Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014022

Physical Location & Seq

Genomic Coordinates Reverse (-)

19844177 .. 19845259

Gene Structure 1083 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1212600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1083 bp

ATGGATCTTGGAAGCAAAAGCTATTTGATCCACGTGTCCCAAGCTCTTCCACCAACAACTTCAGGAAGCTCCATTTTTGGAACTCCGATTCACCATTCCGACACAAGCTTCCCAATTATAGCGGTTGCCATAATAGGAATTATAGCCACAGCTTTCTTGCTCGTCAGCTACTACATCTTTGTTATCAAATGCTGCCTCAACTGGCACCGAGTCGATATCCTCGGACGATTCTCATTGTCGCGTAATACGCGGGACGAAGACCAGCTAATGGTGTACTCCCCTGGGATAGAGACCCGGGGGCTTGACGAGGCGGTGATCAGATCAATCCCATTGTTGCAATTCAAGAAAGACGGAAATTACAGAGGACTTGGAGAGGGAAGCTTTTACGAATGTGCGGTTTGCTTGAATGAGTTTCATGAAGACGAGAAGCTTCGCATCATACCAAACTGCAGCCACGTTTTCCACATTGATTGCATTGATGTTTGGCTTCAAAGCAATGCCAATTGCCCACTTTGTAGAACAAGCATTTCAACTGCACCCCGATATCCTTTCGGTCGAACTGTCGCTCCAAGCTCTTCTCCCCGAGATCCAAGTCCATACGCAGGTAGCCTCAGTGGCGGTGATGAAGACTACGTGGTGATCGAATTGAGCAACGATAACTCGATGGATCAACCAATGCTTGGAAGACAAGAAATATGGAATTCCGGGGACTTATCACTATTAGTGAGGTCTATTAGTCCTTCACCAAGGAAATTGGAACATAAAGTTTTGCCCAAGAAATTTGGAAGAAAGTTTCACAACAAGGTTACAAGCATGGGGGATGAATGCATTGAAATTAGAGGGAAAGATGAGCAGTTTTCAATCCAACCAATAAGGAGGTCTTTCTCAATGGACTCATCGGCTGATAGGCAGCTTTATTTGGCAGTTCAAGAGGCAATGCAGCAGCAAAGGCAAGTCAATGAGGTCAGTCCCATTGAAGGTTGCAGTAGTACTGGTAGTGATAGAGTTGGAAGATCTTTCTTTTCTTTTGGCAATGGTAGGGGAACCCGAAATGCAATTCTGCCTGTTTATTTGGAGCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

361

Amino Acids

40.15

Weight (kDa)

6.32

Isoelectric Point (pI)

54.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	130 - 173	1e-13	Ring finger domain
zf-RING_11	PF17123	130 - 159	1.4e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	130 - 172	7.4e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	130 - 174	4e-06	zinc-RING finger domain
zf-C3HC4	PF00097	131 - 172	2.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	140 - 173	1e-08	RING-H2 zinc finger domain
zf-ANAPC11	PF12861	141 - 178	1e-05	Anaphase-promoting complex subunit 11 RING-H2 finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014022)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43420
fragaria_vesca	FvH4_4g03440
malus_domestica	MD13G1212600.v1.1 MD16G1216300.v1.1
prunus_persica	Prupe.1G037600_v2.0.a1
pyrus_communis	pycom13g18440 pycom16g18090
rosa_chinensis	RchiOBHm_Chr4g0392431
rosa_laevigata	RLG00000009807
rosa_multiflora	Rmu_sc0001800.1_g000001
rosa_roxburghii	Rroxscaffold_5G00337650
rosa_rugosa	Rorug03G0338800
rosa_samantha	Rh4AG042800 Rh4BG037800 Rh4CG045800 Rh4DG039900
rosa_wichuraiana	Rw4G003340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	593
AccB1I	GGYRCC	1 cut(s)	204
AccB7I	CCANNNNNTGG	2 cut(s)	268, 680
AccII	CGCG	2 cut(s)	241, 250
AciI	CCGC	5 cut(s)	122, 250, 311, 395, 618
AclWI	GGATC	4 cut(s)	12, 22, 581, 675
AcsI	RAATTY	2 cut(s)	700, 779
AcuI	CTGAAG	1 cut(s)	45
AcvI	CACGTG	1 cut(s)	34
AfaI	GTAC	2 cut(s)	275, 991
AfiI	CCNNNNNNNGG	4 cut(s)	268, 602, 680, 977
AflIII	ACRYGT	1 cut(s)	33
AgsI	TTSAA	8 cut(s)	343, 406, 491, 531, 833, 861, 929, 977
AjnI	CCWGG	1 cut(s)	280
AloI	GAACNNNNNNTCC	2 cut(s)	550, 582
Alw26I	GTCTC	1 cut(s)	284
AlwI	GGATC	4 cut(s)	12, 22, 581, 675
Ama87I	CYCGRG	2 cut(s)	294, 582
ApeKI	GCWGC	5 cut(s)	192, 450, 910, 940, 943
ApoI	RAATTY	2 cut(s)	700, 779
AsuC2I	CCSGG	3 cut(s)	295, 296, 706
AsuHPI	GGTGA	5 cut(s)	83, 325, 632, 649, 735
AvaI	CYCGRG	2 cut(s)	294, 582
BanI	GGYRCC	1 cut(s)	204
BbrPI	CACGTG	1 cut(s)	34
BbsI	GAAGAC	4 cut(s)	264, 426, 633, 691
BbvI	GCAGC	5 cut(s)	179, 462, 922, 952, 955
BccI	CCATC	1 cut(s)	658
BciT130I	CCWGG	1 cut(s)	282
BclI	TGATCA	1 cut(s)	315
BcnI	CCSGG	3 cut(s)	295, 296, 706
BcoDI	GTCTC	1 cut(s)	284
BfmI	CTRYAG	1 cut(s)	448
BfuAI	ACCTGC	1 cut(s)	593
BglI	GCCNNNNNGGC	1 cut(s)	615
BglII	AGATCT	1 cut(s)	1013
BisI	GCNGC	5 cut(s)	193, 451, 911, 941, 944
BlsI	GCNGC	5 cut(s)	194, 452, 912, 942, 945
BmcAI	AGTACT	1 cut(s)	991
Bme1390I	CCNGG	4 cut(s)	282, 295, 296, 706
BmeT110I	CYCGRG	2 cut(s)	294, 582
BmiI	GGNNCC	3 cut(s)	206, 1045, 1077
BmrFI	CCNGG	4 cut(s)	282, 295, 296, 706
BmsI	GCATC	1 cut(s)	444
BpiI	GAAGAC	4 cut(s)	264, 426, 633, 691
BpuMI	CCSGG	3 cut(s)	295, 296, 706
BsaAI	YACGTR	2 cut(s)	34, 634
BsaI	GGTCTC	1 cut(s)	284
BsaJI	CCNNGG	7 cut(s)	220, 280, 281, 294, 295, 705, 746
BsaXI	ACNNNNNCTCC	2 cut(s)	550, 580
Bsc4I	CCNNNNNNNGG	4 cut(s)	268, 602, 680, 977
Bse1I	ACTGG	2 cut(s)	206, 997
Bse3DI	GCAATG	3 cut(s)	502, 942, 1039
BseBI	CCWGG	1 cut(s)	282
BseDI	CCNNGG	7 cut(s)	220, 280, 281, 294, 295, 705, 746
BseGI	GGATG	1 cut(s)	826
BseLI	CCNNNNNNNGG	4 cut(s)	268, 602, 680, 977
BseMI	GCAATG	3 cut(s)	502, 942, 1039
BseMII	CTCAG	1 cut(s)	625
BseNI	ACTGG	2 cut(s)	206, 997
BseXI	GCAGC	5 cut(s)	179, 462, 922, 952, 955
BsgI	GTGCAG	1 cut(s)	519
Bsh1236I	CGCG	2 cut(s)	241, 250
Bsh1285I	CGRYCG	1 cut(s)	556
BshNI	GGYRCC	1 cut(s)	204
BsiEI	CGRYCG	1 cut(s)	556
BsiHKCI	CYCGRG	2 cut(s)	294, 582
BsiSI	CCGG	2 cut(s)	295, 705
BslFI	GGGAC	4 cut(s)	22, 266, 722, 954
BslI	CCNNNNNNNGG	4 cut(s)	268, 602, 680, 977
BsmAI	GTCTC	1 cut(s)	284
BsmFI	GGGAC	4 cut(s)	22, 266, 722, 954
BsmI	GAATGC	1 cut(s)	830
Bso31I	GGTCTC	1 cut(s)	284
BsoBI	CYCGRG	2 cut(s)	294, 582
Bsp143I	GATC	8 cut(s)	4, 27, 315, 320, 586, 639, 667, 1013
BspACI	CCGC	5 cut(s)	122, 250, 311, 395, 618
BspCNI	CTCAG	1 cut(s)	624
BspFNI	CGCG	2 cut(s)	241, 250
BspHI	TCATGA	1 cut(s)	415
BspLI	GGNNCC	3 cut(s)	206, 1045, 1077
BspMAI	CTGCAG	1 cut(s)	452
BspMI	ACCTGC	1 cut(s)	593
BspPI	GGATC	4 cut(s)	12, 22, 581, 675
BspQI	GCTCTTC	2 cut(s)	51, 580
BspT107I	GGYRCC	1 cut(s)	204
BspTNI	GGTCTC	1 cut(s)	284
BsrDI	GCAATG	3 cut(s)	502, 942, 1039
BsrI	ACTGG	2 cut(s)	206, 997
BssECI	CCNNGG	7 cut(s)	220, 280, 281, 294, 295, 705, 746
BssMI	GATC	8 cut(s)	4, 27, 315, 320, 586, 639, 667, 1013
BssT1I	CCWWGG	1 cut(s)	746
Bst2UI	CCWGG	1 cut(s)	282
Bst4CI	ACNGT	1 cut(s)	562
Bst6I	CTCTTC	2 cut(s)	51, 580
BstBAI	YACGTR	2 cut(s)	34, 634
BstDEI	CTNAG	1 cut(s)	611
BstF5I	GGATG	1 cut(s)	826
BstFNI	CGCG	2 cut(s)	241, 250
BstKTI	GATC	8 cut(s)	7, 30, 318, 323, 589, 642, 670, 1016
BstMAI	GTCTC	1 cut(s)	284
BstMBI	GATC	8 cut(s)	4, 27, 315, 320, 586, 639, 667, 1013
BstMCI	CGRYCG	1 cut(s)	556
BstMWI	GCNNNNNNNGC	3 cut(s)	247, 615, 949
BstNI	CCWGG	1 cut(s)	282
BstSCI	CCNGG	4 cut(s)	280, 293, 294, 704
BstSFI	CTRYAG	1 cut(s)	448
BstUI	CGCG	2 cut(s)	241, 250
BstV1I	GCAGC	5 cut(s)	179, 462, 922, 952, 955
BstV2I	GAAGAC	4 cut(s)	264, 426, 633, 691
BstX2I	RGATCY	3 cut(s)	4, 586, 1013
BstYI	RGATCY	3 cut(s)	4, 586, 1013
BtsCI	GGATG	1 cut(s)	826
BtsIMutI	CAGTG	1 cut(s)	619
BveI	ACCTGC	1 cut(s)	593
CciI	TCATGA	1 cut(s)	415
Cfr9I	CCCGGG	1 cut(s)	294
Csp6I	GTAC	2 cut(s)	274, 990
CspCI	CAANNNNNGTGG	2 cut(s)	452, 487
CviAII	CATG	2 cut(s)	416, 814
CviQI	GTAC	2 cut(s)	274, 990
DdeI	CTNAG	1 cut(s)	611
DpnI	GATC	8 cut(s)	6, 29, 317, 322, 588, 641, 669, 1015
DpnII	GATC	8 cut(s)	4, 27, 315, 320, 586, 639, 667, 1013
Eam1104I	CTCTTC	2 cut(s)	51, 580
EarI	CTCTTC	2 cut(s)	51, 580
Eco130I	CCWWGG	1 cut(s)	746
Eco31I	GGTCTC	1 cut(s)	284
Eco32I	GATATC	2 cut(s)	217, 545
Eco57I	CTGAAG	1 cut(s)	45
Eco72I	CACGTG	1 cut(s)	34
Eco88I	CYCGRG	2 cut(s)	294, 582
EcoRI	GAATTC	1 cut(s)	700
EcoRII	CCWGG	1 cut(s)	280
EcoRV	GATATC	2 cut(s)	217, 545
EcoT14I	CCWWGG	1 cut(s)	746
EcoT22I	ATGCAT	1 cut(s)	830
ErhI	CCWWGG	1 cut(s)	746
FaeI	CATG	2 cut(s)	419, 817
FalI	AAGNNNNNCTT	2 cut(s)	865, 897
FaqI	GGGAC	4 cut(s)	22, 266, 722, 954
FatI	CATG	2 cut(s)	415, 813
FauI	CCCGC	1 cut(s)	243
FbaI	TGATCA	1 cut(s)	315
Fnu4HI	GCNGC	5 cut(s)	193, 451, 911, 941, 944
FokI	GGATG	1 cut(s)	833
Fsp4HI	GCNGC	5 cut(s)	193, 451, 911, 941, 944
GluI	GCNGC	5 cut(s)	193, 451, 911, 941, 944
HapII	CCGG	2 cut(s)	295, 705
Hin1II	CATG	2 cut(s)	419, 817
HindIII	AAGCTT	3 cut(s)	106, 379, 428
HinfI	GANTC	4 cut(s)	88, 210, 228, 893
HpaII	CCGG	2 cut(s)	295, 705
HphI	GGTGA	5 cut(s)	83, 325, 632, 649, 735
Hpy166II	GTNNAC	1 cut(s)	274
Hpy188I	TCNGA	4 cut(s)	87, 100, 224, 320
Hpy188III	TCNNGA	4 cut(s)	63, 343, 416, 929
Hpy8I	GTNNAC	1 cut(s)	274
HpyAV	CCTTC	2 cut(s)	750, 971
HpyCH4III	ACNGT	1 cut(s)	562
HpyCH4IV	ACGT	3 cut(s)	33, 456, 633
HpyCH4V	TGCA	8 cut(s)	337, 450, 474, 536, 828, 940, 984, 1055
HpyF10VI	GCNNNNNNNGC	3 cut(s)	247, 615, 949
HpyF3I	CTNAG	1 cut(s)	611
HpySE526I	ACGT	3 cut(s)	33, 456, 633
Hsp92II	CATG	2 cut(s)	419, 817
Ksp22I	TGATCA	1 cut(s)	315
Kzo9I	GATC	8 cut(s)	4, 27, 315, 320, 586, 639, 667, 1013
LguI	GCTCTTC	2 cut(s)	51, 580
LmnI	GCTCC	3 cut(s)	74, 571, 1075
Lsp1109I	GCAGC	5 cut(s)	179, 462, 922, 952, 955
LweI	GCATC	1 cut(s)	444
MaeII	ACGT	3 cut(s)	33, 456, 633
MaeIII	GTNAC	1 cut(s)	805
MalI	GATC	8 cut(s)	6, 29, 317, 322, 588, 641, 669, 1015
MboI	GATC	8 cut(s)	4, 27, 315, 320, 586, 639, 667, 1013
MboII	GAAGA	8 cut(s)	38, 269, 431, 567, 638, 696, 798, 1023
MfeI	CAATTG	1 cut(s)	502
MflI	RGATCY	3 cut(s)	4, 586, 1013
MlyI	GAGTC	2 cut(s)	219, 887
MmeI	TCCRAC	3 cut(s)	123, 889, 988
Mph1103I	ATGCAT	1 cut(s)	830
MspI	CCGG	2 cut(s)	295, 705
MspR9I	CCNGG	4 cut(s)	282, 295, 296, 706
MunI	CAATTG	1 cut(s)	502
Mva1269I	GAATGC	1 cut(s)	830
MvaI	CCWGG	1 cut(s)	282
MvnI	CGCG	2 cut(s)	241, 250
MwoI	GCNNNNNNNGC	3 cut(s)	247, 615, 949
NciI	CCSGG	3 cut(s)	295, 296, 706
NdeII	GATC	8 cut(s)	4, 27, 315, 320, 586, 639, 667, 1013
NlaIII	CATG	2 cut(s)	419, 817
NlaIV	GGNNCC	3 cut(s)	206, 1045, 1077
NsiI	ATGCAT	1 cut(s)	830
PagI	TCATGA	1 cut(s)	415
PasI	CCCWGGG	1 cut(s)	281
PciSI	GCTCTTC	2 cut(s)	51, 580
PcsI	WCGNNNNNNNCGW	1 cut(s)	639
PctI	GAATGC	1 cut(s)	830
PfeI	GAWTC	2 cut(s)	88, 228
PflMI	CCANNNNNTGG	2 cut(s)	268, 680
PkrI	GCNGC	5 cut(s)	194, 452, 912, 942, 945
PleI	GAGTC	2 cut(s)	218, 887
PmaCI	CACGTG	1 cut(s)	34
PmlI	CACGTG	1 cut(s)	34
PpsI	GAGTC	2 cut(s)	218, 887
Ppu21I	YACGTR	2 cut(s)	34, 634
Psp6I	CCWGG	1 cut(s)	280
PspCI	CACGTG	1 cut(s)	34
PspGI	CCWGG	1 cut(s)	280
PspN4I	GGNNCC	3 cut(s)	206, 1045, 1077
PstI	CTGCAG	1 cut(s)	452
PsuI	RGATCY	3 cut(s)	4, 586, 1013
RsaI	GTAC	2 cut(s)	275, 991
RsaNI	GTAC	2 cut(s)	274, 990
SapI	GCTCTTC	2 cut(s)	51, 580
SatI	GCNGC	5 cut(s)	193, 451, 911, 941, 944
Sau3AI	GATC	8 cut(s)	4, 27, 315, 320, 586, 639, 667, 1013
ScaI	AGTACT	1 cut(s)	991
SchI	GAGTC	2 cut(s)	219, 887
ScrFI	CCNGG	4 cut(s)	282, 295, 296, 706
SfaNI	GCATC	1 cut(s)	444
SfcI	CTRYAG	1 cut(s)	448
SmaI	CCCGGG	1 cut(s)	296
SsiI	CCGC	5 cut(s)	122, 250, 311, 395, 618
StyD4I	CCNGG	4 cut(s)	280, 293, 294, 704
StyI	CCWWGG	1 cut(s)	746
TaaI	ACNGT	1 cut(s)	562
TaiI	ACGT	3 cut(s)	36, 459, 636
TaqI	TCGA	4 cut(s)	213, 556, 642, 662
TaqII	GACCGA	1 cut(s)	542
TatI	WGTACW	2 cut(s)	273, 989
TfiI	GAWTC	2 cut(s)	88, 228
TscAI	CASTG	1 cut(s)	619
TseI	GCWGC	5 cut(s)	192, 450, 910, 940, 943
TspDTI	ATGAA	4 cut(s)	404, 432, 639, 837
TspGWI	ACGGA	1 cut(s)	366
TspMI	CCCGGG	1 cut(s)	294
TspRI	CASTG	1 cut(s)	619
Van91I	CCANNNNNTGG	2 cut(s)	268, 680
XapI	RAATTY	2 cut(s)	700, 779
XmaI	CCCGGG	1 cut(s)	294
ZrmI	AGTACT	1 cut(s)	991
Zsp2I	ATGCAT	1 cut(s)	830

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.