Rroxscaffold_1G00006050

RecX family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009626
Physical Location & Seq
Reverse (-)
8084904 .. 8087973
3070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006050.1

Sequence Viewer

Length: 987 bp
ATGGCAATTTACGCGGGAACCCTCTGCTTCAAAACCCAATTGCAGCTTCACTGTCGGGTTTTCTTGATGCCCTGGGTTAGAAGGAATAGTGCAATTTCATGCTTGAAAAATAGAGATTACAGTTCATCAGTTCCGGCTAGGTACATTCCAAAGAAATCTTCTGAAGTTAACAAATCGGAAACTTCTTTGCCGGCAAAGGGTTCTGATAAAAAAGAATTTCGTGAGCTTTCGGATGGTTTTGCATTGAAGAGTGAGGTTAAATATCGGAGAAGTGTACAGAACTATAAGAACTTTGATGAGAAGCTTCCCTATAGTAACTCATTGTCTAATGACGTTGAGCAGGCATCTCCAGAGCTGGAGGATCAGATCAATCATCTATCGGGGGCTGAAGGCGATGATGTACTCATGGAAGAGCCTGAGGAGGTTTCTGAAGAGTTGAATGTATTGCGAAATAGTAAAACTACACATGAAGTTGAGAAAGCAGCAATAGAATTACTTGCCGGAAGGGCTTTCACCGCAGTTGAATTGAGAAAGAAACTATGTCGAAAGGGATTTTCTCTTGACACCATTGAGGCAGTGATAAAAGATTTCATGAGAAGAGGGCTAATCAATGATAGCATGTATGCGGAAGCATTTTCACGTTCTAGATGGTCTTCCTCAAGTTGGGGACCAAGGCGGATAAAGCAAGCACTTTTCACCAAGGGGGTTAGTGAAGTTGACGCTGAGAAGGCAATTAAACTGGTGTTTGAGGATAGAAAATCAGATGAGGATGGAGAATCAGATGAGGATCAGGAGTTAATTCATGGCATGTCAAAGCTCTCAATGGACCATTTATTTGTTCAGGCCTCGAAACAATGGCTCCGAGGCAGAGAAGTTCCTCAAGAGACAAGAAAATCAAGGATTGTTCGATGGCTTCAATATCGTGGTTTCAACTGGGGTGTTATTGGCTTTATATTAAAGAAGTTAGAATCTGAGCACCCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RecX

Protein Analysis

328

Amino Acids

37.67

Weight (kDa)

8.2

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RecX_HTH1 PF21982 161 - 195 1.9e-07 RecX, first three-helix domain
RecX_HTH2 PF02631 204 - 244 1.4e-12 RecX, second three-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 14
AciI CCGC 4 cut(s) 14, 516, 626, 676
AclWI GGATC 2 cut(s) 369, 795
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 3 cut(s) 183, 408, 450
AfaI GTAC 3 cut(s) 143, 276, 402
AfiI CCNNNNNNNGG 2 cut(s) 197, 663
AgsI TTSAA 7 cut(s) 31, 106, 247, 439, 524, 917, 931
AjnI CCWGG 1 cut(s) 71
AjuI GAANNNNNNNTTGG 4 cut(s) 30, 62, 142, 174
AluBI AGCT 5 cut(s) 46, 226, 304, 355, 817
AluI AGCT 5 cut(s) 46, 226, 304, 355, 817
Alw21I GWGCWC 1 cut(s) 978
Alw26I GTCTC 1 cut(s) 878
AlwI GGATC 2 cut(s) 369, 795
AoxI GGCC 1 cut(s) 843
ApeKI GCWGC 2 cut(s) 43, 482
ApoI RAATTY 1 cut(s) 215
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
AspS9I GGNCC 2 cut(s) 668, 826
AsuHPI GGTGA 2 cut(s) 505, 688
AvaII GGWCC 2 cut(s) 668, 826
AxyI CCTNAGG 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 645
Bbv12I GWGCWC 1 cut(s) 978
BbvI GCAGC 2 cut(s) 55, 494
BccI CCATC 4 cut(s) 227, 642, 764, 903
BciT130I CCWGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 878
BfaI CTAG 3 cut(s) 138, 645, 985
BfmI CTRYAG 1 cut(s) 310
BglI GCCNNNNNGGC 1 cut(s) 506
BisI GCNGC 2 cut(s) 44, 483
BlsI GCNGC 2 cut(s) 45, 484
Bme1390I CCNGG 1 cut(s) 73
Bme18I GGWCC 2 cut(s) 668, 826
BmgT120I GGNCC 2 cut(s) 668, 826
BmiI GGNNCC 3 cut(s) 19, 669, 860
BmrFI CCNGG 1 cut(s) 73
BmrI ACTGGG 1 cut(s) 943
BmsI GCATC 2 cut(s) 57, 353
BmuI ACTGGG 1 cut(s) 943
BpiI GAAGAC 1 cut(s) 645
BpmI CTGGAG 2 cut(s) 333, 377
BpuEI CTTGAG 2 cut(s) 643, 864
BsaBI GATNNNNATC 1 cut(s) 786
BsaJI CCNNGG 5 cut(s) 71, 72, 671, 699, 862
BsaXI ACNNNNNCTCC 2 cut(s) 843, 873
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 663
Bse118I RCCGGY 1 cut(s) 190
Bse1I ACTGG 2 cut(s) 744, 938
Bse21I CCTNAGG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 5 cut(s) 71, 72, 671, 699, 862
BseGI GGATG 2 cut(s) 238, 775
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 2 cut(s) 197, 663
BseMII CTCAG 3 cut(s) 408, 714, 963
BseNI ACTGG 2 cut(s) 744, 938
BseRI GAGGAG 1 cut(s) 434
BseXI GCAGC 2 cut(s) 55, 494
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 845
BsiHKAI GWGCWC 1 cut(s) 978
BsiSI CCGG 3 cut(s) 134, 191, 501
BslFI GGGAC 1 cut(s) 681
BslI CCNNNNNNNGG 2 cut(s) 197, 663
BsmAI GTCTC 1 cut(s) 878
BsmFI GGGAC 1 cut(s) 681
BsnI GGCC 1 cut(s) 845
Bsp1286I GDGCHC 1 cut(s) 978
Bsp1407I TGTACA 1 cut(s) 274
Bsp143I GATC 3 cut(s) 361, 366, 787
BspACI CCGC 4 cut(s) 14, 516, 626, 676
BspANI GGCC 1 cut(s) 845
BspCNI CTCAG 3 cut(s) 409, 715, 964
BspFNI CGCG 1 cut(s) 14
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 3 cut(s) 19, 669, 860
BspPI GGATC 2 cut(s) 369, 795
BspQI GCTCTTC 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 190
BsrGI TGTACA 1 cut(s) 274
BsrI ACTGG 2 cut(s) 744, 938
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 5 cut(s) 71, 72, 671, 699, 862
BssMI GATC 3 cut(s) 361, 366, 787
BssT1I CCWWGG 2 cut(s) 671, 699
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 2 cut(s) 53, 122
Bst6I CTCTTC 4 cut(s) 242, 405, 426, 592
BstAUI TGTACA 1 cut(s) 274
BstC8I GCNNGC 3 cut(s) 192, 342, 687
BstDEI CTNAG 3 cut(s) 417, 723, 972
BstF5I GGATG 2 cut(s) 238, 775
BstFNI CGCG 1 cut(s) 14
BstKTI GATC 3 cut(s) 364, 369, 790
BstMAI GTCTC 1 cut(s) 878
BstMBI GATC 3 cut(s) 361, 366, 787
BstMWI GCNNNNNNNGC 5 cut(s) 11, 506, 515, 682, 728
BstNI CCWGG 1 cut(s) 73
BstNSI RCATGY 2 cut(s) 622, 811
BstSCI CCNGG 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 310
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 55, 494
BstV2I GAAGAC 1 cut(s) 645
Bsu36I CCTNAGG 1 cut(s) 417
BsuRI GGCC 1 cut(s) 845
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 2 cut(s) 238, 775
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 2 cut(s) 49, 582
Cac8I GCNNGC 3 cut(s) 192, 342, 687
CciI TCATGA 1 cut(s) 591
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 2 cut(s) 668, 826
CseI GACGC 1 cut(s) 728
Csp6I GTAC 3 cut(s) 142, 275, 401
CviAII CATG 7 cut(s) 99, 406, 467, 592, 619, 803, 808
CviQI GTAC 3 cut(s) 142, 275, 401
DdeI CTNAG 3 cut(s) 417, 723, 972
DpnI GATC 3 cut(s) 363, 368, 789
DpnII GATC 3 cut(s) 361, 366, 787
Eam1104I CTCTTC 4 cut(s) 242, 405, 426, 592
EarI CTCTTC 4 cut(s) 242, 405, 426, 592
EciI GGCGGA 1 cut(s) 691
Eco130I CCWWGG 2 cut(s) 671, 699
Eco147I AGGCCT 1 cut(s) 845
Eco47I GGWCC 2 cut(s) 668, 826
Eco57I CTGAAG 3 cut(s) 183, 408, 450
Eco81I CCTNAGG 1 cut(s) 417
EcoRII CCWGG 1 cut(s) 71
EcoT14I CCWWGG 2 cut(s) 671, 699
ErhI CCWWGG 2 cut(s) 671, 699
FaeI CATG 7 cut(s) 102, 409, 470, 595, 622, 806, 811
FaqI GGGAC 1 cut(s) 681
FatI CATG 7 cut(s) 98, 405, 466, 591, 618, 802, 807
FauI CCCGC 1 cut(s) 7
Fnu4HI GCNGC 2 cut(s) 44, 483
FokI GGATG 2 cut(s) 245, 782
Fsp4HI GCNGC 2 cut(s) 44, 483
FspBI CTAG 3 cut(s) 138, 645, 985
GluI GCNGC 2 cut(s) 44, 483
GsuI CTGGAG 2 cut(s) 333, 377
HaeIII GGCC 1 cut(s) 845
HapII CCGG 3 cut(s) 134, 191, 501
HgaI GACGC 1 cut(s) 728
Hin1II CATG 7 cut(s) 102, 409, 470, 595, 622, 806, 811
HincII GTYRAC 2 cut(s) 169, 718
HindII GTYRAC 2 cut(s) 169, 718
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 2 cut(s) 776, 968
HpaI GTTAAC 1 cut(s) 169
HpaII CCGG 3 cut(s) 134, 191, 501
HphI GGTGA 2 cut(s) 505, 688
Hpy166II GTNNAC 3 cut(s) 169, 275, 718
Hpy188III TCNNGA 8 cut(s) 64, 221, 350, 560, 592, 645, 791, 881
Hpy8I GTNNAC 3 cut(s) 169, 275, 718
HpyAV CCTTC 4 cut(s) 75, 383, 498, 721
HpyCH4III ACNGT 2 cut(s) 53, 122
HpyCH4IV ACGT 2 cut(s) 333, 640
HpyCH4V TGCA 3 cut(s) 43, 92, 242
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 506, 515, 682, 728
HpyF3I CTNAG 3 cut(s) 417, 723, 972
HpySE526I ACGT 2 cut(s) 333, 640
Hsp92II CATG 7 cut(s) 102, 409, 470, 595, 622, 806, 811
KroI GCCGGC 1 cut(s) 190
KroNI GCCGGC 1 cut(s) 192
KspAI GTTAAC 1 cut(s) 169
Kzo9I GATC 3 cut(s) 361, 366, 787
LguI GCTCTTC 1 cut(s) 405
LmnI GCTCC 1 cut(s) 864
Lsp1109I GCAGC 2 cut(s) 55, 494
LweI GCATC 2 cut(s) 57, 353
MaeI CTAG 3 cut(s) 138, 645, 985
MaeII ACGT 2 cut(s) 333, 640
MaeIII GTNAC 1 cut(s) 314
MalI GATC 3 cut(s) 363, 368, 789
MboI GATC 3 cut(s) 361, 366, 787
MboII GAAGA 6 cut(s) 150, 259, 422, 443, 609, 645
MfeI CAATTG 1 cut(s) 38
MhlI GDGCHC 1 cut(s) 978
MluCI AATT 8 cut(s) 6, 38, 93, 215, 491, 524, 732, 798
MroNI GCCGGC 1 cut(s) 190
MseI TTAA 5 cut(s) 168, 258, 735, 797, 956
MspI CCGG 3 cut(s) 134, 191, 501
MspR9I CCNGG 1 cut(s) 73
MunI CAATTG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 73
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 5 cut(s) 11, 506, 515, 682, 728
NaeI GCCGGC 1 cut(s) 192
NdeII GATC 3 cut(s) 361, 366, 787
NgoMIV GCCGGC 1 cut(s) 190
NlaIII CATG 7 cut(s) 102, 409, 470, 595, 622, 806, 811
NlaIV GGNNCC 3 cut(s) 19, 669, 860
NspI RCATGY 2 cut(s) 622, 811
PagI TCATGA 1 cut(s) 591
PasI CCCWGGG 1 cut(s) 72
PceI AGGCCT 1 cut(s) 845
PciSI GCTCTTC 1 cut(s) 405
PdiI GCCGGC 1 cut(s) 192
PfeI GAWTC 2 cut(s) 776, 968
PkrI GCNGC 2 cut(s) 45, 484
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 3 cut(s) 19, 669, 860
PspPI GGNCC 2 cut(s) 668, 826
RsaI GTAC 3 cut(s) 143, 276, 402
RsaNI GTAC 3 cut(s) 142, 275, 401
SapI GCTCTTC 1 cut(s) 405
SaqAI TTAA 5 cut(s) 168, 258, 735, 797, 956
SatI GCNGC 2 cut(s) 44, 483
Sau3AI GATC 3 cut(s) 361, 366, 787
Sau96I GGNCC 2 cut(s) 668, 826
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 1 cut(s) 978
SfaNI GCATC 2 cut(s) 57, 353
SfcI CTRYAG 1 cut(s) 310
SinI GGWCC 2 cut(s) 668, 826
SmlI CTYRAG 2 cut(s) 658, 879
SmoI CTYRAG 2 cut(s) 658, 879
Sse9I AATT 8 cut(s) 6, 38, 93, 215, 491, 524, 732, 798
SseBI AGGCCT 1 cut(s) 845
SsiI CCGC 4 cut(s) 14, 516, 626, 676
SspMI CTAG 3 cut(s) 138, 645, 985
StuI AGGCCT 1 cut(s) 845
StyD4I CCNGG 1 cut(s) 71
StyI CCWWGG 2 cut(s) 671, 699
TaaI ACNGT 2 cut(s) 53, 122
TaiI ACGT 2 cut(s) 336, 643
TaqI TCGA 3 cut(s) 544, 848, 907
TasI AATT 8 cut(s) 6, 38, 93, 215, 491, 524, 732, 798
TatI WGTACW 2 cut(s) 274, 400
TfiI GAWTC 2 cut(s) 776, 968
Tru1I TTAA 5 cut(s) 168, 258, 735, 797, 956
Tru9I TTAA 5 cut(s) 168, 258, 735, 797, 956
TscAI CASTG 2 cut(s) 56, 582
TseI GCWGC 2 cut(s) 43, 482
TspDTI ATGAA 5 cut(s) 87, 114, 483, 580, 791
TspRI CASTG 2 cut(s) 56, 582
VpaK11BI GGWCC 2 cut(s) 668, 826
XapI RAATTY 1 cut(s) 215
XbaI TCTAGA 1 cut(s) 644
XceI RCATGY 2 cut(s) 622, 811
XspI CTAG 3 cut(s) 138, 645, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.