MD16G1199800.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011743
Physical Location & Seq
Forward (+)
18064519 .. 18066128
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1199800.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGAATCTTGATCAAGACCCAGTAAGCTTTTCGTCTTCGAGTGCGGCCCCTGATCATCCTCTGCCATTGGAGAGGCTCTATGACCAAGGTCCTCCGGCATTTCTCACCAAAACGTATGACATCGTCGATGACCCATCAACCAACCACATTGTTTCATGGAGCAGAGACAACAACAGCTTTGTTGTTTTGGATCCCAATAGCTTTGCCATGAGTCTTCTCCCCAGATATTTCAAGCACAACAATTTCTCCAGCTTTGTCAGGCAGCTCAACACCTATGGATTTAGGAAGGTTGATACAGACAGATGGGAGTTTGCTAATGAAGCGTTTCTCAGAGGGCAAAAGCATCTTTTAAAGAATATCAGGAGGAGGAAGGCATCCCATCTTCCTCAGGCTTCACAGAAAACTTTGGACTCTTGTGTAGAAGTTGGGAGGTTTGGATTGGACATAGAGATCGACCAGTTGAGGCGCGATAAACAAGTACTAATGGGGGAACTAGTGAAGCTTCGACAGCAACAGCAAGCTAATAGAGTTTACCTCCAAGGAATGGAAGATAGACTGAAGAGGACAGAGTTGAAACAACAACATATGTTGAGTTTCTTGGCAAGGGCAATGCAGAATCCTAATTTTGTACAACAATTGTCTCAGCAGAAGGACTTAAGGAAGGAACTGGAGGAAGCAGTTACCAAGAAGAGAAGGCGGACTATTGAGAAAGGGCCTAGTAATGTTGAGGTGGATGAATTTGGCCAAGTTGGAGTAGAGACTTTTGTTAAAGTTGAACCACAAGAATATGGTGACATTTCTGATCAGTTTGAAGTTTCGGAGTTGGACCAATTTGCTATAGACATGCCGGGGGTAAGCGGAAGCCAAAATGTTCATGGTGACCAAGAATGTAAAGAGAGAGATGAAGAACATGAAGGTAGAGGCAAAGACCTGGATAAGGGTTTTTGGCAAGACTTGTTCAATGAGAATATTGATGAAGAAATTGGTTTGCTTGGTGTTCAAGAAGAGGATGAGGAAGATGTCAATGTGTTAATTGAGGAGCTTGGTTTTTTAGCTCCCAGTCCCAAGTAA

Protein Analysis

357

Amino Acids

41.09

Weight (kDa)

4.98

Isoelectric Point (pI)

58.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 34 - 123 3.3e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011743)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22830
fragaria_vesca FvH4_4g01180 FvH4_4g01180
malus_domestica MD13G1199700.v1.1 MD16G1199800.v1.1
prunus_persica Prupe.1G021200_v2.0.a1
pyrus_communis pycom13g17330 pycom16g16830
rosa_chinensis RchiOBHm_Chr4g0387751
rosa_laevigata RLG00000010097
rosa_multiflora Rmu_co8508987.1_g000001 Rmu_sc0008916.1_g000004
rosa_roxburghii Rroxscaffold_5G00334390
rosa_rugosa Rorug03G0313000
rosa_samantha Rh4AG018200 Rh4BG013100 Rh4CG019400 Rh4DG014000
rosa_wichuraiana Rw4G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 544
AccII CGCG 1 cut(s) 468
AciI CCGC 3 cut(s) 44, 697, 858
AclWI GGATC 2 cut(s) 185, 198
AcoI YGGCCR 1 cut(s) 742
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 1 cut(s) 578
AfaI GTAC 2 cut(s) 480, 630
AfiI CCNNNNNNNGG 2 cut(s) 544, 937
AflII CTTAAG 1 cut(s) 655
AgsI TTSAA 6 cut(s) 232, 574, 776, 812, 961, 1001
AhlI ACTAGT 1 cut(s) 493
AjnI CCWGG 1 cut(s) 930
AluBI AGCT 9 cut(s) 27, 177, 201, 252, 265, 502, 521, 1042, 1055
AluI AGCT 9 cut(s) 27, 177, 201, 252, 265, 502, 521, 1042, 1055
Alw26I GTCTC 3 cut(s) 159, 645, 752
AlwI GGATC 2 cut(s) 185, 198
AoxI GGCC 3 cut(s) 45, 713, 742
ApeKI GCWGC 1 cut(s) 262
ApoI RAATTY 1 cut(s) 737
Asp700I GAANNNNTTC 1 cut(s) 324
AspLEI GCGC 1 cut(s) 468
AspS9I GGNCC 4 cut(s) 46, 89, 713, 826
AsuC2I CCSGG 1 cut(s) 849
AsuHPI GGTGA 3 cut(s) 97, 803, 890
AvaII GGWCC 2 cut(s) 89, 826
AxyI CCTNAGG 1 cut(s) 387
BalI TGGCCA 1 cut(s) 744
BamHI GGATCC 1 cut(s) 190
BbsI GAAGAC 2 cut(s) 27, 206
BbvI GCAGC 1 cut(s) 274
BccI CCATC 3 cut(s) 142, 297, 387
BciT130I CCWGG 1 cut(s) 932
BclI TGATCA 3 cut(s) 10, 52, 802
BcnI CCSGG 1 cut(s) 849
BcoDI GTCTC 3 cut(s) 159, 645, 752
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 2 cut(s) 494, 717
BfmI CTRYAG 1 cut(s) 837
BfrI CTTAAG 1 cut(s) 655
BisI GCNGC 2 cut(s) 45, 263
BlsI GCNGC 2 cut(s) 46, 264
BmcAI AGTACT 1 cut(s) 480
Bme1390I CCNGG 2 cut(s) 849, 932
Bme18I GGWCC 2 cut(s) 89, 826
BmgT120I GGNCC 4 cut(s) 46, 89, 713, 826
BmiI GGNNCC 2 cut(s) 48, 192
BmrFI CCNGG 2 cut(s) 849, 932
BmrI ACTGGG 2 cut(s) 14, 1053
BmsI GCATC 2 cut(s) 352, 383
BmuI ACTGGG 2 cut(s) 14, 1053
BoxI GACNNNNGTC 1 cut(s) 87
BpiI GAAGAC 2 cut(s) 27, 206
BplI GAGNNNNNCTC 2 cut(s) 519, 551
BpmI CTGGAG 2 cut(s) 232, 689
BpuMI CCSGG 1 cut(s) 849
BsaJI CCNNGG 3 cut(s) 85, 538, 848
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 2 cut(s) 544, 937
Bse1I ACTGG 4 cut(s) 20, 457, 672, 1059
Bse21I CCTNAGG 1 cut(s) 387
Bse3DI GCAATG 1 cut(s) 615
BseBI CCWGG 1 cut(s) 932
BseDI CCNNGG 3 cut(s) 85, 538, 848
BseGI GGATG 4 cut(s) 55, 374, 739, 1015
BseLI CCNNNNNNNGG 2 cut(s) 544, 937
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 3 cut(s) 343, 401, 656
BseNI ACTGG 4 cut(s) 20, 457, 672, 1059
BseRI GAGGAG 2 cut(s) 379, 1052
BseXI GCAGC 1 cut(s) 274
Bsh1236I CGCG 1 cut(s) 468
BshFI GGCC 3 cut(s) 47, 715, 744
BsiSI CCGG 2 cut(s) 95, 848
BslFI GGGAC 1 cut(s) 1047
BslI CCNNNNNNNGG 2 cut(s) 544, 937
BsmAI GTCTC 3 cut(s) 159, 645, 752
BsmFI GGGAC 1 cut(s) 1047
BsnI GGCC 3 cut(s) 47, 715, 744
Bsp1407I TGTACA 1 cut(s) 628
Bsp143I GATC 5 cut(s) 10, 52, 190, 450, 802
BspACI CCGC 3 cut(s) 44, 697, 858
BspANI GGCC 3 cut(s) 47, 715, 744
BspCNI CTCAG 3 cut(s) 342, 400, 655
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 2 cut(s) 48, 192
BspPI GGATC 2 cut(s) 185, 198
BspTI CTTAAG 1 cut(s) 655
BsrDI GCAATG 1 cut(s) 615
BsrGI TGTACA 1 cut(s) 628
BsrI ACTGG 4 cut(s) 20, 457, 672, 1059
BssECI CCNNGG 3 cut(s) 85, 538, 848
BssMI GATC 5 cut(s) 10, 52, 190, 450, 802
BssT1I CCWWGG 2 cut(s) 85, 538
Bst2UI CCWGG 1 cut(s) 932
Bst6I CTCTTC 3 cut(s) 554, 683, 999
BstAFI CTTAAG 1 cut(s) 655
BstAUI TGTACA 1 cut(s) 628
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 3 cut(s) 329, 387, 642
BstEII GGTNACC 1 cut(s) 878
BstENI CCTNNNNNAGG 1 cut(s) 935
BstF5I GGATG 4 cut(s) 55, 374, 739, 1015
BstFNI CGCG 1 cut(s) 468
BstHHI GCGC 1 cut(s) 468
BstKTI GATC 5 cut(s) 13, 55, 193, 453, 805
BstMAI GTCTC 3 cut(s) 159, 645, 752
BstMBI GATC 5 cut(s) 10, 52, 190, 450, 802
BstMWI GCNNNNNNNGC 2 cut(s) 320, 508
BstNI CCWGG 1 cut(s) 932
BstNSI RCATGY 1 cut(s) 847
BstPAI GACNNNNGTC 1 cut(s) 87
BstPI GGTNACC 1 cut(s) 878
BstSCI CCNGG 2 cut(s) 847, 930
BstSFI CTRYAG 1 cut(s) 837
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 2 cut(s) 27, 206
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
Bsu36I CCTNAGG 1 cut(s) 387
BsuRI GGCC 3 cut(s) 47, 715, 744
BtsCI GGATG 4 cut(s) 55, 374, 739, 1015
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 1 cut(s) 468
Cfr13I GGNCC 4 cut(s) 46, 89, 713, 826
Csp6I GTAC 2 cut(s) 479, 629
CviAII CATG 5 cut(s) 156, 208, 844, 875, 911
CviQI GTAC 2 cut(s) 479, 629
DdeI CTNAG 3 cut(s) 329, 387, 642
DpnI GATC 5 cut(s) 12, 54, 192, 452, 804
DpnII GATC 5 cut(s) 10, 52, 190, 450, 802
DraI TTTAAA 1 cut(s) 351
EaeI YGGCCR 1 cut(s) 742
Eam1104I CTCTTC 3 cut(s) 554, 683, 999
EarI CTCTTC 3 cut(s) 554, 683, 999
EciI GGCGGA 1 cut(s) 712
Eco130I CCWWGG 2 cut(s) 85, 538
Eco47I GGWCC 2 cut(s) 89, 826
Eco57I CTGAAG 1 cut(s) 578
Eco81I CCTNAGG 1 cut(s) 387
Eco91I GGTNACC 1 cut(s) 878
EcoNI CCTNNNNNAGG 1 cut(s) 935
EcoO109I RGGNCCY 2 cut(s) 89, 713
EcoO65I GGTNACC 1 cut(s) 878
EcoRII CCWGG 1 cut(s) 930
EcoT14I CCWWGG 2 cut(s) 85, 538
ErhI CCWWGG 2 cut(s) 85, 538
FaeI CATG 5 cut(s) 159, 211, 847, 878, 914
FaqI GGGAC 1 cut(s) 1047
FatI CATG 5 cut(s) 155, 207, 843, 874, 910
FauNDI CATATG 1 cut(s) 585
FbaI TGATCA 3 cut(s) 10, 52, 802
Fnu4HI GCNGC 2 cut(s) 45, 263
FokI GGATG 4 cut(s) 42, 361, 746, 1022
Fsp4HI GCNGC 2 cut(s) 45, 263
FspBI CTAG 2 cut(s) 494, 717
GlaI GCGC 1 cut(s) 467
GluI GCNGC 2 cut(s) 45, 263
GsuI CTGGAG 2 cut(s) 232, 689
HaeIII GGCC 3 cut(s) 47, 715, 744
HapII CCGG 2 cut(s) 95, 848
HhaI GCGC 1 cut(s) 468
Hin1II CATG 5 cut(s) 159, 211, 847, 878, 914
Hin6I GCGC 1 cut(s) 466
HinP1I GCGC 1 cut(s) 466
HindIII AAGCTT 2 cut(s) 25, 500
HinfI GANTC 4 cut(s) 4, 211, 410, 616
HpaII CCGG 2 cut(s) 95, 848
HphI GGTGA 3 cut(s) 97, 803, 890
Hpy166II GTNNAC 1 cut(s) 532
Hpy188I TCNGA 3 cut(s) 332, 802, 820
Hpy188III TCNNGA 4 cut(s) 8, 14, 361, 1001
Hpy8I GTNNAC 1 cut(s) 532
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 6 cut(s) 280, 364, 643, 655, 687, 908
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 1 cut(s) 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 508
HpyF3I CTNAG 3 cut(s) 329, 387, 642
HpySE526I ACGT 1 cut(s) 113
Hsp92II CATG 5 cut(s) 159, 211, 847, 878, 914
HspAI GCGC 1 cut(s) 466
Ksp22I TGATCA 3 cut(s) 10, 52, 802
Kzo9I GATC 5 cut(s) 10, 52, 190, 450, 802
LmnI GCTCC 3 cut(s) 159, 1039, 1060
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 2 cut(s) 352, 383
MaeI CTAG 2 cut(s) 494, 717
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 3 cut(s) 679, 791, 878
MalI GATC 5 cut(s) 12, 54, 192, 452, 804
MboI GATC 5 cut(s) 10, 52, 190, 450, 802
MfeI CAATTG 1 cut(s) 635
MflI RGATCY 1 cut(s) 190
MlsI TGGCCA 1 cut(s) 744
MluCI AATT 7 cut(s) 241, 622, 635, 737, 830, 981, 1032
MluNI TGGCCA 1 cut(s) 744
MlyI GAGTC 2 cut(s) 220, 404
MmeI TCCRAC 2 cut(s) 730, 804
Mox20I TGGCCA 1 cut(s) 744
MroXI GAANNNNTTC 1 cut(s) 324
MscI TGGCCA 1 cut(s) 744
MseI TTAA 4 cut(s) 350, 656, 768, 1031
Msp20I TGGCCA 1 cut(s) 744
MspCI CTTAAG 1 cut(s) 655
MspI CCGG 2 cut(s) 95, 848
MspR9I CCNGG 2 cut(s) 849, 932
MunI CAATTG 1 cut(s) 635
MvaI CCWGG 1 cut(s) 932
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 320, 508
NciI CCSGG 1 cut(s) 849
NdeI CATATG 1 cut(s) 585
NdeII GATC 5 cut(s) 10, 52, 190, 450, 802
NlaIII CATG 5 cut(s) 159, 211, 847, 878, 914
NlaIV GGNNCC 2 cut(s) 48, 192
NmuCI GTSAC 2 cut(s) 791, 878
NspI RCATGY 1 cut(s) 847
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 2 cut(s) 4, 616
PflFI GACNNNGTC 1 cut(s) 122
PflMI CCANNNNNTGG 1 cut(s) 544
PkrI GCNGC 2 cut(s) 46, 264
PleI GAGTC 2 cut(s) 219, 404
PpsI GAGTC 2 cut(s) 219, 404
PpuMI RGGWCCY 1 cut(s) 89
PshAI GACNNNNGTC 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 930
PspEI GGTNACC 1 cut(s) 878
PspGI CCWGG 1 cut(s) 930
PspN4I GGNNCC 2 cut(s) 48, 192
PspPI GGNCC 4 cut(s) 46, 89, 713, 826
PspPPI RGGWCCY 1 cut(s) 89
PsuI RGATCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 122
RsaI GTAC 2 cut(s) 480, 630
RsaNI GTAC 2 cut(s) 479, 629
SaqAI TTAA 4 cut(s) 350, 656, 768, 1031
SatI GCNGC 2 cut(s) 45, 263
Sau3AI GATC 5 cut(s) 10, 52, 190, 450, 802
Sau96I GGNCC 4 cut(s) 46, 89, 713, 826
ScaI AGTACT 1 cut(s) 480
SchI GAGTC 2 cut(s) 220, 404
ScrFI CCNGG 2 cut(s) 849, 932
SfaNI GCATC 2 cut(s) 352, 383
SfcI CTRYAG 1 cut(s) 837
SinI GGWCC 2 cut(s) 89, 826
SmlI CTYRAG 1 cut(s) 655
SmoI CTYRAG 1 cut(s) 655
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 7 cut(s) 241, 622, 635, 737, 830, 981, 1032
SsiI CCGC 3 cut(s) 44, 697, 858
SspI AATATT 1 cut(s) 970
SspMI CTAG 2 cut(s) 494, 717
StyD4I CCNGG 2 cut(s) 847, 930
StyI CCWWGG 2 cut(s) 85, 538
TaiI ACGT 1 cut(s) 116
TaqI TCGA 4 cut(s) 38, 126, 453, 505
TasI AATT 7 cut(s) 241, 622, 635, 737, 830, 981, 1032
TatI WGTACW 2 cut(s) 478, 628
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 2 cut(s) 4, 616
Tru1I TTAA 4 cut(s) 350, 656, 768, 1031
Tru9I TTAA 4 cut(s) 350, 656, 768, 1031
TseFI GTSAC 2 cut(s) 791, 878
TseI GCWGC 1 cut(s) 262
Tsp45I GTSAC 2 cut(s) 791, 878
TspDTI ATGAA 8 cut(s) 17, 144, 333, 750, 863, 918, 927, 990
Tth111I GACNNNGTC 1 cut(s) 122
Van91I CCANNNNNTGG 1 cut(s) 544
Vha464I CTTAAG 1 cut(s) 655
VpaK11BI GGWCC 2 cut(s) 89, 826
XagI CCTNNNNNAGG 1 cut(s) 935
XapI RAATTY 1 cut(s) 737
XceI RCATGY 1 cut(s) 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 2 cut(s) 494, 717
ZrmI AGTACT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.