Rh5BG513100

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0013649
Physical Location & Seq
Forward (+)
81407319 .. 81408781
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG513100.1

Sequence Viewer

Length: 1353 bp
ATGGGTCTGAAATTGGTGAATGTCCTGGCAATACTTGTGCTCTCATTGTTAGCGTCTACAACTACATCCCAAGCCGATGTGTTTGATGTGACTAGTTCAATCTATGGTGGAAAGCCCGGCTCTGATATTACAGAGGGTTTGGCAAAGGCATGGAGAGATGCATGTGCATCAGAGTCGTCAAGTAAGGTTGTTGTTCCAAGCGGTGCGTACAAGTTGAAAGAAGCAACATTCGAAGGTCCTTGCAAGGCTCCGATTGAGGTTAAGATCCAAGGAACACTGCAGGCTCCAAAAGACCGAAGTTCAGATACTTGGGTTGCATTTAGCTCCATTGACATGCTCACCTTATGGGGTGGTGGAACTTTTGACGGCCAAGGAGCACATGGTTCGAAAGACGGTGATATCAGAGATATCAGGTTCTCCTCCATCACCAATTCCATAATTATGGACATAACTTCACTAGATAGTAAGAAATACCACATAAGTATTTCCGAGTGCACCAATATTGTGTTCGACCACCTTACCATTACATCGCCTACAAACGATGCTAACACGGATGGAATTGACATTGGGAGTTCAACAAGGATCAATGTCACTCACACAGATATTGCTACCGGAGACGATTGTATTGCCGTTGGAGAAGGCACCAACCAGCTCCTAGTGACCGACGTTACTTGCGGACCAGGCCACGGCATAAGCATAGGAAGTCTTGGATATAAGCCTGATGAAAAGCCCGTGTTTGGAGTCACAATCATGAACTGCACCCTTGTTGGCACAACCAATGGCGCGAGAATAAAGACATGGCCTGATTCTCCTGGTGTAAGTAGTGCCTCGGATATCCTCTTTGAGGATATTGTAATGGTGAATGTCGAGAATCCTATCATCATTGACCAAAACTACTGCTCGGGAGATGAGGATAGATGCAAACAGAATACTCCGTCGGAAGTTCAGATCAGTAATGTGAGGTTCAAGAACATCAAAGGCTCGTCTGCAACTCCAATTGCTGTAAACCTTGCATGTAGTCCGATCGTGCCATGCCAGAATGTGGAACTGGAAGATATTGATCTTACATACAATGGCAACGAAGGCTCTCTTTCGTCTCAATGTTTCAATGTCAGGCCTAGAACCACTCGCGTCGGAAAACCTCTTGCTTGTGCTAATCTACCAGGCTTACCATCGGGTGATTCGCCGCCTCCACCACCGTTATATGATTACCATTCACCTATGATATACACTGGCGGCCCAGACAATAACAGTTGTTGGTGGCCAAGACCACCGGTCATGGGGTATCTACTAATTTCGGGTCTGGGTGTGTGGCTGTTGCAAGAGTTGCTACAAGAATACAGCAAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

450

Amino Acids

48.0

Weight (kDa)

4.7

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 372 5.3e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 641
AccB7I CCANNNNNTGG 1 cut(s) 1042
AccI GTMKAC 1 cut(s) 56
AccII CGCG 2 cut(s) 785, 1131
AciI CCGC 4 cut(s) 201, 675, 1187, 1236
AclWI GGATC 2 cut(s) 259, 590
AcoI YGGCCR 2 cut(s) 367, 1262
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 5 cut(s) 686, 737, 844, 1042, 1280
AgeI ACCGGT 1 cut(s) 1273
AgsI TTSAA 5 cut(s) 99, 217, 576, 967, 1108
AhdI GACNNNNNGTC 1 cut(s) 1274
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 4 cut(s) 24, 679, 811, 1162
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AluBI AGCT 2 cut(s) 324, 652
AluI AGCT 2 cut(s) 324, 652
Alw21I GWGCWC 3 cut(s) 42, 379, 497
Alw26I GTCTC 2 cut(s) 609, 1101
Alw44I GTGCAC 1 cut(s) 493
AlwI GGATC 2 cut(s) 259, 590
Ama87I CYCGRG 1 cut(s) 901
AoxI GGCC 6 cut(s) 367, 682, 800, 1115, 1237, 1262
ApaLI GTGCAC 1 cut(s) 493
AsiGI ACCGGT 1 cut(s) 1273
AspLEI GCGC 1 cut(s) 785
AspS9I GGNCC 3 cut(s) 236, 677, 1238
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 7 cut(s) 28, 331, 407, 418, 871, 1190, 1209
AsuII TTCGAA 2 cut(s) 231, 386
AvaI CYCGRG 1 cut(s) 901
AvaII GGWCC 2 cut(s) 236, 677
BaeGI GKGCMC 1 cut(s) 497
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BalI TGGCCA 1 cut(s) 1264
BanI GGYRCC 1 cut(s) 641
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
Bbv12I GWGCWC 3 cut(s) 42, 379, 497
BccI CCATC 3 cut(s) 431, 548, 1180
BceAI ACGGC 3 cut(s) 382, 614, 703
BcgI CGANNNNNNTGC 6 cut(s) 156, 190, 366, 400, 608, 642
BciT130I CCWGG 4 cut(s) 26, 681, 813, 1164
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 2 cut(s) 609, 1101
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 4 cut(s) 93, 458, 656, 1119
BfmI CTRYAG 2 cut(s) 278, 1349
BisI GCNGC 2 cut(s) 1187, 1237
BlsI GCNGC 2 cut(s) 1188, 1238
Bme1390I CCNGG 5 cut(s) 26, 117, 681, 813, 1164
Bme18I GGWCC 2 cut(s) 236, 677
BmeRI GACNNNNNGTC 1 cut(s) 1274
BmeT110I CYCGRG 1 cut(s) 901
BmgT120I GGNCC 3 cut(s) 236, 677, 1238
BmiI GGNNCC 3 cut(s) 249, 285, 643
BmrFI CCNGG 5 cut(s) 26, 117, 681, 813, 1164
BmsI GCATC 4 cut(s) 148, 176, 532, 908
Bpu14I TTCGAA 2 cut(s) 231, 386
BpuMI CCSGG 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 1059
BsaJI CCNNGG 4 cut(s) 268, 370, 685, 828
BsaWI WCCGGW 2 cut(s) 611, 1273
Bsc4I CCNNNNNNNGG 5 cut(s) 686, 737, 844, 1042, 1280
Bse118I RCCGGY 1 cut(s) 1273
Bse1I ACTGG 2 cut(s) 1053, 1237
Bse8I GATNNNNATC 1 cut(s) 1059
BseBI CCWGG 4 cut(s) 26, 681, 813, 1164
BseDI CCNNGG 4 cut(s) 268, 370, 685, 828
BseGI GGATG 2 cut(s) 65, 559
BseJI GATNNNNATC 1 cut(s) 1059
BseLI CCNNNNNNNGG 5 cut(s) 686, 737, 844, 1042, 1280
BseNI ACTGG 2 cut(s) 1053, 1237
BseRI GAGGAG 1 cut(s) 409
BseSI GKGCMC 1 cut(s) 497
BsgI GTGCAG 1 cut(s) 742
Bsh1236I CGCG 2 cut(s) 785, 1131
Bsh1285I CGRYCG 1 cut(s) 1026
BshFI GGCC 6 cut(s) 369, 684, 802, 1117, 1239, 1264
BshNI GGYRCC 1 cut(s) 641
BshTI ACCGGT 1 cut(s) 1273
BsiEI CGRYCG 1 cut(s) 1026
BsiHKAI GWGCWC 3 cut(s) 42, 379, 497
BsiHKCI CYCGRG 1 cut(s) 901
BsiSI CCGG 3 cut(s) 117, 612, 1274
BslI CCNNNNNNNGG 5 cut(s) 686, 737, 844, 1042, 1280
BsmAI GTCTC 2 cut(s) 609, 1101
BsmBI CGTCTC 2 cut(s) 609, 1101
BsnI GGCC 6 cut(s) 369, 684, 802, 1117, 1239, 1264
BsoBI CYCGRG 1 cut(s) 901
Bsp119I TTCGAA 2 cut(s) 231, 386
Bsp1286I GDGCHC 3 cut(s) 42, 379, 497
Bsp143I GATC 5 cut(s) 264, 582, 948, 1023, 1060
BspACI CCGC 4 cut(s) 201, 675, 1187, 1236
BspANI GGCC 6 cut(s) 369, 684, 802, 1117, 1239, 1264
BspFNI CGCG 2 cut(s) 785, 1131
BspHI TCATGA 1 cut(s) 750
BspLI GGNNCC 3 cut(s) 249, 285, 643
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 2 cut(s) 259, 590
BspT104I TTCGAA 2 cut(s) 231, 386
BspT107I GGYRCC 1 cut(s) 641
BsrFI RCCGGY 1 cut(s) 1273
BsrI ACTGG 2 cut(s) 1053, 1237
BssAI RCCGGY 1 cut(s) 1273
BssECI CCNNGG 4 cut(s) 268, 370, 685, 828
BssMI GATC 5 cut(s) 264, 582, 948, 1023, 1060
BssT1I CCWWGG 2 cut(s) 268, 370
Bst2UI CCWGG 4 cut(s) 26, 681, 813, 1164
Bst4CI ACNGT 3 cut(s) 395, 1200, 1253
BstAPI GCANNNNNTGC 1 cut(s) 1327
BstBI TTCGAA 2 cut(s) 231, 386
BstC8I GCNNGC 1 cut(s) 282
BstDSI CCRYGG 1 cut(s) 685
BstENI CCTNNNNNAGG 1 cut(s) 842
BstF5I GGATG 2 cut(s) 65, 559
BstFNI CGCG 2 cut(s) 785, 1131
BstHHI GCGC 1 cut(s) 785
BstKTI GATC 5 cut(s) 267, 585, 951, 1026, 1063
BstMAI GTCTC 2 cut(s) 609, 1101
BstMBI GATC 5 cut(s) 264, 582, 948, 1023, 1060
BstMCI CGRYCG 1 cut(s) 1026
BstMWI GCNNNNNNNGC 3 cut(s) 681, 1083, 1327
BstNI CCWGG 4 cut(s) 26, 681, 813, 1164
BstNSI RCATGY 3 cut(s) 165, 337, 1017
BstSCI CCNGG 5 cut(s) 24, 115, 679, 811, 1162
BstSFI CTRYAG 2 cut(s) 278, 1349
BstSLI GKGCMC 1 cut(s) 497
BstUI CGCG 2 cut(s) 785, 1131
BstX2I RGATCY 1 cut(s) 264
BstXI CCANNNNNNTGG 1 cut(s) 442
BstYI RGATCY 1 cut(s) 264
BsuRI GGCC 6 cut(s) 369, 684, 802, 1117, 1239, 1264
BtgI CCRYGG 1 cut(s) 685
BtgZI GCGATG 1 cut(s) 513
BtsCI GGATG 2 cut(s) 65, 559
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 2 cut(s) 275, 1230
Cac8I GCNNGC 1 cut(s) 282
CciI TCATGA 1 cut(s) 750
CfoI GCGC 1 cut(s) 785
Cfr10I RCCGGY 1 cut(s) 1273
Cfr13I GGNCC 3 cut(s) 236, 677, 1238
CseI GACGC 2 cut(s) 42, 1120
Csp6I GTAC 1 cut(s) 208
CspAI ACCGGT 1 cut(s) 1273
CviAII CATG 9 cut(s) 150, 162, 334, 380, 751, 798, 1014, 1032, 1279
CviQI GTAC 1 cut(s) 208
DpnI GATC 5 cut(s) 266, 584, 950, 1025, 1062
DpnII GATC 5 cut(s) 264, 582, 948, 1023, 1060
DriI GACNNNNNGTC 1 cut(s) 1274
EaeI YGGCCR 2 cut(s) 367, 1262
Eam1105I GACNNNNNGTC 1 cut(s) 1274
Eco130I CCWWGG 2 cut(s) 268, 370
Eco147I AGGCCT 1 cut(s) 1117
Eco32I GATATC 3 cut(s) 400, 409, 835
Eco47I GGWCC 2 cut(s) 236, 677
Eco88I CYCGRG 1 cut(s) 901
EcoNI CCTNNNNNAGG 1 cut(s) 842
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 4 cut(s) 24, 679, 811, 1162
EcoRV GATATC 3 cut(s) 400, 409, 835
EcoT14I CCWWGG 2 cut(s) 268, 370
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 268, 370
Esp3I CGTCTC 2 cut(s) 609, 1101
FaeI CATG 9 cut(s) 153, 165, 337, 383, 754, 801, 1017, 1035, 1282
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FatI CATG 9 cut(s) 149, 161, 333, 379, 750, 797, 1013, 1031, 1278
FblI GTMKAC 1 cut(s) 56
Fnu4HI GCNGC 2 cut(s) 1187, 1237
FokI GGATG 2 cut(s) 52, 566
Fsp4HI GCNGC 2 cut(s) 1187, 1237
FspBI CTAG 4 cut(s) 93, 458, 656, 1119
GlaI GCGC 1 cut(s) 784
GluI GCNGC 2 cut(s) 1187, 1237
HaeIII GGCC 6 cut(s) 369, 684, 802, 1117, 1239, 1264
HapII CCGG 3 cut(s) 117, 612, 1274
HgaI GACGC 2 cut(s) 42, 1120
HhaI GCGC 1 cut(s) 785
Hin1II CATG 9 cut(s) 153, 165, 337, 383, 754, 801, 1017, 1035, 1282
Hin6I GCGC 1 cut(s) 783
HinP1I GCGC 1 cut(s) 783
HinfI GANTC 5 cut(s) 173, 741, 806, 871, 1181
HpaII CCGG 3 cut(s) 117, 612, 1274
HphI GGTGA 7 cut(s) 28, 331, 407, 418, 871, 1190, 1209
Hpy166II GTNNAC 3 cut(s) 57, 495, 1006
Hpy188III TCNNGA 4 cut(s) 751, 868, 903, 967
Hpy8I GTNNAC 3 cut(s) 57, 495, 1006
Hpy99I CGWCG 3 cut(s) 668, 940, 1136
HpyAV CCTTC 3 cut(s) 227, 632, 1076
HpyCH4III ACNGT 3 cut(s) 395, 1200, 1253
HpyCH4IV ACGT 1 cut(s) 666
HpyF10VI GCNNNNNNNGC 3 cut(s) 681, 1083, 1327
HpySE526I ACGT 1 cut(s) 666
Hsp92II CATG 9 cut(s) 153, 165, 337, 383, 754, 801, 1017, 1035, 1282
HspAI GCGC 1 cut(s) 783
Kzo9I GATC 5 cut(s) 264, 582, 948, 1023, 1060
LmnI GCTCC 5 cut(s) 253, 289, 329, 374, 657
LweI GCATC 4 cut(s) 148, 176, 532, 908
MaeI CTAG 4 cut(s) 93, 458, 656, 1119
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 5 cut(s) 88, 589, 658, 667, 742
MalI GATC 5 cut(s) 266, 584, 950, 1025, 1062
MboI GATC 5 cut(s) 264, 582, 948, 1023, 1060
MboII GAAGA 1 cut(s) 1064
MfeI CAATTG 1 cut(s) 996
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 3 cut(s) 42, 379, 497
MlsI TGGCCA 1 cut(s) 1264
MluCI AATT 6 cut(s) 11, 430, 438, 558, 996, 1293
MluNI TGGCCA 1 cut(s) 1264
MlyI GAGTC 2 cut(s) 182, 750
MmeI TCCRAC 3 cut(s) 613, 918, 1114
Mox20I TGGCCA 1 cut(s) 1264
Mph1103I ATGCAT 1 cut(s) 163
MscI TGGCCA 1 cut(s) 1264
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 3 cut(s) 332, 440, 749
Msp20I TGGCCA 1 cut(s) 1264
MspI CCGG 3 cut(s) 117, 612, 1274
MspR9I CCNGG 5 cut(s) 26, 117, 681, 813, 1164
MunI CAATTG 1 cut(s) 996
MvaI CCWGG 4 cut(s) 26, 681, 813, 1164
MvnI CGCG 2 cut(s) 785, 1131
MwoI GCNNNNNNNGC 3 cut(s) 681, 1083, 1327
NciI CCSGG 1 cut(s) 117
NdeII GATC 5 cut(s) 264, 582, 948, 1023, 1060
NlaIII CATG 9 cut(s) 153, 165, 337, 383, 754, 801, 1017, 1035, 1282
NlaIV GGNNCC 3 cut(s) 249, 285, 643
NmuCI GTSAC 4 cut(s) 88, 589, 658, 742
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 3 cut(s) 165, 337, 1017
NspV TTCGAA 2 cut(s) 231, 386
PagI TCATGA 1 cut(s) 750
PceI AGGCCT 1 cut(s) 1117
PfeI GAWTC 3 cut(s) 806, 871, 1181
PflMI CCANNNNNTGG 1 cut(s) 1042
PinAI ACCGGT 1 cut(s) 1273
PkrI GCNGC 2 cut(s) 1188, 1238
Ple19I CGATCG 1 cut(s) 1026
PleI GAGTC 2 cut(s) 181, 749
PpsI GAGTC 2 cut(s) 181, 749
PpuMI RGGWCCY 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 4 cut(s) 24, 679, 811, 1162
PspGI CCWGG 4 cut(s) 24, 679, 811, 1162
PspN4I GGNNCC 3 cut(s) 249, 285, 643
PspPI GGNCC 3 cut(s) 236, 677, 1238
PspPPI RGGWCCY 1 cut(s) 236
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 264
PvuI CGATCG 1 cut(s) 1026
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 3 cut(s) 332, 440, 749
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 2 cut(s) 1187, 1237
Sau3AI GATC 5 cut(s) 264, 582, 948, 1023, 1060
Sau96I GGNCC 3 cut(s) 236, 677, 1238
SchI GAGTC 2 cut(s) 182, 750
ScrFI CCNGG 5 cut(s) 26, 117, 681, 813, 1164
SduI GDGCHC 3 cut(s) 42, 379, 497
SfaNI GCATC 4 cut(s) 148, 176, 532, 908
SfcI CTRYAG 2 cut(s) 278, 1349
SfuI TTCGAA 2 cut(s) 231, 386
SinI GGWCC 2 cut(s) 236, 677
SmiMI CAYNNNNRTG 3 cut(s) 332, 440, 749
SpeI ACTAGT 1 cut(s) 92
Sse9I AATT 6 cut(s) 11, 430, 438, 558, 996, 1293
SseBI AGGCCT 1 cut(s) 1117
SsiI CCGC 4 cut(s) 201, 675, 1187, 1236
SspI AATATT 1 cut(s) 502
SspMI CTAG 4 cut(s) 93, 458, 656, 1119
StuI AGGCCT 1 cut(s) 1117
StyD4I CCNGG 5 cut(s) 24, 115, 679, 811, 1162
StyI CCWWGG 2 cut(s) 268, 370
TaaI ACNGT 3 cut(s) 395, 1200, 1253
TaiI ACGT 1 cut(s) 669
TaqI TCGA 4 cut(s) 231, 386, 510, 867
TaqII GACCGA 2 cut(s) 309, 677
TasI AATT 6 cut(s) 11, 430, 438, 558, 996, 1293
TauI GCSGC 2 cut(s) 1189, 1239
TfiI GAWTC 3 cut(s) 806, 871, 1181
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 282, 1237
TseFI GTSAC 4 cut(s) 88, 589, 658, 742
Tsp45I GTSAC 4 cut(s) 88, 589, 658, 742
TspDTI ATGAA 2 cut(s) 738, 767
TspGWI ACGGA 2 cut(s) 566, 924
TspRI CASTG 2 cut(s) 282, 1237
Van91I CCANNNNNTGG 1 cut(s) 1042
VneI GTGCAC 1 cut(s) 493
VpaK11BI GGWCC 2 cut(s) 236, 677
XagI CCTNNNNNAGG 1 cut(s) 842
XceI RCATGY 3 cut(s) 165, 337, 1017
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 56
XspI CTAG 4 cut(s) 93, 458, 656, 1119
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.