Rh4AG018200

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011743
Physical Location & Seq
Forward (+)
3541321 .. 3543241
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG018200.1

Sequence Viewer

Length: 1056 bp
ATGAACCCCCAAGGTCAAGTAAAGGGGGCACCTTTACCTGTGCCACTGGAGAGACTTAATGACCAAGGCCCTCCACCATTTCTCAACAAAACCTATGAAATTGTGGAAGACTCAACAACCAACCACATAGTGTCTTGGAGCAAAGCAAACAACAGCTTTGTTGTATGGGATCCCCAGGCCTTTGCCATCAGTCTTCTCCCCAAGTACTTCAAGCACGGCAATTTCTCTAGCTTTGTCAGGCAGCTCAACACCTATGGATTTAGAAAGGTTGACACAGATAAATGGGAGTTTGCTCATGAAGTGTTTCTCAGAGGGCAAAAGCATCTTTTAAAGAATATCAGGAGGAGGAGGACATCTCATCATAATGTTCATTATGGTTCGAAGCAAGATCTGGACTCTTGCATTGAGGTTGGAAGTTTCGGATCATTAGATGGAGAGATTGATCAGTTGAGGCGCGATAAGGAGGTTTTGATGGGGGAACTGGTGAAGCTTAGACAACAGCAGCAGACTGCTAGACTTCACCTCCATGGAATGGAAGATAGGCTCAAGAGGACAGAGATGAAACAACAACAGATGATGAATTTCTTGGCAAGAGCAATGCAGAATCCCAACTTTTTACAACAATTGGTACAAAAGAAGGAAAGAAAGAAACAACTTGAGGAAGCAATTACGAACAAGAGAAGGCGGACTATTGAAGGGCCTAGTAGTATTGAGGTGGGTGCATTAGGCCAGGGTGGAGGAGAAGCTGTTGTTAAAGTTGAACCCGAATATTATGGTGACATATCTGAGCTGGATGTTTCAGGGTTAGACAACTTTGCTATGGACATGCCAGTGACAGATGAAAACGAAAAGGTCCACCATGATGGGGAAGAGTGTATGGAGAAAGAAGAGGAATATGGAAGGAGAGGCAAAGATCTGTCTGAGGTATTTTGGAAAGAGTTTTTGAATGAAGGCGTTGATGAAGAAATTGAAGTACTCGGTGTTCAAGAAGAAGATGAGGAAGATGTCAGTGTGTTGATTGAGCAGCTTGGTTGCTTGGTGTCCAAGCCCAAGTAA

Protein Analysis

351

Amino Acids

40.41

Weight (kDa)

5.44

Isoelectric Point (pI)

60.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 27 - 116 6.2e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011743)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22830
fragaria_vesca FvH4_4g01180 FvH4_4g01180
malus_domestica MD13G1199700.v1.1 MD16G1199800.v1.1
prunus_persica Prupe.1G021200_v2.0.a1
pyrus_communis pycom13g17330 pycom16g16830
rosa_chinensis RchiOBHm_Chr4g0387751
rosa_laevigata RLG00000010097
rosa_multiflora Rmu_co8508987.1_g000001 Rmu_sc0008916.1_g000004
rosa_roxburghii Rroxscaffold_5G00334390
rosa_rugosa Rorug03G0313000
rosa_samantha Rh4AG018200 Rh4BG013100 Rh4CG019400 Rh4DG014000
rosa_wichuraiana Rw4G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 28
AccB7I CCANNNNNTGG 1 cut(s) 532
AccII CGCG 1 cut(s) 456
AciI CCGC 1 cut(s) 685
AclWI GGATC 3 cut(s) 164, 177, 430
AcsI RAATTY 1 cut(s) 580
AdeI CACNNNGTG 1 cut(s) 130
AfaI GTAC 3 cut(s) 206, 630, 975
AfiI CCNNNNNNNGG 2 cut(s) 532, 865
AgsI TTSAA 6 cut(s) 211, 695, 761, 946, 971, 986
AjnI CCWGG 2 cut(s) 174, 729
AluBI AGCT 7 cut(s) 156, 231, 244, 490, 746, 790, 1027
AluI AGCT 7 cut(s) 156, 231, 244, 490, 746, 790, 1027
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 3 cut(s) 164, 177, 430
AoxI GGCC 4 cut(s) 67, 177, 698, 727
ApeKI GCWGC 3 cut(s) 241, 502, 1024
ApoI RAATTY 1 cut(s) 580
Asp700I GAANNNNTTC 1 cut(s) 303
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 3 cut(s) 68, 698, 853
AsuHPI GGTGA 3 cut(s) 496, 512, 788
AsuII TTCGAA 1 cut(s) 380
AvaII GGWCC 1 cut(s) 853
BaeGI GKGCMC 1 cut(s) 31
BamHI GGATCC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 28
BbsI GAAGAC 2 cut(s) 114, 185
BbvI GCAGC 3 cut(s) 253, 514, 1036
BccI CCATC 4 cut(s) 194, 425, 466, 857
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 176, 731
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 3 cut(s) 228, 513, 702
BglII AGATCT 2 cut(s) 388, 913
BisI GCNGC 3 cut(s) 242, 503, 1025
BlsI GCNGC 3 cut(s) 243, 504, 1026
BmcAI AGTACT 2 cut(s) 206, 975
Bme1390I CCNGG 2 cut(s) 176, 731
Bme18I GGWCC 1 cut(s) 853
BmgT120I GGNCC 3 cut(s) 68, 698, 853
BmiI GGNNCC 2 cut(s) 30, 171
BmrFI CCNGG 2 cut(s) 176, 731
BmsI GCATC 1 cut(s) 331
BpiI GAAGAC 2 cut(s) 114, 185
BplI GAGNNNNNCTC 2 cut(s) 340, 372
BpmI CTGGAG 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 380
BpuEI CTTGAG 2 cut(s) 530, 677
BsaJI CCNNGG 5 cut(s) 10, 64, 174, 526, 730
BsaXI ACNNNNNCTCC 4 cut(s) 507, 537, 732, 762
Bsc4I CCNNNNNNNGG 2 cut(s) 532, 865
Bse1I ACTGG 3 cut(s) 51, 486, 830
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 2 cut(s) 176, 731
BseDI CCNNGG 5 cut(s) 10, 64, 174, 526, 730
BseGI GGATG 1 cut(s) 799
BseLI CCNNNNNNNGG 2 cut(s) 532, 865
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 3 cut(s) 322, 777, 912
BseNI ACTGG 3 cut(s) 51, 486, 830
BseRI GAGGAG 3 cut(s) 358, 361, 753
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 3 cut(s) 253, 514, 1036
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 4 cut(s) 69, 179, 700, 729
BshNI GGYRCC 1 cut(s) 28
BslI CCNNNNNNNGG 2 cut(s) 532, 865
BsmAI GTCTC 1 cut(s) 46
BsnI GGCC 4 cut(s) 69, 179, 700, 729
Bsp119I TTCGAA 1 cut(s) 380
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 5 cut(s) 169, 388, 422, 442, 913
Bsp19I CCATGG 1 cut(s) 526
BspACI CCGC 1 cut(s) 685
BspANI GGCC 4 cut(s) 69, 179, 700, 729
BspCNI CTCAG 3 cut(s) 321, 778, 913
BspFNI CGCG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 30, 171
BspPI GGATC 3 cut(s) 164, 177, 430
BspT104I TTCGAA 1 cut(s) 380
BspT107I GGYRCC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 3 cut(s) 51, 486, 830
BssECI CCNNGG 5 cut(s) 10, 64, 174, 526, 730
BssMI GATC 5 cut(s) 169, 388, 422, 442, 913
BssT1I CCWWGG 3 cut(s) 10, 64, 526
Bst2UI CCWGG 2 cut(s) 176, 731
Bst6I CTCTTC 2 cut(s) 864, 882
BstBI TTCGAA 1 cut(s) 380
BstDEI CTNAG 4 cut(s) 308, 491, 786, 921
BstDSI CCRYGG 1 cut(s) 526
BstF5I GGATG 1 cut(s) 799
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 5 cut(s) 172, 391, 425, 445, 916
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 5 cut(s) 169, 388, 422, 442, 913
BstNI CCWGG 2 cut(s) 176, 731
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 2 cut(s) 174, 729
BstSLI GKGCMC 1 cut(s) 31
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 3 cut(s) 253, 514, 1036
BstV2I GAAGAC 2 cut(s) 114, 185
BstX2I RGATCY 3 cut(s) 169, 388, 913
BstXI CCANNNNNNTGG 1 cut(s) 863
BstYI RGATCY 3 cut(s) 169, 388, 913
BsuRI GGCC 4 cut(s) 69, 179, 700, 729
BtgI CCRYGG 1 cut(s) 526
BtsCI GGATG 1 cut(s) 799
BtsIMutI CAGTG 3 cut(s) 44, 837, 1015
CciI TCATGA 1 cut(s) 295
CfoI GCGC 1 cut(s) 456
Cfr13I GGNCC 3 cut(s) 68, 698, 853
Csp6I GTAC 3 cut(s) 205, 629, 974
CviAII CATG 4 cut(s) 296, 527, 826, 860
CviQI GTAC 3 cut(s) 205, 629, 974
DdeI CTNAG 4 cut(s) 308, 491, 786, 921
DpnI GATC 5 cut(s) 171, 390, 424, 444, 915
DpnII GATC 5 cut(s) 169, 388, 422, 442, 913
DraI TTTAAA 1 cut(s) 330
DraIII CACNNNGTG 1 cut(s) 130
Eam1104I CTCTTC 2 cut(s) 864, 882
EarI CTCTTC 2 cut(s) 864, 882
EciI GGCGGA 1 cut(s) 700
Eco130I CCWWGG 3 cut(s) 10, 64, 526
Eco147I AGGCCT 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 853
EcoO109I RGGNCCY 2 cut(s) 68, 698
EcoRII CCWGG 2 cut(s) 174, 729
EcoT14I CCWWGG 3 cut(s) 10, 64, 526
ErhI CCWWGG 3 cut(s) 10, 64, 526
FaeI CATG 4 cut(s) 299, 530, 829, 863
FatI CATG 4 cut(s) 295, 526, 825, 859
FbaI TGATCA 1 cut(s) 442
Fnu4HI GCNGC 3 cut(s) 242, 503, 1025
FokI GGATG 1 cut(s) 806
Fsp4HI GCNGC 3 cut(s) 242, 503, 1025
FspBI CTAG 3 cut(s) 228, 513, 702
GlaI GCGC 1 cut(s) 455
GluI GCNGC 3 cut(s) 242, 503, 1025
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 4 cut(s) 69, 179, 700, 729
HhaI GCGC 1 cut(s) 456
Hin1II CATG 4 cut(s) 299, 530, 829, 863
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 3 cut(s) 110, 395, 604
HphI GGTGA 3 cut(s) 496, 512, 788
Hpy166II GTNNAC 2 cut(s) 271, 856
Hpy188I TCNGA 4 cut(s) 311, 422, 787, 922
Hpy188III TCNNGA 5 cut(s) 296, 340, 392, 547, 986
Hpy8I GTNNAC 2 cut(s) 271, 856
HpyAV CCTTC 5 cut(s) 631, 675, 689, 894, 944
HpyCH4V TGCA 3 cut(s) 402, 601, 722
HpyF3I CTNAG 4 cut(s) 308, 491, 786, 921
Hsp92II CATG 4 cut(s) 299, 530, 829, 863
HspAI GCGC 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 5 cut(s) 169, 388, 422, 442, 913
LmnI GCTCC 1 cut(s) 138
Lsp1109I GCAGC 3 cut(s) 253, 514, 1036
LweI GCATC 1 cut(s) 331
MaeI CTAG 3 cut(s) 228, 513, 702
MaeIII GTNAC 2 cut(s) 776, 832
MalI GATC 5 cut(s) 171, 390, 424, 444, 915
MboI GATC 5 cut(s) 169, 388, 422, 442, 913
MboII GAAGA 9 cut(s) 119, 185, 548, 881, 899, 974, 1001, 1004, 1013
MfeI CAATTG 1 cut(s) 623
MflI RGATCY 3 cut(s) 169, 388, 913
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 6 cut(s) 99, 220, 580, 623, 666, 966
MlyI GAGTC 2 cut(s) 104, 389
MmeI TCCRAC 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 3 cut(s) 57, 329, 753
MslI CAYNNNNRTG 4 cut(s) 363, 525, 830, 861
MspR9I CCNGG 2 cut(s) 176, 731
MunI CAATTG 1 cut(s) 623
MvaI CCWGG 2 cut(s) 176, 731
MvnI CGCG 1 cut(s) 456
NcoI CCATGG 1 cut(s) 526
NdeII GATC 5 cut(s) 169, 388, 422, 442, 913
NlaIII CATG 4 cut(s) 299, 530, 829, 863
NlaIV GGNNCC 2 cut(s) 30, 171
NmuCI GTSAC 2 cut(s) 776, 832
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 380
PagI TCATGA 1 cut(s) 295
PceI AGGCCT 1 cut(s) 179
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 604
PflMI CCANNNNNTGG 1 cut(s) 532
PkrI GCNGC 3 cut(s) 243, 504, 1026
PleI GAGTC 2 cut(s) 104, 389
PpsI GAGTC 2 cut(s) 104, 389
Psp6I CCWGG 2 cut(s) 174, 729
PspGI CCWGG 2 cut(s) 174, 729
PspN4I GGNNCC 2 cut(s) 30, 171
PspPI GGNCC 3 cut(s) 68, 698, 853
PsrI GAACNNNNNNTAC 2 cut(s) 966, 998
PsuI RGATCY 3 cut(s) 169, 388, 913
RsaI GTAC 3 cut(s) 206, 630, 975
RsaNI GTAC 3 cut(s) 205, 629, 974
RseI CAYNNNNRTG 4 cut(s) 363, 525, 830, 861
SaqAI TTAA 3 cut(s) 57, 329, 753
SatI GCNGC 3 cut(s) 242, 503, 1025
Sau3AI GATC 5 cut(s) 169, 388, 422, 442, 913
Sau96I GGNCC 3 cut(s) 68, 698, 853
ScaI AGTACT 2 cut(s) 206, 975
SchI GAGTC 2 cut(s) 104, 389
ScrFI CCNGG 2 cut(s) 176, 731
SduI GDGCHC 1 cut(s) 31
SfaNI GCATC 1 cut(s) 331
SfuI TTCGAA 1 cut(s) 380
SinI GGWCC 1 cut(s) 853
SmiMI CAYNNNNRTG 4 cut(s) 363, 525, 830, 861
SmlI CTYRAG 2 cut(s) 545, 656
SmoI CTYRAG 2 cut(s) 545, 656
Sse9I AATT 6 cut(s) 99, 220, 580, 623, 666, 966
SseBI AGGCCT 1 cut(s) 179
SsiI CCGC 1 cut(s) 685
SspI AATATT 1 cut(s) 770
SspMI CTAG 3 cut(s) 228, 513, 702
StuI AGGCCT 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 174, 729
StyI CCWWGG 3 cut(s) 10, 64, 526
TaqI TCGA 1 cut(s) 380
TasI AATT 6 cut(s) 99, 220, 580, 623, 666, 966
TatI WGTACW 2 cut(s) 204, 973
TfiI GAWTC 1 cut(s) 604
Tru1I TTAA 3 cut(s) 57, 329, 753
Tru9I TTAA 3 cut(s) 57, 329, 753
TscAI CASTG 3 cut(s) 51, 837, 1015
TseFI GTSAC 2 cut(s) 776, 832
TseI GCWGC 3 cut(s) 241, 502, 1024
Tsp45I GTSAC 2 cut(s) 776, 832
TspDTI ATGAA 9 cut(s) 17, 111, 312, 359, 575, 593, 855, 963, 975
TspRI CASTG 3 cut(s) 51, 837, 1015
Van91I CCANNNNNTGG 1 cut(s) 532
VpaK11BI GGWCC 1 cut(s) 853
XapI RAATTY 1 cut(s) 580
XceI RCATGY 1 cut(s) 829
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 3 cut(s) 228, 513, 702
ZrmI AGTACT 2 cut(s) 206, 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.