Rh4AG042800

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014022
Physical Location & Seq
Reverse (-)
8845419 .. 8866717
21299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG042800.1

Sequence Viewer

Length: 1326 bp
ATGGATCTTGTAAGAAAAGGCTACGTGATTCATGTCTCTTCATCAGAAGCACAAGCACTTGCGCCAATTACAAGTCCAGGAAGCTCAATCTTTGGGACTCCTATTCACCATTCAGACACAAGCTTCCCCATTATAGCAATTGCCATAATAGGAATTATAGCCACGGCTTTCTTGCTAGTGAGCTACTACATCTTTGTCATCAAGTGCTGCCTCAACTGGCACCGTGTGGATCTCCTCAGGCGATTTTCACTGTCACGAAACCGGCGCGACGAAGACCAGCTAATGGTGTACTCGCCGGGTTTGGAGACCCGAGGGCTCGATGAAGCTGTGATCAGATCAATCCCATTGTTGCAGTTCAAGAAAGAAGGGAAGAACAGAGGTGGGTTTGAACAAGGAAGCTTGTTTTGTGAGTGTGCTGTTTGCTTGAATGAGTTTCAAGAAGATGAGAAGCTGAGGATCATACCCAACTGCAGCCATGTTTTCCACATTGACTGCATTGACGTTTGGCTTCAAAACAACGCGAATTGCCCGCTTTGTAGAACAAGCATTTCATCCAACACAACCCGGTTCCAATTCGATCAAATCGTGGCTCCGAGCTCTTCACCTCAAAATCCAACCCCTGGGAGTCTCGATGGAGGCGACGACGACTTCGTGGTGATCGAATTGAGCAACGACAACAATCCGGCGGATCAAGCGTTGATGCTTGGAGTGCAAGAAAAATTGAACTCAGGGGAGTTGTCAGTGAGGTCCATTAGTCCTTCCCCAAGGAAATTGGAACAAATTAGAACATTTGTGCCCAAAAAGGCAAGAAAGTTTCACAACAAGGTTACTAGTATGGGTGATGAGTGCATTGACATTAGGGGCAAAGATGAGCAATTCTCCATCCAACCCATAAGGAGGTCTTTCTCTATGGACTCATCTAATGATAGACAGCTCTATTTGGCAGTTCAAGAAGCTGTGCAGCAGCAAAGCCAAGTCAGTGAGAACTGGTTCCCTTGGCTGGGTTTGAGTGTTCGCAGGCACGAGTGCAAACAATTTCTTGCTGGTCTACTTGAGCGTGGCAGACGTACACAACAACAATTAATGGACAGAGCCAATCAGAAATCCAAATCAAATAGCAGTCAAGTTCAGAATGCTTCTCAAGATAACTTTGTTAGACTTGTGACTGCTCGTTTCGTTGAACGTTGCCCAGCCCCTGAACCACTTCTTGAAGAAGCACCTGAACAAGAAGGTCCAAAGAAGCCATGCTCTAAATCTGCTAAGATGTTCAGAATCAATTGCCAACTACCAGTTGATGATCGACTAGTTAAGGACATCAAAGACTGA

Protein Analysis

441

Amino Acids

49.79

Weight (kDa)

7.5

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 137 - 180 9e-13 Ring finger domain
zf-RING_11 PF17123 137 - 166 1.3e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 137 - 179 4.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 137 - 181 4.3e-06 zinc-RING finger domain
zf-C3HC4 PF00097 138 - 179 6.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 146 - 180 1.2e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43420
fragaria_vesca FvH4_4g03440
malus_domestica MD13G1212600.v1.1 MD16G1216300.v1.1
prunus_persica Prupe.1G037600_v2.0.a1
pyrus_communis pycom13g18440 pycom16g18090
rosa_chinensis RchiOBHm_Chr4g0392431
rosa_laevigata RLG00000009807
rosa_multiflora Rmu_sc0001800.1_g000001
rosa_roxburghii Rroxscaffold_5G00337650
rosa_rugosa Rorug03G0338800
rosa_samantha Rh4AG042800 Rh4BG037800 Rh4CG045800 Rh4DG039900
rosa_wichuraiana Rw4G003340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 219
AccB7I CCANNNNNTGG 2 cut(s) 283, 620
AccI GTMKAC 1 cut(s) 1048
AccII CGCG 2 cut(s) 267, 521
AciI CCGC 2 cut(s) 530, 686
AclI AACGTT 1 cut(s) 1183
AclWI GGATC 4 cut(s) 12, 237, 464, 696
AdeI CACNNNGTG 1 cut(s) 226
AfaI GTAC 2 cut(s) 290, 1069
AfiI CCNNNNNNNGG 5 cut(s) 283, 620, 897, 1000, 1001
AgsI TTSAA 9 cut(s) 358, 389, 427, 437, 512, 724, 950, 1181, 1211
AhlI ACTAGT 2 cut(s) 830, 1303
AjnI CCWGG 2 cut(s) 76, 619
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 3 cut(s) 40, 299, 632
AlwI GGATC 4 cut(s) 12, 237, 464, 696
AlwNI CAGNNNCTG 1 cut(s) 1196
Ama87I CYCGRG 1 cut(s) 309
ApeKI GCWGC 4 cut(s) 207, 471, 961, 964
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AseI ATTAAT 1 cut(s) 1082
Asp700I GAANNNNTTC 2 cut(s) 989, 1203
AspLEI GCGC 2 cut(s) 64, 267
AspS9I GGNCC 2 cut(s) 747, 1232
AsuC2I CCSGG 2 cut(s) 297, 565
AsuHPI GGTGA 4 cut(s) 98, 594, 667, 851
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 2 cut(s) 747, 1232
AxyI CCTNAGG 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 798
BanI GGYRCC 1 cut(s) 219
BanII GRGCYC 2 cut(s) 318, 599
BauI CACGAG 1 cut(s) 1022
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 599
BbvCI CCTCAGC 1 cut(s) 452
BbvI GCAGC 4 cut(s) 194, 483, 973, 976
BccI CCATC 2 cut(s) 626, 890
BceAI ACGGC 1 cut(s) 180
BciT130I CCWGG 2 cut(s) 78, 621
BclI TGATCA 1 cut(s) 330
BcnI CCSGG 2 cut(s) 297, 565
BcoDI GTCTC 3 cut(s) 40, 299, 632
BcuI ACTAGT 2 cut(s) 830, 1303
BfaI CTAG 3 cut(s) 176, 831, 1304
BfmI CTRYAG 1 cut(s) 469
BisI GCNGC 4 cut(s) 208, 472, 962, 965
BlsI GCNGC 4 cut(s) 209, 473, 963, 966
Bme1390I CCNGG 4 cut(s) 78, 297, 565, 621
Bme18I GGWCC 2 cut(s) 747, 1232
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 2 cut(s) 747, 1232
BmiI GGNNCC 4 cut(s) 221, 569, 591, 992
BmrFI CCNGG 4 cut(s) 78, 297, 565, 621
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 279
BplI GAGNNNNNCTC 2 cut(s) 863, 895
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 2 cut(s) 1073, 1125
BpuMI CCSGG 2 cut(s) 297, 565
BsaAI YACGTR 1 cut(s) 25
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 6 cut(s) 162, 310, 619, 620, 764, 995
Bsc4I CCNNNNNNNGG 5 cut(s) 283, 620, 897, 1000, 1001
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 3 cut(s) 221, 992, 1289
Bse21I CCTNAGG 1 cut(s) 236
BseBI CCWGG 2 cut(s) 78, 621
BseDI CCNNGG 6 cut(s) 162, 310, 619, 620, 764, 995
BseGI GGATG 2 cut(s) 551, 882
BseLI CCNNNNNNNGG 5 cut(s) 283, 620, 897, 1000, 1001
BseMII CTCAG 3 cut(s) 250, 443, 741
BseNI ACTGG 3 cut(s) 221, 992, 1289
BseRI GAGGAG 1 cut(s) 224
BseSI GKGCMC 1 cut(s) 798
BseXI GCAGC 4 cut(s) 194, 483, 973, 976
BseYI CCCAGC 2 cut(s) 1000, 1189
BsgI GTGCAG 1 cut(s) 980
Bsh1236I CGCG 2 cut(s) 267, 521
BshNI GGYRCC 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 599
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 4 cut(s) 262, 296, 565, 683
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 5 cut(s) 283, 620, 897, 1000, 1001
BsmAI GTCTC 3 cut(s) 40, 299, 632
BsmFI GGGAC 1 cut(s) 109
BsmI GAATGC 1 cut(s) 1138
Bso31I GGTCTC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 3 cut(s) 318, 599, 798
Bsp143I GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1297
BspACI CCGC 2 cut(s) 530, 686
BspCNI CTCAG 3 cut(s) 249, 444, 740
BspFNI CGCG 2 cut(s) 267, 521
BspLI GGNNCC 4 cut(s) 221, 569, 591, 992
BspMAI CTGCAG 1 cut(s) 473
BspPI GGATC 4 cut(s) 12, 237, 464, 696
BspQI GCTCTTC 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 219
BspTNI GGTCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 3 cut(s) 221, 992, 1289
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 6 cut(s) 162, 310, 619, 620, 764, 995
BssMI GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1297
BssSI CACGAG 1 cut(s) 1022
BssT1I CCWWGG 2 cut(s) 764, 995
Bst2BI CACGAG 1 cut(s) 1022
Bst2UI CCWGG 2 cut(s) 78, 621
Bst4CI ACNGT 2 cut(s) 224, 252
Bst6I CTCTTC 2 cut(s) 43, 604
BstBAI YACGTR 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 530, 1019
BstDEI CTNAG 4 cut(s) 236, 452, 727, 1260
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 2 cut(s) 551, 882
BstFNI CGCG 2 cut(s) 267, 521
BstHHI GCGC 2 cut(s) 64, 267
BstKTI GATC 9 cut(s) 7, 232, 333, 338, 459, 580, 660, 691, 1300
BstMAI GTCTC 3 cut(s) 40, 299, 632
BstMBI GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1297
BstMWI GCNNNNNNNGC 2 cut(s) 692, 709
BstNI CCWGG 2 cut(s) 78, 621
BstSCI CCNGG 4 cut(s) 76, 295, 563, 619
BstSFI CTRYAG 1 cut(s) 469
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 2 cut(s) 267, 521
BstV1I GCAGC 4 cut(s) 194, 483, 973, 976
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 2 cut(s) 4, 229
BstYI RGATCY 2 cut(s) 4, 229
Bsu36I CCTNAGG 1 cut(s) 236
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 2 cut(s) 551, 882
BtsIMutI CAGTG 3 cut(s) 248, 747, 985
Cac8I GCNNGC 2 cut(s) 530, 1019
CaiI CAGNNNCTG 1 cut(s) 1196
CfoI GCGC 2 cut(s) 64, 267
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 2 cut(s) 747, 1232
Csp6I GTAC 2 cut(s) 289, 1068
CviAII CATG 3 cut(s) 32, 476, 1245
CviQI GTAC 2 cut(s) 289, 1068
DdeI CTNAG 4 cut(s) 236, 452, 727, 1260
DpnI GATC 9 cut(s) 6, 231, 332, 337, 458, 579, 659, 690, 1299
DpnII GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1297
DraIII CACNNNGTG 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 43, 604
EarI CTCTTC 2 cut(s) 43, 604
EciI GGCGGA 1 cut(s) 701
Ecl136II GAGCTC 1 cut(s) 597
Eco130I CCWWGG 2 cut(s) 764, 995
Eco24I GRGCYC 2 cut(s) 318, 599
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 2 cut(s) 747, 1232
Eco53kI GAGCTC 1 cut(s) 597
Eco81I CCTNAGG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 597
EcoRII CCWGG 2 cut(s) 76, 619
EcoT14I CCWWGG 2 cut(s) 764, 995
EcoT38I GRGCYC 2 cut(s) 318, 599
ErhI CCWWGG 2 cut(s) 764, 995
FaeI CATG 3 cut(s) 35, 479, 1248
FalI AAGNNNNNCTT 2 cut(s) 886, 918
FaqI GGGAC 1 cut(s) 109
FatI CATG 3 cut(s) 31, 475, 1244
FauI CCCGC 1 cut(s) 537
FbaI TGATCA 1 cut(s) 330
FblI GTMKAC 1 cut(s) 1048
Fnu4HI GCNGC 4 cut(s) 208, 472, 962, 965
FokI GGATG 2 cut(s) 538, 869
FriOI GRGCYC 2 cut(s) 318, 599
Fsp4HI GCNGC 4 cut(s) 208, 472, 962, 965
FspBI CTAG 3 cut(s) 176, 831, 1304
GlaI GCGC 2 cut(s) 63, 266
GluI GCNGC 4 cut(s) 208, 472, 962, 965
GsaI CCCAGC 2 cut(s) 1004, 1193
HapII CCGG 4 cut(s) 262, 296, 565, 683
HhaI GCGC 2 cut(s) 64, 267
Hin1II CATG 3 cut(s) 35, 479, 1248
Hin6I GCGC 2 cut(s) 62, 265
HinP1I GCGC 2 cut(s) 62, 265
HindIII AAGCTT 2 cut(s) 121, 397
HinfI GANTC 5 cut(s) 28, 97, 625, 914, 1272
HpaII CCGG 4 cut(s) 262, 296, 565, 683
HphI GGTGA 4 cut(s) 98, 594, 667, 851
Hpy166II GTNNAC 3 cut(s) 289, 1049, 1070
Hpy188I TCNGA 7 cut(s) 46, 115, 335, 594, 1101, 1131, 1271
Hpy188III TCNNGA 7 cut(s) 255, 358, 437, 629, 950, 1142, 1208
Hpy8I GTNNAC 3 cut(s) 289, 1049, 1070
Hpy99I CGWCG 3 cut(s) 272, 644, 647
HpyAV CCTTC 3 cut(s) 359, 768, 1223
HpyCH4III ACNGT 2 cut(s) 224, 252
HpyCH4IV ACGT 4 cut(s) 24, 501, 1066, 1183
HpyCH4V TGCA 7 cut(s) 352, 471, 495, 712, 849, 961, 1029
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 709
HpyF3I CTNAG 4 cut(s) 236, 452, 727, 1260
HpySE526I ACGT 4 cut(s) 24, 501, 1066, 1183
Hsp92II CATG 3 cut(s) 35, 479, 1248
HspAI GCGC 2 cut(s) 62, 265
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1297
LguI GCTCTTC 1 cut(s) 604
LmnI GCTCC 1 cut(s) 595
Lsp1109I GCAGC 4 cut(s) 194, 483, 973, 976
LweI GCATC 1 cut(s) 690
MaeI CTAG 3 cut(s) 176, 831, 1304
MaeII ACGT 4 cut(s) 24, 501, 1066, 1183
MaeIII GTNAC 3 cut(s) 252, 826, 1162
MalI GATC 9 cut(s) 6, 231, 332, 337, 458, 579, 659, 690, 1299
MboI GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1297
MboII GAAGA 6 cut(s) 30, 284, 382, 452, 591, 1223
MfeI CAATTG 2 cut(s) 138, 1276
MflI RGATCY 2 cut(s) 4, 229
MhlI GDGCHC 3 cut(s) 318, 599, 798
MlyI GAGTC 3 cut(s) 91, 634, 908
MmeI TCCRAC 3 cut(s) 579, 638, 910
MnlI CCTC 9 cut(s) 221, 245, 305, 371, 447, 615, 629, 738, 891
MroXI GAANNNNTTC 2 cut(s) 989, 1203
MseI TTAA 2 cut(s) 1082, 1308
MspI CCGG 4 cut(s) 262, 296, 565, 683
MspR9I CCNGG 4 cut(s) 78, 297, 565, 621
MunI CAATTG 2 cut(s) 138, 1276
Mva1269I GAATGC 1 cut(s) 1138
MvaI CCWGG 2 cut(s) 78, 621
MvnI CGCG 2 cut(s) 267, 521
MwoI GCNNNNNNNGC 2 cut(s) 692, 709
NciI CCSGG 2 cut(s) 297, 565
NdeII GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1297
NlaIII CATG 3 cut(s) 35, 479, 1248
NlaIV GGNNCC 4 cut(s) 221, 569, 591, 992
NmuCI GTSAC 2 cut(s) 252, 1162
PasI CCCWGGG 1 cut(s) 620
PciSI GCTCTTC 1 cut(s) 604
PcsI WCGNNNNNNNCGW 4 cut(s) 582, 636, 648, 657
PctI GAATGC 1 cut(s) 1138
PdmI GAANNNNTTC 2 cut(s) 989, 1203
PfeI GAWTC 2 cut(s) 28, 1272
PflMI CCANNNNNTGG 2 cut(s) 283, 620
PfoI TCCNGGA 1 cut(s) 76
PkrI GCNGC 4 cut(s) 209, 473, 963, 966
PleI GAGTC 3 cut(s) 91, 633, 908
PpsI GAGTC 3 cut(s) 91, 633, 908
Ppu21I YACGTR 1 cut(s) 25
PshBI ATTAAT 1 cut(s) 1082
Psp124BI GAGCTC 1 cut(s) 599
Psp1406I AACGTT 1 cut(s) 1183
Psp6I CCWGG 2 cut(s) 76, 619
PspFI CCCAGC 2 cut(s) 1000, 1189
PspGI CCWGG 2 cut(s) 76, 619
PspN4I GGNNCC 4 cut(s) 221, 569, 591, 992
PspPI GGNCC 2 cut(s) 747, 1232
PstI CTGCAG 1 cut(s) 473
PstNI CAGNNNCTG 1 cut(s) 1196
PsuI RGATCY 2 cut(s) 4, 229
RsaI GTAC 2 cut(s) 290, 1069
RsaNI GTAC 2 cut(s) 289, 1068
SacI GAGCTC 1 cut(s) 599
SapI GCTCTTC 1 cut(s) 604
SaqAI TTAA 2 cut(s) 1082, 1308
SatI GCNGC 4 cut(s) 208, 472, 962, 965
Sau3AI GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1297
Sau96I GGNCC 2 cut(s) 747, 1232
SchI GAGTC 3 cut(s) 91, 634, 908
ScrFI CCNGG 4 cut(s) 78, 297, 565, 621
SduI GDGCHC 3 cut(s) 318, 599, 798
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 2 cut(s) 747, 1232
SmlI CTYRAG 2 cut(s) 1052, 1140
SmoI CTYRAG 2 cut(s) 1052, 1140
SpeI ACTAGT 2 cut(s) 830, 1303
SsiI CCGC 2 cut(s) 530, 686
SspMI CTAG 3 cut(s) 176, 831, 1304
SstI GAGCTC 1 cut(s) 599
StyD4I CCNGG 4 cut(s) 76, 295, 563, 619
StyI CCWWGG 2 cut(s) 764, 995
TaaI ACNGT 2 cut(s) 224, 252
TaiI ACGT 4 cut(s) 27, 504, 1069, 1186
TaqI TCGA 5 cut(s) 318, 576, 630, 660, 1300
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 2 cut(s) 28, 1272
Tru1I TTAA 2 cut(s) 1082, 1308
Tru9I TTAA 2 cut(s) 1082, 1308
TscAI CASTG 3 cut(s) 255, 747, 985
TseFI GTSAC 2 cut(s) 252, 1162
TseI GCWGC 4 cut(s) 207, 471, 961, 964
Tsp45I GTSAC 2 cut(s) 252, 1162
TspDTI ATGAA 4 cut(s) 20, 30, 336, 540
TspRI CASTG 3 cut(s) 255, 747, 985
Van91I CCANNNNNTGG 2 cut(s) 283, 620
VpaK11BI GGWCC 2 cut(s) 747, 1232
VspI ATTAAT 1 cut(s) 1082
XmiI GTMKAC 1 cut(s) 1048
XmnI GAANNNNTTC 2 cut(s) 989, 1203
XspI CTAG 3 cut(s) 176, 831, 1304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.