Rorug03G0302700

Type 1 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005349
Physical Location & Seq
Forward (+)
31973101 .. 31977662
4562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0302700.1

Sequence Viewer

Length: 3741 bp
ATGGAGTTCTATCAGTGTGTCTTTGAGACGATGAGGATGGTCATTGTGCTTAGTGTGGTAATGGTGCAATGGTTCGGTATCATAGCCAGTCTTAAGTGGATATGGAAGAATTGGTTCATTATTGTGGTGACATGGAACGTAATAGCCAGTCTTGGGTGGATATGGAAGAGTAGGTTCAGTTACATGGCTGAAACCCCAGAGCCAGTATCAGAGGATAATCAAGAACTGGTTGACGTCGATGCCTCTGCATCTCTTCCTTCATCGTCAGCTGAATCATCAGCTCCTAGGTGGAAGCATGATGTGTTTTTGAGTTTCAGGGGTGAGGACACTCGTCGGGGTATTACATCCTTTTTATACGATCGACTGAAAAACAGAGGAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTACTGGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTCCAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATTTGTGAATGCATGAAAGAAGACGACAATAGAATTCTGCCACTTTTTTATAATGTGGATCCTAGTGATGTACGAAATCAGAAGAGGAGTTTTGGAGATGCTTTCACTAAGCATGAAAAATCTGGGAAACACAAATCAGAAAAGGTGCAGCAGTGGAGAGATGCTTTAACAAAAGTGGCAAATTTCTCTGGATGGCATACACTGAATTATAAAACTGATAGAGAACTTGTGGATGCCATTGAGGAATCTGTGTGCAATAAATTACGACTGACTGAACCTGTGCTTAAAATGTGCATCGGAGGTTTTAAAGAATTTGCAGCAACAAAACAAGCCATTGATAAGGTCATGAACGCGCTAAAAGATGATGAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTTGGAAAGACGACAATGGTGAAATATGTTGGAGCACAAGCCCAAAAATGTAGGCTTTTTAATCAAGTGATTATGCCTGTCGTATCCCAAAACCCCGACTTGATGAAAATTCAAGAGACATTTGCAGAACTGCTGGGCTTGAAATTGGAGGAGAAGACAGAAATTGGAAGAGCCAGTAAATTGAAGGAGAAGATATTGAAACGAACTGGGATCCTCATAATCTTGGATGACATTTGGAAGAGCATAGACTTTTCAAGCATTGGAATTCCAAGCCACAACGAACTTCAAAGATGCAATTCCAAAGTTCTACTGACTACCAGAAAATTGACAGTTTGCAATTCCATGGATTGCCATGCAAACATACATCTCAATATCTTATCAGAAGAAGATTCTTGGAGCTTATTTGTGAAGGAAGCAAGGAAGTACCCTCTTGACAAATCATCCACTTTCTATGATGTAGCACGTAAGGTGGCTAGAGAATGCGCCGGTCTACCACTTGCTTTGATAGTAGTTGCGAGGGCCCTTCGAGGTCAAGGTTTAGATGGATGGAAAGAAGCTGCTCGACAACTACAAGCGTCTCAACCTCCCAACCCTGAAGATGAGGGAGTTGTGTATGCATCCATAAAGTTAAGCTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAACACTTGCTCATGTATGGAATTGGGAAAGGAATGTTTCAAGATTCCAACATGCTAGAAGCCCGAGACAGAACATATTTAGTGGTGAAGGCCCTTAAAGATTCTAGCTTGCTTTTGGATGCTAGAGATGACGGATTTGTAAGGATGCATGATGTCATTCGGGATGTGGCAATATCAATTTCATTATCTGAAGACGGCCAGCGGTTCTTGGTGAAAAGTGGCTATGAATTGAAGAATTGGCCGAAGATTGATGCACATAAAGGCTACTCTGCAATCTCACTAATGAAGAACAAAATTTGCAAGCTACCGGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTACAACACAATGCTTCTTTAAATGAGATCCCAAAATCATTTTTCCAGAGTCAGAATGAATTAAGAGTCTTGGATCTTAGCTACACTGGTATTTCATTACTACCCCAATCAATCAGTTCTCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCGGGAACATAATTGACATTTCTGTAATCGGAAAACTTCACAAGCTTGAGATTCTTAGCATGAGAAGATATGCTCTGAAAGAATTGTCAAGAGAAATAGGACAGTTGACCAATCTAAGGATGCTGGATGTCAGTGCTGTACATATTCGCACAATCCCATCTAAAGTGATTACCAAGTTGCATAAGTTAGAAGAACTGTACATGGAATGTGAATTTTTGGACTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACTAATATTGGCTTGGATGAATTAGCTGGTTTATTACATTTAAGCATTTTGACAGTTTGCATATCGAATGCAAATGGCATCCCTAAAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTCGATATCAGTATTCGCAGCGGCCATAATGGAGAGAGAGACAATGGAAACTTTCGATCCGCTTGTAATTGTAGATCCTTGAGAACTGACACAACCATAGGAACCCTTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAATACTGAGAGGCTGCGTTATAGATATTGCGAAGGGTTAAGTAACATTCTTGTGGAATATGACCGTGGGAGGTTTCATGGACTGAAAGTTCTCTTTGTAGTTGGTGGGTGTGCGAACTTGATAGAATTGATGAATGCAATAACATGTGTTCCACATATGCCTGTGTTTGAGAACTTGGAGTATTTGTATCTGGAAGAACTGTCTCACTTGAGGCAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCTGCAGTCTGAAATTATTGAAGGTCTCTGGGTGTCCTACTTTTGGGAATGCACTGTTGCCATCAAAATTGTTGCAGAAACTAGCAAATCTGGAAAAACTAATCTGTGAGAGAAGTGGAGTGGAACATGTTTTTGGATGTGAAGGATTTGAGCTAGATCATACAAATCTGAGAGAGATGGTGTTGATGGATCTACCCGCAGTAAGAAGCATGTGTAATGGTCCTGCTCCACGTGGAATGTTCCAGACTCTAAAGAGATTGGTCATTGTGAATTGCAAATTCCGGGGAAGTCTCTTCACATTTGATGTGGCTCAGTGTCTTTTTCAATTGGAAAACCTTGATGTATCCGAATGCCCTGTCTTGGAAAGAGTAATCGAAGCAAGGAGGGAAACATTGAACAACAAGAAGACCGTTCTTCCAAAATTGAAGAACTTATGTTTGCAATCTCTTCCAATGTTGTACGAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAACACTTGCGTATACACGAGTGCCCCCACTTGTCATTTCCATCCTCTGTTTCTGACTACTTCAACAGCAGGAATCATGATCTTTTCAATGGTGGTCATTCTCTGAATCTGATGCGCAGACGGATACTGAGAATTATCGGGTTTACTGATCGATCAAAGCTGGATAGAGTTTGGGTTCTTCTTGATTCATCGAGAAGAAAGCGGACAGTTCAGCGAAAGAGCCCGGTTTCAATCCCAGTGGCTGCACTTGACCTTGATAATGAAGAAACTACAAAAGAAGAGAGTGAGAAAGAGAAGGTAGAGCCTGAAATTGTTGTTGTGAATGAAGAAAAGAAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 map04614
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1246

Amino Acids

141.45

Weight (kDa)

6.41

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 98 - 268 9.5e-53 TIR domain
TIR_2 PF13676 101 - 211 2e-17 TIR domain
NB-ARC PF00931 285 - 450 1.2e-31 NB-ARC domain
LRR_8 PF13855 657 - 715 1e-09 Leucine rich repeat
LRR_14 PF23598 712 - 783 4.5e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 943 - 1084 2.3e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1026 - 1152 1.5e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 579, 738
AasI GACNNNNNNGTC 1 cut(s) 2633
AatII GACGTC 1 cut(s) 237
Acc16I TGCGCA 1 cut(s) 3515
AccI GTMKAC 3 cut(s) 915, 1420, 3412
AccII CGCG 1 cut(s) 881
AccIII TCCGGA 1 cut(s) 2623
AciI CCGC 6 cut(s) 1846, 2145, 2535, 2574, 3096, 3601
AcoI YGGCCR 3 cut(s) 1840, 1883, 2536
AcsI RAATTY 8 cut(s) 561, 709, 839, 1038, 1194, 1938, 2354, 3176
AcuI CTGAAG 2 cut(s) 1545, 1854
AcvI CACGTG 1 cut(s) 3131
AcyI GRCGYC 1 cut(s) 234
AdeI CACNNNGTG 1 cut(s) 507
AfaI GTAC 5 cut(s) 600, 1355, 2283, 2342, 3359
AfiI CCNNNNNNNGG 4 cut(s) 153, 2259, 2942, 3259
AflII CTTAAG 1 cut(s) 92
AflIII ACRYGT 2 cut(s) 2795, 3025
AjnI CCWGG 1 cut(s) 2890
AjuI GAANNNNNNNTTGG 6 cut(s) 1583, 1615, 2388, 2420, 3015, 3047
AloI GAACNNNNNNTCC 2 cut(s) 2724, 2756
Alw21I GWGCWC 1 cut(s) 967
Alw26I GTCTC 8 cut(s) 20, 1040, 1512, 1704, 2547, 2859, 2929, 3194
AlwNI CAGNNNCTG 1 cut(s) 3641
Ama87I CYCGRG 1 cut(s) 1707
Aor13HI TCCGGA 1 cut(s) 2623
AoxI GGCC 6 cut(s) 897, 1449, 1734, 1840, 1883, 2536
ApaI GGGCCC 1 cut(s) 1453
ApeKI GCWGC 7 cut(s) 676, 845, 1487, 1611, 2532, 2665, 3641
ApoI RAATTY 8 cut(s) 561, 709, 839, 1038, 1194, 1938, 2354, 3176
ArsI GACNNNNNNTTYG 2 cut(s) 3240, 3272
Asp700I GAANNNNTTC 1 cut(s) 113
AspA2I CCTAGG 1 cut(s) 284
AspLEI GCGC 3 cut(s) 883, 1415, 3516
AspS9I GGNCC 4 cut(s) 1449, 1450, 1735, 3119
AsuC2I CCSGG 2 cut(s) 3182, 3623
AsuHPI GGTGA 6 cut(s) 139, 332, 961, 1741, 1867, 2891
AvaI CYCGRG 1 cut(s) 1707
AvaII GGWCC 1 cut(s) 3119
AvrII CCTAGG 1 cut(s) 284
BaeGI GKGCMC 2 cut(s) 1453, 3425
BamHI GGATCC 2 cut(s) 586, 1140
BanII GRGCYC 2 cut(s) 1453, 3623
BauI CACGAG 1 cut(s) 3416
BbrPI CACGTG 1 cut(s) 3131
BbsI GAAGAC 4 cut(s) 555, 1091, 1842, 3311
Bbv12I GWGCWC 1 cut(s) 967
BbvI GCAGC 7 cut(s) 688, 857, 1474, 1598, 2544, 2652, 3628
BceAI ACGGC 1 cut(s) 1855
BcgI CGANNNNNNTGC 4 cut(s) 227, 261, 3350, 3384
BciT130I CCWGG 1 cut(s) 2892
BciVI GTATCC 3 cut(s) 1024, 3253, 3516
BcnI CCSGG 2 cut(s) 3182, 3623
BcoDI GTCTC 8 cut(s) 20, 1040, 1512, 1704, 2547, 2859, 2929, 3194
BfaI CTAG 8 cut(s) 285, 591, 1404, 1700, 1749, 1767, 2981, 3053
BfmI CTRYAG 1 cut(s) 2902
BfrI CTTAAG 1 cut(s) 92
BfuI GTATCC 3 cut(s) 1024, 3253, 3516
BisI GCNGC 8 cut(s) 677, 846, 1488, 1612, 2533, 2536, 2666, 3642
BlnI CCTAGG 1 cut(s) 284
BlsI GCNGC 8 cut(s) 678, 847, 1489, 1613, 2534, 2537, 2667, 3643
Bme1390I CCNGG 3 cut(s) 2892, 3182, 3623
Bme18I GGWCC 1 cut(s) 3119
BmeT110I CYCGRG 1 cut(s) 1707
BmgT120I GGNCC 4 cut(s) 1449, 1450, 1735, 3119
BmiI GGNNCC 4 cut(s) 588, 1142, 1451, 2617
BmrFI CCNGG 3 cut(s) 2892, 3182, 3623
BmrI ACTGGG 3 cut(s) 1146, 2374, 3629
BmuI ACTGGG 3 cut(s) 1146, 2374, 3629
BoxI GACNNNNGTC 1 cut(s) 330
BpiI GAAGAC 4 cut(s) 555, 1091, 1842, 3311
BpmI CTGGAG 1 cut(s) 1961
BpuEI CTTGAG 3 cut(s) 2210, 2614, 2881
BpuMI CCSGG 2 cut(s) 3182, 3623
Bsa29I ATCGAT 1 cut(s) 3550
BsaAI YACGTR 2 cut(s) 1394, 3131
BsaHI GRCGYC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 2929
BsaJI CCNNGG 4 cut(s) 284, 1272, 2716, 3181
BsaWI WCCGGW 2 cut(s) 1951, 2623
BsaXI ACNNNNNCTCC 2 cut(s) 2822, 2852
Bsc4I CCNNNNNNNGG 4 cut(s) 153, 2259, 2942, 3259
Bse118I RCCGGY 1 cut(s) 1415
Bse3DI GCAATG 1 cut(s) 74
BseAI TCCGGA 1 cut(s) 2623
BseBI CCWGG 1 cut(s) 2892
BseCI ATCGAT 1 cut(s) 3550
BseDI CCNNGG 4 cut(s) 284, 1272, 2716, 3181
BseLI CCNNNNNNNGG 4 cut(s) 153, 2259, 2942, 3259
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 4 cut(s) 2649, 3059, 3224, 3518
BseRI GAGGAG 2 cut(s) 628, 1094
BseSI GKGCMC 2 cut(s) 1453, 3425
BseXI GCAGC 7 cut(s) 688, 857, 1474, 1598, 2544, 2652, 3628
BseYI CCCAGC 1 cut(s) 1063
BsgI GTGCAG 2 cut(s) 695, 3627
Bsh1236I CGCG 1 cut(s) 881
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 6 cut(s) 899, 1451, 1736, 1842, 1885, 2538
BshVI ATCGAT 1 cut(s) 3550
BsiEI CGRYCG 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 967
BsiHKCI CYCGRG 1 cut(s) 1707
BsiSI CCGG 5 cut(s) 1416, 1952, 2624, 3181, 3623
BslI CCNNNNNNNGG 4 cut(s) 153, 2259, 2942, 3259
BsmAI GTCTC 8 cut(s) 20, 1040, 1512, 1704, 2547, 2859, 2929, 3194
BsmBI CGTCTC 2 cut(s) 20, 1512
BsmI GAATGC 6 cut(s) 542, 1415, 2470, 2791, 2953, 3254
BsnI GGCC 6 cut(s) 899, 1451, 1736, 1842, 1885, 2538
Bso31I GGTCTC 1 cut(s) 2929
BsoBI CYCGRG 1 cut(s) 1707
Bsp120I GGGCCC 1 cut(s) 1449
Bsp1286I GDGCHC 4 cut(s) 967, 1453, 3425, 3623
Bsp13I TCCGGA 1 cut(s) 2623
Bsp1407I TGTACA 2 cut(s) 2281, 2340
Bsp19I CCATGG 1 cut(s) 1272
BspACI CCGC 6 cut(s) 1846, 2145, 2535, 2574, 3096, 3601
BspANI GGCC 6 cut(s) 899, 1451, 1736, 1842, 1885, 2538
BspCNI CTCAG 4 cut(s) 2650, 3060, 3223, 3519
BspDI ATCGAT 1 cut(s) 3550
BspEI TCCGGA 1 cut(s) 2623
BspFNI CGCG 1 cut(s) 881
BspHI TCATGA 2 cut(s) 873, 3475
BspLI GGNNCC 4 cut(s) 588, 1142, 1451, 2617
BspMAI CTGCAG 1 cut(s) 2906
BspQI GCTCTTC 2 cut(s) 1093, 1163
BspTI CTTAAG 1 cut(s) 92
BspTNI GGTCTC 1 cut(s) 2929
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 1415
BsrGI TGTACA 2 cut(s) 2281, 2340
BssAI RCCGGY 1 cut(s) 1415
BssECI CCNNGG 4 cut(s) 284, 1272, 2716, 3181
BssNAI GTATAC 1 cut(s) 3413
BssNI GRCGYC 1 cut(s) 234
BssSI CACGAG 1 cut(s) 3416
BssT1I CCWWGG 2 cut(s) 284, 1272
Bst1107I GTATAC 1 cut(s) 3413
Bst2BI CACGAG 1 cut(s) 3416
Bst2UI CCWGG 1 cut(s) 2892
Bst4CI ACNGT 9 cut(s) 1261, 2247, 2340, 2452, 2717, 2853, 2955, 3310, 3607
BstACI GRCGYC 1 cut(s) 234
BstAFI CTTAAG 1 cut(s) 92
BstAUI TGTACA 2 cut(s) 2281, 2340
BstBAI YACGTR 2 cut(s) 1394, 3131
BstC8I GCNNGC 3 cut(s) 1754, 1844, 1946
BstDSI CCRYGG 2 cut(s) 1272, 2716
BstFNI CGCG 1 cut(s) 881
BstHHI GCGC 3 cut(s) 883, 1415, 3516
BstMAI GTCTC 8 cut(s) 20, 1040, 1512, 1704, 2547, 2859, 2929, 3194
BstMCI CGRYCG 1 cut(s) 361
BstNI CCWGG 1 cut(s) 2892
BstNSI RCATGY 4 cut(s) 1699, 2799, 3029, 3112
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 3 cut(s) 2890, 3180, 3621
BstSFI CTRYAG 1 cut(s) 2902
BstSLI GKGCMC 2 cut(s) 1453, 3425
BstUI CGCG 1 cut(s) 881
BstV1I GCAGC 7 cut(s) 688, 857, 1474, 1598, 2544, 2652, 3628
BstV2I GAAGAC 4 cut(s) 555, 1091, 1842, 3311
BstX2I RGATCY 7 cut(s) 586, 1140, 2016, 2062, 2588, 2894, 3088
BstYI RGATCY 7 cut(s) 586, 1140, 2016, 2062, 2588, 2894, 3088
BstZ17I GTATAC 1 cut(s) 3413
Bsu15I ATCGAT 1 cut(s) 3550
BsuI GTATCC 3 cut(s) 1024, 3253, 3516
BsuRI GGCC 6 cut(s) 899, 1451, 1736, 1842, 1885, 2538
BsuTUI ATCGAT 1 cut(s) 3550
BtgI CCRYGG 2 cut(s) 1272, 2716
BtsI GCAGTG 1 cut(s) 686
BtsIMutI CAGTG 8 cut(s) 20, 686, 728, 2073, 2281, 2951, 3218, 3642
Cac8I GCNNGC 3 cut(s) 1754, 1844, 1946
CaiI CAGNNNCTG 1 cut(s) 3641
CciI TCATGA 2 cut(s) 873, 3475
CfoI GCGC 3 cut(s) 883, 1415, 3516
Cfr10I RCCGGY 1 cut(s) 1415
Cfr13I GGNCC 4 cut(s) 1449, 1450, 1735, 3119
ClaI ATCGAT 1 cut(s) 3550
CseI GACGC 1 cut(s) 1494
Csp6I GTAC 5 cut(s) 599, 1354, 2282, 2341, 3358
CspCI CAANNNNNGTGG 6 cut(s) 889, 924, 1413, 1448, 3618, 3653
CviQI GTAC 5 cut(s) 599, 1354, 2282, 2341, 3358
DraI TTTAAA 2 cut(s) 835, 2010
DraIII CACNNNGTG 1 cut(s) 507
DrdI GACNNNNNNGTC 1 cut(s) 2633
DseDI GACNNNNNNGTC 1 cut(s) 2633
EaeI YGGCCR 3 cut(s) 1840, 1883, 2536
Eco130I CCWWGG 2 cut(s) 284, 1272
Eco24I GRGCYC 2 cut(s) 1453, 3623
Eco31I GGTCTC 1 cut(s) 2929
Eco32I GATATC 2 cut(s) 1636, 2521
Eco47I GGWCC 1 cut(s) 3119
Eco57I CTGAAG 2 cut(s) 1545, 1854
Eco72I CACGTG 1 cut(s) 3131
Eco88I CYCGRG 1 cut(s) 1707
EcoO109I RGGNCCY 3 cut(s) 1449, 1450, 1735
EcoRI GAATTC 2 cut(s) 561, 1194
EcoRII CCWGG 1 cut(s) 2890
EcoRV GATATC 2 cut(s) 1636, 2521
EcoT14I CCWWGG 2 cut(s) 284, 1272
EcoT22I ATGCAT 3 cut(s) 542, 1549, 1794
EcoT38I GRGCYC 2 cut(s) 1453, 3623
ErhI CCWWGG 2 cut(s) 284, 1272
Esp3I CGTCTC 2 cut(s) 20, 1512
FauI CCCGC 2 cut(s) 2138, 3103
FauNDI CATATG 1 cut(s) 2808
FblI GTMKAC 3 cut(s) 915, 1420, 3412
Fnu4HI GCNGC 8 cut(s) 677, 846, 1488, 1612, 2533, 2536, 2666, 3642
FriOI GRGCYC 2 cut(s) 1453, 3623
Fsp4HI GCNGC 8 cut(s) 677, 846, 1488, 1612, 2533, 2536, 2666, 3642
FspBI CTAG 8 cut(s) 285, 591, 1404, 1700, 1749, 1767, 2981, 3053
FspI TGCGCA 1 cut(s) 3515
GlaI GCGC 3 cut(s) 882, 1414, 3515
GluI GCNGC 8 cut(s) 677, 846, 1488, 1612, 2533, 2536, 2666, 3642
GsaI CCCAGC 1 cut(s) 1067
GsuI CTGGAG 1 cut(s) 1961
HaeIII GGCC 6 cut(s) 899, 1451, 1736, 1842, 1885, 2538
HapII CCGG 5 cut(s) 1416, 1952, 2624, 3181, 3623
HgaI GACGC 1 cut(s) 1494
HhaI GCGC 3 cut(s) 883, 1415, 3516
Hin1I GRCGYC 1 cut(s) 234
Hin6I GCGC 3 cut(s) 881, 1413, 3514
HinP1I GCGC 3 cut(s) 881, 1413, 3514
HincII GTYRAC 3 cut(s) 232, 2250, 2638
HindII GTYRAC 3 cut(s) 232, 2250, 2638
HindIII AAGCTT 2 cut(s) 2124, 2186
HpaII CCGG 5 cut(s) 1416, 1952, 2624, 3181, 3623
HphI GGTGA 6 cut(s) 139, 332, 961, 1741, 1867, 2891
Hpy166II GTNNAC 8 cut(s) 232, 916, 1421, 2250, 2512, 2638, 3413, 3543
Hpy8I GTNNAC 8 cut(s) 232, 916, 1421, 2250, 2512, 2638, 3413, 3543
Hpy99I CGWCG 2 cut(s) 239, 336
HpyCH4III ACNGT 9 cut(s) 1261, 2247, 2340, 2452, 2717, 2853, 2955, 3310, 3607
HpyCH4IV ACGT 4 cut(s) 138, 234, 1393, 3130
HpySE526I ACGT 4 cut(s) 138, 234, 1393, 3130
Hsp92I GRCGYC 1 cut(s) 234
HspAI GCGC 3 cut(s) 881, 1413, 3514
Kpn2I TCCGGA 1 cut(s) 2623
LguI GCTCTTC 2 cut(s) 1093, 1163
LmnI GCTCC 4 cut(s) 286, 962, 1326, 3130
Lsp1109I GCAGC 7 cut(s) 688, 857, 1474, 1598, 2544, 2652, 3628
MaeI CTAG 8 cut(s) 285, 591, 1404, 1700, 1749, 1767, 2981, 3053
MaeII ACGT 4 cut(s) 138, 234, 1393, 3130
MaeIII GTNAC 4 cut(s) 127, 179, 2693, 2883
MfeI CAATTG 2 cut(s) 1641, 3224
MflI RGATCY 7 cut(s) 586, 1140, 2016, 2062, 2588, 2894, 3088
MhlI GDGCHC 4 cut(s) 967, 1453, 3425, 3623
MlyI GAGTC 4 cut(s) 921, 2047, 2064, 3139
MmeI TCCRAC 3 cut(s) 913, 940, 1716
Mph1103I ATGCAT 3 cut(s) 542, 1549, 1794
MroI TCCGGA 1 cut(s) 2623
MroXI GAANNNNTTC 1 cut(s) 113
MslI CAYNNNNRTG 2 cut(s) 122, 2702
MspA1I CMGCKG 3 cut(s) 269, 1846, 2535
MspCI CTTAAG 1 cut(s) 92
MspI CCGG 5 cut(s) 1416, 1952, 2624, 3181, 3623
MspR9I CCNGG 3 cut(s) 2892, 3182, 3623
MunI CAATTG 2 cut(s) 1641, 3224
Mva1269I GAATGC 6 cut(s) 542, 1415, 2470, 2791, 2953, 3254
MvaI CCWGG 1 cut(s) 2892
MvnI CGCG 1 cut(s) 881
NciI CCSGG 2 cut(s) 3182, 3623
NcoI CCATGG 1 cut(s) 1272
NdeI CATATG 1 cut(s) 2808
NlaIV GGNNCC 4 cut(s) 588, 1142, 1451, 2617
NmuCI GTSAC 1 cut(s) 127
NsbI TGCGCA 1 cut(s) 3515
NsiI ATGCAT 3 cut(s) 542, 1549, 1794
NspI RCATGY 4 cut(s) 1699, 2799, 3029, 3112
PagI TCATGA 2 cut(s) 873, 3475
PciI ACATGT 2 cut(s) 2795, 3025
PciSI GCTCTTC 2 cut(s) 1093, 1163
PctI GAATGC 6 cut(s) 542, 1415, 2470, 2791, 2953, 3254
PdmI GAANNNNTTC 1 cut(s) 113
PkrI GCNGC 8 cut(s) 678, 847, 1489, 1613, 2534, 2537, 2667, 3643
Ple19I CGATCG 1 cut(s) 361
PleI GAGTC 4 cut(s) 920, 2046, 2063, 3139
PmaCI CACGTG 1 cut(s) 3131
PmlI CACGTG 1 cut(s) 3131
PpsI GAGTC 4 cut(s) 920, 2046, 2063, 3139
Ppu21I YACGTR 2 cut(s) 1394, 3131
PscI ACATGT 2 cut(s) 2795, 3025
PshAI GACNNNNGTC 1 cut(s) 330
PsiI TTATAA 2 cut(s) 579, 738
Psp6I CCWGG 1 cut(s) 2890
PspCI CACGTG 1 cut(s) 3131
PspFI CCCAGC 1 cut(s) 1063
PspGI CCWGG 1 cut(s) 2890
PspN4I GGNNCC 4 cut(s) 588, 1142, 1451, 2617
PspOMI GGGCCC 1 cut(s) 1449
PspPI GGNCC 4 cut(s) 1449, 1450, 1735, 3119
PstI CTGCAG 1 cut(s) 2906
PstNI CAGNNNCTG 1 cut(s) 3641
PsuI RGATCY 7 cut(s) 586, 1140, 2016, 2062, 2588, 2894, 3088
PvuI CGATCG 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 5 cut(s) 600, 1355, 2283, 2342, 3359
RsaNI GTAC 5 cut(s) 599, 1354, 2282, 2341, 3358
RseI CAYNNNNRTG 2 cut(s) 122, 2702
SapI GCTCTTC 2 cut(s) 1093, 1163
SatI GCNGC 8 cut(s) 677, 846, 1488, 1612, 2533, 2536, 2666, 3642
Sau96I GGNCC 4 cut(s) 1449, 1450, 1735, 3119
SchI GAGTC 4 cut(s) 921, 2047, 2064, 3139
ScrFI CCNGG 3 cut(s) 2892, 3182, 3623
SduI GDGCHC 4 cut(s) 967, 1453, 3425, 3623
SfcI CTRYAG 1 cut(s) 2902
SinI GGWCC 1 cut(s) 3119
SmiMI CAYNNNNRTG 2 cut(s) 122, 2702
SmlI CTYRAG 4 cut(s) 92, 2189, 2593, 2860
SmoI CTYRAG 4 cut(s) 92, 2189, 2593, 2860
SsiI CCGC 6 cut(s) 1846, 2145, 2535, 2574, 3096, 3601
SspI AATATT 1 cut(s) 2404
SspMI CTAG 8 cut(s) 285, 591, 1404, 1700, 1749, 1767, 2981, 3053
StyD4I CCNGG 3 cut(s) 2890, 3180, 3621
StyI CCWWGG 2 cut(s) 284, 1272
TaaI ACNGT 9 cut(s) 1261, 2247, 2340, 2452, 2717, 2853, 2955, 3310, 3607
TaiI ACGT 4 cut(s) 141, 237, 1396, 3133
TatI WGTACW 2 cut(s) 2281, 2340
TauI GCSGC 1 cut(s) 2538
TscAI CASTG 8 cut(s) 20, 686, 735, 2080, 2281, 2958, 3218, 3642
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 7 cut(s) 676, 845, 1487, 1611, 2532, 2665, 3641
Tsp45I GTSAC 1 cut(s) 127
TspGWI ACGGA 3 cut(s) 933, 1791, 3535
TspRI CASTG 8 cut(s) 20, 686, 735, 2080, 2281, 2958, 3218, 3642
Vha464I CTTAAG 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 3119
XapI RAATTY 8 cut(s) 561, 709, 839, 1038, 1194, 1938, 2354, 3176
XceI RCATGY 4 cut(s) 1699, 2799, 3029, 3112
XcmI CCANNNNNNNNNTGG 2 cut(s) 439, 508
XmaJI CCTAGG 1 cut(s) 284
XmiI GTMKAC 3 cut(s) 915, 1420, 3412
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 8 cut(s) 285, 591, 1404, 1700, 1749, 1767, 2981, 3053
ZraI GACGTC 1 cut(s) 235
Zsp2I ATGCAT 3 cut(s) 542, 1549, 1794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.