Rh4CG019400

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0011743
Physical Location & Seq
Forward (+)
3774456 .. 3776486
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG019400.1

Sequence Viewer

Length: 1056 bp
ATGAACCCCCAAGGTAGTCAAGTAAAGGAGGCACCTTTACCTGTGCCACTGGAGAGACTTAATGACCAAGGCCCTCCCCCATTTCTCAACAAAACCTATGAAATTGTGGAAGACTCAACAACCAACCACATAGTGTCTTGGAGCAAAGCAAACAACAGCTTTGTTGTATGGGATCCCCAGGCCTTTGCCATCAGTCTTCTCCCCAAATACTTCAAGCACGGCAATTTCTCTAGCTTTGTCAGGCAGCTCAACACCTATGGATTTAGAAAGGTTGACACAGATAAATGGGAGTTTGCTCATGAAGTGTTTCTCAGAGGGCAAAAGCATCTTTTAAAGAATATCAGGAGGAGGAGGACATCTCATCATAATGGTCATTATGGTTCGAAGCAAGATCTGGACTCTTGCATTGAGGTTGGAAGTTTCGGATCATTAGATGGAGAGATTGATCAGTTGAGGCGCGATAAGGAGGTTTTGATGGGGGAACTGGTGAAGCTTAGACAACAGCAGCAGACTGCTAGACTTCACCTCCATGGAATGGAAGATAGGCTCAAGAGGACAGAGATGAAACAACAACAGATGATGAGTTTCTTGGCAAGAGCAATGCAGAATCCCAACTTTTTACAACAATTGGTACAGAAGAAGGAAAGAAAGAAACAACTTGAGGAAGCAATTACGAAGAAGAGAAGGCGGACTATTGAAGGGCCTAGTAGTATTGAGGTGGGTGTATTAGGCCAAGGTGGAGGAGAAGCTGTTGTTAAAGTTGAACCCGAATATTATGGTGACATATCTGAGCTGGATGTTTCAGGGTTAGACAACTTTGCTATGGACATGCCAGTGACAGATGAAAACGAAAAGGTCCATGATGGGGAAGAGTGTATGGAGACAGAAGAGGAATATGAAAGGAGAGGCAAAGATCTGTCTGAGGTATTTTGGAAAGAGTTTTTGAATGAAGGCGTTGATGAAGAAATTGAAGTACTCGGTGTTCAAGAAGAAGATGAGGAAGATGTCAGTGTGTTGATTGAGCAGCTTGGTTGCTTGGTGTCCAAGCCCAAGTAA

Protein Analysis

351

Amino Acids

40.45

Weight (kDa)

5.27

Isoelectric Point (pI)

62.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 28 - 117 6.2e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011743)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22830
fragaria_vesca FvH4_4g01180 FvH4_4g01180
malus_domestica MD13G1199700.v1.1 MD16G1199800.v1.1
prunus_persica Prupe.1G021200_v2.0.a1
pyrus_communis pycom13g17330 pycom16g16830
rosa_chinensis RchiOBHm_Chr4g0387751
rosa_laevigata RLG00000010097
rosa_multiflora Rmu_co8508987.1_g000001 Rmu_sc0008916.1_g000004
rosa_roxburghii Rroxscaffold_5G00334390
rosa_rugosa Rorug03G0313000
rosa_samantha Rh4AG018200 Rh4BG013100 Rh4CG019400 Rh4DG014000
rosa_wichuraiana Rw4G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 535
AccII CGCG 1 cut(s) 459
AciI CCGC 1 cut(s) 688
AclWI GGATC 3 cut(s) 167, 180, 433
AdeI CACNNNGTG 1 cut(s) 133
AfaI GTAC 2 cut(s) 633, 975
AfiI CCNNNNNNNGG 2 cut(s) 535, 865
AgsI TTSAA 6 cut(s) 214, 698, 764, 946, 971, 986
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 7 cut(s) 159, 234, 247, 493, 749, 793, 1027
AluI AGCT 7 cut(s) 159, 234, 247, 493, 749, 793, 1027
Alw26I GTCTC 2 cut(s) 49, 875
AlwI GGATC 3 cut(s) 167, 180, 433
AoxI GGCC 4 cut(s) 70, 180, 701, 730
ApeKI GCWGC 3 cut(s) 244, 505, 1024
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 3 cut(s) 71, 701, 856
AsuHPI GGTGA 3 cut(s) 499, 515, 791
AsuII TTCGAA 1 cut(s) 383
AvaII GGWCC 1 cut(s) 856
BamHI GGATCC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 117, 188
BbvI GCAGC 3 cut(s) 256, 517, 1036
BccI CCATC 4 cut(s) 197, 428, 469, 857
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 179
BclI TGATCA 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 49, 875
BfaI CTAG 3 cut(s) 231, 516, 705
BglII AGATCT 2 cut(s) 391, 913
BisI GCNGC 3 cut(s) 245, 506, 1025
BlsI GCNGC 3 cut(s) 246, 507, 1026
BmcAI AGTACT 1 cut(s) 975
Bme1390I CCNGG 1 cut(s) 179
Bme18I GGWCC 1 cut(s) 856
BmgT120I GGNCC 3 cut(s) 71, 701, 856
BmiI GGNNCC 2 cut(s) 33, 174
BmrFI CCNGG 1 cut(s) 179
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 2 cut(s) 117, 188
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpmI CTGGAG 1 cut(s) 71
Bpu14I TTCGAA 1 cut(s) 383
BpuEI CTTGAG 2 cut(s) 533, 680
BsaJI CCNNGG 5 cut(s) 10, 67, 177, 529, 733
BsaXI ACNNNNNCTCC 4 cut(s) 510, 540, 735, 765
Bsc4I CCNNNNNNNGG 2 cut(s) 535, 865
Bse1I ACTGG 3 cut(s) 54, 489, 833
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 5 cut(s) 10, 67, 177, 529, 733
BseGI GGATG 1 cut(s) 802
BseLI CCNNNNNNNGG 2 cut(s) 535, 865
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 3 cut(s) 325, 780, 912
BseNI ACTGG 3 cut(s) 54, 489, 833
BseRI GAGGAG 3 cut(s) 361, 364, 756
BseXI GCAGC 3 cut(s) 256, 517, 1036
Bsh1236I CGCG 1 cut(s) 459
BshFI GGCC 4 cut(s) 72, 182, 703, 732
BshNI GGYRCC 1 cut(s) 31
BslI CCNNNNNNNGG 2 cut(s) 535, 865
BsmAI GTCTC 2 cut(s) 49, 875
BsnI GGCC 4 cut(s) 72, 182, 703, 732
Bsp119I TTCGAA 1 cut(s) 383
Bsp143I GATC 5 cut(s) 172, 391, 425, 445, 913
Bsp19I CCATGG 1 cut(s) 529
BspACI CCGC 1 cut(s) 688
BspANI GGCC 4 cut(s) 72, 182, 703, 732
BspCNI CTCAG 3 cut(s) 324, 781, 913
BspFNI CGCG 1 cut(s) 459
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 33, 174
BspPI GGATC 3 cut(s) 167, 180, 433
BspT104I TTCGAA 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 606
BsrI ACTGG 3 cut(s) 54, 489, 833
BssECI CCNNGG 5 cut(s) 10, 67, 177, 529, 733
BssMI GATC 5 cut(s) 172, 391, 425, 445, 913
BssT1I CCWWGG 4 cut(s) 10, 67, 529, 733
Bst2UI CCWGG 1 cut(s) 179
Bst6I CTCTTC 3 cut(s) 674, 864, 882
BstBI TTCGAA 1 cut(s) 383
BstDEI CTNAG 4 cut(s) 311, 494, 789, 921
BstDSI CCRYGG 1 cut(s) 529
BstF5I GGATG 1 cut(s) 802
BstFNI CGCG 1 cut(s) 459
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 5 cut(s) 175, 394, 428, 448, 916
BstMAI GTCTC 2 cut(s) 49, 875
BstMBI GATC 5 cut(s) 172, 391, 425, 445, 913
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 1 cut(s) 832
BstSCI CCNGG 1 cut(s) 177
BstUI CGCG 1 cut(s) 459
BstV1I GCAGC 3 cut(s) 256, 517, 1036
BstV2I GAAGAC 2 cut(s) 117, 188
BstX2I RGATCY 3 cut(s) 172, 391, 913
BstYI RGATCY 3 cut(s) 172, 391, 913
BsuRI GGCC 4 cut(s) 72, 182, 703, 732
BtgI CCRYGG 1 cut(s) 529
BtsCI GGATG 1 cut(s) 802
BtsIMutI CAGTG 3 cut(s) 47, 840, 1015
CciI TCATGA 1 cut(s) 298
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 3 cut(s) 71, 701, 856
Csp6I GTAC 2 cut(s) 632, 974
CviAII CATG 4 cut(s) 299, 530, 829, 860
CviQI GTAC 2 cut(s) 632, 974
DdeI CTNAG 4 cut(s) 311, 494, 789, 921
DpnI GATC 5 cut(s) 174, 393, 427, 447, 915
DpnII GATC 5 cut(s) 172, 391, 425, 445, 913
DraI TTTAAA 1 cut(s) 333
DraIII CACNNNGTG 1 cut(s) 133
Eam1104I CTCTTC 3 cut(s) 674, 864, 882
EarI CTCTTC 3 cut(s) 674, 864, 882
EciI GGCGGA 1 cut(s) 703
Eco130I CCWWGG 4 cut(s) 10, 67, 529, 733
Eco147I AGGCCT 1 cut(s) 182
Eco47I GGWCC 1 cut(s) 856
EcoO109I RGGNCCY 2 cut(s) 71, 701
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 4 cut(s) 10, 67, 529, 733
ErhI CCWWGG 4 cut(s) 10, 67, 529, 733
FaeI CATG 4 cut(s) 302, 533, 832, 863
FatI CATG 4 cut(s) 298, 529, 828, 859
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 3 cut(s) 245, 506, 1025
FokI GGATG 1 cut(s) 809
Fsp4HI GCNGC 3 cut(s) 245, 506, 1025
FspBI CTAG 3 cut(s) 231, 516, 705
GlaI GCGC 1 cut(s) 458
GluI GCNGC 3 cut(s) 245, 506, 1025
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 4 cut(s) 72, 182, 703, 732
HhaI GCGC 1 cut(s) 459
Hin1II CATG 4 cut(s) 302, 533, 832, 863
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 3 cut(s) 113, 398, 607
HphI GGTGA 3 cut(s) 499, 515, 791
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 4 cut(s) 314, 425, 790, 922
Hpy188III TCNNGA 5 cut(s) 299, 343, 395, 550, 986
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 4 cut(s) 634, 678, 692, 944
HpyCH4V TGCA 2 cut(s) 405, 604
HpyF3I CTNAG 4 cut(s) 311, 494, 789, 921
Hsp92II CATG 4 cut(s) 302, 533, 832, 863
HspAI GCGC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 5 cut(s) 172, 391, 425, 445, 913
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 3 cut(s) 256, 517, 1036
LweI GCATC 1 cut(s) 334
MaeI CTAG 3 cut(s) 231, 516, 705
MaeIII GTNAC 2 cut(s) 779, 835
MalI GATC 5 cut(s) 174, 393, 427, 447, 915
MboI GATC 5 cut(s) 172, 391, 425, 445, 913
MfeI CAATTG 1 cut(s) 626
MflI RGATCY 3 cut(s) 172, 391, 913
MluCI AATT 5 cut(s) 102, 223, 626, 669, 966
MlyI GAGTC 2 cut(s) 107, 392
MmeI TCCRAC 1 cut(s) 394
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 3 cut(s) 60, 332, 756
MslI CAYNNNNRTG 3 cut(s) 366, 528, 833
MspR9I CCNGG 1 cut(s) 179
MunI CAATTG 1 cut(s) 626
MvaI CCWGG 1 cut(s) 179
MvnI CGCG 1 cut(s) 459
NcoI CCATGG 1 cut(s) 529
NdeII GATC 5 cut(s) 172, 391, 425, 445, 913
NlaIII CATG 4 cut(s) 302, 533, 832, 863
NlaIV GGNNCC 2 cut(s) 33, 174
NmuCI GTSAC 2 cut(s) 779, 835
NspI RCATGY 1 cut(s) 832
NspV TTCGAA 1 cut(s) 383
PagI TCATGA 1 cut(s) 298
PceI AGGCCT 1 cut(s) 182
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 607
PflMI CCANNNNNTGG 1 cut(s) 535
PkrI GCNGC 3 cut(s) 246, 507, 1026
PleI GAGTC 2 cut(s) 107, 392
PpsI GAGTC 2 cut(s) 107, 392
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 2 cut(s) 33, 174
PspPI GGNCC 3 cut(s) 71, 701, 856
PsrI GAACNNNNNNTAC 2 cut(s) 966, 998
PsuI RGATCY 3 cut(s) 172, 391, 913
RsaI GTAC 2 cut(s) 633, 975
RsaNI GTAC 2 cut(s) 632, 974
RseI CAYNNNNRTG 3 cut(s) 366, 528, 833
SaqAI TTAA 3 cut(s) 60, 332, 756
SatI GCNGC 3 cut(s) 245, 506, 1025
Sau3AI GATC 5 cut(s) 172, 391, 425, 445, 913
Sau96I GGNCC 3 cut(s) 71, 701, 856
ScaI AGTACT 1 cut(s) 975
SchI GAGTC 2 cut(s) 107, 392
ScrFI CCNGG 1 cut(s) 179
SfaNI GCATC 1 cut(s) 334
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 1 cut(s) 856
SmiMI CAYNNNNRTG 3 cut(s) 366, 528, 833
SmlI CTYRAG 2 cut(s) 548, 659
SmoI CTYRAG 2 cut(s) 548, 659
Sse9I AATT 5 cut(s) 102, 223, 626, 669, 966
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 1 cut(s) 688
SspI AATATT 1 cut(s) 773
SspMI CTAG 3 cut(s) 231, 516, 705
StuI AGGCCT 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 177
StyI CCWWGG 4 cut(s) 10, 67, 529, 733
TaqI TCGA 1 cut(s) 383
TasI AATT 5 cut(s) 102, 223, 626, 669, 966
TatI WGTACW 1 cut(s) 973
TfiI GAWTC 1 cut(s) 607
Tru1I TTAA 3 cut(s) 60, 332, 756
Tru9I TTAA 3 cut(s) 60, 332, 756
TscAI CASTG 3 cut(s) 54, 840, 1015
TseFI GTSAC 2 cut(s) 779, 835
TseI GCWGC 3 cut(s) 244, 505, 1024
Tsp45I GTSAC 2 cut(s) 779, 835
TspDTI ATGAA 8 cut(s) 17, 114, 315, 578, 858, 912, 963, 975
TspRI CASTG 3 cut(s) 54, 840, 1015
Van91I CCANNNNNTGG 1 cut(s) 535
VpaK11BI GGWCC 1 cut(s) 856
XceI RCATGY 1 cut(s) 832
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 3 cut(s) 231, 516, 705
ZrmI AGTACT 1 cut(s) 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.