Rh4DG014000

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011743
Physical Location & Seq
Forward (+)
2148421 .. 2234688
86268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG014000.1

Sequence Viewer

Length: 1026 bp
ATGAACCCCCAAGGTCAAGTAAAGGAGGCACCTTTACCTGTGCCAATGGAGAGACTTAATGACCAAGGCCCTCCCCCATTTCTCAACAAAACCTATGAAATTGTGGAAGACTCAACAACCAACCACATAGTGTCTTGGAGCAAAGCAAACAACAGCTTTGTTGTATGGGATCCCCAGGCCTTTGCCATCAGTCTTCTCCCCAAATACTTCAAGCACGGCAATTTCTCTAGCTTTGTCAGGCAGCTCAACACCTATGGATTTAGAAAGGTTGACACAGATAAATGGGAGTTTGCTCATGAAGTGTTTCTCAGAGGGCAAAAGCATCTTTTAAAGAATATCAGGAGAAGGAGGACATCTCATCATTATGGTTCGAAGCAAGATCTGGACTCTTGCATTGAGGTTGGAAGTTTCGGATCATTAGATGGAGAGATTGATCAGTTGAGGCGCGATAAGGAGGTTTTGATGGGGGAACTTGTGAAGCTTAGACAACAGCAGCAGACTGCTAGACTTCACCTCCATGGAATGGAAGATAGGCTCAAGAGGACAGAGATGAAACAACAACAGATGATGAATTTCTTGGCAAGAGCAATGCAGAATCCCAACTTTATACAACAATTGGTACAAAAGAAGGAAAGAAAGAAACAACTTGAGGAAGCAATTACGAAGAAGAGAAGGCGGTCTATTGAGGTGGGTGTATTAGGCCAAGGTGGAGGAGAAGCTGTTGTTAAAGTTGAACCCGAATATTATGGTGACATATCTGAGCTGGATGTTTCAGGGTTAGACAACTTTGCTATGGACATGCCAGTGACAGATGAAAATGAAAAGGTCCATGATGGGGAAGAGTGTATGGAGAAAGAAGAGGAACATGAAAGGAGAGGCAAAGATCTATCTGAGGTATTTTGGAAAGAGTTTTTGAATGCAGGCGTTGATGAAGAAATTGAAGTACTCGGTGTTCAAGAAGGAGATGAGGAAGATGTCAGTGTGTTGATTGAGGAGCTTGGTTGCTTGGTGTCCAAGCCCGAGTAA

Protein Analysis

341

Amino Acids

39.39

Weight (kDa)

5.33

Isoelectric Point (pI)

58.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 27 - 116 5.9e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011743)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22830
fragaria_vesca FvH4_4g01180 FvH4_4g01180
malus_domestica MD13G1199700.v1.1 MD16G1199800.v1.1
prunus_persica Prupe.1G021200_v2.0.a1
pyrus_communis pycom13g17330 pycom16g16830
rosa_chinensis RchiOBHm_Chr4g0387751
rosa_laevigata RLG00000010097
rosa_multiflora Rmu_co8508987.1_g000001 Rmu_sc0008916.1_g000004
rosa_roxburghii Rroxscaffold_5G00334390
rosa_rugosa Rorug03G0313000
rosa_samantha Rh4AG018200 Rh4BG013100 Rh4CG019400 Rh4DG014000
rosa_wichuraiana Rw4G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 28
AccB7I CCANNNNNTGG 1 cut(s) 523
AccII CGCG 1 cut(s) 447
AciI CCGC 1 cut(s) 676
AclWI GGATC 3 cut(s) 164, 177, 421
AcsI RAATTY 1 cut(s) 571
AdeI CACNNNGTG 1 cut(s) 130
AfaI GTAC 2 cut(s) 621, 945
AfiI CCNNNNNNNGG 2 cut(s) 523, 835
AgsI TTSAA 5 cut(s) 211, 734, 916, 941, 956
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 7 cut(s) 156, 231, 244, 481, 719, 763, 997
AluI AGCT 7 cut(s) 156, 231, 244, 481, 719, 763, 997
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 3 cut(s) 164, 177, 421
Ama87I CYCGRG 1 cut(s) 1019
AoxI GGCC 3 cut(s) 67, 177, 700
ApeKI GCWGC 2 cut(s) 241, 493
ApoI RAATTY 1 cut(s) 571
Asp700I GAANNNNTTC 1 cut(s) 303
AspLEI GCGC 1 cut(s) 447
AspS9I GGNCC 2 cut(s) 68, 826
AsuHPI GGTGA 2 cut(s) 503, 761
AsuII TTCGAA 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 1019
AvaII GGWCC 1 cut(s) 826
BamHI GGATCC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 28
BbsI GAAGAC 2 cut(s) 114, 185
BbvI GCAGC 2 cut(s) 253, 505
BccI CCATC 4 cut(s) 194, 416, 457, 827
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 1 cut(s) 176
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 2 cut(s) 228, 504
BglII AGATCT 2 cut(s) 379, 883
BisI GCNGC 2 cut(s) 242, 494
BlsI GCNGC 2 cut(s) 243, 495
BmcAI AGTACT 1 cut(s) 945
Bme1390I CCNGG 1 cut(s) 176
Bme18I GGWCC 1 cut(s) 826
BmeT110I CYCGRG 1 cut(s) 1019
BmgT120I GGNCC 2 cut(s) 68, 826
BmiI GGNNCC 2 cut(s) 30, 171
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 1 cut(s) 331
BpiI GAAGAC 2 cut(s) 114, 185
BplI GAGNNNNNCTC 2 cut(s) 340, 372
Bpu14I TTCGAA 1 cut(s) 371
BpuEI CTTGAG 2 cut(s) 521, 668
BsaJI CCNNGG 5 cut(s) 10, 64, 174, 517, 703
BsaXI ACNNNNNCTCC 4 cut(s) 498, 528, 705, 735
Bsc4I CCNNNNNNNGG 2 cut(s) 523, 835
Bse1I ACTGG 1 cut(s) 803
Bse3DI GCAATG 1 cut(s) 594
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 5 cut(s) 10, 64, 174, 517, 703
BseGI GGATG 1 cut(s) 772
BseLI CCNNNNNNNGG 2 cut(s) 523, 835
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 3 cut(s) 322, 750, 882
BseNI ACTGG 1 cut(s) 803
BseRI GAGGAG 2 cut(s) 726, 1007
BseXI GCAGC 2 cut(s) 253, 505
Bsh1236I CGCG 1 cut(s) 447
BshFI GGCC 3 cut(s) 69, 179, 702
BshNI GGYRCC 1 cut(s) 28
BsiHKCI CYCGRG 1 cut(s) 1019
BslI CCNNNNNNNGG 2 cut(s) 523, 835
BsmAI GTCTC 1 cut(s) 46
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 3 cut(s) 69, 179, 702
BsoBI CYCGRG 1 cut(s) 1019
Bsp119I TTCGAA 1 cut(s) 371
Bsp143I GATC 5 cut(s) 169, 379, 413, 433, 883
Bsp19I CCATGG 1 cut(s) 517
BspACI CCGC 1 cut(s) 676
BspANI GGCC 3 cut(s) 69, 179, 702
BspCNI CTCAG 3 cut(s) 321, 751, 883
BspFNI CGCG 1 cut(s) 447
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 30, 171
BspPI GGATC 3 cut(s) 164, 177, 421
BspT104I TTCGAA 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 1 cut(s) 803
BssECI CCNNGG 5 cut(s) 10, 64, 174, 517, 703
BssMI GATC 5 cut(s) 169, 379, 413, 433, 883
BssT1I CCWWGG 4 cut(s) 10, 64, 517, 703
Bst2UI CCWGG 1 cut(s) 176
Bst6I CTCTTC 3 cut(s) 662, 834, 852
BstBI TTCGAA 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 922
BstDEI CTNAG 4 cut(s) 308, 482, 759, 891
BstDSI CCRYGG 1 cut(s) 517
BstF5I GGATG 1 cut(s) 772
BstFNI CGCG 1 cut(s) 447
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 5 cut(s) 172, 382, 416, 436, 886
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 5 cut(s) 169, 379, 413, 433, 883
BstNI CCWGG 1 cut(s) 176
BstNSI RCATGY 1 cut(s) 802
BstSCI CCNGG 1 cut(s) 174
BstUI CGCG 1 cut(s) 447
BstV1I GCAGC 2 cut(s) 253, 505
BstV2I GAAGAC 2 cut(s) 114, 185
BstX2I RGATCY 3 cut(s) 169, 379, 883
BstYI RGATCY 3 cut(s) 169, 379, 883
BsuRI GGCC 3 cut(s) 69, 179, 702
BtgI CCRYGG 1 cut(s) 517
BtsCI GGATG 1 cut(s) 772
BtsIMutI CAGTG 2 cut(s) 810, 985
Cac8I GCNNGC 1 cut(s) 922
CciI TCATGA 1 cut(s) 295
CfoI GCGC 1 cut(s) 447
Cfr13I GGNCC 2 cut(s) 68, 826
Csp6I GTAC 2 cut(s) 620, 944
CviAII CATG 5 cut(s) 296, 518, 799, 830, 866
CviQI GTAC 2 cut(s) 620, 944
DdeI CTNAG 4 cut(s) 308, 482, 759, 891
DpnI GATC 5 cut(s) 171, 381, 415, 435, 885
DpnII GATC 5 cut(s) 169, 379, 413, 433, 883
DraI TTTAAA 1 cut(s) 330
DraIII CACNNNGTG 1 cut(s) 130
Eam1104I CTCTTC 3 cut(s) 662, 834, 852
EarI CTCTTC 3 cut(s) 662, 834, 852
Eco130I CCWWGG 4 cut(s) 10, 64, 517, 703
Eco147I AGGCCT 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 826
Eco88I CYCGRG 1 cut(s) 1019
EcoO109I RGGNCCY 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 4 cut(s) 10, 64, 517, 703
ErhI CCWWGG 4 cut(s) 10, 64, 517, 703
FaeI CATG 5 cut(s) 299, 521, 802, 833, 869
FatI CATG 5 cut(s) 295, 517, 798, 829, 865
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 2 cut(s) 242, 494
FokI GGATG 1 cut(s) 779
Fsp4HI GCNGC 2 cut(s) 242, 494
FspBI CTAG 2 cut(s) 228, 504
GlaI GCGC 1 cut(s) 446
GluI GCNGC 2 cut(s) 242, 494
HaeIII GGCC 3 cut(s) 69, 179, 702
HhaI GCGC 1 cut(s) 447
Hin1II CATG 5 cut(s) 299, 521, 802, 833, 869
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 3 cut(s) 110, 386, 595
HphI GGTGA 2 cut(s) 503, 761
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 4 cut(s) 311, 413, 760, 892
Hpy188III TCNNGA 5 cut(s) 296, 340, 383, 538, 956
Hpy8I GTNNAC 1 cut(s) 271
HpyAV CCTTC 4 cut(s) 339, 622, 666, 953
HpyCH4V TGCA 3 cut(s) 393, 592, 920
HpyF3I CTNAG 4 cut(s) 308, 482, 759, 891
Hsp92II CATG 5 cut(s) 299, 521, 802, 833, 869
HspAI GCGC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 5 cut(s) 169, 379, 413, 433, 883
LmnI GCTCC 2 cut(s) 138, 994
Lsp1109I GCAGC 2 cut(s) 253, 505
LweI GCATC 1 cut(s) 331
MaeI CTAG 2 cut(s) 228, 504
MaeIII GTNAC 2 cut(s) 749, 805
MalI GATC 5 cut(s) 171, 381, 415, 435, 885
MboI GATC 5 cut(s) 169, 379, 413, 433, 883
MboII GAAGA 9 cut(s) 119, 185, 539, 676, 679, 851, 869, 944, 983
MfeI CAATTG 1 cut(s) 614
MflI RGATCY 3 cut(s) 169, 379, 883
MluCI AATT 6 cut(s) 99, 220, 571, 614, 657, 936
MlyI GAGTC 2 cut(s) 104, 380
MmeI TCCRAC 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 3 cut(s) 57, 329, 726
MslI CAYNNNNRTG 3 cut(s) 363, 516, 803
MspR9I CCNGG 1 cut(s) 176
MunI CAATTG 1 cut(s) 614
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 1 cut(s) 176
MvnI CGCG 1 cut(s) 447
NcoI CCATGG 1 cut(s) 517
NdeII GATC 5 cut(s) 169, 379, 413, 433, 883
NlaIII CATG 5 cut(s) 299, 521, 802, 833, 869
NlaIV GGNNCC 2 cut(s) 30, 171
NmuCI GTSAC 2 cut(s) 749, 805
NspI RCATGY 1 cut(s) 802
NspV TTCGAA 1 cut(s) 371
PagI TCATGA 1 cut(s) 295
PceI AGGCCT 1 cut(s) 179
PctI GAATGC 1 cut(s) 922
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 595
PflMI CCANNNNNTGG 1 cut(s) 523
PkrI GCNGC 2 cut(s) 243, 495
PleI GAGTC 2 cut(s) 104, 380
PpsI GAGTC 2 cut(s) 104, 380
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 2 cut(s) 30, 171
PspPI GGNCC 2 cut(s) 68, 826
PsrI GAACNNNNNNTAC 2 cut(s) 936, 968
PsuI RGATCY 3 cut(s) 169, 379, 883
RsaI GTAC 2 cut(s) 621, 945
RsaNI GTAC 2 cut(s) 620, 944
RseI CAYNNNNRTG 3 cut(s) 363, 516, 803
SaqAI TTAA 3 cut(s) 57, 329, 726
SatI GCNGC 2 cut(s) 242, 494
Sau3AI GATC 5 cut(s) 169, 379, 413, 433, 883
Sau96I GGNCC 2 cut(s) 68, 826
ScaI AGTACT 1 cut(s) 945
SchI GAGTC 2 cut(s) 104, 380
ScrFI CCNGG 1 cut(s) 176
SfaNI GCATC 1 cut(s) 331
SfuI TTCGAA 1 cut(s) 371
SinI GGWCC 1 cut(s) 826
SmiMI CAYNNNNRTG 3 cut(s) 363, 516, 803
SmlI CTYRAG 2 cut(s) 536, 647
SmoI CTYRAG 2 cut(s) 536, 647
Sse9I AATT 6 cut(s) 99, 220, 571, 614, 657, 936
SseBI AGGCCT 1 cut(s) 179
SsiI CCGC 1 cut(s) 676
SspI AATATT 1 cut(s) 743
SspMI CTAG 2 cut(s) 228, 504
StuI AGGCCT 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 174
StyI CCWWGG 4 cut(s) 10, 64, 517, 703
TaqI TCGA 1 cut(s) 371
TasI AATT 6 cut(s) 99, 220, 571, 614, 657, 936
TatI WGTACW 1 cut(s) 943
TfiI GAWTC 1 cut(s) 595
Tru1I TTAA 3 cut(s) 57, 329, 726
Tru9I TTAA 3 cut(s) 57, 329, 726
TscAI CASTG 2 cut(s) 810, 985
TseFI GTSAC 2 cut(s) 749, 805
TseI GCWGC 2 cut(s) 241, 493
Tsp45I GTSAC 2 cut(s) 749, 805
TspDTI ATGAA 9 cut(s) 17, 111, 312, 566, 584, 828, 834, 882, 945
TspRI CASTG 2 cut(s) 810, 985
Van91I CCANNNNNTGG 1 cut(s) 523
VpaK11BI GGWCC 1 cut(s) 826
XapI RAATTY 1 cut(s) 571
XceI RCATGY 1 cut(s) 802
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 2 cut(s) 228, 504
ZrmI AGTACT 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.