pycom13g17330

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011743
Physical Location & Seq
Forward (+)
13110034 .. 13111694
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g17330.1

Sequence Viewer

Length: 1071 bp
ATGAATCTTGATCAAGACCCAGTAAGCTTTTCTTCTTCAAGTGCGGTCGCTGATCATCCTCTACCACTGGAGAAGCTCTATGACCAAGGCCCTCCTCCATTTCTCACCAAAACTTATGACATCGTCGACGACCCATCAACCAATCACATAGTTTCATGGAGTAGAGACAACAACAGCTTTGTTGTTTTGGATCCCAATAGCTTTGCCATGAGTCTTCTCCCCAGATACTTCAAGCATAACAACTTCTCCAGCTTTGTCAGGCAGCTCAACACCTATGGATTTAGGAAGGTTGATACAGATAGATGGGAGTTTGCTAATGAAGCGTTTCTCAGAGGGAAAAAGCATCTTTTAAAGAATATCAGGAGAAGGAAGGCATCCCATCTTCCTCAGTCTTCACAAAAAACTTTGGACTCTTGTGTTGAAGTTGGAAGGTTTGGATTGGACATAGAGATCGATCAGTTGAGGCGCGATAAACAGGTACTAATGGGGGAACTAGTGAAGCTTAGACAGCAACAGCAAGCTACTAGAGTCTACATCCAAGGAATGGAAGACAGACTGAAAAGGACAGAAATGAAACAGCAACATATGTTGAGTTTCTTGGCAAAGGCAATGCAGAATCCCAACTTTGTACAACAATTGTCGCAGCAGACCGACTTTAGGAAAGAACTTGAGGAAGCGATTACCAAGAAGAGAAGGCGGACTATTGAGCAAGGCCCTAGTAATGTTGAGGTGGATGAATTAGGCCAAGTTGGAGGAGAGACTTTTGTTAAAGTTGAACCGCAAGAATATGGTAATATTTCTGATCAGTTTGAAGTTTCGGAGTTGGACCAATTTGCTATGGATATGCCGGGTGTAAGCGGAACCCAAAACGTCCTTGGTGAGGAAGAATGTACAGAGAAAGAAGAGGAACGTGAGAGTAGAGGCAAAGACCTGGATAAGGGGTTTTGGCAAGAATTGTTCGATGAGAGTATTGATGAAGAAATTGATTTGCTTGGTGTTCAAGAAGAAGATGAGGAAGATGTCGATGTGTTAATTGAGGAGCTTGGTTTCTTGGCTCCCAGTCCCAAGTAA

Protein Analysis

357

Amino Acids

41.1

Weight (kDa)

4.78

Isoelectric Point (pI)

63.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 34 - 123 4.2e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011743)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22830
fragaria_vesca FvH4_4g01180 FvH4_4g01180
malus_domestica MD13G1199700.v1.1 MD16G1199800.v1.1
prunus_persica Prupe.1G021200_v2.0.a1
pyrus_communis pycom13g17330 pycom16g16830
rosa_chinensis RchiOBHm_Chr4g0387751
rosa_laevigata RLG00000010097
rosa_multiflora Rmu_co8508987.1_g000001 Rmu_sc0008916.1_g000004
rosa_roxburghii Rroxscaffold_5G00334390
rosa_rugosa Rorug03G0313000
rosa_samantha Rh4AG018200 Rh4BG013100 Rh4CG019400 Rh4DG014000
rosa_wichuraiana Rw4G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 544
AccI GTMKAC 2 cut(s) 126, 531
AccII CGCG 1 cut(s) 468
AciI CCGC 4 cut(s) 44, 697, 779, 858
AclWI GGATC 2 cut(s) 185, 198
AfaI GTAC 3 cut(s) 480, 630, 892
AfiI CCNNNNNNNGG 4 cut(s) 544, 657, 880, 937
AgsI TTSAA 6 cut(s) 39, 232, 422, 776, 812, 1001
AhlI ACTAGT 1 cut(s) 493
AjnI CCWGG 1 cut(s) 930
AluBI AGCT 9 cut(s) 27, 76, 177, 201, 252, 265, 502, 521, 1042
AluI AGCT 9 cut(s) 27, 76, 177, 201, 252, 265, 502, 521, 1042
Alw26I GTCTC 2 cut(s) 159, 752
AlwI GGATC 2 cut(s) 185, 198
AoxI GGCC 3 cut(s) 88, 712, 742
ApeKI GCWGC 2 cut(s) 262, 643
Asp700I GAANNNNTTC 1 cut(s) 324
AspLEI GCGC 1 cut(s) 468
AspS9I GGNCC 3 cut(s) 89, 713, 826
AsuC2I CCSGG 1 cut(s) 849
AsuHPI GGTGA 2 cut(s) 97, 890
AvaII GGWCC 1 cut(s) 826
BamHI GGATCC 1 cut(s) 190
BbsI GAAGAC 3 cut(s) 206, 384, 555
BbvI GCAGC 2 cut(s) 274, 655
BccI CCATC 3 cut(s) 142, 297, 387
BciT130I CCWGG 1 cut(s) 932
BclI TGATCA 3 cut(s) 10, 52, 802
BcnI CCSGG 1 cut(s) 849
BcoDI GTCTC 2 cut(s) 159, 752
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 3 cut(s) 494, 525, 717
BisI GCNGC 2 cut(s) 263, 644
BlsI GCNGC 2 cut(s) 264, 645
Bme1390I CCNGG 2 cut(s) 849, 932
Bme18I GGWCC 1 cut(s) 826
BmgT120I GGNCC 3 cut(s) 89, 713, 826
BmiI GGNNCC 3 cut(s) 192, 862, 1056
BmrFI CCNGG 2 cut(s) 849, 932
BmrI ACTGGG 2 cut(s) 14, 1053
BmsI GCATC 2 cut(s) 352, 383
BmuI ACTGGG 2 cut(s) 14, 1053
BpiI GAAGAC 3 cut(s) 206, 384, 555
BpmI CTGGAG 2 cut(s) 89, 232
BpuEI CTTGAG 1 cut(s) 689
BpuMI CCSGG 1 cut(s) 849
Bsa29I ATCGAT 1 cut(s) 453
BsaJI CCNNGG 3 cut(s) 85, 538, 874
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 4 cut(s) 544, 657, 880, 937
Bse1I ACTGG 3 cut(s) 20, 72, 1059
Bse3DI GCAATG 1 cut(s) 615
BseBI CCWGG 1 cut(s) 932
BseCI ATCGAT 1 cut(s) 453
BseDI CCNNGG 3 cut(s) 85, 538, 874
BseGI GGATG 4 cut(s) 55, 374, 534, 739
BseLI CCNNNNNNNGG 4 cut(s) 544, 657, 880, 937
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 2 cut(s) 343, 401
BseNI ACTGG 3 cut(s) 20, 72, 1059
BseRI GAGGAG 3 cut(s) 84, 768, 1052
BseXI GCAGC 2 cut(s) 274, 655
Bsh1236I CGCG 1 cut(s) 468
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 3 cut(s) 90, 714, 744
BshVI ATCGAT 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 48
BsiSI CCGG 1 cut(s) 848
BslFI GGGAC 1 cut(s) 1047
BslI CCNNNNNNNGG 4 cut(s) 544, 657, 880, 937
BsmAI GTCTC 2 cut(s) 159, 752
BsmFI GGGAC 1 cut(s) 1047
BsnI GGCC 3 cut(s) 90, 714, 744
Bsp1407I TGTACA 2 cut(s) 628, 890
Bsp143I GATC 6 cut(s) 10, 52, 190, 450, 454, 802
BspACI CCGC 4 cut(s) 44, 697, 779, 858
BspANI GGCC 3 cut(s) 90, 714, 744
BspCNI CTCAG 2 cut(s) 342, 400
BspDI ATCGAT 1 cut(s) 453
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 3 cut(s) 192, 862, 1056
BspPI GGATC 2 cut(s) 185, 198
BsrDI GCAATG 1 cut(s) 615
BsrGI TGTACA 2 cut(s) 628, 890
BsrI ACTGG 3 cut(s) 20, 72, 1059
BssECI CCNNGG 3 cut(s) 85, 538, 874
BssMI GATC 6 cut(s) 10, 52, 190, 450, 454, 802
BssT1I CCWWGG 3 cut(s) 85, 538, 874
Bst2UI CCWGG 1 cut(s) 932
Bst6I CTCTTC 2 cut(s) 683, 897
BstAUI TGTACA 2 cut(s) 628, 890
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 3 cut(s) 329, 387, 503
BstENI CCTNNNNNAGG 2 cut(s) 878, 935
BstF5I GGATG 4 cut(s) 55, 374, 534, 739
BstFNI CGCG 1 cut(s) 468
BstHHI GCGC 1 cut(s) 468
BstKTI GATC 6 cut(s) 13, 55, 193, 453, 457, 805
BstMAI GTCTC 2 cut(s) 159, 752
BstMBI GATC 6 cut(s) 10, 52, 190, 450, 454, 802
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 2 cut(s) 320, 508
BstNI CCWGG 1 cut(s) 932
BstSCI CCNGG 2 cut(s) 847, 930
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 2 cut(s) 274, 655
BstV2I GAAGAC 3 cut(s) 206, 384, 555
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 453
BsuRI GGCC 3 cut(s) 90, 714, 744
BsuTUI ATCGAT 1 cut(s) 453
BtsCI GGATG 4 cut(s) 55, 374, 534, 739
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 1 cut(s) 468
Cfr13I GGNCC 3 cut(s) 89, 713, 826
ClaI ATCGAT 1 cut(s) 453
Csp6I GTAC 3 cut(s) 479, 629, 891
CviAII CATG 2 cut(s) 156, 208
CviQI GTAC 3 cut(s) 479, 629, 891
DdeI CTNAG 3 cut(s) 329, 387, 503
DpnI GATC 6 cut(s) 12, 54, 192, 452, 456, 804
DpnII GATC 6 cut(s) 10, 52, 190, 450, 454, 802
DraI TTTAAA 1 cut(s) 351
Eam1104I CTCTTC 2 cut(s) 683, 897
EarI CTCTTC 2 cut(s) 683, 897
EciI GGCGGA 1 cut(s) 712
Eco130I CCWWGG 3 cut(s) 85, 538, 874
Eco47I GGWCC 1 cut(s) 826
EcoNI CCTNNNNNAGG 2 cut(s) 878, 935
EcoO109I RGGNCCY 2 cut(s) 89, 713
EcoRII CCWGG 1 cut(s) 930
EcoT14I CCWWGG 3 cut(s) 85, 538, 874
ErhI CCWWGG 3 cut(s) 85, 538, 874
FaeI CATG 2 cut(s) 159, 211
FalI AAGNNNNNCTT 2 cut(s) 16, 48
FaqI GGGAC 1 cut(s) 1047
FatI CATG 2 cut(s) 155, 207
FauNDI CATATG 1 cut(s) 585
FbaI TGATCA 3 cut(s) 10, 52, 802
FblI GTMKAC 2 cut(s) 126, 531
Fnu4HI GCNGC 2 cut(s) 263, 644
FokI GGATG 4 cut(s) 42, 361, 521, 746
Fsp4HI GCNGC 2 cut(s) 263, 644
FspBI CTAG 3 cut(s) 494, 525, 717
GlaI GCGC 1 cut(s) 467
GluI GCNGC 2 cut(s) 263, 644
GsuI CTGGAG 2 cut(s) 89, 232
HaeIII GGCC 3 cut(s) 90, 714, 744
HapII CCGG 1 cut(s) 848
HhaI GCGC 1 cut(s) 468
Hin1II CATG 2 cut(s) 159, 211
Hin6I GCGC 1 cut(s) 466
HinP1I GCGC 1 cut(s) 466
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 2 cut(s) 25, 500
HinfI GANTC 5 cut(s) 4, 211, 410, 528, 616
HpaII CCGG 1 cut(s) 848
HphI GGTGA 2 cut(s) 97, 890
Hpy166II GTNNAC 2 cut(s) 127, 532
Hpy188I TCNGA 3 cut(s) 332, 802, 820
Hpy188III TCNNGA 4 cut(s) 8, 14, 361, 1001
Hpy8I GTNNAC 2 cut(s) 127, 532
Hpy99I CGWCG 2 cut(s) 128, 131
HpyAV CCTTC 5 cut(s) 280, 360, 364, 423, 687
HpyCH4IV ACGT 2 cut(s) 870, 910
HpyCH4V TGCA 1 cut(s) 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 508
HpyF3I CTNAG 3 cut(s) 329, 387, 503
HpySE526I ACGT 2 cut(s) 870, 910
Hsp92II CATG 2 cut(s) 159, 211
HspAI GCGC 1 cut(s) 466
Ksp22I TGATCA 3 cut(s) 10, 52, 802
Kzo9I GATC 6 cut(s) 10, 52, 190, 450, 454, 802
LmnI GCTCC 2 cut(s) 1039, 1060
Lsp1109I GCAGC 2 cut(s) 274, 655
LweI GCATC 2 cut(s) 352, 383
MaeI CTAG 3 cut(s) 494, 525, 717
MaeII ACGT 2 cut(s) 870, 910
MalI GATC 6 cut(s) 12, 54, 192, 452, 456, 804
MboI GATC 6 cut(s) 10, 52, 190, 450, 454, 802
MfeI CAATTG 1 cut(s) 635
MflI RGATCY 1 cut(s) 190
MluCI AATT 6 cut(s) 635, 737, 830, 953, 981, 1032
MlyI GAGTC 3 cut(s) 220, 404, 537
MmeI TCCRAC 3 cut(s) 406, 730, 804
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 3 cut(s) 350, 768, 1031
MspI CCGG 1 cut(s) 848
MspR9I CCNGG 2 cut(s) 849, 932
MunI CAATTG 1 cut(s) 635
MvaI CCWGG 1 cut(s) 932
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 320, 508
NciI CCSGG 1 cut(s) 849
NdeI CATATG 1 cut(s) 585
NdeII GATC 6 cut(s) 10, 52, 190, 450, 454, 802
NlaIII CATG 2 cut(s) 159, 211
NlaIV GGNNCC 3 cut(s) 192, 862, 1056
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 2 cut(s) 4, 616
PflFI GACNNNGTC 1 cut(s) 122
PflMI CCANNNNNTGG 1 cut(s) 544
PkrI GCNGC 2 cut(s) 264, 645
PleI GAGTC 3 cut(s) 219, 404, 536
PpsI GAGTC 3 cut(s) 219, 404, 536
Psp6I CCWGG 1 cut(s) 930
PspGI CCWGG 1 cut(s) 930
PspN4I GGNNCC 3 cut(s) 192, 862, 1056
PspPI GGNCC 3 cut(s) 89, 713, 826
PsuI RGATCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 122
RsaI GTAC 3 cut(s) 480, 630, 892
RsaNI GTAC 3 cut(s) 479, 629, 891
SalI GTCGAC 1 cut(s) 125
SaqAI TTAA 3 cut(s) 350, 768, 1031
SatI GCNGC 2 cut(s) 263, 644
Sau3AI GATC 6 cut(s) 10, 52, 190, 450, 454, 802
Sau96I GGNCC 3 cut(s) 89, 713, 826
SchI GAGTC 3 cut(s) 220, 404, 537
ScrFI CCNGG 2 cut(s) 849, 932
SfaNI GCATC 2 cut(s) 352, 383
SgrDI CGTCGACG 1 cut(s) 125
SinI GGWCC 1 cut(s) 826
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 6 cut(s) 635, 737, 830, 953, 981, 1032
SsiI CCGC 4 cut(s) 44, 697, 779, 858
SspI AATATT 1 cut(s) 796
SspMI CTAG 3 cut(s) 494, 525, 717
StyD4I CCNGG 2 cut(s) 847, 930
StyI CCWWGG 3 cut(s) 85, 538, 874
TaiI ACGT 2 cut(s) 873, 913
TaqI TCGA 4 cut(s) 126, 453, 960, 1023
TaqII GACCGA 1 cut(s) 665
TasI AATT 6 cut(s) 635, 737, 830, 953, 981, 1032
TatI WGTACW 2 cut(s) 628, 890
TfiI GAWTC 2 cut(s) 4, 616
Tru1I TTAA 3 cut(s) 350, 768, 1031
Tru9I TTAA 3 cut(s) 350, 768, 1031
TscAI CASTG 1 cut(s) 72
TseI GCWGC 2 cut(s) 262, 643
TspDTI ATGAA 6 cut(s) 17, 144, 333, 587, 750, 990
TspRI CASTG 1 cut(s) 72
Tth111I GACNNNGTC 1 cut(s) 122
Van91I CCANNNNNTGG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 826
XagI CCTNNNNNAGG 2 cut(s) 878, 935
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
XmiI GTMKAC 2 cut(s) 126, 531
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 3 cut(s) 494, 525, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.