MD16G1216300.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014022
Physical Location & Seq
Reverse (-)
21130568 .. 21131644
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1216300.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGATCTTGGAAGCAAAAGCTATTTGATCCATGTGTCACAAGCTCTTCCTCCATCAACAACTCCAGGAAGCTCCATTTTTGGAACTCCGATTCACCATCCCGACACAAGCTTCCCCATTATAGCAGTTGCCGGAATAGGAATTGTAGCCACAGCTTTCTTGCTCGTCAGCTACTACATCTTTGTTATCAAATGCTGCCTCAACTGGCACCGTGTCGACATCCTCAGGCGATTCTCATTGTCGCGCAATAGGCGGGACGAAGACCAGCTCATGGTCTACTCCCCCCGTGTAGAGACCCGGGGGCTCGACGAAGCAGTGATCAGATCAATCCCATTGTTGCAATTCAAGAAAGATGGAAATTACATAGGAATGGGAGAGGGAAGCTTTTGCGAGTGTGCGGTTTGCTTGAATGAGTTTCAAGAAGACGAGAAGCTTCGAATCATACCGAATTGCAGCCACGTTTTCCACATTGATTGTATTGATGTTTGGCTTCAAGGCAATGCCAATTGCCCACTTTGTAGAACAAGCATTTCAACCACACCCAGATTTCCTTTTGATCGAACTGTCGCTCCAAGCTCTTCGCCCCAAGATCCAAGTCCGTACGCAGGTAGCCTCAGTGGAGGCGATGAAGACTACGTGGTTATTGAATTGAGCAACGATAACACGATGGATCATGCAATGCTTGGAAGACAAGGAAGATCGAATTCCGGGGAGTTTTCACTATCAGTAAGATCTATTAGTCCTTCACCAAGGACATTGGAACACAGAGTTTTGCCCAAGAAATTTGAGAGAAAATTTCACAACAAGATTACAAGCATGGGGGATGAATGCATTGATATCAGAGGGAAAGATGATCAGTTTTTAATCCAACCAATAAGGAGGTCTTTCTCAATGGACTCATCAGCTGATAGGCAGCTTTATTTGGCAGCTCAAGCGGCAATGCAGCAGCAAAGGCAAGTCAGTGAGGTCAGTCCCACTGAAGGTTGCAGTAGTGGTAGAGTTAGAAGATCTTTCTTTTCTTTTGGCAATGGTAGGGGATCTAGAAATGCAATTCTGCCTGTTTATTTGGAGCCATAA

Protein Analysis

359

Amino Acids

39.98

Weight (kDa)

7.09

Isoelectric Point (pI)

56.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 131 - 174 1.7e-12 Ring finger domain
zf-RING_11 PF17123 131 - 160 9.9e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 131 - 173 6.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 131 - 175 3.8e-06 zinc-RING finger domain
zf-C3HC4 PF00097 132 - 173 4.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 132 - 171 8e-06 RING-type zinc-finger
zf-rbx1 PF12678 140 - 174 1.3e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43420
fragaria_vesca FvH4_4g03440
malus_domestica MD13G1212600.v1.1 MD16G1216300.v1.1
prunus_persica Prupe.1G037600_v2.0.a1
pyrus_communis pycom13g18440 pycom16g18090
rosa_chinensis RchiOBHm_Chr4g0392431
rosa_laevigata RLG00000009807
rosa_multiflora Rmu_sc0001800.1_g000001
rosa_roxburghii Rroxscaffold_5G00337650
rosa_rugosa Rorug03G0338800
rosa_samantha Rh4AG042800 Rh4BG037800 Rh4CG045800 Rh4DG039900
rosa_wichuraiana Rw4G003340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 596
AccB1I GGYRCC 1 cut(s) 207
AccB7I CCANNNNNTGG 1 cut(s) 271
AccI GTMKAC 2 cut(s) 216, 276
AccII CGCG 1 cut(s) 244
AciI CCGC 3 cut(s) 253, 398, 935
AclWI GGATC 5 cut(s) 12, 22, 584, 678, 1045
AcsI RAATTY 3 cut(s) 703, 782, 794
AcuI CTGAAG 1 cut(s) 999
AfaI GTAC 1 cut(s) 602
AfiI CCNNNNNNNGG 3 cut(s) 271, 605, 980
AgsI TTSAA 6 cut(s) 346, 409, 419, 494, 534, 647
AjnI CCWGG 1 cut(s) 64
AloI GAACNNNNNNTCC 2 cut(s) 553, 585
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 5 cut(s) 12, 22, 584, 678, 1045
Ama87I CYCGRG 1 cut(s) 297
ApeKI GCWGC 6 cut(s) 195, 453, 913, 926, 943, 946
ApoI RAATTY 3 cut(s) 703, 782, 794
AspLEI GCGC 1 cut(s) 246
AsuC2I CCSGG 3 cut(s) 298, 299, 709
AsuHPI GGTGA 2 cut(s) 86, 738
AsuII TTCGAA 1 cut(s) 436
AvaI CYCGRG 1 cut(s) 297
AxyI CCTNAGG 1 cut(s) 224
BanI GGYRCC 1 cut(s) 207
BanII GRGCYC 1 cut(s) 306
BbsI GAAGAC 4 cut(s) 267, 429, 636, 694
BbvI GCAGC 6 cut(s) 182, 465, 925, 938, 955, 958
BccI CCATC 4 cut(s) 61, 105, 347, 661
BciT130I CCWGG 1 cut(s) 66
BclI TGATCA 2 cut(s) 318, 853
BcnI CCSGG 3 cut(s) 298, 299, 709
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 1 cut(s) 1041
BfuAI ACCTGC 1 cut(s) 596
BglII AGATCT 2 cut(s) 731, 1007
BisI GCNGC 7 cut(s) 196, 454, 914, 927, 936, 944, 947
BlsI GCNGC 7 cut(s) 197, 455, 915, 928, 937, 945, 948
Bme1390I CCNGG 4 cut(s) 66, 298, 299, 709
BmeT110I CYCGRG 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 209, 1071
BmrFI CCNGG 4 cut(s) 66, 298, 299, 709
BpiI GAAGAC 4 cut(s) 267, 429, 636, 694
BpmI CTGGAG 1 cut(s) 48
Bpu14I TTCGAA 1 cut(s) 436
BpuEI CTTGAG 1 cut(s) 915
BpuMI CCSGG 3 cut(s) 298, 299, 709
BsaAI YACGTR 1 cut(s) 637
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 4 cut(s) 297, 298, 708, 749
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 605, 980
Bse1I ACTGG 1 cut(s) 209
Bse21I CCTNAGG 1 cut(s) 224
Bse3DI GCAATG 4 cut(s) 505, 684, 945, 1033
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 4 cut(s) 297, 298, 708, 749
BseGI GGATG 3 cut(s) 97, 219, 829
BseLI CCNNNNNNNGG 3 cut(s) 271, 605, 980
BseMI GCAATG 4 cut(s) 505, 684, 945, 1033
BseMII CTCAG 2 cut(s) 238, 628
BseNI ACTGG 1 cut(s) 209
BseXI GCAGC 6 cut(s) 182, 465, 925, 938, 955, 958
Bsh1236I CGCG 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 3 cut(s) 132, 298, 708
BsiWI CGTACG 1 cut(s) 600
BslFI GGGAC 2 cut(s) 269, 957
BslI CCNNNNNNNGG 3 cut(s) 271, 605, 980
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 2 cut(s) 269, 957
BsmI GAATGC 1 cut(s) 833
Bso31I GGTCTC 1 cut(s) 287
BsoBI CYCGRG 1 cut(s) 297
Bsp119I TTCGAA 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 306
BspACI CCGC 3 cut(s) 253, 398, 935
BspCNI CTCAG 2 cut(s) 237, 627
BspFNI CGCG 1 cut(s) 244
BspLI GGNNCC 2 cut(s) 209, 1071
BspMI ACCTGC 1 cut(s) 596
BspPI GGATC 5 cut(s) 12, 22, 584, 678, 1045
BspQI GCTCTTC 2 cut(s) 51, 583
BspT104I TTCGAA 1 cut(s) 436
BspT107I GGYRCC 1 cut(s) 207
BspTNI GGTCTC 1 cut(s) 287
BsrDI GCAATG 4 cut(s) 505, 684, 945, 1033
BsrI ACTGG 1 cut(s) 209
BssECI CCNNGG 4 cut(s) 297, 298, 708, 749
BssT1I CCWWGG 1 cut(s) 749
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 2 cut(s) 212, 565
Bst6I CTCTTC 2 cut(s) 51, 583
BstBAI YACGTR 1 cut(s) 637
BstBI TTCGAA 1 cut(s) 436
BstDEI CTNAG 2 cut(s) 224, 614
BstF5I GGATG 3 cut(s) 97, 219, 829
BstFNI CGCG 1 cut(s) 244
BstHHI GCGC 1 cut(s) 246
BstMAI GTCTC 1 cut(s) 287
BstMWI GCNNNNNNNGC 4 cut(s) 250, 932, 935, 952
BstNI CCWGG 1 cut(s) 66
BstSCI CCNGG 4 cut(s) 64, 296, 297, 707
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 6 cut(s) 182, 465, 925, 938, 955, 958
BstV2I GAAGAC 4 cut(s) 267, 429, 636, 694
BstX2I RGATCY 5 cut(s) 4, 589, 731, 1007, 1037
BstYI RGATCY 5 cut(s) 4, 589, 731, 1007, 1037
Bsu36I CCTNAGG 1 cut(s) 224
BtgZI GCGATG 1 cut(s) 639
BtsCI GGATG 3 cut(s) 97, 219, 829
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 4 cut(s) 321, 622, 967, 975
BveI ACCTGC 1 cut(s) 596
CfoI GCGC 1 cut(s) 246
Cfr9I CCCGGG 1 cut(s) 297
Csp6I GTAC 1 cut(s) 601
CspCI CAANNNNNGTGG 2 cut(s) 455, 490
CviAII CATG 4 cut(s) 32, 271, 674, 817
CviQI GTAC 1 cut(s) 601
DdeI CTNAG 2 cut(s) 224, 614
Eam1104I CTCTTC 2 cut(s) 51, 583
EarI CTCTTC 2 cut(s) 51, 583
Eco130I CCWWGG 1 cut(s) 749
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 287
Eco32I GATATC 1 cut(s) 838
Eco57I CTGAAG 1 cut(s) 999
Eco81I CCTNAGG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 703
EcoRII CCWGG 1 cut(s) 64
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 749
EcoT22I ATGCAT 1 cut(s) 833
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 749
FaeI CATG 4 cut(s) 35, 274, 677, 820
FaiI YATR 8 cut(s) 33, 122, 272, 365, 443, 675, 818, 1075
FalI AAGNNNNNCTT 2 cut(s) 868, 900
FaqI GGGAC 2 cut(s) 269, 957
FatI CATG 4 cut(s) 31, 270, 673, 816
FauI CCCGC 1 cut(s) 246
FbaI TGATCA 2 cut(s) 318, 853
FblI GTMKAC 2 cut(s) 216, 276
Fnu4HI GCNGC 7 cut(s) 196, 454, 914, 927, 936, 944, 947
FokI GGATG 3 cut(s) 84, 206, 836
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 7 cut(s) 196, 454, 914, 927, 936, 944, 947
FspBI CTAG 1 cut(s) 1041
GlaI GCGC 1 cut(s) 245
GluI GCNGC 7 cut(s) 196, 454, 914, 927, 936, 944, 947
GsuI CTGGAG 1 cut(s) 48
HapII CCGG 3 cut(s) 132, 298, 708
HhaI GCGC 1 cut(s) 246
Hin1II CATG 4 cut(s) 35, 274, 677, 820
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 3 cut(s) 109, 382, 431
HinfI GANTC 4 cut(s) 91, 231, 438, 896
HpaII CCGG 3 cut(s) 132, 298, 708
HphI GGTGA 2 cut(s) 86, 738
Hpy166II GTNNAC 2 cut(s) 217, 277
Hpy188I TCNGA 3 cut(s) 90, 323, 842
Hpy188III TCNNGA 4 cut(s) 101, 346, 419, 1041
Hpy8I GTNNAC 2 cut(s) 217, 277
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 2 cut(s) 753, 974
HpyCH4III ACNGT 2 cut(s) 212, 565
HpyCH4IV ACGT 2 cut(s) 459, 636
HpyCH4V TGCA 7 cut(s) 340, 453, 677, 831, 943, 987, 1049
HpyF10VI GCNNNNNNNGC 4 cut(s) 250, 932, 935, 952
HpyF3I CTNAG 2 cut(s) 224, 614
HpySE526I ACGT 2 cut(s) 459, 636
Hsp92II CATG 4 cut(s) 35, 274, 677, 820
HspAI GCGC 1 cut(s) 244
Ksp22I TGATCA 2 cut(s) 318, 853
LguI GCTCTTC 2 cut(s) 51, 583
LmnI GCTCC 3 cut(s) 77, 574, 1069
Lsp1109I GCAGC 6 cut(s) 182, 465, 925, 938, 955, 958
MaeI CTAG 1 cut(s) 1041
MaeII ACGT 2 cut(s) 459, 636
MaeIII GTNAC 1 cut(s) 36
MboII GAAGA 8 cut(s) 38, 272, 434, 570, 641, 699, 708, 1017
MfeI CAATTG 1 cut(s) 505
MflI RGATCY 5 cut(s) 4, 589, 731, 1007, 1037
MhlI GDGCHC 1 cut(s) 306
MlyI GAGTC 1 cut(s) 890
MmeI TCCRAC 1 cut(s) 892
MnlI CCTC 9 cut(s) 60, 209, 233, 370, 614, 623, 836, 873, 958
Mph1103I ATGCAT 1 cut(s) 833
MseI TTAA 1 cut(s) 863
MslI CAYNNNNRTG 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 3 cut(s) 132, 298, 708
MspR9I CCNGG 4 cut(s) 66, 298, 299, 709
MunI CAATTG 1 cut(s) 505
Mva1269I GAATGC 1 cut(s) 833
MvaI CCWGG 1 cut(s) 66
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 4 cut(s) 250, 932, 935, 952
NciI CCSGG 3 cut(s) 298, 299, 709
NlaIII CATG 4 cut(s) 35, 274, 677, 820
NlaIV GGNNCC 2 cut(s) 209, 1071
NmuCI GTSAC 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 833
NspV TTCGAA 1 cut(s) 436
PciSI GCTCTTC 2 cut(s) 51, 583
PctI GAATGC 1 cut(s) 833
PfeI GAWTC 3 cut(s) 91, 231, 438
Pfl23II CGTACG 1 cut(s) 600
PflMI CCANNNNNTGG 1 cut(s) 271
PfoI TCCNGGA 1 cut(s) 64
PkrI GCNGC 7 cut(s) 197, 455, 915, 928, 937, 945, 948
PleI GAGTC 1 cut(s) 890
PpsI GAGTC 1 cut(s) 890
Ppu21I YACGTR 1 cut(s) 637
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PspLI CGTACG 1 cut(s) 600
PspN4I GGNNCC 2 cut(s) 209, 1071
PsuI RGATCY 5 cut(s) 4, 589, 731, 1007, 1037
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 1 cut(s) 602
RsaNI GTAC 1 cut(s) 601
RseI CAYNNNNRTG 1 cut(s) 368
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 2 cut(s) 51, 583
SaqAI TTAA 1 cut(s) 863
SatI GCNGC 7 cut(s) 196, 454, 914, 927, 936, 944, 947
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 4 cut(s) 66, 298, 299, 709
SduI GDGCHC 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 436
SmaI CCCGGG 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 930
SmoI CTYRAG 1 cut(s) 930
SsiI CCGC 3 cut(s) 253, 398, 935
SspMI CTAG 1 cut(s) 1041
StyD4I CCNGG 4 cut(s) 64, 296, 297, 707
StyI CCWWGG 1 cut(s) 749
TaaI ACNGT 2 cut(s) 212, 565
TaiI ACGT 2 cut(s) 462, 639
TaqI TCGA 5 cut(s) 216, 306, 436, 559, 701
TauI GCSGC 1 cut(s) 938
TfiI GAWTC 3 cut(s) 91, 231, 438
Tru1I TTAA 1 cut(s) 863
Tru9I TTAA 1 cut(s) 863
TscAI CASTG 4 cut(s) 321, 622, 967, 982
TseFI GTSAC 1 cut(s) 36
TseI GCWGC 6 cut(s) 195, 453, 913, 926, 943, 946
Tsp45I GTSAC 1 cut(s) 36
TspDTI ATGAA 2 cut(s) 642, 840
TspGWI ACGGA 1 cut(s) 588
TspMI CCCGGG 1 cut(s) 297
TspRI CASTG 4 cut(s) 321, 622, 967, 982
Van91I CCANNNNNTGG 1 cut(s) 271
XapI RAATTY 3 cut(s) 703, 782, 794
XbaI TCTAGA 1 cut(s) 1040
XmaI CCCGGG 1 cut(s) 297
XmiI GTMKAC 2 cut(s) 216, 276
XspI CTAG 1 cut(s) 1041
Zsp2I ATGCAT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.