FvH4_4g03440

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014022
Physical Location & Seq
Reverse (-)
3084270 .. 3085379
1110 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03440.t1

Sequence Viewer

Length: 1110 bp
ATGGATCTTGTAAGAAAAGGCTACATGATCCATGTCTCTTCATCAGAAGTACAAGCACTTGCTCCAATTACAAGTCCAGGGAGCTCAATCTTTGGGACTCCTATTCATCATTCAGACACAAGCTTCCCCATTATAGCAATTGCCATAATAGGAATTATAGCCACAGCTTTCTTGCTAGTGAGCTACTACATCTTTGTCATCAAGTGCTGCCTCAACTGGCACCGTGTGGATCTCCTCCGGCGATTATCGCTTTCCCGGAACCGGCGAGACGAAAACCAGCTAATGGTGTACTCGCCGGGTTTGGAGACCCGGGGGCTCGACGAAGCTGTGATCAGGTCAATCCCATTGTTGCAGTTCAAGAAGGATGGGAAAAACAGAGGTGGGTTTGAAGACGGAAGCCTATTTTGTGAGTGTGCTGTTTGCTTGAATGAGTTTCAAGAAGATGAGAAGCTGAGGATCATACCCAACTGCAGCCATGTCTTCCACATTGACTGCATTGACGTTTGGCTTCAAAACAATGCGAATTGCCCGCTTTGTAGAACAAGCATTTCATCCAACACAACCAGGTTCCCATACGATCGAATCGTGGCTCCGAGCTCTTCACCTCAAATTCCAACCCCTGGGAGTGCCAATGGAGGCGATGAAGACTATGTGGTGATCGAACTGAGCAACGAAAACAATCCGGCGGATCAAGCATTGATGCTTGGAGTGCAAGAAAGATTGAACTCAGGGGAGTTGTCAGTGAGGTCCATTAGTCCTTCCCCAAGGAAATTGGAACAAATTAGAACATTTCTGCCCAAGAAGGCAAGAAAGTTTAACAACAAGGTTACTAGTATGGGTGATGAGTGCATTGACATTAGGGGAAAAGATGAGCAATTTTCCATCCAACCCATCAGGAGGTCTTTCTCAATGGACTCTTCTAATGACAGACATCTCTATTTGGCAGTTCAAGAAGCTGTGCAGCAGCAAAGCCAAGTCAGTGAGGTTAGTCCAATTGAAGGTTGCAGCAGTAGTACCAGTTCTAGAATCAGAAGGTCTTTCTTTTCTTTTGGGCATGGTAGGGGATCTAGAAGTAACAATGCAATTCTGCCTGTTTATTTGGAGCCTTAA

Protein Analysis

370

Amino Acids

41.22

Weight (kDa)

7.06

Isoelectric Point (pI)

59.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 137 - 180 7.1e-13 Ring finger domain
zf-RING_11 PF17123 137 - 166 1e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 137 - 179 3.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 137 - 181 3.4e-06 zinc-RING finger domain
zf-C3HC4 PF00097 138 - 179 5e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 146 - 180 9.5e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43420
fragaria_vesca FvH4_4g03440
malus_domestica MD13G1212600.v1.1 MD16G1216300.v1.1
prunus_persica Prupe.1G037600_v2.0.a1
pyrus_communis pycom13g18440 pycom16g18090
rosa_chinensis RchiOBHm_Chr4g0392431
rosa_laevigata RLG00000009807
rosa_multiflora Rmu_sc0001800.1_g000001
rosa_roxburghii Rroxscaffold_5G00337650
rosa_rugosa Rorug03G0338800
rosa_samantha Rh4AG042800 Rh4BG037800 Rh4CG045800 Rh4DG039900
rosa_wichuraiana Rw4G003340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 219
AccB7I CCANNNNNTGG 2 cut(s) 283, 620
AciI CCGC 2 cut(s) 530, 686
AclWI GGATC 6 cut(s) 12, 22, 237, 464, 696, 1072
AcsI RAATTY 1 cut(s) 609
AdeI CACNNNGTG 1 cut(s) 226
AfaI GTAC 3 cut(s) 51, 290, 1015
AfiI CCNNNNNNNGG 5 cut(s) 261, 283, 620, 897, 998
AgsI TTSAA 8 cut(s) 358, 389, 427, 437, 512, 724, 950, 998
AhlI ACTAGT 1 cut(s) 830
AjnI CCWGG 3 cut(s) 76, 563, 619
AluBI AGCT 9 cut(s) 84, 123, 167, 183, 280, 326, 451, 597, 956
AluI AGCT 9 cut(s) 84, 123, 167, 183, 280, 326, 451, 597, 956
Alw21I GWGCWC 2 cut(s) 86, 599
Alw26I GTCTC 3 cut(s) 40, 261, 299
AlwI GGATC 6 cut(s) 12, 22, 237, 464, 696, 1072
Ama87I CYCGRG 1 cut(s) 309
ApeKI GCWGC 5 cut(s) 207, 471, 961, 964, 1005
ApoI RAATTY 1 cut(s) 609
AspS9I GGNCC 1 cut(s) 747
AsuC2I CCSGG 4 cut(s) 256, 297, 310, 311
AsuHPI GGTGA 3 cut(s) 594, 667, 851
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 1 cut(s) 747
BanI GGYRCC 1 cut(s) 219
BanII GRGCYC 3 cut(s) 86, 318, 599
BbsI GAAGAC 3 cut(s) 396, 472, 651
Bbv12I GWGCWC 2 cut(s) 86, 599
BbvCI CCTCAGC 1 cut(s) 452
BbvI GCAGC 5 cut(s) 194, 483, 973, 976, 1017
BccI CCATC 3 cut(s) 359, 890, 899
BciT130I CCWGG 3 cut(s) 78, 565, 621
BclI TGATCA 1 cut(s) 330
BcnI CCSGG 4 cut(s) 256, 297, 310, 311
BcoDI GTCTC 3 cut(s) 40, 261, 299
BcuI ACTAGT 1 cut(s) 830
BfaI CTAG 4 cut(s) 176, 831, 1023, 1068
BfmI CTRYAG 1 cut(s) 469
BisI GCNGC 5 cut(s) 208, 472, 962, 965, 1006
BlsI GCNGC 5 cut(s) 209, 473, 963, 966, 1007
Bme1390I CCNGG 7 cut(s) 78, 256, 297, 310, 311, 565, 621
Bme18I GGWCC 1 cut(s) 747
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 747
BmiI GGNNCC 5 cut(s) 221, 260, 569, 591, 1104
BmrFI CCNGG 7 cut(s) 78, 256, 297, 310, 311, 565, 621
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 3 cut(s) 396, 472, 651
Bpu10I CCTNAGC 1 cut(s) 452
BpuMI CCSGG 4 cut(s) 256, 297, 310, 311
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 6 cut(s) 77, 309, 310, 619, 620, 764
Bsc4I CCNNNNNNNGG 5 cut(s) 261, 283, 620, 897, 998
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 2 cut(s) 221, 1017
BseBI CCWGG 3 cut(s) 78, 565, 621
BseDI CCNNGG 6 cut(s) 77, 309, 310, 619, 620, 764
BseGI GGATG 3 cut(s) 370, 551, 882
BseLI CCNNNNNNNGG 5 cut(s) 261, 283, 620, 897, 998
BseMII CTCAG 3 cut(s) 443, 656, 741
BseNI ACTGG 2 cut(s) 221, 1017
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 5 cut(s) 194, 483, 973, 976, 1017
BsgI GTGCAG 1 cut(s) 980
Bsh1285I CGRYCG 1 cut(s) 580
BshNI GGYRCC 1 cut(s) 219
BsiEI CGRYCG 1 cut(s) 580
BsiHKAI GWGCWC 2 cut(s) 86, 599
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 6 cut(s) 238, 256, 262, 296, 310, 683
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 5 cut(s) 261, 283, 620, 897, 998
BsmAI GTCTC 3 cut(s) 40, 261, 299
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 1 cut(s) 109
Bso31I GGTCTC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 3 cut(s) 86, 318, 599
Bsp143I GATC 9 cut(s) 4, 27, 229, 330, 456, 577, 657, 688, 1064
BspACI CCGC 2 cut(s) 530, 686
BspCNI CTCAG 3 cut(s) 444, 657, 740
BspLI GGNNCC 5 cut(s) 221, 260, 569, 591, 1104
BspMAI CTGCAG 1 cut(s) 473
BspPI GGATC 6 cut(s) 12, 22, 237, 464, 696, 1072
BspQI GCTCTTC 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 219
BspTNI GGTCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 2 cut(s) 221, 1017
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 6 cut(s) 77, 309, 310, 619, 620, 764
BssMI GATC 9 cut(s) 4, 27, 229, 330, 456, 577, 657, 688, 1064
BssT1I CCWWGG 1 cut(s) 764
Bst2UI CCWGG 3 cut(s) 78, 565, 621
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 3 cut(s) 43, 604, 922
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 3 cut(s) 452, 665, 727
BstF5I GGATG 3 cut(s) 370, 551, 882
BstKTI GATC 9 cut(s) 7, 30, 232, 333, 459, 580, 660, 691, 1067
BstMAI GTCTC 3 cut(s) 40, 261, 299
BstMBI GATC 9 cut(s) 4, 27, 229, 330, 456, 577, 657, 688, 1064
BstMCI CGRYCG 1 cut(s) 580
BstMWI GCNNNNNNNGC 3 cut(s) 247, 692, 709
BstNI CCWGG 3 cut(s) 78, 565, 621
BstSCI CCNGG 7 cut(s) 76, 254, 295, 308, 309, 563, 619
BstSFI CTRYAG 1 cut(s) 469
BstV1I GCAGC 5 cut(s) 194, 483, 973, 976, 1017
BstV2I GAAGAC 3 cut(s) 396, 472, 651
BstX2I RGATCY 3 cut(s) 4, 229, 1064
BstYI RGATCY 3 cut(s) 4, 229, 1064
BtgZI GCGATG 1 cut(s) 654
BtsCI GGATG 3 cut(s) 370, 551, 882
BtsIMutI CAGTG 2 cut(s) 747, 985
Cac8I GCNNGC 1 cut(s) 530
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 747
Cfr9I CCCGGG 1 cut(s) 309
CsiI ACCWGGT 1 cut(s) 563
Csp6I GTAC 3 cut(s) 50, 289, 1014
CviAII CATG 4 cut(s) 25, 32, 476, 1055
CviQI GTAC 3 cut(s) 50, 289, 1014
DdeI CTNAG 3 cut(s) 452, 665, 727
DpnI GATC 9 cut(s) 6, 29, 231, 332, 458, 579, 659, 690, 1066
DpnII GATC 9 cut(s) 4, 27, 229, 330, 456, 577, 657, 688, 1064
DraIII CACNNNGTG 1 cut(s) 226
Eam1104I CTCTTC 3 cut(s) 43, 604, 922
EarI CTCTTC 3 cut(s) 43, 604, 922
EciI GGCGGA 1 cut(s) 701
Ecl136II GAGCTC 2 cut(s) 84, 597
Eco130I CCWWGG 1 cut(s) 764
Eco24I GRGCYC 3 cut(s) 86, 318, 599
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 747
Eco53kI GAGCTC 2 cut(s) 84, 597
Eco88I CYCGRG 1 cut(s) 309
EcoICRI GAGCTC 2 cut(s) 84, 597
EcoRII CCWGG 3 cut(s) 76, 563, 619
EcoT14I CCWWGG 1 cut(s) 764
EcoT38I GRGCYC 3 cut(s) 86, 318, 599
ErhI CCWWGG 1 cut(s) 764
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 4 cut(s) 28, 35, 479, 1058
FaqI GGGAC 1 cut(s) 109
FatI CATG 4 cut(s) 24, 31, 475, 1054
FauI CCCGC 1 cut(s) 537
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 5 cut(s) 208, 472, 962, 965, 1006
FokI GGATG 3 cut(s) 377, 538, 869
FriOI GRGCYC 3 cut(s) 86, 318, 599
Fsp4HI GCNGC 5 cut(s) 208, 472, 962, 965, 1006
FspBI CTAG 4 cut(s) 176, 831, 1023, 1068
GluI GCNGC 5 cut(s) 208, 472, 962, 965, 1006
HapII CCGG 6 cut(s) 238, 256, 262, 296, 310, 683
Hin1II CATG 4 cut(s) 28, 35, 479, 1058
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 4 cut(s) 97, 582, 914, 1026
HpaII CCGG 6 cut(s) 238, 256, 262, 296, 310, 683
HphI GGTGA 3 cut(s) 594, 667, 851
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 4 cut(s) 46, 115, 594, 1031
Hpy188III TCNNGA 6 cut(s) 358, 437, 895, 950, 1023, 1068
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 5 cut(s) 355, 768, 796, 992, 1026
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 1 cut(s) 501
HpyCH4V TGCA 8 cut(s) 352, 471, 495, 712, 849, 961, 1005, 1082
HpyF10VI GCNNNNNNNGC 3 cut(s) 247, 692, 709
HpyF3I CTNAG 3 cut(s) 452, 665, 727
HpySE526I ACGT 1 cut(s) 501
Hsp92II CATG 4 cut(s) 28, 35, 479, 1058
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 9 cut(s) 4, 27, 229, 330, 456, 577, 657, 688, 1064
LguI GCTCTTC 1 cut(s) 604
LmnI GCTCC 4 cut(s) 67, 81, 595, 1102
Lsp1109I GCAGC 5 cut(s) 194, 483, 973, 976, 1017
LweI GCATC 1 cut(s) 690
MabI ACCWGGT 1 cut(s) 563
MaeI CTAG 4 cut(s) 176, 831, 1023, 1068
MaeII ACGT 1 cut(s) 501
MaeIII GTNAC 2 cut(s) 826, 1073
MalI GATC 9 cut(s) 6, 29, 231, 332, 458, 579, 659, 690, 1066
MboI GATC 9 cut(s) 4, 27, 229, 330, 456, 577, 657, 688, 1064
MboII GAAGA 7 cut(s) 30, 401, 452, 472, 591, 656, 909
MfeI CAATTG 2 cut(s) 138, 993
MflI RGATCY 3 cut(s) 4, 229, 1064
MhlI GDGCHC 3 cut(s) 86, 318, 599
MlyI GAGTC 2 cut(s) 91, 908
MmeI TCCRAC 3 cut(s) 579, 638, 910
MnlI CCTC 9 cut(s) 221, 245, 371, 447, 615, 629, 738, 891, 976
MseI TTAA 2 cut(s) 816, 1108
MspI CCGG 6 cut(s) 238, 256, 262, 296, 310, 683
MspR9I CCNGG 7 cut(s) 78, 256, 297, 310, 311, 565, 621
MunI CAATTG 2 cut(s) 138, 993
MvaI CCWGG 3 cut(s) 78, 565, 621
MwoI GCNNNNNNNGC 3 cut(s) 247, 692, 709
NciI CCSGG 4 cut(s) 256, 297, 310, 311
NdeII GATC 9 cut(s) 4, 27, 229, 330, 456, 577, 657, 688, 1064
NlaIII CATG 4 cut(s) 28, 35, 479, 1058
NlaIV GGNNCC 5 cut(s) 221, 260, 569, 591, 1104
PasI CCCWGGG 1 cut(s) 620
PciSI GCTCTTC 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PfeI GAWTC 2 cut(s) 582, 1026
PflMI CCANNNNNTGG 2 cut(s) 283, 620
PfoI TCCNGGA 1 cut(s) 254
PkrI GCNGC 5 cut(s) 209, 473, 963, 966, 1007
Ple19I CGATCG 1 cut(s) 580
PleI GAGTC 2 cut(s) 91, 908
PpsI GAGTC 2 cut(s) 91, 908
Psp124BI GAGCTC 2 cut(s) 86, 599
Psp6I CCWGG 3 cut(s) 76, 563, 619
PspGI CCWGG 3 cut(s) 76, 563, 619
PspN4I GGNNCC 5 cut(s) 221, 260, 569, 591, 1104
PspPI GGNCC 1 cut(s) 747
PsrI GAACNNNNNNTAC 2 cut(s) 1003, 1035
PstI CTGCAG 1 cut(s) 473
PsuI RGATCY 3 cut(s) 4, 229, 1064
PvuI CGATCG 1 cut(s) 580
RsaI GTAC 3 cut(s) 51, 290, 1015
RsaNI GTAC 3 cut(s) 50, 289, 1014
SacI GAGCTC 2 cut(s) 86, 599
SapI GCTCTTC 1 cut(s) 604
SaqAI TTAA 2 cut(s) 816, 1108
SatI GCNGC 5 cut(s) 208, 472, 962, 965, 1006
Sau3AI GATC 9 cut(s) 4, 27, 229, 330, 456, 577, 657, 688, 1064
Sau96I GGNCC 1 cut(s) 747
SchI GAGTC 2 cut(s) 91, 908
ScrFI CCNGG 7 cut(s) 78, 256, 297, 310, 311, 565, 621
SduI GDGCHC 3 cut(s) 86, 318, 599
SexAI ACCWGGT 1 cut(s) 563
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 747
SmaI CCCGGG 1 cut(s) 311
SpeI ACTAGT 1 cut(s) 830
SsiI CCGC 2 cut(s) 530, 686
SspMI CTAG 4 cut(s) 176, 831, 1023, 1068
SstI GAGCTC 2 cut(s) 86, 599
StyD4I CCNGG 7 cut(s) 76, 254, 295, 308, 309, 563, 619
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 1 cut(s) 504
TaqI TCGA 3 cut(s) 318, 580, 660
TatI WGTACW 2 cut(s) 49, 288
TfiI GAWTC 2 cut(s) 582, 1026
Tru1I TTAA 2 cut(s) 816, 1108
Tru9I TTAA 2 cut(s) 816, 1108
TscAI CASTG 2 cut(s) 747, 985
TseI GCWGC 5 cut(s) 207, 471, 961, 964, 1005
TspDTI ATGAA 4 cut(s) 30, 95, 540, 657
TspGWI ACGGA 1 cut(s) 408
TspMI CCCGGG 1 cut(s) 309
TspRI CASTG 2 cut(s) 747, 985
Van91I CCANNNNNTGG 2 cut(s) 283, 620
VpaK11BI GGWCC 1 cut(s) 747
XapI RAATTY 1 cut(s) 609
XbaI TCTAGA 2 cut(s) 1022, 1067
XmaI CCCGGG 1 cut(s) 309
XspI CTAG 4 cut(s) 176, 831, 1023, 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.