Rorug03G0313000

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011743
Physical Location & Seq
Forward (+)
33539074 .. 33540717
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0313000.1

Sequence Viewer

Length: 951 bp
ATGGAGCCGGAGACCTCAAAAAAAGGTGGTAAGAGAGGAAAAAAGCAACAAAAACGTATAAATGTTGCTGCTATTGCTATGAATAGGCAAGAAAGCAATCCACCAATAAGAATATCGGATGCAGATTCTAATTCTCCTGATATAATATCATCTACTGCAACACCTAATACTGCAAGGTGTGAAGATGAGACGGAACCAGCAAATTCAAAGAGGCCTCCTAGGGGCAAGGCAAAGGGAGATAAAGATTTTAAGAAAAAAGGTCGAGTTGAAGTTGAATTCAATGCTAGGGGTCAGCCTTATGGACAATATGCAGCAGGTTTTTCCTCCTTCCTTGGAGTCACAGCAAGAGAGTTTGTTCCAATTACGCTTAAGAATTGGAAAGACCTCTCAGATACTTATAGAGAACAAATCTGGCAACATATCACGCAACACTATGTGGTTCATGAGTGGTGCAAGAAGCATATTTTTCGAAAAATGGTCAAGATTTGGAGAGACCATAGATCCCTTGTAATGAAAGAAGTGATGGAACAAGCTGAAGTGGTAGGCTTACCACGTGCTGCTGCACAACTCAAACCAGATATTGTAGATTCAATGGAGGAGTGGCAAACTTTCGTTAAATCTAGAAGCACTGAAGAATTCAGGGAAAAGTCAGAAAGATTCAAGTCAATGCGAGAAAAAAAAACTTTATTGCATAGAACAAGTAGAAAAAGTTTTGCCTCCCTTGAGGCAGAGTTGAAGCAACAAAGTGAAACGCCGGATGCAATATCAAGGTCTGATGTTTGGCTTCGTGCTTATGAAGCAAAGCAGAAGAATGGATCAGTTGAGGAAGCTGTCGATTCAGAAATAGTGAAACAAGTGAAAAAGTTCAAGGAAGAAGAAACAGTTTCTGGACCGTCTTCTATAAAGAATGATGCTGTTGCAAAAGTACTCGGTCCAGATCCACGAGGATGA

Protein Analysis

316

Amino Acids

36.09

Weight (kDa)

9.53

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 199 - 316 2.5e-12 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011743)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22830
fragaria_vesca FvH4_4g01180 FvH4_4g01180
malus_domestica MD13G1199700.v1.1 MD16G1199800.v1.1
prunus_persica Prupe.1G021200_v2.0.a1
pyrus_communis pycom13g17330 pycom16g16830
rosa_chinensis RchiOBHm_Chr4g0387751
rosa_laevigata RLG00000010097
rosa_multiflora Rmu_co8508987.1_g000001 Rmu_sc0008916.1_g000004
rosa_roxburghii Rroxscaffold_5G00334390
rosa_rugosa Rorug03G0313000
rosa_samantha Rh4AG018200 Rh4BG013100 Rh4CG019400 Rh4DG014000
rosa_wichuraiana Rw4G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 305
AclWI GGATC 3 cut(s) 495, 823, 932
AcsI RAATTY 3 cut(s) 202, 275, 635
AcuI CTGAAG 2 cut(s) 555, 651
AcvI CACGTG 1 cut(s) 554
AdeI CACNNNGTG 1 cut(s) 436
AfaI GTAC 1 cut(s) 927
AfiI CCNNNNNNNGG 1 cut(s) 221
AflII CTTAAG 1 cut(s) 368
AgsI TTSAA 8 cut(s) 207, 269, 275, 280, 591, 661, 736, 868
AluBI AGCT 2 cut(s) 533, 830
AluI AGCT 2 cut(s) 533, 830
Alw26I GTCTC 3 cut(s) 5, 182, 486
AlwI GGATC 3 cut(s) 495, 823, 932
AlwNI CAGNNNCTG 1 cut(s) 887
AoxI GGCC 1 cut(s) 212
ApeKI GCWGC 4 cut(s) 68, 311, 557, 560
ApoI RAATTY 3 cut(s) 202, 275, 635
Asp700I GAANNNNTTC 1 cut(s) 863
AspA2I CCTAGG 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 890, 932
AsuII TTCGAA 1 cut(s) 469
AvaII GGWCC 2 cut(s) 890, 932
AvrII CCTAGG 1 cut(s) 218
BauI CACGAG 1 cut(s) 942
BbrPI CACGTG 1 cut(s) 554
BbsI GAAGAC 1 cut(s) 888
BbvI GCAGC 4 cut(s) 55, 323, 544, 547
BccI CCATC 1 cut(s) 517
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BcoDI GTCTC 3 cut(s) 5, 182, 486
BfaI CTAG 3 cut(s) 219, 285, 621
BfrI CTTAAG 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 305
BisI GCNGC 4 cut(s) 69, 312, 558, 561
BlnI CCTAGG 1 cut(s) 218
BlsI GCNGC 4 cut(s) 70, 313, 559, 562
BmcAI AGTACT 1 cut(s) 927
Bme18I GGWCC 2 cut(s) 890, 932
BmgT120I GGNCC 2 cut(s) 890, 932
BmiI GGNNCC 2 cut(s) 6, 195
BmsI GCATC 3 cut(s) 109, 748, 901
BpiI GAAGAC 1 cut(s) 888
Bpu14I TTCGAA 1 cut(s) 469
BpuEI CTTGAG 1 cut(s) 743
BsaAI YACGTR 1 cut(s) 554
BsaI GGTCTC 2 cut(s) 5, 486
BsaJI CCNNGG 2 cut(s) 218, 331
Bsc4I CCNNNNNNNGG 1 cut(s) 221
BseDI CCNNGG 2 cut(s) 218, 331
BseGI GGATG 2 cut(s) 124, 763
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMII CTCAG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 611
BseXI GCAGC 4 cut(s) 55, 323, 544, 547
BsgI GTGCAG 1 cut(s) 546
BshFI GGCC 1 cut(s) 214
BsiSI CCGG 2 cut(s) 8, 755
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 3 cut(s) 5, 182, 486
BsmBI CGTCTC 1 cut(s) 182
BsnI GGCC 1 cut(s) 214
Bso31I GGTCTC 2 cut(s) 5, 486
Bsp119I TTCGAA 1 cut(s) 469
Bsp143I GATC 3 cut(s) 500, 815, 937
BspANI GGCC 1 cut(s) 214
BspCNI CTCAG 1 cut(s) 401
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 6, 195
BspMI ACCTGC 1 cut(s) 305
BspPI GGATC 3 cut(s) 495, 823, 932
BspT104I TTCGAA 1 cut(s) 469
BspTI CTTAAG 1 cut(s) 368
BspTNI GGTCTC 2 cut(s) 5, 486
BssECI CCNNGG 2 cut(s) 218, 331
BssMI GATC 3 cut(s) 500, 815, 937
BssSI CACGAG 1 cut(s) 942
BssT1I CCWWGG 2 cut(s) 218, 331
Bst2BI CACGAG 1 cut(s) 942
Bst4CI ACNGT 2 cut(s) 883, 894
BstAFI CTTAAG 1 cut(s) 368
BstBAI YACGTR 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 469
BstDEI CTNAG 1 cut(s) 388
BstF5I GGATG 2 cut(s) 124, 763
BstKTI GATC 3 cut(s) 503, 818, 940
BstMAI GTCTC 3 cut(s) 5, 182, 486
BstMBI GATC 3 cut(s) 500, 815, 937
BstMWI GCNNNNNNNGC 2 cut(s) 74, 797
BstV1I GCAGC 4 cut(s) 55, 323, 544, 547
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 2 cut(s) 500, 937
BstYI RGATCY 2 cut(s) 500, 937
BsuRI GGCC 1 cut(s) 214
BtsCI GGATG 2 cut(s) 124, 763
BtsIMutI CAGTG 1 cut(s) 627
BveI ACCTGC 1 cut(s) 305
CaiI CAGNNNCTG 1 cut(s) 887
CciI TCATGA 1 cut(s) 442
Cfr13I GGNCC 2 cut(s) 890, 932
Csp6I GTAC 1 cut(s) 926
CviAII CATG 1 cut(s) 443
CviJI RGCY 7 cut(s) 7, 214, 295, 533, 546, 784, 830
CviKI_1 RGCY 7 cut(s) 7, 214, 295, 533, 546, 784, 830
CviQI GTAC 1 cut(s) 926
DdeI CTNAG 1 cut(s) 388
DpnI GATC 3 cut(s) 502, 817, 939
DpnII GATC 3 cut(s) 500, 815, 937
DraIII CACNNNGTG 1 cut(s) 436
Eco130I CCWWGG 2 cut(s) 218, 331
Eco147I AGGCCT 1 cut(s) 214
Eco31I GGTCTC 2 cut(s) 5, 486
Eco47I GGWCC 2 cut(s) 890, 932
Eco57I CTGAAG 2 cut(s) 555, 651
Eco72I CACGTG 1 cut(s) 554
EcoRI GAATTC 2 cut(s) 275, 635
EcoT14I CCWWGG 2 cut(s) 218, 331
ErhI CCWWGG 2 cut(s) 218, 331
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 1 cut(s) 446
FatI CATG 1 cut(s) 442
Fnu4HI GCNGC 4 cut(s) 69, 312, 558, 561
FokI GGATG 2 cut(s) 131, 770
Fsp4HI GCNGC 4 cut(s) 69, 312, 558, 561
FspBI CTAG 3 cut(s) 219, 285, 621
GluI GCNGC 4 cut(s) 69, 312, 558, 561
HaeIII GGCC 1 cut(s) 214
HapII CCGG 2 cut(s) 8, 755
Hin1II CATG 1 cut(s) 446
HinfI GANTC 5 cut(s) 125, 336, 587, 657, 836
HpaII CCGG 2 cut(s) 8, 755
Hpy188I TCNGA 5 cut(s) 118, 391, 652, 775, 841
Hpy188III TCNNGA 6 cut(s) 137, 443, 481, 621, 888, 935
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 2 cut(s) 883, 894
HpyCH4IV ACGT 2 cut(s) 55, 553
HpyCH4V TGCA 9 cut(s) 122, 158, 173, 311, 453, 563, 691, 761, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 797
HpyF3I CTNAG 1 cut(s) 388
HpySE526I ACGT 2 cut(s) 55, 553
Hsp92II CATG 1 cut(s) 446
Kzo9I GATC 3 cut(s) 500, 815, 937
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 21, 150, 210, 300, 397, 588, 625, 768, 873
Lsp1109I GCAGC 4 cut(s) 55, 323, 544, 547
LweI GCATC 3 cut(s) 109, 748, 901
MaeI CTAG 3 cut(s) 219, 285, 621
MaeII ACGT 2 cut(s) 55, 553
MaeIII GTNAC 1 cut(s) 337
MalI GATC 3 cut(s) 502, 817, 939
MboI GATC 3 cut(s) 500, 815, 937
MboII GAAGA 6 cut(s) 194, 644, 820, 884, 887, 888
MflI RGATCY 2 cut(s) 500, 937
MluCI AATT 6 cut(s) 130, 202, 275, 360, 373, 635
MlyI GAGTC 1 cut(s) 345
MroXI GAANNNNTTC 1 cut(s) 863
MseI TTAA 3 cut(s) 249, 369, 615
MslI CAYNNNNRTG 1 cut(s) 946
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 2 cut(s) 8, 755
MwoI GCNNNNNNNGC 2 cut(s) 74, 797
NdeII GATC 3 cut(s) 500, 815, 937
NlaIII CATG 1 cut(s) 446
NlaIV GGNNCC 2 cut(s) 6, 195
NmuCI GTSAC 1 cut(s) 337
NspV TTCGAA 1 cut(s) 469
PagI TCATGA 1 cut(s) 442
PceI AGGCCT 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 863
PfeI GAWTC 4 cut(s) 125, 587, 657, 836
PkrI GCNGC 4 cut(s) 70, 313, 559, 562
PleI GAGTC 1 cut(s) 344
PmaCI CACGTG 1 cut(s) 554
PmlI CACGTG 1 cut(s) 554
PpsI GAGTC 1 cut(s) 344
Ppu21I YACGTR 1 cut(s) 554
PspCI CACGTG 1 cut(s) 554
PspN4I GGNNCC 2 cut(s) 6, 195
PspPI GGNCC 2 cut(s) 890, 932
PstNI CAGNNNCTG 1 cut(s) 887
PsuI RGATCY 2 cut(s) 500, 937
RsaI GTAC 1 cut(s) 927
RsaNI GTAC 1 cut(s) 926
RseI CAYNNNNRTG 1 cut(s) 946
SaqAI TTAA 3 cut(s) 249, 369, 615
SatI GCNGC 4 cut(s) 69, 312, 558, 561
Sau3AI GATC 3 cut(s) 500, 815, 937
Sau96I GGNCC 2 cut(s) 890, 932
ScaI AGTACT 1 cut(s) 927
SchI GAGTC 1 cut(s) 345
SfaNI GCATC 3 cut(s) 109, 748, 901
SfuI TTCGAA 1 cut(s) 469
SinI GGWCC 2 cut(s) 890, 932
SmiMI CAYNNNNRTG 1 cut(s) 946
SmlI CTYRAG 2 cut(s) 368, 722
SmoI CTYRAG 2 cut(s) 368, 722
Sse9I AATT 6 cut(s) 130, 202, 275, 360, 373, 635
SseBI AGGCCT 1 cut(s) 214
SspMI CTAG 3 cut(s) 219, 285, 621
StuI AGGCCT 1 cut(s) 214
StyI CCWWGG 2 cut(s) 218, 331
TaaI ACNGT 2 cut(s) 883, 894
TaiI ACGT 2 cut(s) 58, 556
TaqI TCGA 3 cut(s) 262, 469, 834
TaqII GACCGA 1 cut(s) 920
TasI AATT 6 cut(s) 130, 202, 275, 360, 373, 635
TatI WGTACW 1 cut(s) 925
TfiI GAWTC 4 cut(s) 125, 587, 657, 836
Tru1I TTAA 3 cut(s) 249, 369, 615
Tru9I TTAA 3 cut(s) 249, 369, 615
TscAI CASTG 1 cut(s) 634
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 4 cut(s) 68, 311, 557, 560
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 4 cut(s) 95, 431, 527, 810
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 1 cut(s) 634
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 2 cut(s) 890, 932
XapI RAATTY 3 cut(s) 202, 275, 635
XbaI TCTAGA 1 cut(s) 620
XmaJI CCTAGG 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 863
XspI CTAG 3 cut(s) 219, 285, 621
ZrmI AGTACT 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.