Rroxscaffold_1G00006780

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013649
Physical Location & Seq
Reverse (-)
8776198 .. 8777161
964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006780.1

Sequence Viewer

Length: 501 bp
ATGAACTGCACCCTTGTTGGCACAACCAATGGCGCCAGAATAAAGACATGGCCTGATTCTCCTGGTGTAAGTACTGCCTCGGATATCCTCTTTGAGGATATTGTAATGGTGAATGTCGAGAATCCTATCATCATTGACCAAAACTACTGCTCGGGAGATGAGGATAGATGCAAACAGAATACTCCGTCGGAAGTTCAGATCAGTAATGTGAGGTTCAAGAACATCAGAGGCTCGTCTGCAACTCCAATTGCTGTAAACCTTGCATGTAGTCCGCGCGTGCCATGCCAGAACGTGGAACTGGAAGATATTGATCTCATATACAATGGCAACAAAGGCTCTCTTTCGTCTCAATGTTTCAATGCCAGGCCTAGAACCACTCGCGTCGGAAAAGCTCTTGCTTGTGCTAATCTACCATCGGGTGATTCGCCGCCTCCACCACCCTTTAATCTACTATCTACCAATGAAGAATCGGCACACAAGCAGCAAAAGAAGGCAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

166

Amino Acids

17.9

Weight (kDa)

5.82

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 2 - 122 9.3e-30 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 32
AccB7I CCANNNNNTGG 1 cut(s) 292
AccII CGCG 3 cut(s) 274, 276, 381
AciI CCGC 2 cut(s) 272, 428
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 2 cut(s) 94, 292
AgsI TTSAA 2 cut(s) 217, 358
AjnI CCWGG 2 cut(s) 61, 362
AluBI AGCT 1 cut(s) 392
AluI AGCT 1 cut(s) 392
Alw26I GTCTC 1 cut(s) 351
Ama87I CYCGRG 1 cut(s) 151
AoxI GGCC 2 cut(s) 50, 365
ApeKI GCWGC 2 cut(s) 481, 494
AspLEI GCGC 2 cut(s) 35, 276
AsuHPI GGTGA 2 cut(s) 121, 431
AvaI CYCGRG 1 cut(s) 151
BanI GGYRCC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 493
BccI CCATC 1 cut(s) 421
BciT130I CCWGG 2 cut(s) 63, 364
BcoDI GTCTC 1 cut(s) 351
BfaI CTAG 1 cut(s) 369
BfoI RGCGCY 1 cut(s) 36
BisI GCNGC 3 cut(s) 428, 482, 495
BlsI GCNGC 3 cut(s) 429, 483, 496
BmcAI AGTACT 1 cut(s) 73
Bme1390I CCNGG 2 cut(s) 63, 364
BmeT110I CYCGRG 1 cut(s) 151
BmiI GGNNCC 1 cut(s) 34
BmrFI CCNGG 2 cut(s) 63, 364
BmsI GCATC 1 cut(s) 158
BsaBI GATNNNNATC 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 78
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 292
Bse1I ACTGG 1 cut(s) 303
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 2 cut(s) 63, 364
BseDI CCNNGG 1 cut(s) 78
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 2 cut(s) 94, 292
BseNI ACTGG 1 cut(s) 303
BseXI GCAGC 1 cut(s) 493
Bsh1236I CGCG 3 cut(s) 274, 276, 381
BshFI GGCC 2 cut(s) 52, 367
BshNI GGYRCC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 151
BslI CCNNNNNNNGG 2 cut(s) 94, 292
BsmAI GTCTC 1 cut(s) 351
BsmBI CGTCTC 1 cut(s) 351
BsnI GGCC 2 cut(s) 52, 367
BsoBI CYCGRG 1 cut(s) 151
Bsp143I GATC 2 cut(s) 198, 310
BspACI CCGC 2 cut(s) 272, 428
BspANI GGCC 2 cut(s) 52, 367
BspFNI CGCG 3 cut(s) 274, 276, 381
BspLI GGNNCC 1 cut(s) 34
BspT107I GGYRCC 1 cut(s) 32
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 2 cut(s) 198, 310
BssNI GRCGYC 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 63, 364
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 278
BstENI CCTNNNNNAGG 1 cut(s) 92
BstFNI CGCG 3 cut(s) 274, 276, 381
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 276
BstKTI GATC 2 cut(s) 201, 313
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 2 cut(s) 198, 310
BstMWI GCNNNNNNNGC 2 cut(s) 282, 333
BstNI CCWGG 2 cut(s) 63, 364
BstNSI RCATGY 1 cut(s) 267
BstSCI CCNGG 2 cut(s) 61, 362
BstUI CGCG 3 cut(s) 274, 276, 381
BstV1I GCAGC 1 cut(s) 493
BsuRI GGCC 2 cut(s) 52, 367
Cac8I GCNNGC 1 cut(s) 278
CfoI GCGC 2 cut(s) 35, 276
CseI GACGC 1 cut(s) 370
Csp6I GTAC 1 cut(s) 72
CviAII CATG 3 cut(s) 48, 264, 282
CviJI RGCY 5 cut(s) 52, 231, 336, 367, 392
CviKI_1 RGCY 5 cut(s) 52, 231, 336, 367, 392
CviQI GTAC 1 cut(s) 72
DinI GGCGCC 1 cut(s) 34
DpnI GATC 2 cut(s) 200, 312
DpnII GATC 2 cut(s) 198, 310
Eco147I AGGCCT 1 cut(s) 367
Eco32I GATATC 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 151
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoRII CCWGG 2 cut(s) 61, 362
EcoRV GATATC 1 cut(s) 85
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
Esp3I CGTCTC 1 cut(s) 351
FaeI CATG 3 cut(s) 51, 267, 285
FaiI YATR 6 cut(s) 49, 265, 283, 317, 319, 499
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FatI CATG 3 cut(s) 47, 263, 281
Fnu4HI GCNGC 3 cut(s) 428, 482, 495
Fsp4HI GCNGC 3 cut(s) 428, 482, 495
FspBI CTAG 1 cut(s) 369
GlaI GCGC 2 cut(s) 34, 275
GluI GCNGC 3 cut(s) 428, 482, 495
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 2 cut(s) 52, 367
HgaI GACGC 1 cut(s) 370
HhaI GCGC 2 cut(s) 35, 276
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 3 cut(s) 51, 267, 285
Hin6I GCGC 2 cut(s) 33, 274
HinP1I GCGC 2 cut(s) 33, 274
HinfI GANTC 4 cut(s) 56, 121, 422, 467
HphI GGTGA 2 cut(s) 121, 431
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 5 cut(s) 82, 190, 198, 227, 386
Hpy188III TCNNGA 3 cut(s) 118, 153, 217
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 2 cut(s) 190, 386
HpyAV CCTTC 1 cut(s) 484
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 4 cut(s) 9, 171, 239, 263
HpyF10VI GCNNNNNNNGC 2 cut(s) 282, 333
HpySE526I ACGT 1 cut(s) 291
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 3 cut(s) 51, 267, 285
HspAI GCGC 2 cut(s) 33, 274
KasI GGCGCC 1 cut(s) 32
Kzo9I GATC 2 cut(s) 198, 310
LpnPI CCDG 8 cut(s) 48, 49, 66, 75, 284, 299, 349, 376
Lsp1109I GCAGC 1 cut(s) 493
LweI GCATC 1 cut(s) 158
MaeI CTAG 1 cut(s) 369
MaeII ACGT 1 cut(s) 291
MalI GATC 2 cut(s) 200, 312
MboI GATC 2 cut(s) 198, 310
MboII GAAGA 2 cut(s) 314, 476
MfeI CAATTG 1 cut(s) 246
MluCI AATT 1 cut(s) 246
Mly113I GGCGCC 1 cut(s) 33
MmeI TCCRAC 2 cut(s) 168, 364
MnlI CCTC 7 cut(s) 88, 88, 98, 154, 204, 221, 441
MseI TTAA 1 cut(s) 444
MspR9I CCNGG 2 cut(s) 63, 364
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 2 cut(s) 63, 364
MvnI CGCG 3 cut(s) 274, 276, 381
MwoI GCNNNNNNNGC 2 cut(s) 282, 333
NarI GGCGCC 1 cut(s) 33
NdeII GATC 2 cut(s) 198, 310
NlaIII CATG 3 cut(s) 51, 267, 285
NlaIV GGNNCC 1 cut(s) 34
NspI RCATGY 1 cut(s) 267
PceI AGGCCT 1 cut(s) 367
PfeI GAWTC 4 cut(s) 56, 121, 422, 467
PflMI CCANNNNNTGG 1 cut(s) 292
PkrI GCNGC 3 cut(s) 429, 483, 496
PluTI GGCGCC 1 cut(s) 36
Psp6I CCWGG 2 cut(s) 61, 362
PspGI CCWGG 2 cut(s) 61, 362
PspN4I GGNNCC 1 cut(s) 34
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 3 cut(s) 428, 482, 495
Sau3AI GATC 2 cut(s) 198, 310
ScaI AGTACT 1 cut(s) 73
ScrFI CCNGG 2 cut(s) 63, 364
SetI ASST 4 cut(s) 215, 261, 294, 394
SfaNI GCATC 1 cut(s) 158
SfoI GGCGCC 1 cut(s) 34
Sse9I AATT 1 cut(s) 246
SseBI AGGCCT 1 cut(s) 367
SsiI CCGC 2 cut(s) 272, 428
SspDI GGCGCC 1 cut(s) 32
SspMI CTAG 1 cut(s) 369
StuI AGGCCT 1 cut(s) 367
StyD4I CCNGG 2 cut(s) 61, 362
TaiI ACGT 1 cut(s) 294
TaqI TCGA 1 cut(s) 117
TasI AATT 1 cut(s) 246
TatI WGTACW 1 cut(s) 71
TauI GCSGC 1 cut(s) 430
TfiI GAWTC 4 cut(s) 56, 121, 422, 467
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TseI GCWGC 2 cut(s) 481, 494
TspDTI ATGAA 2 cut(s) 17, 477
TspGWI ACGGA 1 cut(s) 174
Van91I CCANNNNNTGG 1 cut(s) 292
XagI CCTNNNNNAGG 1 cut(s) 92
XceI RCATGY 1 cut(s) 267
XspI CTAG 1 cut(s) 369
ZrmI AGTACT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.