FvH4_3g42180

RecX family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009626
Physical Location & Seq
Forward (+)
35234377 .. 35237523
3147 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42180.t1

Sequence Viewer

Length: 990 bp
ATGGCAATTTACGCGGGAACCCTCTGCTTCAAAACCCAATTGCAGCTTCACTGTCGAGTTTTATTCATGCCCTGGGTTAGGAAGAACAGTGCAATTTTTTGCTTGAAAAGCAGAGACTACAGTTCATCTGTTCCGGCTAGGTACATTCCGAAGAGAGTTTTTGAAGTTAACAAATCGGAAACTTCTCCGCAGAGAAAGCGTTCTGATAAAAAGGAATTTCGTGAGCTTTCGGATGGTTTTGCAATTAAGAGTGAGGTTAAACACCCGAGAAGTGGTGTACAGAACTATAGGGACTTTGATAAGAAGCTTCCCTCTAGTAACTCATTGCCTAATGACGTTGAGCAAGCATCTCCAGAGCTGGAGGATAAGATGGATCATCTATCAGGGGTTGCAGGCGATGATGAACTCATTGAAGAGCCTGAGGAGGTCTCTGAAGAGTTGAATGTATTACAAAATAGTAAAACTATGCATGAAGTTGAGAAAGCAGCAATAGAATTACTTGCTGGAAGGGCTTTCACTGCAGTTGAATTGAGAAAGAAATTATTTCGAAAGGGATTTTCTCTTGACGCCATTGAAGCAGTGATAAAAGATTTCATGAGAAGAGGGCTAATCAATGATAGCTTGTATGCGGAAGCATTTTCACGTTCTCGATGGTCTTCTTCAAGCTGGGGACCCAGGCGGATAAAGCAAGCACTTTTCACCAAGGGGGTTAGTGCAGTTGATGCTGAGAAGGCAATTAAACTGGTGTTTGAGGACAGAGACTCAGATGAGGATGGAGAATCAGATGATGGTCAGGAGTTAATTCATGGCATGTCAAAGATCTCAATGGACCATTTATTTGTTCAGGCCTCGAAACAATGGCTCCGAGGTGGAGAAGTTCCTAGAGAGATGAGGAAATCAAGGATTGTTCGTTGGCTTCAATATCGTGGTTTCAACTGGGGTGTTATCAGCTTTATATTAAAGAAGTTAGAATCTCAGCACCCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RecX

Protein Analysis

330

Amino Acids

37.75

Weight (kDa)

8.77

Isoelectric Point (pI)

56.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RecX_HTH1 PF21982 162 - 196 2.1e-07 RecX, first three-helix domain
RecX_HTH2 PF02631 205 - 245 1.7e-12 RecX, second three-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 14
AciI CCGC 4 cut(s) 14, 188, 629, 679
AclWI GGATC 1 cut(s) 381
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 567
AfaI GTAC 2 cut(s) 143, 279
AfiI CCNNNNNNNGG 2 cut(s) 78, 272
AjnI CCWGG 2 cut(s) 71, 674
AjuI GAANNNNNNNTTGG 2 cut(s) 30, 62
AluBI AGCT 7 cut(s) 46, 226, 307, 358, 621, 666, 951
AluI AGCT 7 cut(s) 46, 226, 307, 358, 621, 666, 951
Alw26I GTCTC 3 cut(s) 108, 433, 753
AlwI GGATC 1 cut(s) 381
Ama87I CYCGRG 1 cut(s) 265
AoxI GGCC 1 cut(s) 846
ApeKI GCWGC 2 cut(s) 43, 485
ApoI RAATTY 1 cut(s) 215
ArsI GACNNNNNNTTYG 2 cut(s) 821, 853
Asp700I GAANNNNTTC 1 cut(s) 199
AspS9I GGNCC 2 cut(s) 671, 829
AsuHPI GGTGA 1 cut(s) 691
AsuII TTCGAA 1 cut(s) 547
AvaI CYCGRG 1 cut(s) 265
AvaII GGWCC 2 cut(s) 671, 829
AxyI CCTNAGG 1 cut(s) 420
BbsI GAAGAC 1 cut(s) 648
BbvI GCAGC 2 cut(s) 55, 497
BccI CCATC 5 cut(s) 227, 364, 645, 767, 782
BciT130I CCWGG 2 cut(s) 73, 676
BcoDI GTCTC 3 cut(s) 108, 433, 753
BfaI CTAG 4 cut(s) 138, 315, 882, 988
BfmI CTRYAG 3 cut(s) 118, 286, 519
BglII AGATCT 1 cut(s) 819
BisI GCNGC 2 cut(s) 44, 486
BlsI GCNGC 2 cut(s) 45, 487
Bme1390I CCNGG 2 cut(s) 73, 676
Bme18I GGWCC 2 cut(s) 671, 829
BmeT110I CYCGRG 1 cut(s) 265
BmgT120I GGNCC 2 cut(s) 671, 829
BmiI GGNNCC 4 cut(s) 19, 672, 673, 863
BmrFI CCNGG 2 cut(s) 73, 676
BmrI ACTGGG 1 cut(s) 946
BmsI GCATC 2 cut(s) 356, 712
BmuI ACTGGG 1 cut(s) 946
BpiI GAAGAC 1 cut(s) 648
BplI GAGNNNNNCTC 2 cut(s) 413, 445
BpmI CTGGAG 2 cut(s) 336, 380
Bpu14I TTCGAA 1 cut(s) 547
BsaHI GRCGYC 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 433
BsaJI CCNNGG 5 cut(s) 71, 72, 674, 702, 865
BsaXI ACNNNNNCTCC 2 cut(s) 846, 876
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 272
Bse1I ACTGG 2 cut(s) 747, 941
Bse21I CCTNAGG 1 cut(s) 420
Bse3DI GCAATG 1 cut(s) 323
BseBI CCWGG 2 cut(s) 73, 676
BseDI CCNNGG 5 cut(s) 71, 72, 674, 702, 865
BseGI GGATG 2 cut(s) 238, 778
BseLI CCNNNNNNNGG 2 cut(s) 78, 272
BseMI GCAATG 1 cut(s) 323
BseMII CTCAG 4 cut(s) 411, 717, 777, 989
BseNI ACTGG 2 cut(s) 747, 941
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 2 cut(s) 55, 497
BseYI CCCAGC 1 cut(s) 666
BsgI GTGCAG 1 cut(s) 735
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 848
BsiHKCI CYCGRG 1 cut(s) 265
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 2 cut(s) 305, 684
BslI CCNNNNNNNGG 2 cut(s) 78, 272
BsmAI GTCTC 3 cut(s) 108, 433, 753
BsmFI GGGAC 2 cut(s) 305, 684
BsnI GGCC 1 cut(s) 848
Bso31I GGTCTC 1 cut(s) 433
BsoBI CYCGRG 1 cut(s) 265
Bsp119I TTCGAA 1 cut(s) 547
Bsp1407I TGTACA 1 cut(s) 277
Bsp143I GATC 2 cut(s) 373, 819
BspACI CCGC 4 cut(s) 14, 188, 629, 679
BspANI GGCC 1 cut(s) 848
BspCNI CTCAG 4 cut(s) 412, 718, 776, 988
BspFNI CGCG 1 cut(s) 14
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 4 cut(s) 19, 672, 673, 863
BspMAI CTGCAG 1 cut(s) 523
BspPI GGATC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 408
BspT104I TTCGAA 1 cut(s) 547
BspTNI GGTCTC 1 cut(s) 433
BsrDI GCAATG 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 277
BsrI ACTGG 2 cut(s) 747, 941
BssECI CCNNGG 5 cut(s) 71, 72, 674, 702, 865
BssMI GATC 2 cut(s) 373, 819
BssNI GRCGYC 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 702
Bst2UI CCWGG 2 cut(s) 73, 676
Bst4CI ACNGT 3 cut(s) 53, 89, 122
Bst6I CTCTTC 4 cut(s) 146, 408, 429, 595
BstACI GRCGYC 1 cut(s) 567
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 277
BstBI TTCGAA 1 cut(s) 547
BstC8I GCNNGC 3 cut(s) 345, 394, 690
BstDEI CTNAG 4 cut(s) 420, 726, 763, 975
BstENI CCTNNNNNAGG 1 cut(s) 76
BstF5I GGATG 2 cut(s) 238, 778
BstFNI CGCG 1 cut(s) 14
BstKTI GATC 2 cut(s) 376, 822
BstMAI GTCTC 3 cut(s) 108, 433, 753
BstMBI GATC 2 cut(s) 373, 819
BstMWI GCNNNNNNNGC 9 cut(s) 11, 108, 196, 509, 518, 575, 685, 722, 731
BstNI CCWGG 2 cut(s) 73, 676
BstNSI RCATGY 1 cut(s) 814
BstSCI CCNGG 2 cut(s) 71, 674
BstSFI CTRYAG 3 cut(s) 118, 286, 519
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 55, 497
BstV2I GAAGAC 1 cut(s) 648
BstX2I RGATCY 1 cut(s) 819
BstYI RGATCY 1 cut(s) 819
Bsu36I CCTNAGG 1 cut(s) 420
BsuRI GGCC 1 cut(s) 848
BtgZI GCGATG 1 cut(s) 411
BtsCI GGATG 2 cut(s) 238, 778
BtsI GCAGTG 2 cut(s) 516, 585
BtsIMutI CAGTG 4 cut(s) 49, 94, 516, 585
Cac8I GCNNGC 3 cut(s) 345, 394, 690
CciI TCATGA 1 cut(s) 594
Cfr13I GGNCC 2 cut(s) 671, 829
CseI GACGC 1 cut(s) 575
Csp6I GTAC 2 cut(s) 142, 278
CviAII CATG 5 cut(s) 67, 470, 595, 806, 811
CviQI GTAC 2 cut(s) 142, 278
DdeI CTNAG 4 cut(s) 420, 726, 763, 975
DpnI GATC 2 cut(s) 375, 821
DpnII GATC 2 cut(s) 373, 819
Eam1104I CTCTTC 4 cut(s) 146, 408, 429, 595
EarI CTCTTC 4 cut(s) 146, 408, 429, 595
EciI GGCGGA 1 cut(s) 694
Eco130I CCWWGG 1 cut(s) 702
Eco147I AGGCCT 1 cut(s) 848
Eco31I GGTCTC 1 cut(s) 433
Eco47I GGWCC 2 cut(s) 671, 829
Eco57I CTGAAG 1 cut(s) 453
Eco81I CCTNAGG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 76
EcoO109I RGGNCCY 1 cut(s) 671
EcoRII CCWGG 2 cut(s) 71, 674
EcoT14I CCWWGG 1 cut(s) 702
EcoT22I ATGCAT 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 702
FaeI CATG 5 cut(s) 70, 473, 598, 809, 814
FaiI YATR 9 cut(s) 68, 288, 467, 471, 596, 627, 807, 812, 956
FaqI GGGAC 2 cut(s) 305, 684
FatI CATG 5 cut(s) 66, 469, 594, 805, 810
FauI CCCGC 1 cut(s) 7
Fnu4HI GCNGC 2 cut(s) 44, 486
FokI GGATG 2 cut(s) 245, 785
Fsp4HI GCNGC 2 cut(s) 44, 486
FspBI CTAG 4 cut(s) 138, 315, 882, 988
GluI GCNGC 2 cut(s) 44, 486
GsaI CCCAGC 1 cut(s) 670
GsuI CTGGAG 2 cut(s) 336, 380
HaeIII GGCC 1 cut(s) 848
HapII CCGG 1 cut(s) 134
HgaI GACGC 1 cut(s) 575
Hin1I GRCGYC 1 cut(s) 567
Hin1II CATG 5 cut(s) 70, 473, 598, 809, 814
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 3 cut(s) 761, 779, 971
HpaI GTTAAC 1 cut(s) 169
HpaII CCGG 1 cut(s) 134
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 2 cut(s) 169, 278
Hpy188I TCNGA 8 cut(s) 150, 178, 205, 232, 433, 766, 784, 866
Hpy188III TCNNGA 6 cut(s) 221, 353, 563, 595, 648, 794
Hpy8I GTNNAC 2 cut(s) 169, 278
HpyAV CCTTC 2 cut(s) 501, 724
HpyCH4III ACNGT 3 cut(s) 53, 89, 122
HpyCH4IV ACGT 2 cut(s) 336, 643
HpyCH4V TGCA 7 cut(s) 43, 92, 242, 392, 469, 521, 716
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 108, 196, 509, 518, 575, 685, 722, 731
HpyF3I CTNAG 4 cut(s) 420, 726, 763, 975
HpySE526I ACGT 2 cut(s) 336, 643
Hsp92I GRCGYC 1 cut(s) 567
Hsp92II CATG 5 cut(s) 70, 473, 598, 809, 814
KflI GGGWCCC 1 cut(s) 671
KspAI GTTAAC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 373, 819
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 1 cut(s) 867
Lsp1109I GCAGC 2 cut(s) 55, 497
LweI GCATC 2 cut(s) 356, 712
MaeI CTAG 4 cut(s) 138, 315, 882, 988
MaeII ACGT 2 cut(s) 336, 643
MaeIII GTNAC 1 cut(s) 317
MalI GATC 2 cut(s) 375, 821
MboI GATC 2 cut(s) 373, 819
MboII GAAGA 7 cut(s) 94, 163, 425, 446, 612, 648, 651
MfeI CAATTG 1 cut(s) 38
MflI RGATCY 1 cut(s) 819
MlyI GAGTC 1 cut(s) 755
Mph1103I ATGCAT 1 cut(s) 471
MroXI GAANNNNTTC 1 cut(s) 199
MseI TTAA 6 cut(s) 168, 246, 258, 738, 800, 959
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 2 cut(s) 73, 676
MunI CAATTG 1 cut(s) 38
MvaI CCWGG 2 cut(s) 73, 676
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 9 cut(s) 11, 108, 196, 509, 518, 575, 685, 722, 731
NdeII GATC 2 cut(s) 373, 819
NlaIII CATG 5 cut(s) 70, 473, 598, 809, 814
NlaIV GGNNCC 4 cut(s) 19, 672, 673, 863
NsiI ATGCAT 1 cut(s) 471
NspI RCATGY 1 cut(s) 814
NspV TTCGAA 1 cut(s) 547
PagI TCATGA 1 cut(s) 594
PasI CCCWGGG 1 cut(s) 72
PceI AGGCCT 1 cut(s) 848
PciSI GCTCTTC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 199
PfeI GAWTC 2 cut(s) 779, 971
PkrI GCNGC 2 cut(s) 45, 487
PleI GAGTC 1 cut(s) 755
PpsI GAGTC 1 cut(s) 755
PpuMI RGGWCCY 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 671
Psp6I CCWGG 2 cut(s) 71, 674
PspFI CCCAGC 1 cut(s) 666
PspGI CCWGG 2 cut(s) 71, 674
PspN4I GGNNCC 4 cut(s) 19, 672, 673, 863
PspPI GGNCC 2 cut(s) 671, 829
PspPPI RGGWCCY 1 cut(s) 671
PstI CTGCAG 1 cut(s) 523
PsuI RGATCY 1 cut(s) 819
RsaI GTAC 2 cut(s) 143, 279
RsaNI GTAC 2 cut(s) 142, 278
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 6 cut(s) 168, 246, 258, 738, 800, 959
SatI GCNGC 2 cut(s) 44, 486
Sau3AI GATC 2 cut(s) 373, 819
Sau96I GGNCC 2 cut(s) 671, 829
SchI GAGTC 1 cut(s) 755
ScrFI CCNGG 2 cut(s) 73, 676
SfaNI GCATC 2 cut(s) 356, 712
SfcI CTRYAG 3 cut(s) 118, 286, 519
SfuI TTCGAA 1 cut(s) 547
SinI GGWCC 2 cut(s) 671, 829
SseBI AGGCCT 1 cut(s) 848
SsiI CCGC 4 cut(s) 14, 188, 629, 679
SspMI CTAG 4 cut(s) 138, 315, 882, 988
StuI AGGCCT 1 cut(s) 848
StyD4I CCNGG 2 cut(s) 71, 674
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 3 cut(s) 53, 89, 122
TaiI ACGT 2 cut(s) 339, 646
TaqI TCGA 4 cut(s) 55, 547, 649, 851
TatI WGTACW 1 cut(s) 277
TfiI GAWTC 2 cut(s) 779, 971
Tru1I TTAA 6 cut(s) 168, 246, 258, 738, 800, 959
Tru9I TTAA 6 cut(s) 168, 246, 258, 738, 800, 959
TscAI CASTG 4 cut(s) 56, 94, 523, 585
TseI GCWGC 2 cut(s) 43, 485
TspDTI ATGAA 6 cut(s) 55, 114, 417, 486, 583, 794
TspRI CASTG 4 cut(s) 56, 94, 523, 585
VpaK11BI GGWCC 2 cut(s) 671, 829
XagI CCTNNNNNAGG 1 cut(s) 76
XapI RAATTY 1 cut(s) 215
XceI RCATGY 1 cut(s) 814
XmnI GAANNNNTTC 1 cut(s) 199
XspI CTAG 4 cut(s) 138, 315, 882, 988
Zsp2I ATGCAT 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.