Rorug03G0302800

Type 1 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005349
Physical Location & Seq
Forward (+)
31980180 .. 31983350
3171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0302800.1

Sequence Viewer

Length: 570 bp
ATGGAGGAGGAAACTGAAGCATATGAGAAGCATAGATATCAACTCCAACTCGAGGAGGCCATTAGTAACAATAGCAACAAAACTTGCACGTTCTTAGCTTACAAGCTTTATCTTCGATCTGAGGGATTTGATTGTGTGTGGAGCTCTGTTTGCAGACCAGTGATTGAGCAGAGGAGAAGAGGACGGCTTTGGGCTTGGGTTTGTCAGTCATGGTATACAGAATTTTGTGGAGCAGTTCCTACTAGACTTGCACAGGACTTGGCATTTTCAGTTGCAAGGTTTATACAAAATGGTGGTTCTTTTGCCAACTACTACATGTACCATGGAGGAACGAATTTTGGCCGAACAGCTGGCGGTCCCTTCATTGCAACTAGCTATGACTATGATGCACCTCTAGATGAATATGGTGAGCCAGCTTTAGTGGCTACTGATGAGCTAATAAAATTTATCTATGATAGGTTCTATGCAAAGGAGTATTTCGATTGTTCTTCTGCCATTGTTCCTGTATCTGTTATTTGGTATCGGTTTGGTGTTCTATTCTTATACAGTTCAAACCCTGTTCTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 map04614
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

21.89

Weight (kDa)

5.24

Isoelectric Point (pI)

57.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 56 - 138 1.2e-22 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 215
AciI CCGC 1 cut(s) 354
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 3 cut(s) 221, 334, 443
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 52
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 1 cut(s) 552
AluBI AGCT 7 cut(s) 98, 106, 144, 350, 375, 416, 436
AluI AGCT 7 cut(s) 98, 106, 144, 350, 375, 416, 436
Alw21I GWGCWC 1 cut(s) 146
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 2 cut(s) 57, 340
ApoI RAATTY 3 cut(s) 221, 334, 443
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 1 cut(s) 419
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 1 cut(s) 356
BanII GRGCYC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 146
BceAI ACGGC 1 cut(s) 200
BfaI CTAG 4 cut(s) 243, 372, 395, 568
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 358
BmsI GCATC 1 cut(s) 376
BsaJI CCNNGG 1 cut(s) 322
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse1I ACTGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 3 cut(s) 20, 68, 187
BshFI GGCC 2 cut(s) 59, 342
BsiHKAI GWGCWC 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 342
BsnI GGCC 2 cut(s) 59, 342
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 146
Bsp143I GATC 1 cut(s) 116
Bsp19I CCATGG 1 cut(s) 322
BspACI CCGC 1 cut(s) 354
BspANI GGCC 2 cut(s) 59, 342
BspCNI CTCAG 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 363
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 1 cut(s) 116
BssNAI GTATAC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 322
Bst1107I GTATAC 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 548
Bst6I CTCTTC 1 cut(s) 172
BstC8I GCNNGC 2 cut(s) 352, 414
BstDEI CTNAG 2 cut(s) 94, 120
BstDSI CCRYGG 1 cut(s) 322
BstKTI GATC 1 cut(s) 119
BstMBI GATC 1 cut(s) 116
BstMWI GCNNNNNNNGC 2 cut(s) 150, 422
BstNSI RCATGY 1 cut(s) 319
BstZ17I GTATAC 1 cut(s) 216
BsuRI GGCC 2 cut(s) 59, 342
BtgI CCRYGG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 2 cut(s) 352, 414
Cfr13I GGNCC 1 cut(s) 356
Csp6I GTAC 1 cut(s) 319
CviAII CATG 3 cut(s) 210, 316, 323
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 2 cut(s) 94, 120
DpnI GATC 1 cut(s) 118
DpnII GATC 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
Ecl136II GAGCTC 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 146
Eco32I GATATC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 356
Eco53kI GAGCTC 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 36
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 144
EcoRV GATATC 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 3 cut(s) 213, 319, 326
FaqI GGGAC 1 cut(s) 342
FatI CATG 3 cut(s) 209, 315, 322
FauNDI CATATG 1 cut(s) 22
FblI GTMKAC 1 cut(s) 215
FriOI GRGCYC 1 cut(s) 146
FspBI CTAG 4 cut(s) 243, 372, 395, 568
HaeIII GGCC 2 cut(s) 59, 342
Hin1II CATG 3 cut(s) 213, 319, 326
HindIII AAGCTT 1 cut(s) 104
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 1 cut(s) 395
Hpy8I GTNNAC 1 cut(s) 216
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 7 cut(s) 87, 153, 251, 275, 368, 389, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 150, 422
HpyF3I CTNAG 2 cut(s) 94, 120
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 3 cut(s) 213, 319, 326
Kzo9I GATC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 141, 230
LpnPI CCDG 5 cut(s) 171, 239, 336, 426, 516
LweI GCATC 1 cut(s) 376
MaeI CTAG 4 cut(s) 243, 372, 395, 568
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 1 cut(s) 65
MalI GATC 1 cut(s) 118
MboI GATC 1 cut(s) 116
MboII GAAGA 4 cut(s) 104, 189, 480, 554
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 3 cut(s) 221, 334, 443
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 7 cut(s) 46, 49, 115, 165, 173, 320, 402
MspA1I CMGCKG 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 150, 422
NcoI CCATGG 1 cut(s) 322
NdeI CATATG 1 cut(s) 22
NdeII GATC 1 cut(s) 116
NlaIII CATG 3 cut(s) 213, 319, 326
NlaIV GGNNCC 1 cut(s) 358
NspI RCATGY 1 cut(s) 319
PaeR7I CTCGAG 1 cut(s) 50
PciI ACATGT 1 cut(s) 315
PscI ACATGT 1 cut(s) 315
Psp124BI GAGCTC 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 1 cut(s) 356
PspXI VCTCGAGB 1 cut(s) 50
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacI GAGCTC 1 cut(s) 146
Sau3AI GATC 1 cut(s) 116
Sau96I GGNCC 1 cut(s) 356
SduI GDGCHC 1 cut(s) 146
SfaNI GCATC 1 cut(s) 376
Sfr274I CTCGAG 1 cut(s) 50
SinI GGWCC 1 cut(s) 356
SlaI CTCGAG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 3 cut(s) 221, 334, 443
SsiI CCGC 1 cut(s) 354
SspMI CTAG 4 cut(s) 243, 372, 395, 568
SstI GAGCTC 1 cut(s) 146
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 1 cut(s) 548
TaiI ACGT 1 cut(s) 92
TaqI TCGA 3 cut(s) 51, 115, 480
TasI AATT 3 cut(s) 221, 334, 443
TscAI CASTG 1 cut(s) 165
TspDTI ATGAA 2 cut(s) 352, 414
TspRI CASTG 1 cut(s) 165
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 3 cut(s) 221, 334, 443
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 1 cut(s) 319
XhoI CTCGAG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 215
XspI CTAG 4 cut(s) 243, 372, 395, 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.