Rw5G045540

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013649
Physical Location & Seq
Forward (+)
79551220 .. 79552663
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045540.1

Sequence Viewer

Length: 1260 bp
ATGTACGTCCTGGCAATACTTGTGCTCTCATTGTTAGCGTCTACAACTACATCCCAAGCCGATGTGTTTGATGTGACTAGTTCAATCTATGGTGGAAAGCCCGGCTCTGATATTACAGAGGGTTTGGCAAAGGCATGGAGAGATGCATGTGCATCAGAGTCGTCAAGTAAGGTTGTTGTTCCAAGCGGTGCGTACAAGTTGAAAGAAGCAACATTCGAAGGTCCTTGCAAGGCTCCGATTGAGGTTAAGATCCAAGGAACACTGCAGGCTCCAAAAGACCGAAGAGCACATGGTTCGAAAGACGGTGATATCAGAGATATCAGGTTCTCCTCCATCACCAATTCCATAATCATGGACATAACTTCACTAGATAGTAAGAAATACCACATAAGTATTTCCGAGTGCACCAATATTGTGTTCGACCACCTTACCATTACATCGCCTACAAACGATGCTAACACGGATGGAATTGACATTGGGAGTTCAACAAGGATCAATGTCACTCACACAAATATTGCTACCGGAGACGATTGTATTGCCGTTGGCGAAGGCACCAACCAGCTCCTAGTGACCGACGTTACTTGCGGACCAGGCCACGGCATAAGCATAGGAAGTCTTGGATATAAGGCTGATGAAAAGCCCGTGTTTGGAGTCACAATCATGAACTGCACCCTTGTTGGCACAACCAATGGCGCGAGAATAAAGACATGGCCTGATTCTCCTGGTGTTAGTGCCTCGGATATCCTCTTTGAGGATATTGTAATGGTGAATGTCGAGAATCCTATCATCATTGACCAAAACTACTGCTCGGGAGATGAGGATAGATGCAAACAGAATACTCCGTCGGAAGTTCAGATCAGTAATGTGAGGTTCAAGAACATCAAAGGCTCGTCTGCAACTCCAATTGCTGTAAACCTTGCATGTAGTCCGATCGTGCCATGCCAGAATGTGGAACTGGAAGATATTGATCTTACATACAATGGCAACGAAGGCTCTCTTTCGTCTCAATGTTTCAATGTCAGGCCTAGAACCACTCGCGTCGGAAAACCTCTTGCTTGTGCTAATCTACCAGGCTTACCATCGGGTGATTCGCCGCCTCCACCACCGTTATATGATTACCATTCACCTATGATATACACTGGCGGCCCAGACAATAACAGTTGTTGGTGGCCAAGACCACCGGTCATGGGGTATCTACTATTTTCGGGTCTGGTTGTGTGGCTGTTGCAAGAGTTGCTACAAGAATACAGCAAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

419

Amino Acids

44.94

Weight (kDa)

4.88

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 106 - 341 3.6e-72 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 551
AccB7I CCANNNNNTGG 1 cut(s) 949
AccI GTMKAC 1 cut(s) 41
AccII CGCG 2 cut(s) 695, 1038
AciI CCGC 4 cut(s) 186, 585, 1094, 1143
AclWI GGATC 2 cut(s) 244, 500
AcoI YGGCCR 1 cut(s) 1169
AfaI GTAC 2 cut(s) 5, 194
AfiI CCNNNNNNNGG 5 cut(s) 596, 647, 751, 949, 1187
AgeI ACCGGT 1 cut(s) 1180
AgsI TTSAA 5 cut(s) 84, 202, 486, 874, 1015
AhdI GACNNNNNGTC 1 cut(s) 1181
AhlI ACTAGT 1 cut(s) 77
AjnI CCWGG 4 cut(s) 9, 589, 721, 1069
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AluBI AGCT 1 cut(s) 562
AluI AGCT 1 cut(s) 562
Alw21I GWGCWC 3 cut(s) 27, 289, 407
Alw26I GTCTC 2 cut(s) 519, 1008
Alw44I GTGCAC 1 cut(s) 403
AlwI GGATC 2 cut(s) 244, 500
Ama87I CYCGRG 1 cut(s) 808
AoxI GGCC 5 cut(s) 592, 710, 1022, 1144, 1169
ApaLI GTGCAC 1 cut(s) 403
AsiGI ACCGGT 1 cut(s) 1180
AspLEI GCGC 1 cut(s) 695
AspS9I GGNCC 3 cut(s) 221, 587, 1145
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 5 cut(s) 317, 328, 778, 1097, 1116
AsuII TTCGAA 2 cut(s) 216, 296
AvaI CYCGRG 1 cut(s) 808
AvaII GGWCC 2 cut(s) 221, 587
BaeGI GKGCMC 1 cut(s) 407
BalI TGGCCA 1 cut(s) 1171
BanI GGYRCC 1 cut(s) 551
Bbv12I GWGCWC 3 cut(s) 27, 289, 407
BccI CCATC 3 cut(s) 341, 458, 1087
BceAI ACGGC 2 cut(s) 524, 613
BcgI CGANNNNNNTGC 6 cut(s) 141, 175, 276, 310, 518, 552
BciT130I CCWGG 4 cut(s) 11, 591, 723, 1071
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 2 cut(s) 519, 1008
BcuI ACTAGT 1 cut(s) 77
BfaI CTAG 4 cut(s) 78, 368, 566, 1026
BfmI CTRYAG 2 cut(s) 263, 1256
BisI GCNGC 2 cut(s) 1094, 1144
BlsI GCNGC 2 cut(s) 1095, 1145
Bme1390I CCNGG 5 cut(s) 11, 102, 591, 723, 1071
Bme18I GGWCC 2 cut(s) 221, 587
BmeRI GACNNNNNGTC 1 cut(s) 1181
BmeT110I CYCGRG 1 cut(s) 808
BmgT120I GGNCC 3 cut(s) 221, 587, 1145
BmiI GGNNCC 3 cut(s) 234, 270, 553
BmrFI CCNGG 5 cut(s) 11, 102, 591, 723, 1071
BmsI GCATC 4 cut(s) 133, 161, 442, 815
Bpu14I TTCGAA 2 cut(s) 216, 296
BpuMI CCSGG 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 966
BsaJI CCNNGG 3 cut(s) 253, 595, 735
BsaWI WCCGGW 2 cut(s) 521, 1180
Bsc4I CCNNNNNNNGG 5 cut(s) 596, 647, 751, 949, 1187
Bse118I RCCGGY 1 cut(s) 1180
Bse1I ACTGG 2 cut(s) 960, 1144
Bse8I GATNNNNATC 1 cut(s) 966
BseBI CCWGG 4 cut(s) 11, 591, 723, 1071
BseDI CCNNGG 3 cut(s) 253, 595, 735
BseGI GGATG 2 cut(s) 50, 469
BseJI GATNNNNATC 1 cut(s) 966
BseLI CCNNNNNNNGG 5 cut(s) 596, 647, 751, 949, 1187
BseNI ACTGG 2 cut(s) 960, 1144
BseRI GAGGAG 1 cut(s) 319
BseSI GKGCMC 1 cut(s) 407
BsgI GTGCAG 1 cut(s) 652
Bsh1236I CGCG 2 cut(s) 695, 1038
Bsh1285I CGRYCG 1 cut(s) 933
BshFI GGCC 5 cut(s) 594, 712, 1024, 1146, 1171
BshNI GGYRCC 1 cut(s) 551
BshTI ACCGGT 1 cut(s) 1180
BsiEI CGRYCG 1 cut(s) 933
BsiHKAI GWGCWC 3 cut(s) 27, 289, 407
BsiHKCI CYCGRG 1 cut(s) 808
BsiSI CCGG 3 cut(s) 102, 522, 1181
BslI CCNNNNNNNGG 5 cut(s) 596, 647, 751, 949, 1187
BsmAI GTCTC 2 cut(s) 519, 1008
BsmBI CGTCTC 2 cut(s) 519, 1008
BsnI GGCC 5 cut(s) 594, 712, 1024, 1146, 1171
BsoBI CYCGRG 1 cut(s) 808
Bsp119I TTCGAA 2 cut(s) 216, 296
Bsp1286I GDGCHC 3 cut(s) 27, 289, 407
Bsp143I GATC 5 cut(s) 249, 492, 855, 930, 967
BspACI CCGC 4 cut(s) 186, 585, 1094, 1143
BspANI GGCC 5 cut(s) 594, 712, 1024, 1146, 1171
BspFNI CGCG 2 cut(s) 695, 1038
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 3 cut(s) 234, 270, 553
BspMAI CTGCAG 1 cut(s) 267
BspPI GGATC 2 cut(s) 244, 500
BspQI GCTCTTC 1 cut(s) 277
BspT104I TTCGAA 2 cut(s) 216, 296
BspT107I GGYRCC 1 cut(s) 551
BsrFI RCCGGY 1 cut(s) 1180
BsrI ACTGG 2 cut(s) 960, 1144
BssAI RCCGGY 1 cut(s) 1180
BssECI CCNNGG 3 cut(s) 253, 595, 735
BssMI GATC 5 cut(s) 249, 492, 855, 930, 967
BssT1I CCWWGG 1 cut(s) 253
Bst2UI CCWGG 4 cut(s) 11, 591, 723, 1071
Bst4CI ACNGT 3 cut(s) 305, 1107, 1160
Bst6I CTCTTC 1 cut(s) 277
BstAPI GCANNNNNTGC 1 cut(s) 1234
BstBI TTCGAA 2 cut(s) 216, 296
BstC8I GCNNGC 1 cut(s) 267
BstDSI CCRYGG 1 cut(s) 595
BstENI CCTNNNNNAGG 1 cut(s) 749
BstF5I GGATG 2 cut(s) 50, 469
BstFNI CGCG 2 cut(s) 695, 1038
BstHHI GCGC 1 cut(s) 695
BstKTI GATC 5 cut(s) 252, 495, 858, 933, 970
BstMAI GTCTC 2 cut(s) 519, 1008
BstMBI GATC 5 cut(s) 249, 492, 855, 930, 967
BstMCI CGRYCG 1 cut(s) 933
BstMWI GCNNNNNNNGC 3 cut(s) 591, 990, 1234
BstNI CCWGG 4 cut(s) 11, 591, 723, 1071
BstNSI RCATGY 2 cut(s) 150, 924
BstSCI CCNGG 5 cut(s) 9, 100, 589, 721, 1069
BstSFI CTRYAG 2 cut(s) 263, 1256
BstSLI GKGCMC 1 cut(s) 407
BstUI CGCG 2 cut(s) 695, 1038
BstX2I RGATCY 1 cut(s) 249
BstXI CCANNNNNNTGG 1 cut(s) 352
BstYI RGATCY 1 cut(s) 249
BsuRI GGCC 5 cut(s) 594, 712, 1024, 1146, 1171
BtgI CCRYGG 1 cut(s) 595
BtgZI GCGATG 1 cut(s) 423
BtsCI GGATG 2 cut(s) 50, 469
BtsI GCAGTG 1 cut(s) 260
BtsIMutI CAGTG 2 cut(s) 260, 1137
Cac8I GCNNGC 1 cut(s) 267
CciI TCATGA 1 cut(s) 660
CfoI GCGC 1 cut(s) 695
Cfr10I RCCGGY 1 cut(s) 1180
Cfr13I GGNCC 3 cut(s) 221, 587, 1145
CseI GACGC 2 cut(s) 27, 1027
Csp6I GTAC 2 cut(s) 4, 193
CspAI ACCGGT 1 cut(s) 1180
CviAII CATG 9 cut(s) 135, 147, 290, 352, 661, 708, 921, 939, 1186
CviQI GTAC 2 cut(s) 4, 193
DpnI GATC 5 cut(s) 251, 494, 857, 932, 969
DpnII GATC 5 cut(s) 249, 492, 855, 930, 967
DriI GACNNNNNGTC 1 cut(s) 1181
EaeI YGGCCR 1 cut(s) 1169
Eam1104I CTCTTC 1 cut(s) 277
Eam1105I GACNNNNNGTC 1 cut(s) 1181
EarI CTCTTC 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 253
Eco147I AGGCCT 1 cut(s) 1024
Eco32I GATATC 3 cut(s) 310, 319, 742
Eco47I GGWCC 2 cut(s) 221, 587
Eco88I CYCGRG 1 cut(s) 808
EcoNI CCTNNNNNAGG 1 cut(s) 749
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 4 cut(s) 9, 589, 721, 1069
EcoRV GATATC 3 cut(s) 310, 319, 742
EcoT14I CCWWGG 1 cut(s) 253
EcoT22I ATGCAT 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 253
Esp3I CGTCTC 2 cut(s) 519, 1008
FaeI CATG 9 cut(s) 138, 150, 293, 355, 664, 711, 924, 942, 1189
FalI AAGNNNNNCTT 2 cut(s) 981, 1013
FatI CATG 9 cut(s) 134, 146, 289, 351, 660, 707, 920, 938, 1185
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 2 cut(s) 1094, 1144
FokI GGATG 2 cut(s) 37, 476
Fsp4HI GCNGC 2 cut(s) 1094, 1144
FspBI CTAG 4 cut(s) 78, 368, 566, 1026
GlaI GCGC 1 cut(s) 694
GluI GCNGC 2 cut(s) 1094, 1144
HaeIII GGCC 5 cut(s) 594, 712, 1024, 1146, 1171
HapII CCGG 3 cut(s) 102, 522, 1181
HgaI GACGC 2 cut(s) 27, 1027
HhaI GCGC 1 cut(s) 695
Hin1II CATG 9 cut(s) 138, 150, 293, 355, 664, 711, 924, 942, 1189
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HinfI GANTC 5 cut(s) 158, 651, 716, 778, 1088
HpaII CCGG 3 cut(s) 102, 522, 1181
HphI GGTGA 5 cut(s) 317, 328, 778, 1097, 1116
Hpy166II GTNNAC 3 cut(s) 42, 405, 913
Hpy188III TCNNGA 4 cut(s) 661, 775, 810, 874
Hpy8I GTNNAC 3 cut(s) 42, 405, 913
Hpy99I CGWCG 3 cut(s) 578, 847, 1043
HpyAV CCTTC 3 cut(s) 212, 542, 983
HpyCH4III ACNGT 3 cut(s) 305, 1107, 1160
HpyCH4IV ACGT 2 cut(s) 6, 576
HpyF10VI GCNNNNNNNGC 3 cut(s) 591, 990, 1234
HpySE526I ACGT 2 cut(s) 6, 576
Hsp92II CATG 9 cut(s) 138, 150, 293, 355, 664, 711, 924, 942, 1189
HspAI GCGC 1 cut(s) 693
Kzo9I GATC 5 cut(s) 249, 492, 855, 930, 967
LguI GCTCTTC 1 cut(s) 277
LmnI GCTCC 3 cut(s) 238, 274, 567
LweI GCATC 4 cut(s) 133, 161, 442, 815
MaeI CTAG 4 cut(s) 78, 368, 566, 1026
MaeII ACGT 2 cut(s) 6, 576
MaeIII GTNAC 5 cut(s) 73, 499, 568, 577, 652
MalI GATC 5 cut(s) 251, 494, 857, 932, 969
MboI GATC 5 cut(s) 249, 492, 855, 930, 967
MboII GAAGA 2 cut(s) 294, 971
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 1 cut(s) 249
MhlI GDGCHC 3 cut(s) 27, 289, 407
MlsI TGGCCA 1 cut(s) 1171
MluCI AATT 3 cut(s) 340, 468, 903
MluNI TGGCCA 1 cut(s) 1171
MlyI GAGTC 2 cut(s) 167, 660
MmeI TCCRAC 2 cut(s) 825, 1021
Mox20I TGGCCA 1 cut(s) 1171
Mph1103I ATGCAT 1 cut(s) 148
MscI TGGCCA 1 cut(s) 1171
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 2 cut(s) 350, 659
Msp20I TGGCCA 1 cut(s) 1171
MspI CCGG 3 cut(s) 102, 522, 1181
MspR9I CCNGG 5 cut(s) 11, 102, 591, 723, 1071
MunI CAATTG 1 cut(s) 903
MvaI CCWGG 4 cut(s) 11, 591, 723, 1071
MvnI CGCG 2 cut(s) 695, 1038
MwoI GCNNNNNNNGC 3 cut(s) 591, 990, 1234
NciI CCSGG 1 cut(s) 102
NdeII GATC 5 cut(s) 249, 492, 855, 930, 967
NlaIII CATG 9 cut(s) 138, 150, 293, 355, 664, 711, 924, 942, 1189
NlaIV GGNNCC 3 cut(s) 234, 270, 553
NmuCI GTSAC 4 cut(s) 73, 499, 568, 652
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 2 cut(s) 150, 924
NspV TTCGAA 2 cut(s) 216, 296
PagI TCATGA 1 cut(s) 660
PceI AGGCCT 1 cut(s) 1024
PciSI GCTCTTC 1 cut(s) 277
PfeI GAWTC 3 cut(s) 716, 778, 1088
PflMI CCANNNNNTGG 1 cut(s) 949
PinAI ACCGGT 1 cut(s) 1180
PkrI GCNGC 2 cut(s) 1095, 1145
Ple19I CGATCG 1 cut(s) 933
PleI GAGTC 2 cut(s) 166, 659
PpsI GAGTC 2 cut(s) 166, 659
PpuMI RGGWCCY 1 cut(s) 221
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 4 cut(s) 9, 589, 721, 1069
PspGI CCWGG 4 cut(s) 9, 589, 721, 1069
PspN4I GGNNCC 3 cut(s) 234, 270, 553
PspPI GGNCC 3 cut(s) 221, 587, 1145
PspPPI RGGWCCY 1 cut(s) 221
PstI CTGCAG 1 cut(s) 267
PsuI RGATCY 1 cut(s) 249
PvuI CGATCG 1 cut(s) 933
RsaI GTAC 2 cut(s) 5, 194
RsaNI GTAC 2 cut(s) 4, 193
RseI CAYNNNNRTG 2 cut(s) 350, 659
SapI GCTCTTC 1 cut(s) 277
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 2 cut(s) 1094, 1144
Sau3AI GATC 5 cut(s) 249, 492, 855, 930, 967
Sau96I GGNCC 3 cut(s) 221, 587, 1145
SchI GAGTC 2 cut(s) 167, 660
ScrFI CCNGG 5 cut(s) 11, 102, 591, 723, 1071
SduI GDGCHC 3 cut(s) 27, 289, 407
SfaNI GCATC 4 cut(s) 133, 161, 442, 815
SfcI CTRYAG 2 cut(s) 263, 1256
SfuI TTCGAA 2 cut(s) 216, 296
SinI GGWCC 2 cut(s) 221, 587
SmiMI CAYNNNNRTG 2 cut(s) 350, 659
SpeI ACTAGT 1 cut(s) 77
Sse9I AATT 3 cut(s) 340, 468, 903
SseBI AGGCCT 1 cut(s) 1024
SsiI CCGC 4 cut(s) 186, 585, 1094, 1143
SspI AATATT 2 cut(s) 412, 514
SspMI CTAG 4 cut(s) 78, 368, 566, 1026
StuI AGGCCT 1 cut(s) 1024
StyD4I CCNGG 5 cut(s) 9, 100, 589, 721, 1069
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 3 cut(s) 305, 1107, 1160
TaiI ACGT 2 cut(s) 9, 579
TaqI TCGA 4 cut(s) 216, 296, 420, 774
TaqII GACCGA 2 cut(s) 294, 587
TasI AATT 3 cut(s) 340, 468, 903
TauI GCSGC 2 cut(s) 1096, 1146
TfiI GAWTC 3 cut(s) 716, 778, 1088
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 2 cut(s) 267, 1144
TseFI GTSAC 4 cut(s) 73, 499, 568, 652
Tsp45I GTSAC 4 cut(s) 73, 499, 568, 652
TspDTI ATGAA 2 cut(s) 648, 677
TspGWI ACGGA 2 cut(s) 476, 831
TspRI CASTG 2 cut(s) 267, 1144
Van91I CCANNNNNTGG 1 cut(s) 949
VneI GTGCAC 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 221, 587
XagI CCTNNNNNAGG 1 cut(s) 749
XceI RCATGY 2 cut(s) 150, 924
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 4 cut(s) 78, 368, 566, 1026
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.