MD04G1005200.v1.1

Type 1 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005349
Physical Location & Seq
Reverse (-)
629609 .. 634780
5172 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1005200.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGCTTTACTCACACGCACCATCTGCTTCTACATACTCACCATTGCTTCGTTTATCTCCTCCAACGCTAAGGCTGATGGTACTGCCTCTGTCTTCTTCATCGATAGCTCCACTCATCATTTTCTTCGTAACCCTTCGTCAAACGATGTCGTCAAGCCCGACTCAATGTTGCTCTCAGAAGTCAGTGCAGCCGTGTCAGTTTTGCTTGGATTTGCACCATCTTCCGCCCTCTCAGCTGTTGGTTCAGCTAAGTTGAATGAGGTTCTCGTGCCTAATCCATTCAATAGGCCTCGTGCTGTTGTTGCGCTTGAAGTTGGAGGAATTGATGATCATGCTCTTGTAGTCAAAGACAATGCCATGTTCAGCAGTGCCTACAGTTCCAAGATTGACCTTGGCTCAAGCAAAGCTGACATCGAGCTTCCAGATGAAGGTGAAGTTTCTGTGTTTTCTTTGGATGAACAATCGACAGATTATACTGACAAGGAGATTGGAGATTTTGCAGCTTGGATGAGTGGTTCATATGTTGTTGATACATTAGAACCACTAAATGGAGAGTTGAGCATCCCTTTGGCCAATGGCGACAACTTGAAGCTTCATATGTCAAAGGAATCAGATAGGATATTCACAACTAGCCTTCTGTCTCTCATCCGCAACTTTAAAAGGGCAACAGAAATGCATCAAGATTTGTCACACAACATCCATAGCCCAGCTGAGTTATTAACAGGCCGTTTTGATGGAATCAAGGTTTTGCAAGAGCAGTATGGAACTGATGGAGCAGCTCAACATGGAGTGGAGCTACTACTTGCTACACTGTCCAAGATATTTGATTCACTTGAAAATGCCTACAAAGGTCAAATTGTTGGAGCTATCCTTTTCAATGGAGTTGCACCTGTGGAGTCGGGAAAGATGTTGAATGTGATGTATACTAGTCGATCATATGCTCGTTTGTTGGCTGAAAAAGAAGGCCTGAATAATACAAAAATTGTGCAAGTGTTATTGGTTAGGCGTACACTTGCTTGGATCACAGGAATTATTCTTATCATTTCAACTCTTTTGGGGGTGTGCTTCCTTCTGAATATGCCAGTCACCAGGGATACATTACTCTACTCTAATGTCAAGCTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 map04614
Pfam Domains
Protein Families
No domains found.

Protein Analysis

376

Amino Acids

40.68

Weight (kDa)

5.15

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7794 PF25070 28 - 293 2.2e-119 Domain of unknown function (DUF7794)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 548
AccI GTMKAC 1 cut(s) 923
AciI CCGC 2 cut(s) 225, 649
AclWI GGATC 1 cut(s) 1028
AcoI YGGCCR 1 cut(s) 570
AfaI GTAC 2 cut(s) 82, 1009
AfiI CCNNNNNNNGG 2 cut(s) 428, 548
AgsI TTSAA 8 cut(s) 256, 283, 311, 589, 836, 877, 913, 1047
AhlI ACTAGT 1 cut(s) 926
AjnI CCWGG 1 cut(s) 1088
AloI GAACNNNNNNTCC 2 cut(s) 499, 531
Alw26I GTCTC 1 cut(s) 645
AlwI GGATC 1 cut(s) 1028
AoxI GGCC 4 cut(s) 287, 570, 724, 964
ApeKI GCWGC 3 cut(s) 188, 500, 776
AspLEI GCGC 1 cut(s) 307
AsuHPI GGTGA 3 cut(s) 31, 443, 1078
BalI TGGCCA 1 cut(s) 572
BauI CACGAG 2 cut(s) 266, 291
BbsI GAAGAC 1 cut(s) 85
BbvI GCAGC 3 cut(s) 200, 512, 788
BccI CCATC 5 cut(s) 29, 71, 226, 728, 764
BceAI ACGGC 2 cut(s) 176, 711
BciT130I CCWGG 1 cut(s) 1090
BciVI GTATCC 1 cut(s) 1087
BclI TGATCA 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 645
BcuI ACTAGT 1 cut(s) 926
BfaI CTAG 2 cut(s) 630, 927
BfmI CTRYAG 1 cut(s) 373
BfuI GTATCC 1 cut(s) 1087
BisI GCNGC 3 cut(s) 189, 501, 777
BlsI GCNGC 3 cut(s) 190, 502, 778
Bme1390I CCNGG 1 cut(s) 1090
BmrFI CCNGG 1 cut(s) 1090
BmsI GCATC 2 cut(s) 570, 685
BpiI GAAGAC 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 382
Bsa29I ATCGAT 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 391, 1089
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 548
Bse1I ACTGG 1 cut(s) 1082
Bse3DI GCAATG 1 cut(s) 42
BseBI CCWGG 1 cut(s) 1090
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 391, 1089
BseGI GGATG 5 cut(s) 460, 513, 561, 645, 696
BseLI CCNNNNNNNGG 2 cut(s) 428, 548
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 3 cut(s) 189, 246, 702
BseNI ACTGG 1 cut(s) 1082
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 3 cut(s) 200, 512, 788
BseYI CCCAGC 1 cut(s) 706
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 4 cut(s) 289, 572, 726, 966
BshVI ATCGAT 1 cut(s) 102
BslI CCNNNNNNNGG 2 cut(s) 428, 548
BsmAI GTCTC 1 cut(s) 645
BsnI GGCC 4 cut(s) 289, 572, 726, 966
Bsp143I GATC 3 cut(s) 328, 932, 1020
BspACI CCGC 2 cut(s) 225, 649
BspANI GGCC 4 cut(s) 289, 572, 726, 966
BspCNI CTCAG 3 cut(s) 188, 245, 703
BspDI ATCGAT 1 cut(s) 102
BspPI GGATC 1 cut(s) 1028
BsrDI GCAATG 1 cut(s) 42
BsrI ACTGG 1 cut(s) 1082
BssECI CCNNGG 2 cut(s) 391, 1089
BssMI GATC 3 cut(s) 328, 932, 1020
BssNAI GTATAC 1 cut(s) 924
BssSI CACGAG 2 cut(s) 266, 291
BssT1I CCWWGG 1 cut(s) 391
Bst1107I GTATAC 1 cut(s) 924
Bst2BI CACGAG 2 cut(s) 266, 291
Bst2UI CCWGG 1 cut(s) 1090
Bst4CI ACNGT 2 cut(s) 377, 813
BstAPI GCANNNNNTGC 1 cut(s) 24
BstDEI CTNAG 5 cut(s) 69, 175, 232, 249, 711
BstF5I GGATG 5 cut(s) 460, 513, 561, 645, 696
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 3 cut(s) 331, 935, 1023
BstMAI GTCTC 1 cut(s) 645
BstMBI GATC 3 cut(s) 328, 932, 1020
BstMWI GCNNNNNNNGC 3 cut(s) 24, 233, 302
BstNI CCWGG 1 cut(s) 1090
BstSCI CCNGG 1 cut(s) 1088
BstSFI CTRYAG 1 cut(s) 373
BstV1I GCAGC 3 cut(s) 200, 512, 788
BstV2I GAAGAC 1 cut(s) 85
BstZ17I GTATAC 1 cut(s) 924
Bsu15I ATCGAT 1 cut(s) 102
BsuI GTATCC 1 cut(s) 1087
BsuRI GGCC 4 cut(s) 289, 572, 726, 966
BsuTUI ATCGAT 1 cut(s) 102
BtsCI GGATG 5 cut(s) 460, 513, 561, 645, 696
BtsI GCAGTG 1 cut(s) 373
BtsIMutI CAGTG 3 cut(s) 190, 373, 809
CfoI GCGC 1 cut(s) 307
ClaI ATCGAT 1 cut(s) 102
Csp6I GTAC 2 cut(s) 81, 1008
CviAII CATG 3 cut(s) 332, 358, 785
CviQI GTAC 2 cut(s) 81, 1008
DdeI CTNAG 5 cut(s) 69, 175, 232, 249, 711
DpnI GATC 3 cut(s) 330, 934, 1022
DpnII GATC 3 cut(s) 328, 932, 1020
DraI TTTAAA 1 cut(s) 658
EaeI YGGCCR 1 cut(s) 570
EciI GGCGGA 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 391
Eco147I AGGCCT 2 cut(s) 289, 966
EcoRII CCWGG 1 cut(s) 1088
EcoT14I CCWWGG 1 cut(s) 391
EcoT22I ATGCAT 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 3 cut(s) 335, 361, 788
FatI CATG 3 cut(s) 331, 357, 784
FauNDI CATATG 3 cut(s) 520, 597, 937
FbaI TGATCA 1 cut(s) 328
FblI GTMKAC 1 cut(s) 923
Fnu4HI GCNGC 3 cut(s) 189, 501, 777
FokI GGATG 5 cut(s) 467, 520, 548, 632, 683
Fsp4HI GCNGC 3 cut(s) 189, 501, 777
FspBI CTAG 2 cut(s) 630, 927
GlaI GCGC 1 cut(s) 306
GluI GCNGC 3 cut(s) 189, 501, 777
GsaI CCCAGC 1 cut(s) 710
HaeIII GGCC 4 cut(s) 289, 572, 726, 966
HhaI GCGC 1 cut(s) 307
Hin1II CATG 3 cut(s) 335, 361, 788
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 5 cut(s) 161, 608, 738, 827, 896
HphI GGTGA 3 cut(s) 31, 443, 1078
Hpy166II GTNNAC 2 cut(s) 924, 1010
Hpy188I TCNGA 3 cut(s) 178, 613, 1074
Hpy188III TCNNGA 3 cut(s) 422, 680, 900
Hpy8I GTNNAC 2 cut(s) 924, 1010
HpyAV CCTTC 5 cut(s) 144, 422, 644, 956, 1079
HpyCH4III ACNGT 2 cut(s) 377, 813
HpyCH4V TGCA 7 cut(s) 188, 215, 500, 676, 751, 887, 988
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 233, 302
HpyF3I CTNAG 5 cut(s) 69, 175, 232, 249, 711
Hsp92II CATG 3 cut(s) 335, 361, 788
HspAI GCGC 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 328
Kzo9I GATC 3 cut(s) 328, 932, 1020
LmnI GCTCC 4 cut(s) 113, 773, 793, 863
Lsp1109I GCAGC 3 cut(s) 200, 512, 788
LweI GCATC 2 cut(s) 570, 685
MaeI CTAG 2 cut(s) 630, 927
MaeIII GTNAC 3 cut(s) 128, 687, 1084
MalI GATC 3 cut(s) 330, 934, 1022
MboI GATC 3 cut(s) 328, 932, 1020
MboII GAAGA 4 cut(s) 85, 88, 116, 213
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 4 cut(s) 321, 855, 981, 1029
MluNI TGGCCA 1 cut(s) 572
MlyI GAGTC 2 cut(s) 155, 905
MmeI TCCRAC 3 cut(s) 87, 295, 841
MnlI CCTC 6 cut(s) 70, 97, 239, 253, 300, 311
Mox20I TGGCCA 1 cut(s) 572
Mph1103I ATGCAT 1 cut(s) 678
MscI TGGCCA 1 cut(s) 572
MseI TTAA 2 cut(s) 657, 719
Msp20I TGGCCA 1 cut(s) 572
MspA1I CMGCKG 2 cut(s) 236, 710
MspR9I CCNGG 1 cut(s) 1090
MvaI CCWGG 1 cut(s) 1090
MwoI GCNNNNNNNGC 3 cut(s) 24, 233, 302
NdeI CATATG 3 cut(s) 520, 597, 937
NdeII GATC 3 cut(s) 328, 932, 1020
NlaIII CATG 3 cut(s) 335, 361, 788
NmuCI GTSAC 2 cut(s) 687, 1084
NsiI ATGCAT 1 cut(s) 678
PceI AGGCCT 2 cut(s) 289, 966
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PfeI GAWTC 3 cut(s) 608, 738, 827
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 3 cut(s) 190, 502, 778
PleI GAGTC 2 cut(s) 155, 904
PpsI GAGTC 2 cut(s) 155, 904
Psp6I CCWGG 1 cut(s) 1088
PspFI CCCAGC 1 cut(s) 706
PspGI CCWGG 1 cut(s) 1088
PvuII CAGCTG 2 cut(s) 236, 710
RsaI GTAC 2 cut(s) 82, 1009
RsaNI GTAC 2 cut(s) 81, 1008
SaqAI TTAA 2 cut(s) 657, 719
SatI GCNGC 3 cut(s) 189, 501, 777
Sau3AI GATC 3 cut(s) 328, 932, 1020
SchI GAGTC 2 cut(s) 155, 905
ScrFI CCNGG 1 cut(s) 1090
SfaNI GCATC 2 cut(s) 570, 685
SfcI CTRYAG 1 cut(s) 373
SmlI CTYRAG 1 cut(s) 397
SmoI CTYRAG 1 cut(s) 397
SpeI ACTAGT 1 cut(s) 926
Sse9I AATT 4 cut(s) 321, 855, 981, 1029
SseBI AGGCCT 2 cut(s) 289, 966
SsiI CCGC 2 cut(s) 225, 649
SspMI CTAG 2 cut(s) 630, 927
StuI AGGCCT 2 cut(s) 289, 966
StyD4I CCNGG 1 cut(s) 1088
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 2 cut(s) 377, 813
TaqI TCGA 4 cut(s) 102, 414, 464, 931
TasI AATT 4 cut(s) 321, 855, 981, 1029
TfiI GAWTC 3 cut(s) 608, 738, 827
Tru1I TTAA 2 cut(s) 657, 719
Tru9I TTAA 2 cut(s) 657, 719
TscAI CASTG 3 cut(s) 190, 373, 816
TseFI GTSAC 2 cut(s) 687, 1084
TseI GCWGC 3 cut(s) 188, 500, 776
Tsp45I GTSAC 2 cut(s) 687, 1084
TspDTI ATGAA 5 cut(s) 88, 441, 471, 507, 584
TspRI CASTG 3 cut(s) 190, 373, 816
Van91I CCANNNNNTGG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 923
XspI CTAG 2 cut(s) 630, 927
Zsp2I ATGCAT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.