MD13G1199700.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011743
Physical Location & Seq
Forward (+)
17739205 .. 17740848
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1199700.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGAATCTTGATCAAGACGCAGTAAGCTTTTCGTCTTCGAGTGCGGTCGCCGATCATCCTCTACCACTGGAGAGGCTCTATGACCAAGGCCCTCCTCCATTTCTCACCAAAACTTATGACATCGTTGACGACCCATCAACCAATCACATAGTTTCATGGAGTAGAGACAACAACAGCTTTGTTGTTTTGGATCCCAATAGCTTTGCGATGAGTCTTCTCCCCAGATATTTCAAGCATAACAACTTCTCCAGCTTTGTCAGGCAGCTCAACACCTATGGATTTAGGAAGGTTGATACAGATAGATGGGAGTTTGCTAATGAAGCGTTTCTCAGAGGGAAAAAGCATCTTTTAAAGAATATCAGGAGGAGGAAGGCATCCCATCTCCCTCAGTCTTCACAAAAAACTTTGGACTCTTGTGTTGAAGTTGGAAGGTTTGGATTGGACATAGAGATCGATCAGTTGAGGCGCGATAAACAGGTACTAATGGGGGAACTAGTGAAGCTTAGACAGCAACAGCAAGCTACTAGAGTTTACATCCAAGGAATGGAAGACAGACTGAAGAGGACAGAAATGAAACAGCAACATATGTTGAGTTTCTTGGCAAAGGCAATGCAGAATCCCAACTTTGTACAACAATTGTCGCAGCAGAAGGACTTTAGGAAAGAACTTGAGGAAGTGATTACTAAGAAGAGAAGGCGGACTATTGAGCAAGGCCCTAGTAATGTTGAGGTGGATGAATTAGGCCAAGTAGGAGGAGAGACTTTTGTTAAAGTTGAACCGCAAGAATATGGTAATATTTCTAATCAGTTTGAAGTTTTGGAGTTGGACCAATTTGCTATGGATATGCTGGGTGTAAGCGCAATCCAAAACGTCCATGGTGAGGAAGAATGTACAGAGAAAGAAGAGGAACATGAGAGTAGAGGCAAAGAGCTGGATAAGGGGTTTTGGCAAGACTTGTTCAATGAGAGTATTGATGAAGAAATTGGTTTGCTTGGTGTTCAAGAAGAAGATGAGGAAGATGTCGATGTGTTAATTGAGGAGCTTGGTTTCTTGGCTCCCAGTCCCAACTAA

Protein Analysis

357

Amino Acids

41.16

Weight (kDa)

4.91

Isoelectric Point (pI)

60.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 34 - 123 4.2e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011743)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22830
fragaria_vesca FvH4_4g01180 FvH4_4g01180
malus_domestica MD13G1199700.v1.1 MD16G1199800.v1.1
prunus_persica Prupe.1G021200_v2.0.a1
pyrus_communis pycom13g17330 pycom16g16830
rosa_chinensis RchiOBHm_Chr4g0387751
rosa_laevigata RLG00000010097
rosa_multiflora Rmu_co8508987.1_g000001 Rmu_sc0008916.1_g000004
rosa_roxburghii Rroxscaffold_5G00334390
rosa_rugosa Rorug03G0313000
rosa_samantha Rh4AG018200 Rh4BG013100 Rh4CG019400 Rh4DG014000
rosa_wichuraiana Rw4G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 544
AccII CGCG 1 cut(s) 468
AciI CCGC 3 cut(s) 44, 697, 779
AclWI GGATC 2 cut(s) 185, 198
AcuI CTGAAG 1 cut(s) 578
AfaI GTAC 3 cut(s) 480, 630, 892
AfiI CCNNNNNNNGG 2 cut(s) 544, 880
AgsI TTSAA 6 cut(s) 232, 422, 776, 812, 961, 1001
AhlI ACTAGT 1 cut(s) 493
AluBI AGCT 9 cut(s) 27, 177, 201, 252, 265, 502, 521, 931, 1042
AluI AGCT 9 cut(s) 27, 177, 201, 252, 265, 502, 521, 931, 1042
Alw26I GTCTC 2 cut(s) 159, 752
AlwI GGATC 2 cut(s) 185, 198
AoxI GGCC 3 cut(s) 88, 712, 742
ApeKI GCWGC 2 cut(s) 262, 643
Asp700I GAANNNNTTC 1 cut(s) 324
AspLEI GCGC 2 cut(s) 468, 860
AspS9I GGNCC 3 cut(s) 89, 713, 826
AsuHPI GGTGA 2 cut(s) 97, 890
AvaII GGWCC 1 cut(s) 826
BamHI GGATCC 1 cut(s) 190
BbsI GAAGAC 4 cut(s) 27, 206, 384, 555
BbvI GCAGC 2 cut(s) 274, 655
BccI CCATC 3 cut(s) 142, 297, 387
BclI TGATCA 1 cut(s) 10
BcoDI GTCTC 2 cut(s) 159, 752
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 3 cut(s) 494, 525, 717
BisI GCNGC 2 cut(s) 263, 644
BlsI GCNGC 2 cut(s) 264, 645
Bme18I GGWCC 1 cut(s) 826
BmgT120I GGNCC 3 cut(s) 89, 713, 826
BmiI GGNNCC 2 cut(s) 192, 1056
BmrI ACTGGG 1 cut(s) 1053
BmsI GCATC 2 cut(s) 352, 383
BmuI ACTGGG 1 cut(s) 1053
BpiI GAAGAC 4 cut(s) 27, 206, 384, 555
BpmI CTGGAG 2 cut(s) 89, 232
BpuEI CTTGAG 1 cut(s) 689
Bsa29I ATCGAT 1 cut(s) 453
BsaJI CCNNGG 3 cut(s) 85, 538, 874
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 2 cut(s) 544, 880
Bse1I ACTGG 2 cut(s) 72, 1059
Bse3DI GCAATG 1 cut(s) 615
BseCI ATCGAT 1 cut(s) 453
BseDI CCNNGG 3 cut(s) 85, 538, 874
BseGI GGATG 4 cut(s) 55, 374, 534, 739
BseLI CCNNNNNNNGG 2 cut(s) 544, 880
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 2 cut(s) 343, 401
BseNI ACTGG 2 cut(s) 72, 1059
BseRI GAGGAG 4 cut(s) 84, 379, 768, 1052
BseXI GCAGC 2 cut(s) 274, 655
BseYI CCCAGC 1 cut(s) 847
Bsh1236I CGCG 1 cut(s) 468
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 3 cut(s) 90, 714, 744
BshVI ATCGAT 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 1047
BslI CCNNNNNNNGG 2 cut(s) 544, 880
BsmAI GTCTC 2 cut(s) 159, 752
BsmFI GGGAC 1 cut(s) 1047
BsnI GGCC 3 cut(s) 90, 714, 744
Bsp1407I TGTACA 2 cut(s) 628, 890
Bsp143I GATC 5 cut(s) 10, 52, 190, 450, 454
Bsp19I CCATGG 1 cut(s) 874
BspACI CCGC 3 cut(s) 44, 697, 779
BspANI GGCC 3 cut(s) 90, 714, 744
BspCNI CTCAG 2 cut(s) 342, 400
BspDI ATCGAT 1 cut(s) 453
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 2 cut(s) 192, 1056
BspPI GGATC 2 cut(s) 185, 198
BsrDI GCAATG 1 cut(s) 615
BsrGI TGTACA 2 cut(s) 628, 890
BsrI ACTGG 2 cut(s) 72, 1059
BssECI CCNNGG 3 cut(s) 85, 538, 874
BssMI GATC 5 cut(s) 10, 52, 190, 450, 454
BssT1I CCWWGG 3 cut(s) 85, 538, 874
Bst6I CTCTTC 3 cut(s) 554, 683, 897
BstAUI TGTACA 2 cut(s) 628, 890
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 4 cut(s) 329, 387, 503, 684
BstDSI CCRYGG 1 cut(s) 874
BstF5I GGATG 4 cut(s) 55, 374, 534, 739
BstFNI CGCG 1 cut(s) 468
BstHHI GCGC 2 cut(s) 468, 860
BstKTI GATC 5 cut(s) 13, 55, 193, 453, 457
BstMAI GTCTC 2 cut(s) 159, 752
BstMBI GATC 5 cut(s) 10, 52, 190, 450, 454
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 2 cut(s) 320, 508
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 2 cut(s) 274, 655
BstV2I GAAGAC 4 cut(s) 27, 206, 384, 555
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 453
BsuRI GGCC 3 cut(s) 90, 714, 744
BsuTUI ATCGAT 1 cut(s) 453
BtgI CCRYGG 1 cut(s) 874
BtgZI GCGATG 1 cut(s) 221
BtsCI GGATG 4 cut(s) 55, 374, 534, 739
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 2 cut(s) 468, 860
Cfr13I GGNCC 3 cut(s) 89, 713, 826
ClaI ATCGAT 1 cut(s) 453
CseI GACGC 1 cut(s) 26
Csp6I GTAC 3 cut(s) 479, 629, 891
CviAII CATG 3 cut(s) 156, 875, 911
CviQI GTAC 3 cut(s) 479, 629, 891
DdeI CTNAG 4 cut(s) 329, 387, 503, 684
DpnI GATC 5 cut(s) 12, 54, 192, 452, 456
DpnII GATC 5 cut(s) 10, 52, 190, 450, 454
DraI TTTAAA 1 cut(s) 351
Eam1104I CTCTTC 3 cut(s) 554, 683, 897
EarI CTCTTC 3 cut(s) 554, 683, 897
EciI GGCGGA 1 cut(s) 712
Eco130I CCWWGG 3 cut(s) 85, 538, 874
Eco47I GGWCC 1 cut(s) 826
Eco57I CTGAAG 1 cut(s) 578
EcoO109I RGGNCCY 2 cut(s) 89, 713
EcoT14I CCWWGG 3 cut(s) 85, 538, 874
ErhI CCWWGG 3 cut(s) 85, 538, 874
FaeI CATG 3 cut(s) 159, 878, 914
FaqI GGGAC 1 cut(s) 1047
FatI CATG 3 cut(s) 155, 874, 910
FauNDI CATATG 1 cut(s) 585
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 2 cut(s) 263, 644
FokI GGATG 4 cut(s) 42, 361, 521, 746
Fsp4HI GCNGC 2 cut(s) 263, 644
FspBI CTAG 3 cut(s) 494, 525, 717
GlaI GCGC 2 cut(s) 467, 859
GluI GCNGC 2 cut(s) 263, 644
GsaI CCCAGC 1 cut(s) 851
GsuI CTGGAG 2 cut(s) 89, 232
HaeIII GGCC 3 cut(s) 90, 714, 744
HgaI GACGC 1 cut(s) 26
HhaI GCGC 2 cut(s) 468, 860
Hin1II CATG 3 cut(s) 159, 878, 914
Hin6I GCGC 2 cut(s) 466, 858
HinP1I GCGC 2 cut(s) 466, 858
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 2 cut(s) 25, 500
HinfI GANTC 4 cut(s) 4, 211, 410, 616
HphI GGTGA 2 cut(s) 97, 890
Hpy166II GTNNAC 2 cut(s) 127, 532
Hpy188I TCNGA 1 cut(s) 332
Hpy188III TCNNGA 4 cut(s) 8, 14, 361, 1001
Hpy8I GTNNAC 2 cut(s) 127, 532
HpyAV CCTTC 5 cut(s) 280, 364, 423, 643, 687
HpyCH4IV ACGT 1 cut(s) 870
HpyCH4V TGCA 1 cut(s) 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 508
HpyF3I CTNAG 4 cut(s) 329, 387, 503, 684
HpySE526I ACGT 1 cut(s) 870
Hsp92II CATG 3 cut(s) 159, 878, 914
HspAI GCGC 2 cut(s) 466, 858
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 5 cut(s) 10, 52, 190, 450, 454
LmnI GCTCC 2 cut(s) 1039, 1060
LpnPI CCDG 8 cut(s) 53, 235, 244, 262, 346, 461, 833, 917
Lsp1109I GCAGC 2 cut(s) 274, 655
LweI GCATC 2 cut(s) 352, 383
MaeI CTAG 3 cut(s) 494, 525, 717
MaeII ACGT 1 cut(s) 870
MalI GATC 5 cut(s) 12, 54, 192, 452, 456
MboI GATC 5 cut(s) 10, 52, 190, 450, 454
MfeI CAATTG 1 cut(s) 635
MflI RGATCY 1 cut(s) 190
MluCI AATT 5 cut(s) 635, 737, 830, 981, 1032
MlyI GAGTC 2 cut(s) 220, 404
MmeI TCCRAC 2 cut(s) 406, 804
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 3 cut(s) 350, 768, 1031
MunI CAATTG 1 cut(s) 635
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 320, 508
NcoI CCATGG 1 cut(s) 874
NdeI CATATG 1 cut(s) 585
NdeII GATC 5 cut(s) 10, 52, 190, 450, 454
NlaIII CATG 3 cut(s) 159, 878, 914
NlaIV GGNNCC 2 cut(s) 192, 1056
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 2 cut(s) 4, 616
PflMI CCANNNNNTGG 1 cut(s) 544
PkrI GCNGC 2 cut(s) 264, 645
PleI GAGTC 2 cut(s) 219, 404
PpsI GAGTC 2 cut(s) 219, 404
PspFI CCCAGC 1 cut(s) 847
PspN4I GGNNCC 2 cut(s) 192, 1056
PspPI GGNCC 3 cut(s) 89, 713, 826
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 3 cut(s) 480, 630, 892
RsaNI GTAC 3 cut(s) 479, 629, 891
SaqAI TTAA 3 cut(s) 350, 768, 1031
SatI GCNGC 2 cut(s) 263, 644
Sau3AI GATC 5 cut(s) 10, 52, 190, 450, 454
Sau96I GGNCC 3 cut(s) 89, 713, 826
SchI GAGTC 2 cut(s) 220, 404
SfaNI GCATC 2 cut(s) 352, 383
SinI GGWCC 1 cut(s) 826
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 5 cut(s) 635, 737, 830, 981, 1032
SsiI CCGC 3 cut(s) 44, 697, 779
SspI AATATT 1 cut(s) 796
SspMI CTAG 3 cut(s) 494, 525, 717
StyI CCWWGG 3 cut(s) 85, 538, 874
TaiI ACGT 1 cut(s) 873
TaqI TCGA 3 cut(s) 38, 453, 1023
TasI AATT 5 cut(s) 635, 737, 830, 981, 1032
TatI WGTACW 2 cut(s) 628, 890
TfiI GAWTC 2 cut(s) 4, 616
Tru1I TTAA 3 cut(s) 350, 768, 1031
Tru9I TTAA 3 cut(s) 350, 768, 1031
TscAI CASTG 1 cut(s) 72
TseI GCWGC 2 cut(s) 262, 643
TspDTI ATGAA 6 cut(s) 17, 144, 333, 587, 750, 990
TspRI CASTG 1 cut(s) 72
Van91I CCANNNNNTGG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 826
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 3 cut(s) 494, 525, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.