FvH4_4g00480

Type 1 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005349
Physical Location & Seq
Reverse (-)
449102 .. 454265
5164 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00480.t1

Sequence Viewer

Length: 1134 bp
ATGGATTCGCTCGGACGCTTCGTCTGCTTCTATCTGCTCACAGTTTCCTCCTCCTTCTTCTTCTCGCAAGCTAGGGGACAAGGTAGTGCTTCAGTCTTCTTCATCGACAGCTCTACTCATCGATTCCTTCGTAGCCCGGCCTCAAACGATGTCGTTTTCAAGCCTGACTCAATGTCGCTACCGGAAGTCAGTGCTGCCGTGTCTGTCTTGCTTGGATTCGCACCGCCTGCTTCACTCTCAGCTGCCGGTTCATCTAAGCTAAATGAGGTTCTTGTGCCCAATCCATTCAATAGACCTCGTGCTGTTTTCGTTCTGGAAGTCAGAGGAACTGCTGATCCTACTCTTGCTGTCAATGAGAATTCCATGTTTAAAAGTGCTTATGAAACCAAGATTGACCTTGGCTTGAATAAAGCAGAGATTGAGCTTCCTGATGAAAGTGAAGTATCTGTGTTCTCTTTGGATGAGCAATCGGCAGATTATACCGAAAACGAAATCAGCGATTTTGCCTCTTGGATGGGCGGATTATATGTTGCTGATACATTGGAAGTGCTAAATGGAGAGTTGACCTTCCCCTTGGCCAAAGGCGATCAGTTGAAGCTTCATATGTCCAAGGAAGCAGACAGGAAATTCACAACTAGCCTTTTGGTGCTTGTTCGCAATATTAGAAGGGCAATGGAGTTGCATGAAGATTTGATGCACAGTACTCACAGTCCAGCTGAGCTATTAACAGGCAGTTATGATGGCATCAAGGTTTTGGAAGAACAGTATGGATCTGAAGGTGTTGCTCAACATGGGATGGAACTGCTCGTTGCTGTGATGACAAAGATATTTGATTCACTTGAACATGCATACAAAGGTCAAATTGTTGGAGCTATCCTCTTCAATGAAGTAGCACATGTGGAGTCAGGGAAGATGTTGAATGTGATGTACACCAGTCGACCATCTGCTCGTTGGTTGGCAGAAACAAAGACCTCATTAAATACAACTATTGTGAAAGTGCTGTTGGTTAGAAGAACACTTGCTTGGATCACAGGAATTATCCTTCTGATTTCAACTCTCTTGGGGGTATGCTTCCTTGTGAATATGCCAATCACCAAGGATACGTTACTGTACTCTAATGTCAAGCTGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 map04614
Pfam Domains
Protein Families
No domains found.

Protein Analysis

378

Amino Acids

41.47

Weight (kDa)

5.21

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7794 PF25070 29 - 296 2.5e-119 Domain of unknown function (DUF7794)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 937
AciI CCGC 2 cut(s) 224, 519
AclWI GGATC 3 cut(s) 329, 778, 1034
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 2 cut(s) 358, 626
AcuI CTGAAG 2 cut(s) 75, 795
AfaI GTAC 3 cut(s) 703, 929, 1112
AflIII ACRYGT 1 cut(s) 895
AgsI TTSAA 8 cut(s) 160, 289, 406, 595, 842, 883, 919, 1053
AhdI GACNNNNNGTC 2 cut(s) 20, 172
AjuI GAANNNNNNNTTGG 4 cut(s) 986, 1006, 1018, 1038
AloI GAACNNNNNNTCC 2 cut(s) 319, 351
AlwI GGATC 3 cut(s) 329, 778, 1034
AoxI GGCC 2 cut(s) 138, 576
ApeKI GCWGC 2 cut(s) 194, 242
ApoI RAATTY 2 cut(s) 358, 626
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 1 cut(s) 1084
BaeGI GKGCMC 1 cut(s) 279
BalI TGGCCA 1 cut(s) 578
BauI CACGAG 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 88
BbvI GCAGC 2 cut(s) 181, 229
BccI CCATC 4 cut(s) 508, 734, 790, 949
BceAI ACGGC 1 cut(s) 182
BciVI GTATCC 1 cut(s) 1093
BcnI CCSGG 1 cut(s) 137
BfaI CTAG 3 cut(s) 72, 636, 1132
BfuI GTATCC 1 cut(s) 1093
BisI GCNGC 2 cut(s) 195, 243
BlpI GCTNAGC 1 cut(s) 717
BlsI GCNGC 2 cut(s) 196, 244
BmcAI AGTACT 1 cut(s) 703
Bme1390I CCNGG 1 cut(s) 137
BmeRI GACNNNNNGTC 2 cut(s) 20, 172
BmrFI CCNGG 1 cut(s) 137
BmsI GCATC 2 cut(s) 684, 753
BpiI GAAGAC 1 cut(s) 88
BplI GAGNNNNNCTC 2 cut(s) 861, 893
Bpu1102I GCTNAGC 1 cut(s) 717
BpuMI CCSGG 1 cut(s) 137
Bsa29I ATCGAT 1 cut(s) 121
BsaJI CCNNGG 4 cut(s) 397, 573, 609, 1095
BsaWI WCCGGW 1 cut(s) 181
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 933
Bse3DI GCAATG 1 cut(s) 678
BseCI ATCGAT 1 cut(s) 121
BseDI CCNNGG 4 cut(s) 397, 573, 609, 1095
BseGI GGATG 3 cut(s) 466, 519, 801
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 2 cut(s) 252, 708
BseNI ACTGG 1 cut(s) 933
BseRI GAGGAG 1 cut(s) 40
BseSI GKGCMC 1 cut(s) 279
BseXI GCAGC 2 cut(s) 181, 229
BshFI GGCC 2 cut(s) 140, 578
BshVI ATCGAT 1 cut(s) 121
BsiSI CCGG 3 cut(s) 137, 182, 246
BslFI GGGAC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 90
BsnI GGCC 2 cut(s) 140, 578
Bsp1286I GDGCHC 1 cut(s) 279
Bsp1407I TGTACA 1 cut(s) 927
Bsp143I GATC 4 cut(s) 334, 586, 770, 1026
Bsp1720I GCTNAGC 1 cut(s) 717
BspACI CCGC 2 cut(s) 224, 519
BspANI GGCC 2 cut(s) 140, 578
BspCNI CTCAG 2 cut(s) 251, 709
BspDI ATCGAT 1 cut(s) 121
BspPI GGATC 3 cut(s) 329, 778, 1034
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 245
BsrGI TGTACA 1 cut(s) 927
BsrI ACTGG 1 cut(s) 933
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 4 cut(s) 397, 573, 609, 1095
BssMI GATC 4 cut(s) 334, 586, 770, 1026
BssSI CACGAG 1 cut(s) 297
BssT1I CCWWGG 4 cut(s) 397, 573, 609, 1095
Bst2BI CACGAG 1 cut(s) 297
Bst4CI ACNGT 5 cut(s) 43, 701, 710, 765, 1110
Bst6I CTCTTC 1 cut(s) 884
BstAPI GCANNNNNTGC 1 cut(s) 227
BstAUI TGTACA 1 cut(s) 927
BstC8I GCNNGC 2 cut(s) 69, 228
BstDEI CTNAG 3 cut(s) 238, 255, 717
BstF5I GGATG 3 cut(s) 466, 519, 801
BstKTI GATC 4 cut(s) 337, 589, 773, 1029
BstMBI GATC 4 cut(s) 334, 586, 770, 1026
BstMWI GCNNNNNNNGC 2 cut(s) 24, 227
BstNSI RCATGY 2 cut(s) 848, 899
BstSCI CCNGG 1 cut(s) 135
BstSLI GKGCMC 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 181, 229
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 770
BstYI RGATCY 1 cut(s) 770
Bsu15I ATCGAT 1 cut(s) 121
BsuI GTATCC 1 cut(s) 1093
BsuRI GGCC 2 cut(s) 140, 578
BsuTUI ATCGAT 1 cut(s) 121
BtsCI GGATG 3 cut(s) 466, 519, 801
BtsIMutI CAGTG 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 69, 228
Cfr10I RCCGGY 1 cut(s) 245
ClaI ATCGAT 1 cut(s) 121
CseI GACGC 1 cut(s) 24
Csp6I GTAC 3 cut(s) 702, 928, 1111
CviAII CATG 5 cut(s) 364, 683, 791, 845, 896
CviQI GTAC 3 cut(s) 702, 928, 1111
DdeI CTNAG 3 cut(s) 238, 255, 717
DpnI GATC 4 cut(s) 336, 588, 772, 1028
DpnII GATC 4 cut(s) 334, 586, 770, 1026
DraI TTTAAA 1 cut(s) 370
DriI GACNNNNNGTC 2 cut(s) 20, 172
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 884
Eam1105I GACNNNNNGTC 2 cut(s) 20, 172
EarI CTCTTC 1 cut(s) 884
EciI GGCGGA 1 cut(s) 534
Eco130I CCWWGG 4 cut(s) 397, 573, 609, 1095
Eco57I CTGAAG 2 cut(s) 75, 795
EcoRI GAATTC 1 cut(s) 358
EcoT14I CCWWGG 4 cut(s) 397, 573, 609, 1095
EcoT22I ATGCAT 1 cut(s) 850
ErhI CCWWGG 4 cut(s) 397, 573, 609, 1095
FaeI CATG 5 cut(s) 367, 686, 794, 848, 899
FaqI GGGAC 1 cut(s) 90
FatI CATG 5 cut(s) 363, 682, 790, 844, 895
FauNDI CATATG 1 cut(s) 603
FblI GTMKAC 1 cut(s) 937
Fnu4HI GCNGC 2 cut(s) 195, 243
FokI GGATG 3 cut(s) 473, 526, 808
Fsp4HI GCNGC 2 cut(s) 195, 243
FspBI CTAG 3 cut(s) 72, 636, 1132
GluI GCNGC 2 cut(s) 195, 243
HaeIII GGCC 2 cut(s) 140, 578
HapII CCGG 3 cut(s) 137, 182, 246
HgaI GACGC 1 cut(s) 24
Hin1II CATG 5 cut(s) 367, 686, 794, 848, 899
HincII GTYRAC 2 cut(s) 564, 938
HindII GTYRAC 2 cut(s) 564, 938
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 6 cut(s) 5, 123, 167, 216, 833, 902
HpaII CCGG 3 cut(s) 137, 182, 246
HphI GGTGA 1 cut(s) 1084
Hpy166II GTNNAC 3 cut(s) 564, 930, 938
Hpy188I TCNGA 4 cut(s) 14, 323, 775, 1047
Hpy188III TCNNGA 2 cut(s) 314, 428
Hpy8I GTNNAC 3 cut(s) 564, 930, 938
HpyAV CCTTC 6 cut(s) 64, 137, 577, 660, 770, 1052
HpyCH4III ACNGT 5 cut(s) 43, 701, 710, 765, 1110
HpyCH4IV ACGT 1 cut(s) 1103
HpyCH4V TGCA 3 cut(s) 682, 697, 848
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 227
HpyF3I CTNAG 3 cut(s) 238, 255, 717
HpySE526I ACGT 1 cut(s) 1103
Hsp92II CATG 5 cut(s) 367, 686, 794, 848, 899
Kzo9I GATC 4 cut(s) 334, 586, 770, 1026
LmnI GCTCC 1 cut(s) 869
Lsp1109I GCAGC 2 cut(s) 181, 229
LweI GCATC 2 cut(s) 684, 753
MaeI CTAG 3 cut(s) 72, 636, 1132
MaeII ACGT 1 cut(s) 1103
MaeIII GTNAC 1 cut(s) 1104
MalI GATC 4 cut(s) 336, 588, 772, 1028
MboI GATC 4 cut(s) 334, 586, 770, 1026
MboII GAAGA 9 cut(s) 49, 52, 88, 91, 698, 770, 871, 922, 1023
MflI RGATCY 1 cut(s) 770
MhlI GDGCHC 1 cut(s) 279
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 4 cut(s) 358, 626, 861, 1035
MluNI TGGCCA 1 cut(s) 578
MlyI GAGTC 2 cut(s) 161, 911
MmeI TCCRAC 1 cut(s) 847
MnlI CCTC 9 cut(s) 58, 61, 151, 259, 306, 317, 517, 887, 982
Mox20I TGGCCA 1 cut(s) 578
Mph1103I ATGCAT 1 cut(s) 850
MscI TGGCCA 1 cut(s) 578
MseI TTAA 3 cut(s) 369, 725, 977
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 2 cut(s) 242, 716
MspI CCGG 3 cut(s) 137, 182, 246
MspR9I CCNGG 1 cut(s) 137
MwoI GCNNNNNNNGC 2 cut(s) 24, 227
NciI CCSGG 1 cut(s) 137
NdeI CATATG 1 cut(s) 603
NdeII GATC 4 cut(s) 334, 586, 770, 1026
NlaIII CATG 5 cut(s) 367, 686, 794, 848, 899
NsiI ATGCAT 1 cut(s) 850
NspI RCATGY 2 cut(s) 848, 899
PciI ACATGT 1 cut(s) 895
PcsI WCGNNNNNNNCGW 3 cut(s) 18, 127, 495
PfeI GAWTC 4 cut(s) 5, 123, 216, 833
PkrI GCNGC 2 cut(s) 196, 244
PleI GAGTC 2 cut(s) 161, 910
PpsI GAGTC 2 cut(s) 161, 910
PscI ACATGT 1 cut(s) 895
PsuI RGATCY 1 cut(s) 770
PvuII CAGCTG 2 cut(s) 242, 716
RsaI GTAC 3 cut(s) 703, 929, 1112
RsaNI GTAC 3 cut(s) 702, 928, 1111
SalI GTCGAC 1 cut(s) 936
SaqAI TTAA 3 cut(s) 369, 725, 977
SatI GCNGC 2 cut(s) 195, 243
Sau3AI GATC 4 cut(s) 334, 586, 770, 1026
ScaI AGTACT 1 cut(s) 703
SchI GAGTC 2 cut(s) 161, 911
ScrFI CCNGG 1 cut(s) 137
SduI GDGCHC 1 cut(s) 279
SfaNI GCATC 2 cut(s) 684, 753
Sse9I AATT 4 cut(s) 358, 626, 861, 1035
SsiI CCGC 2 cut(s) 224, 519
SspI AATATT 1 cut(s) 661
SspMI CTAG 3 cut(s) 72, 636, 1132
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 4 cut(s) 397, 573, 609, 1095
TaaI ACNGT 5 cut(s) 43, 701, 710, 765, 1110
TaiI ACGT 1 cut(s) 1106
TaqI TCGA 3 cut(s) 105, 121, 937
TasI AATT 4 cut(s) 358, 626, 861, 1035
TatI WGTACW 3 cut(s) 701, 927, 1110
TfiI GAWTC 4 cut(s) 5, 123, 216, 833
Tru1I TTAA 3 cut(s) 369, 725, 977
Tru9I TTAA 3 cut(s) 369, 725, 977
TscAI CASTG 1 cut(s) 196
TseI GCWGC 2 cut(s) 194, 242
TspDTI ATGAA 7 cut(s) 91, 240, 396, 447, 590, 699, 900
TspRI CASTG 1 cut(s) 196
XapI RAATTY 2 cut(s) 358, 626
XceI RCATGY 2 cut(s) 848, 899
XcmI CCANNNNNNNNNTGG 1 cut(s) 948
XmiI GTMKAC 1 cut(s) 937
XspI CTAG 3 cut(s) 72, 636, 1132
ZrmI AGTACT 1 cut(s) 703
Zsp2I ATGCAT 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.