FvH4_4g01180

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011743
Physical Location & Seq
Forward (+)
1093608 .. 1096283
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01180.t1

Sequence Viewer

Length: 903 bp
ATGAATCCCCAATCTTTACCTGTGCCAATGGAGAGACTTAATGAGCAATGCCCTCCACCATTTCTCTCCAAAACCTATGAGATTGTGGATGACTCAACAACCAATCACATAGTGTCTTGGAGCAAAGCAAACAACAGCTTTGTTGTATGGGATCCCCAGGCCTTTGCCATCAGGCTTCTCCCCAAGTACTTCAAGCACAACAATTTCTCTAGCTTTGTCAGGCAGCTCAACACTTATGGTCTGGACTCTTGCATTGAGGTTGGAAGTTTCGGATCGTTAGATGGAGAAATTGATCAGTTAAGGCGTGATAAGGAGGTTTTGATGGGGGAACTGGTGAAGCTTAGACAACAGCAGCAGACTACTAGACTTCACCTCCATGGAATGGAAGATAGGCTCAAGAGAACAGAAATGAAACAACAACAGATGATGAATTTCTTGGCAAGAGCAATGCAGAATCCCAACTTTGTACACCAATTGGCACAGAAGAAGGACAGAAAGAAACAACTTGAGGATGCTATTAGAAAGAAAAGAAGGCGGCTTATTGAAGGGCCTAGTAGTATTGAGGTGGGTGAATTAGGCCAAGGTGGAGGAGAAGCTTTTGTTAAAGTTGAACCACAATATTATGGTGACATAACTGAGCTTGAAGTTTCAGGGTTGGACACGTTTGTTGTGGAAATGCAAGGAACAGATGAAAACCAATTGGTCCATGATGGGCTAGAGTGTATGGAGAAAGAAGAGGACCATGAAAGGAGAAGCAAAGATTTGTCTGAGGTATTTTGGGAAGAGTTTTTGAATGACGGCGTTGATGAAGATGTTGAAGTACCAGGTGTTCAAGAAGAGGATGAGGAAGATGTCAATGTGTTGATTGAACAGCTTGGTTGCTTGGTATCCAAGCCCAAGTAA

Protein Analysis

301

Amino Acids

34.6

Weight (kDa)

4.85

Isoelectric Point (pI)

57.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 21 - 83 5.1e-21 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011743)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22830
fragaria_vesca FvH4_4g01180 FvH4_4g01180
malus_domestica MD13G1199700.v1.1 MD16G1199800.v1.1
prunus_persica Prupe.1G021200_v2.0.a1
pyrus_communis pycom13g17330 pycom16g16830
rosa_chinensis RchiOBHm_Chr4g0387751
rosa_laevigata RLG00000010097
rosa_multiflora Rmu_co8508987.1_g000001 Rmu_sc0008916.1_g000004
rosa_roxburghii Rroxscaffold_5G00334390
rosa_rugosa Rorug03G0313000
rosa_samantha Rh4AG018200 Rh4BG013100 Rh4CG019400 Rh4DG014000
rosa_wichuraiana Rw4G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 382
AciI CCGC 1 cut(s) 535
AclWI GGATC 3 cut(s) 146, 159, 280
AcsI RAATTY 1 cut(s) 430
AdeI CACNNNGTG 1 cut(s) 112
AfaI GTAC 3 cut(s) 188, 468, 822
AfiI CCNNNNNNNGG 1 cut(s) 382
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 8 cut(s) 193, 545, 611, 644, 793, 818, 833, 869
AjnI CCWGG 2 cut(s) 156, 823
AluBI AGCT 7 cut(s) 138, 213, 226, 340, 596, 640, 874
AluI AGCT 7 cut(s) 138, 213, 226, 340, 596, 640, 874
Alw26I GTCTC 1 cut(s) 28
AlwI GGATC 3 cut(s) 146, 159, 280
AoxI GGCC 3 cut(s) 159, 548, 577
ApeKI GCWGC 2 cut(s) 223, 352
ApoI RAATTY 1 cut(s) 430
AspS9I GGNCC 3 cut(s) 548, 703, 739
AsuHPI GGTGA 4 cut(s) 346, 362, 581, 638
AvaII GGWCC 2 cut(s) 703, 739
BamHI GGATCC 1 cut(s) 151
BbvI GCAGC 2 cut(s) 235, 364
BccI CCATC 4 cut(s) 176, 275, 316, 704
BceAI ACGGC 1 cut(s) 814
BciT130I CCWGG 2 cut(s) 158, 825
BciVI GTATCC 1 cut(s) 898
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 4 cut(s) 210, 363, 552, 716
BfuI GTATCC 1 cut(s) 898
BisI GCNGC 3 cut(s) 224, 353, 536
BlsI GCNGC 3 cut(s) 225, 354, 537
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 158, 825
Bme18I GGWCC 2 cut(s) 703, 739
BmgT120I GGNCC 3 cut(s) 548, 703, 739
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 158, 825
BmsI GCATC 1 cut(s) 502
BpuEI CTTGAG 2 cut(s) 380, 527
BsaJI CCNNGG 3 cut(s) 156, 376, 580
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 2 cut(s) 53, 453
BseBI CCWGG 2 cut(s) 158, 825
BseDI CCNNGG 3 cut(s) 156, 376, 580
BseGI GGATG 3 cut(s) 94, 517, 847
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMI GCAATG 2 cut(s) 53, 453
BseMII CTCAG 2 cut(s) 627, 759
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 603
BseXI GCAGC 2 cut(s) 235, 364
BshFI GGCC 3 cut(s) 161, 550, 579
BslI CCNNNNNNNGG 1 cut(s) 382
BsmAI GTCTC 1 cut(s) 28
BsnI GGCC 3 cut(s) 161, 550, 579
Bsp1407I TGTACA 1 cut(s) 466
Bsp143I GATC 3 cut(s) 151, 272, 292
Bsp19I CCATGG 1 cut(s) 376
BspACI CCGC 1 cut(s) 535
BspANI GGCC 3 cut(s) 161, 550, 579
BspCNI CTCAG 2 cut(s) 628, 760
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 3 cut(s) 146, 159, 280
BsrDI GCAATG 2 cut(s) 53, 453
BsrGI TGTACA 1 cut(s) 466
BsrI ACTGG 1 cut(s) 336
BssECI CCNNGG 3 cut(s) 156, 376, 580
BssMI GATC 3 cut(s) 151, 272, 292
BssT1I CCWWGG 2 cut(s) 376, 580
Bst2UI CCWGG 2 cut(s) 158, 825
Bst6I CTCTTC 3 cut(s) 729, 777, 831
BstAUI TGTACA 1 cut(s) 466
BstDEI CTNAG 3 cut(s) 341, 636, 768
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 3 cut(s) 94, 517, 847
BstKTI GATC 3 cut(s) 154, 275, 295
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 3 cut(s) 151, 272, 292
BstNI CCWGG 2 cut(s) 158, 825
BstSCI CCNGG 2 cut(s) 156, 823
BstV1I GCAGC 2 cut(s) 235, 364
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuI GTATCC 1 cut(s) 898
BsuRI GGCC 3 cut(s) 161, 550, 579
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 3 cut(s) 94, 517, 847
Cfr13I GGNCC 3 cut(s) 548, 703, 739
CsiI ACCWGGT 1 cut(s) 823
Csp6I GTAC 3 cut(s) 187, 467, 821
CviAII CATG 3 cut(s) 377, 707, 743
CviQI GTAC 3 cut(s) 187, 467, 821
DdeI CTNAG 3 cut(s) 341, 636, 768
DpnI GATC 3 cut(s) 153, 274, 294
DpnII GATC 3 cut(s) 151, 272, 292
DraIII CACNNNGTG 1 cut(s) 112
Eam1104I CTCTTC 3 cut(s) 729, 777, 831
EarI CTCTTC 3 cut(s) 729, 777, 831
Eco130I CCWWGG 2 cut(s) 376, 580
Eco147I AGGCCT 1 cut(s) 161
Eco47I GGWCC 2 cut(s) 703, 739
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 156, 823
EcoT14I CCWWGG 2 cut(s) 376, 580
ErhI CCWWGG 2 cut(s) 376, 580
FaeI CATG 3 cut(s) 380, 710, 746
FatI CATG 3 cut(s) 376, 706, 742
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 3 cut(s) 224, 353, 536
FokI GGATG 3 cut(s) 101, 524, 854
Fsp4HI GCNGC 3 cut(s) 224, 353, 536
FspBI CTAG 4 cut(s) 210, 363, 552, 716
GluI GCNGC 3 cut(s) 224, 353, 536
HaeIII GGCC 3 cut(s) 161, 550, 579
Hin1II CATG 3 cut(s) 380, 710, 746
HindIII AAGCTT 2 cut(s) 338, 594
HinfI GANTC 4 cut(s) 4, 92, 245, 454
HphI GGTGA 4 cut(s) 346, 362, 581, 638
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 2 cut(s) 272, 769
Hpy188III TCNNGA 3 cut(s) 242, 397, 833
Hpy8I GTNNAC 1 cut(s) 469
HpyAV CCTTC 3 cut(s) 481, 525, 539
HpyCH4IV ACGT 1 cut(s) 662
HpyCH4V TGCA 3 cut(s) 252, 451, 679
HpyF3I CTNAG 3 cut(s) 341, 636, 768
HpySE526I ACGT 1 cut(s) 662
Hsp92II CATG 3 cut(s) 380, 710, 746
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 3 cut(s) 151, 272, 292
LmnI GCTCC 1 cut(s) 120
Lsp1109I GCAGC 2 cut(s) 235, 364
LweI GCATC 1 cut(s) 502
MabI ACCWGGT 1 cut(s) 823
MaeI CTAG 4 cut(s) 210, 363, 552, 716
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 1 cut(s) 626
MalI GATC 3 cut(s) 153, 274, 294
MboI GATC 3 cut(s) 151, 272, 292
MboII GAAGA 7 cut(s) 398, 496, 746, 794, 821, 848, 860
MfeI CAATTG 2 cut(s) 473, 698
MflI RGATCY 1 cut(s) 151
MluCI AATT 6 cut(s) 202, 288, 430, 473, 572, 698
MlyI GAGTC 2 cut(s) 86, 239
MmeI TCCRAC 2 cut(s) 241, 636
MseI TTAA 3 cut(s) 39, 299, 603
MslI CAYNNNNRTG 1 cut(s) 375
MspR9I CCNGG 2 cut(s) 158, 825
MunI CAATTG 2 cut(s) 473, 698
MvaI CCWGG 2 cut(s) 158, 825
NcoI CCATGG 1 cut(s) 376
NdeII GATC 3 cut(s) 151, 272, 292
NlaIII CATG 3 cut(s) 380, 710, 746
NlaIV GGNNCC 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 626
PceI AGGCCT 1 cut(s) 161
PfeI GAWTC 2 cut(s) 4, 454
PflMI CCANNNNNTGG 1 cut(s) 382
PkrI GCNGC 3 cut(s) 225, 354, 537
PleI GAGTC 2 cut(s) 86, 239
PpsI GAGTC 2 cut(s) 86, 239
Psp6I CCWGG 2 cut(s) 156, 823
PspGI CCWGG 2 cut(s) 156, 823
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 3 cut(s) 548, 703, 739
PsrI GAACNNNNNNTAC 2 cut(s) 813, 845
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 3 cut(s) 188, 468, 822
RsaNI GTAC 3 cut(s) 187, 467, 821
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 3 cut(s) 39, 299, 603
SatI GCNGC 3 cut(s) 224, 353, 536
Sau3AI GATC 3 cut(s) 151, 272, 292
Sau96I GGNCC 3 cut(s) 548, 703, 739
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 2 cut(s) 86, 239
ScrFI CCNGG 2 cut(s) 158, 825
SexAI ACCWGGT 1 cut(s) 823
SfaNI GCATC 1 cut(s) 502
SinI GGWCC 2 cut(s) 703, 739
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 2 cut(s) 395, 506
SmoI CTYRAG 2 cut(s) 395, 506
Sse9I AATT 6 cut(s) 202, 288, 430, 473, 572, 698
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 1 cut(s) 535
SspI AATATT 1 cut(s) 620
SspMI CTAG 4 cut(s) 210, 363, 552, 716
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 2 cut(s) 156, 823
StyI CCWWGG 2 cut(s) 376, 580
TaiI ACGT 1 cut(s) 665
TasI AATT 6 cut(s) 202, 288, 430, 473, 572, 698
TatI WGTACW 2 cut(s) 186, 466
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 2 cut(s) 4, 454
Tru1I TTAA 3 cut(s) 39, 299, 603
Tru9I TTAA 3 cut(s) 39, 299, 603
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 2 cut(s) 223, 352
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 6 cut(s) 17, 425, 443, 705, 759, 822
Van91I CCANNNNNTGG 1 cut(s) 382
VpaK11BI GGWCC 2 cut(s) 703, 739
XapI RAATTY 1 cut(s) 430
XspI CTAG 4 cut(s) 210, 363, 552, 716
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.