Rh4DG039900

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014022
Physical Location & Seq
Reverse (-)
6444077 .. 6458449
14373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG039900.1

Sequence Viewer

Length: 1077 bp
ATGGATCTTGTAAGAAAAGGCTACTTGATTCATGTCTCTTCATCAGAAGCACAAGCATTTGCTCCAATTACAAGTCCAGGAAGCTCAGTCTTTGGGACTCCTATTCATCATTCAGACACTAGCTTCCCCATTATAGCAATTGCCATAATAGGAATTATAGCCACGGCTTTCTTGCTAGTGAGCTACTACATCTTTGTCATCAAGTGCTGCCTCAACTGGCACCGTGTGGATCTCCTCAGGCGATTTTCACTGTCACGAAACCGGCGCGACGAAGACCAGCTAATGGTGTACTCGCCGGGTTTGGAGACCCGAGGGCTCGATGAAGCTGTGATCAGATCAATCCCATTGTTGCAGTTCAAGAAAGAAGGGAAGAACAGAGGTGGGTTTGAACAAGGAAGCTTGTTTTGTGAGTGTGCAGTTTGCTTGAATGAGTTTCAAGAAGATGAGAAGCTGAGGATCATACCCAACTGCAGCCATGTTTTCCACATTGACTGCATTGACGTTTGGCTTCAAAACAACGCGAATTGCCCGCTTTGTAGAACAAGCATTTCATCCAACACAACCCGGTTCCAATTCGATCAAATCGTGGCTCCGAGCTCTTCACCTCAAAATCCAACCCCTGGGAGTCTCAATGGAGGCGACGACGACTACGTGGTGATCGAATTGAGCAACGAAAACAATCCGGCGGATCAAGCGTTGATGCTTGGAGTGCAAGAAAAATTGAACTCAGGGGAGTTGTCAGTGAGGTCCATTAGTCCTTCCCCAAGGAAATTGGAACAAATTAGAACATTTGTGCCCAAAAAGGCAAGAAAGTTTCACAACAAGGTTACTAGTATGGGTGATGAGTGCATTGACATTAGGGGCAAAGATGAGCAATTCTCCATCCAACCCATAAGGAGGTCTTTCTCTATGGACTCATCTAATGATAGACAGCTCTATTTGGCAGTTCAAGAAGCTGTGCAGCAGCAAAGCCAAGTCAGTGAGGTCAGTCCAATTGAAGGTTGCAGTAGCAGTACTAGTTCTAGAGTCAGAAGATGTTCAGAATCAATTGCCAACTACCAGTTGATGATCGACTAA

Protein Analysis

358

Amino Acids

40.06

Weight (kDa)

6.02

Isoelectric Point (pI)

56.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 137 - 180 6.8e-13 Ring finger domain
zf-RING_11 PF17123 137 - 166 9.9e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 137 - 179 3.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 137 - 181 3.2e-06 zinc-RING finger domain
zf-C3HC4 PF00097 138 - 179 4.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 146 - 180 9.9e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43420
fragaria_vesca FvH4_4g03440
malus_domestica MD13G1212600.v1.1 MD16G1216300.v1.1
prunus_persica Prupe.1G037600_v2.0.a1
pyrus_communis pycom13g18440 pycom16g18090
rosa_chinensis RchiOBHm_Chr4g0392431
rosa_laevigata RLG00000009807
rosa_multiflora Rmu_sc0001800.1_g000001
rosa_roxburghii Rroxscaffold_5G00337650
rosa_rugosa Rorug03G0338800
rosa_samantha Rh4AG042800 Rh4BG037800 Rh4CG045800 Rh4DG039900
rosa_wichuraiana Rw4G003340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 219
AccB7I CCANNNNNTGG 2 cut(s) 283, 620
AccII CGCG 2 cut(s) 267, 521
AciI CCGC 2 cut(s) 530, 686
AclWI GGATC 4 cut(s) 12, 237, 464, 696
AdeI CACNNNGTG 1 cut(s) 226
AfaI GTAC 2 cut(s) 290, 1015
AfiI CCNNNNNNNGG 4 cut(s) 283, 620, 897, 998
AgsI TTSAA 8 cut(s) 358, 389, 427, 437, 512, 724, 950, 998
AhlI ACTAGT 2 cut(s) 830, 1016
AjnI CCWGG 2 cut(s) 76, 619
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 3 cut(s) 40, 299, 632
AlwI GGATC 4 cut(s) 12, 237, 464, 696
Ama87I CYCGRG 1 cut(s) 309
ApeKI GCWGC 4 cut(s) 207, 471, 961, 964
Asp700I GAANNNNTTC 1 cut(s) 1036
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 747
AsuC2I CCSGG 2 cut(s) 297, 565
AsuHPI GGTGA 3 cut(s) 594, 667, 851
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 1 cut(s) 747
AxyI CCTNAGG 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 798
BanI GGYRCC 1 cut(s) 219
BanII GRGCYC 2 cut(s) 318, 599
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 599
BbvCI CCTCAGC 1 cut(s) 452
BbvI GCAGC 4 cut(s) 194, 483, 973, 976
BccI CCATC 1 cut(s) 890
BceAI ACGGC 1 cut(s) 180
BciT130I CCWGG 2 cut(s) 78, 621
BclI TGATCA 1 cut(s) 330
BcnI CCSGG 2 cut(s) 297, 565
BcoDI GTCTC 3 cut(s) 40, 299, 632
BcuI ACTAGT 2 cut(s) 830, 1016
BfaI CTAG 5 cut(s) 120, 176, 831, 1017, 1023
BfmI CTRYAG 1 cut(s) 469
BisI GCNGC 4 cut(s) 208, 472, 962, 965
BlsI GCNGC 4 cut(s) 209, 473, 963, 966
BmcAI AGTACT 1 cut(s) 1015
Bme1390I CCNGG 4 cut(s) 78, 297, 565, 621
Bme18I GGWCC 1 cut(s) 747
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 747
BmiI GGNNCC 3 cut(s) 221, 569, 591
BmrFI CCNGG 4 cut(s) 78, 297, 565, 621
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 279
BplI GAGNNNNNCTC 2 cut(s) 863, 895
Bpu10I CCTNAGC 1 cut(s) 452
BpuMI CCSGG 2 cut(s) 297, 565
BsaAI YACGTR 1 cut(s) 652
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 5 cut(s) 162, 310, 619, 620, 764
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 620, 897, 998
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 2 cut(s) 221, 1060
Bse21I CCTNAGG 1 cut(s) 236
BseBI CCWGG 2 cut(s) 78, 621
BseDI CCNNGG 5 cut(s) 162, 310, 619, 620, 764
BseGI GGATG 2 cut(s) 551, 882
BseLI CCNNNNNNNGG 4 cut(s) 283, 620, 897, 998
BseMII CTCAG 4 cut(s) 99, 250, 443, 741
BseNI ACTGG 2 cut(s) 221, 1060
BseRI GAGGAG 1 cut(s) 224
BseSI GKGCMC 1 cut(s) 798
BseXI GCAGC 4 cut(s) 194, 483, 973, 976
BsgI GTGCAG 2 cut(s) 435, 980
Bsh1236I CGCG 2 cut(s) 267, 521
BshNI GGYRCC 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 599
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 4 cut(s) 262, 296, 565, 683
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 4 cut(s) 283, 620, 897, 998
BsmAI GTCTC 3 cut(s) 40, 299, 632
BsmFI GGGAC 1 cut(s) 109
Bso31I GGTCTC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 3 cut(s) 318, 599, 798
Bsp143I GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1068
BspACI CCGC 2 cut(s) 530, 686
BspCNI CTCAG 4 cut(s) 98, 249, 444, 740
BspFNI CGCG 2 cut(s) 267, 521
BspLI GGNNCC 3 cut(s) 221, 569, 591
BspMAI CTGCAG 1 cut(s) 473
BspPI GGATC 4 cut(s) 12, 237, 464, 696
BspQI GCTCTTC 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 219
BspTNI GGTCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 2 cut(s) 221, 1060
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 5 cut(s) 162, 310, 619, 620, 764
BssMI GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1068
BssT1I CCWWGG 1 cut(s) 764
Bst2UI CCWGG 2 cut(s) 78, 621
Bst4CI ACNGT 2 cut(s) 224, 252
Bst6I CTCTTC 2 cut(s) 43, 604
BstBAI YACGTR 1 cut(s) 652
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 4 cut(s) 85, 236, 452, 727
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 2 cut(s) 551, 882
BstFNI CGCG 2 cut(s) 267, 521
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 9 cut(s) 7, 232, 333, 338, 459, 580, 660, 691, 1071
BstMAI GTCTC 3 cut(s) 40, 299, 632
BstMBI GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1068
BstMWI GCNNNNNNNGC 2 cut(s) 692, 709
BstNI CCWGG 2 cut(s) 78, 621
BstSCI CCNGG 4 cut(s) 76, 295, 563, 619
BstSFI CTRYAG 1 cut(s) 469
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 2 cut(s) 267, 521
BstV1I GCAGC 4 cut(s) 194, 483, 973, 976
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 2 cut(s) 4, 229
BstYI RGATCY 2 cut(s) 4, 229
Bsu36I CCTNAGG 1 cut(s) 236
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 2 cut(s) 551, 882
BtsIMutI CAGTG 3 cut(s) 248, 747, 985
Cac8I GCNNGC 1 cut(s) 530
CfoI GCGC 1 cut(s) 267
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 747
Csp6I GTAC 2 cut(s) 289, 1014
CviAII CATG 2 cut(s) 32, 476
CviQI GTAC 2 cut(s) 289, 1014
DdeI CTNAG 4 cut(s) 85, 236, 452, 727
DpnI GATC 9 cut(s) 6, 231, 332, 337, 458, 579, 659, 690, 1070
DpnII GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1068
DraIII CACNNNGTG 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 43, 604
EarI CTCTTC 2 cut(s) 43, 604
EciI GGCGGA 1 cut(s) 701
Ecl136II GAGCTC 1 cut(s) 597
Eco130I CCWWGG 1 cut(s) 764
Eco24I GRGCYC 2 cut(s) 318, 599
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 747
Eco53kI GAGCTC 1 cut(s) 597
Eco81I CCTNAGG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 597
EcoRII CCWGG 2 cut(s) 76, 619
EcoT14I CCWWGG 1 cut(s) 764
EcoT38I GRGCYC 2 cut(s) 318, 599
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 2 cut(s) 35, 479
FaiI YATR 9 cut(s) 33, 134, 146, 158, 461, 477, 836, 893, 911
FalI AAGNNNNNCTT 2 cut(s) 886, 918
FaqI GGGAC 1 cut(s) 109
FatI CATG 2 cut(s) 31, 475
FauI CCCGC 1 cut(s) 537
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 4 cut(s) 208, 472, 962, 965
FokI GGATG 2 cut(s) 538, 869
FriOI GRGCYC 2 cut(s) 318, 599
Fsp4HI GCNGC 4 cut(s) 208, 472, 962, 965
FspBI CTAG 5 cut(s) 120, 176, 831, 1017, 1023
GlaI GCGC 1 cut(s) 266
GluI GCNGC 4 cut(s) 208, 472, 962, 965
HapII CCGG 4 cut(s) 262, 296, 565, 683
HhaI GCGC 1 cut(s) 267
Hin1II CATG 2 cut(s) 35, 479
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 397
HinfI GANTC 6 cut(s) 28, 97, 625, 914, 1026, 1043
HpaII CCGG 4 cut(s) 262, 296, 565, 683
HphI GGTGA 3 cut(s) 594, 667, 851
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 6 cut(s) 46, 115, 335, 594, 1031, 1042
Hpy188III TCNNGA 5 cut(s) 255, 358, 437, 950, 1023
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 3 cut(s) 272, 644, 647
HpyAV CCTTC 3 cut(s) 359, 768, 992
HpyCH4III ACNGT 2 cut(s) 224, 252
HpyCH4IV ACGT 2 cut(s) 501, 651
HpyCH4V TGCA 8 cut(s) 352, 416, 471, 495, 712, 849, 961, 1005
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 709
HpyF3I CTNAG 4 cut(s) 85, 236, 452, 727
HpySE526I ACGT 2 cut(s) 501, 651
Hsp92II CATG 2 cut(s) 35, 479
HspAI GCGC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1068
LguI GCTCTTC 1 cut(s) 604
LmnI GCTCC 2 cut(s) 67, 595
Lsp1109I GCAGC 4 cut(s) 194, 483, 973, 976
LweI GCATC 1 cut(s) 690
MaeI CTAG 5 cut(s) 120, 176, 831, 1017, 1023
MaeII ACGT 2 cut(s) 501, 651
MaeIII GTNAC 2 cut(s) 252, 826
MalI GATC 9 cut(s) 6, 231, 332, 337, 458, 579, 659, 690, 1070
MboI GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1068
MboII GAAGA 6 cut(s) 30, 284, 382, 452, 591, 1044
MfeI CAATTG 3 cut(s) 138, 993, 1047
MflI RGATCY 2 cut(s) 4, 229
MhlI GDGCHC 3 cut(s) 318, 599, 798
MlyI GAGTC 4 cut(s) 91, 634, 908, 1035
MmeI TCCRAC 3 cut(s) 579, 638, 910
MroXI GAANNNNTTC 1 cut(s) 1036
MspI CCGG 4 cut(s) 262, 296, 565, 683
MspR9I CCNGG 4 cut(s) 78, 297, 565, 621
MunI CAATTG 3 cut(s) 138, 993, 1047
MvaI CCWGG 2 cut(s) 78, 621
MvnI CGCG 2 cut(s) 267, 521
MwoI GCNNNNNNNGC 2 cut(s) 692, 709
NciI CCSGG 2 cut(s) 297, 565
NdeII GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1068
NlaIII CATG 2 cut(s) 35, 479
NlaIV GGNNCC 3 cut(s) 221, 569, 591
NmuCI GTSAC 1 cut(s) 252
PasI CCCWGGG 1 cut(s) 620
PciSI GCTCTTC 1 cut(s) 604
PcsI WCGNNNNNNNCGW 3 cut(s) 582, 648, 657
PdmI GAANNNNTTC 1 cut(s) 1036
PfeI GAWTC 2 cut(s) 28, 1043
PflMI CCANNNNNTGG 2 cut(s) 283, 620
PfoI TCCNGGA 1 cut(s) 76
PkrI GCNGC 4 cut(s) 209, 473, 963, 966
PleI GAGTC 4 cut(s) 91, 633, 908, 1034
PpsI GAGTC 4 cut(s) 91, 633, 908, 1034
Ppu21I YACGTR 1 cut(s) 652
Psp124BI GAGCTC 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 76, 619
PspGI CCWGG 2 cut(s) 76, 619
PspN4I GGNNCC 3 cut(s) 221, 569, 591
PspPI GGNCC 1 cut(s) 747
PstI CTGCAG 1 cut(s) 473
PsuI RGATCY 2 cut(s) 4, 229
RsaI GTAC 2 cut(s) 290, 1015
RsaNI GTAC 2 cut(s) 289, 1014
SacI GAGCTC 1 cut(s) 599
SapI GCTCTTC 1 cut(s) 604
SatI GCNGC 4 cut(s) 208, 472, 962, 965
Sau3AI GATC 9 cut(s) 4, 229, 330, 335, 456, 577, 657, 688, 1068
Sau96I GGNCC 1 cut(s) 747
ScaI AGTACT 1 cut(s) 1015
SchI GAGTC 4 cut(s) 91, 634, 908, 1035
ScrFI CCNGG 4 cut(s) 78, 297, 565, 621
SduI GDGCHC 3 cut(s) 318, 599, 798
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 747
SpeI ACTAGT 2 cut(s) 830, 1016
SsiI CCGC 2 cut(s) 530, 686
SspMI CTAG 5 cut(s) 120, 176, 831, 1017, 1023
SstI GAGCTC 1 cut(s) 599
StyD4I CCNGG 4 cut(s) 76, 295, 563, 619
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 2 cut(s) 224, 252
TaiI ACGT 2 cut(s) 504, 654
TaqI TCGA 4 cut(s) 318, 576, 660, 1071
TatI WGTACW 2 cut(s) 288, 1013
TfiI GAWTC 2 cut(s) 28, 1043
TscAI CASTG 3 cut(s) 255, 747, 985
TseFI GTSAC 1 cut(s) 252
TseI GCWGC 4 cut(s) 207, 471, 961, 964
Tsp45I GTSAC 1 cut(s) 252
TspDTI ATGAA 5 cut(s) 20, 30, 95, 336, 540
TspRI CASTG 3 cut(s) 255, 747, 985
Van91I CCANNNNNTGG 2 cut(s) 283, 620
VpaK11BI GGWCC 1 cut(s) 747
XbaI TCTAGA 1 cut(s) 1022
XmnI GAANNNNTTC 1 cut(s) 1036
XspI CTAG 5 cut(s) 120, 176, 831, 1017, 1023
ZrmI AGTACT 1 cut(s) 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.