNCC 2 cut(s) 236, 677
CviAII CATG 5 cut(s) 150, 162, 334, 380, 442
DpnI GATC 2 cut(s) 266, 584
DpnII GATC 2 cut(s) 264, 582
EaeI YGGCCR 1 cut(s) 367
Eco130I CCWWGG 2 cut(s) 268, 370
Eco32I GATATC 2 cut(s) 400, 409
Eco47I GGWCC 2 cut(s) 236, 677
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 24, 679
EcoRV GATATC 2 cut(s) 400, 409
EcoT14I CCWWGG 2 cut(s) 268, 370
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 268, 370
Esp3I CGTCTC 1 cut(s) 609
FaeI CATG 5 cut(s) 153, 165, 337, 383, 445
FatI CATG 5 cut(s) 149, 161, 333, 379, 441
FauNDI CATATG 1 cut(s) 698
FokI GGATG 2 cut(s) 52, 566
FspBI CTAG 3 cut(s) 93, 458, 656
HaeIII GGCC 2 cut(s) 369, 684
HapII CCGG 2 cut(s) 117, 612
Hin1II CATG 5 cut(s) 153, 165, 337, 383, 445
HinfI GANTC 1 cut(s) 173
HpaII CCGG 2 cut(s) 117, 612
HphI GGTGA 4 cut(s) 28, 331, 407, 418
Hpy166II GTNNAC 1 cut(s) 495
Hpy188I TCNGA 8 cut(s) 9, 124, 172, 198, 252, 304, 404, 490
Hpy8I GTNNAC 1 cut(s) 495
Hpy99I CGWCG 1 cut(s) 668
HpyAV CCTTC 2 cut(s) 227, 632
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 1 cut(s) 666
HpyCH4V TGCA 6 cut(s) 131, 161, 167, 243, 280, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 681
HpySE526I ACGT 1 cut(s) 666
Hsp92II CATG 5 cut(s) 153, 165, 337, 383, 445
Kzo9I GATC 2 cut(s) 264, 582
LmnI GCTCC 5 cut(s) 253, 289, 329, 374, 657
LweI GCATC 3 cut(s) 148, 176, 532
MaeI CTAG 3 cut(s) 93, 458, 656
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 4 cut(s) 88, 589, 658, 667
MalI GATC 2 cut(s) 266, 584
MbiI CCGCTC 1 cut(s) 201
MboI GATC 2 cut(s) 264, 582
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 3 cut(s) 42, 379, 497
MluCI AATT 3 cut(s) 11, 430, 558
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 3 cut(s) 127, 250, 430
Mph1103I ATGCAT 1 cut(s) 163
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 2 cut(s) 332, 440
MspI CCGG 2 cut(s) 117, 612
MspR9I CCNGG 3 cut(s) 26, 117, 681
MvaI CCWGG 2 cut(s) 26, 681
MwoI GCNNNNNNNGC 1 cut(s) 681
NciI CCSGG 1 cut(s) 117
NdeI CATATG 1 cut(s) 698
NdeII GATC 2 cut(s) 264, 582
NlaIII CATG 5 cut(s) 153, 165, 337, 383, 445
NlaIV GGNNCC 3 cut(s) 249, 285, 643
NmuCI GTSAC 3 cut(s) 88, 589, 658
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 2 cut(s) 165, 337
NspV TTCGAA 2 cut(s) 231, 386
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PpuMI RGGWCCY 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 2 cut(s) 24, 679
PspGI CCWGG 2 cut(s) 24, 679
PspN4I GGNNCC 3 cut(s) 249, 285, 643
PspPI GGNCC 2 cut(s) 236, 677
PspPPI RGGWCCY 1 cut(s) 236
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 264
RseI CAYNNNNRTG 2 cut(s) 332, 440
SaqAI TTAA 1 cut(s) 261
Sau3AI GATC 2 cut(s) 264, 582
Sau96I GGNCC 2 cut(s) 236, 677
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 26, 117, 681
SduI GDGCHC 3 cut(s) 42, 379, 497
SfaNI GCATC 3 cut(s) 148, 176, 532
SfcI CTRYAG 1 cut(s) 278
SfuI TTCGAA 2 cut(s) 231, 386
SinI GGWCC 2 cut(s) 236, 677
SmiMI CAYNNNNRTG 2 cut(s) 332, 440
SpeI ACTAGT 1 cut(s) 92
Sse9I AATT 3 cut(s) 11, 430, 558
SsiI CCGC 2 cut(s) 201, 675
SspI AATATT 2 cut(s) 502, 604
SspMI CTAG 3 cut(s) 93, 458, 656
StyD4I CCNGG 3 cut(s) 24, 115, 679
StyI CCWWGG 2 cut(s) 268, 370
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 1 cut(s) 669
TaqI TCGA 3 cut(s) 231, 386, 510
TaqII GACCGA 2 cut(s) 309, 677
TasI AATT 3 cut(s) 11, 430, 558
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 3 cut(s) 88, 589, 658
Tsp45I GTSAC 3 cut(s) 88, 589, 658
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 1 cut(s) 282
VneI GTGCAC 1 cut(s) 493
VpaK11BI GGWCC 2 cut(s) 236, 677
XceI RCATGY 2 cut(s) 165, 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XspI CTAG 3 cut(s) 93, 458, 656
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.