Rw4G003340

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014022
Physical Location & Seq
Reverse (-)
6684512 .. 6685620
1109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G003340.1

Sequence Viewer

Length: 1074 bp
ATGGATCTTGTAAGAAAAGGCTACGTGATTCATGTCTCTTCATCAGAAGCACAAGCACTTGCTCCAATTACAAGTCCAGGAAGCTCAATCTTTGGGACTCCTATTCACCATTCAGACACAAGCTTCCCCATTATAGCAATTGCCATAATAGGAATTATAGCCACGGCTTTCTTGCTAGTGAGCTACTACATCTTTGTCATCAAGTGCTGCCTCAACTGGCACCGTGTGGATCTCCTCAGGCGATTTTCACTGTCACGAAACCGGCGCGACGAAGACCAGCTAATGGTGTACTCGCCGGGTTTGGAGACCCGAGGGCTCGATGAAGCTGTGATCAGATCCATCCCATTGTTGCAGTTCAAGAAAGAAGGGAAGAACAGAGGTGGGTTTGAACAAGGAAGCTTGTTTTGTGAGTGTGCTGTTTGCTTGAATGAGTTTCAAGAAGATGAGAAGCTGAGGATCATACCCAACTGCAGCCATGTTTTCCACATTGACTGCATTGACGTTTGGCTTCAAAACAACGCGAATTGCCCGCTTTGTAGAACAAGCATTTCATCCAACACAACTCGGTTCCAATTCGATCAAATCGTGGCTCCGAGCTCTTCACCTCAAAATCCAACCCCTGGGAGTCTCGATGGAGGCGACGACGACTTCGTGGTGATCGAATTGAGCAACGAAAACAATCCGGCGGGTCAAGCGTTGATGCTTGGAGTGCAAGAAAAATTGAACTCAGGGGAGTTGTCAGTGAGGTCCATTAGTCCTTCCCCAAGGAAATTGGAACAAATTAGAACATTTGTGCCCAAAAAGGCAAGAAAGTTTCACAACAAGGGGCAAAGATGGCAATTCTCCATCCAACCCATAAGGAGGTCTTTCTCTATGGACTCATCTAATGATAGACAGCTCTATTTGGCAGTTCAAGAAGCTGTGCAGCAGCAAAGCCAAGTCAGTGAGGTCAGTCCAATTGAAGGTTGCAGTAGCAGTACTAGTTCTAGAGTCAGAAGGTCTTTCTTTTCTTTTGGGCATGGTAGGGGATCTAGAAGTAACAATGCAATTCTGCCTGTTTATTTGGAGCCATAA

Protein Analysis

357

Amino Acids

39.9

Weight (kDa)

8.32

Isoelectric Point (pI)

60.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 137 - 180 6.7e-13 Ring finger domain
zf-RING_11 PF17123 137 - 166 9.9e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 137 - 179 3.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 137 - 181 3.2e-06 zinc-RING finger domain
zf-C3HC4 PF00097 138 - 179 4.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 146 - 180 9.8e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43420
fragaria_vesca FvH4_4g03440
malus_domestica MD13G1212600.v1.1 MD16G1216300.v1.1
prunus_persica Prupe.1G037600_v2.0.a1
pyrus_communis pycom13g18440 pycom16g18090
rosa_chinensis RchiOBHm_Chr4g0392431
rosa_laevigata RLG00000009807
rosa_multiflora Rmu_sc0001800.1_g000001
rosa_roxburghii Rroxscaffold_5G00337650
rosa_rugosa Rorug03G0338800
rosa_samantha Rh4AG042800 Rh4BG037800 Rh4CG045800 Rh4DG039900
rosa_wichuraiana Rw4G003340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 219
AccB7I CCANNNNNTGG 2 cut(s) 283, 620
AccII CGCG 2 cut(s) 267, 521
AciI CCGC 2 cut(s) 530, 686
AclWI GGATC 5 cut(s) 12, 237, 330, 464, 1036
AdeI CACNNNGTG 1 cut(s) 226
AfaI GTAC 2 cut(s) 290, 979
AfiI CCNNNNNNNGG 4 cut(s) 283, 620, 861, 962
AgsI TTSAA 8 cut(s) 358, 389, 427, 437, 512, 724, 914, 962
AhlI ACTAGT 1 cut(s) 980
AjnI CCWGG 2 cut(s) 76, 619
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 3 cut(s) 40, 299, 632
AlwI GGATC 5 cut(s) 12, 237, 330, 464, 1036
Ama87I CYCGRG 1 cut(s) 309
ApeKI GCWGC 4 cut(s) 207, 471, 925, 928
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 747
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 3 cut(s) 98, 594, 667
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 1 cut(s) 747
AxyI CCTNAGG 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 798
BanI GGYRCC 1 cut(s) 219
BanII GRGCYC 2 cut(s) 318, 599
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 599
BbvCI CCTCAGC 1 cut(s) 452
BbvI GCAGC 4 cut(s) 194, 483, 937, 940
BccI CCATC 4 cut(s) 347, 626, 828, 854
BceAI ACGGC 1 cut(s) 180
BciT130I CCWGG 2 cut(s) 78, 621
BclI TGATCA 1 cut(s) 330
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 3 cut(s) 40, 299, 632
BcuI ACTAGT 1 cut(s) 980
BfaI CTAG 4 cut(s) 176, 981, 987, 1032
BfmI CTRYAG 1 cut(s) 469
BisI GCNGC 4 cut(s) 208, 472, 926, 929
BlsI GCNGC 4 cut(s) 209, 473, 927, 930
BmcAI AGTACT 1 cut(s) 979
Bme1390I CCNGG 3 cut(s) 78, 297, 621
Bme18I GGWCC 1 cut(s) 747
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 747
BmiI GGNNCC 4 cut(s) 221, 569, 591, 1068
BmrFI CCNGG 3 cut(s) 78, 297, 621
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 279
Bpu10I CCTNAGC 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 25
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 5 cut(s) 162, 310, 619, 620, 764
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 620, 861, 962
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 221
Bse21I CCTNAGG 1 cut(s) 236
BseBI CCWGG 2 cut(s) 78, 621
BseDI CCNNGG 5 cut(s) 162, 310, 619, 620, 764
BseGI GGATG 3 cut(s) 339, 551, 846
BseLI CCNNNNNNNGG 4 cut(s) 283, 620, 861, 962
BseMII CTCAG 3 cut(s) 250, 443, 741
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 1 cut(s) 224
BseSI GKGCMC 1 cut(s) 798
BseXI GCAGC 4 cut(s) 194, 483, 937, 940
BsgI GTGCAG 1 cut(s) 944
Bsh1236I CGCG 2 cut(s) 267, 521
BshNI GGYRCC 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 599
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 3 cut(s) 262, 296, 683
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 4 cut(s) 283, 620, 861, 962
BsmAI GTCTC 3 cut(s) 40, 299, 632
BsmFI GGGAC 1 cut(s) 109
Bso31I GGTCTC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 3 cut(s) 318, 599, 798
Bsp143I GATC 8 cut(s) 4, 229, 330, 335, 456, 577, 657, 1028
BspACI CCGC 2 cut(s) 530, 686
BspCNI CTCAG 3 cut(s) 249, 444, 740
BspFNI CGCG 2 cut(s) 267, 521
BspLI GGNNCC 4 cut(s) 221, 569, 591, 1068
BspMAI CTGCAG 1 cut(s) 473
BspPI GGATC 5 cut(s) 12, 237, 330, 464, 1036
BspQI GCTCTTC 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 219
BspTNI GGTCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 5 cut(s) 162, 310, 619, 620, 764
BssMI GATC 8 cut(s) 4, 229, 330, 335, 456, 577, 657, 1028
BssT1I CCWWGG 1 cut(s) 764
Bst2UI CCWGG 2 cut(s) 78, 621
Bst4CI ACNGT 2 cut(s) 224, 252
Bst6I CTCTTC 2 cut(s) 43, 604
BstBAI YACGTR 1 cut(s) 25
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 3 cut(s) 236, 452, 727
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 3 cut(s) 339, 551, 846
BstFNI CGCG 2 cut(s) 267, 521
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 8 cut(s) 7, 232, 333, 338, 459, 580, 660, 1031
BstMAI GTCTC 3 cut(s) 40, 299, 632
BstMBI GATC 8 cut(s) 4, 229, 330, 335, 456, 577, 657, 1028
BstMWI GCNNNNNNNGC 3 cut(s) 692, 709, 835
BstNI CCWGG 2 cut(s) 78, 621
BstSCI CCNGG 3 cut(s) 76, 295, 619
BstSFI CTRYAG 1 cut(s) 469
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 2 cut(s) 267, 521
BstV1I GCAGC 4 cut(s) 194, 483, 937, 940
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 4 cut(s) 4, 229, 335, 1028
BstYI RGATCY 4 cut(s) 4, 229, 335, 1028
Bsu36I CCTNAGG 1 cut(s) 236
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 3 cut(s) 339, 551, 846
BtsIMutI CAGTG 3 cut(s) 248, 747, 949
Cac8I GCNNGC 1 cut(s) 530
CfoI GCGC 1 cut(s) 267
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 747
Csp6I GTAC 2 cut(s) 289, 978
CviAII CATG 3 cut(s) 32, 476, 1019
CviQI GTAC 2 cut(s) 289, 978
DdeI CTNAG 3 cut(s) 236, 452, 727
DpnI GATC 8 cut(s) 6, 231, 332, 337, 458, 579, 659, 1030
DpnII GATC 8 cut(s) 4, 229, 330, 335, 456, 577, 657, 1028
DraIII CACNNNGTG 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 43, 604
EarI CTCTTC 2 cut(s) 43, 604
Ecl136II GAGCTC 1 cut(s) 597
Eco130I CCWWGG 1 cut(s) 764
Eco24I GRGCYC 2 cut(s) 318, 599
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 747
Eco53kI GAGCTC 1 cut(s) 597
Eco81I CCTNAGG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 597
EcoRII CCWGG 2 cut(s) 76, 619
EcoT14I CCWWGG 1 cut(s) 764
EcoT38I GRGCYC 2 cut(s) 318, 599
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 3 cut(s) 35, 479, 1022
FalI AAGNNNNNCTT 2 cut(s) 850, 882
FaqI GGGAC 1 cut(s) 109
FatI CATG 3 cut(s) 31, 475, 1018
FauI CCCGC 2 cut(s) 537, 679
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 4 cut(s) 208, 472, 926, 929
FokI GGATG 3 cut(s) 326, 538, 833
FriOI GRGCYC 2 cut(s) 318, 599
Fsp4HI GCNGC 4 cut(s) 208, 472, 926, 929
FspBI CTAG 4 cut(s) 176, 981, 987, 1032
GlaI GCGC 1 cut(s) 266
GluI GCNGC 4 cut(s) 208, 472, 926, 929
HapII CCGG 3 cut(s) 262, 296, 683
HhaI GCGC 1 cut(s) 267
Hin1II CATG 3 cut(s) 35, 479, 1022
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HindIII AAGCTT 2 cut(s) 121, 397
HinfI GANTC 5 cut(s) 28, 97, 625, 878, 990
HpaII CCGG 3 cut(s) 262, 296, 683
HphI GGTGA 3 cut(s) 98, 594, 667
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 5 cut(s) 46, 115, 335, 594, 995
Hpy188III TCNNGA 7 cut(s) 255, 358, 437, 629, 914, 987, 1032
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 3 cut(s) 272, 644, 647
HpyAV CCTTC 4 cut(s) 359, 768, 956, 990
HpyCH4III ACNGT 2 cut(s) 224, 252
HpyCH4IV ACGT 2 cut(s) 24, 501
HpyCH4V TGCA 7 cut(s) 352, 471, 495, 712, 925, 969, 1046
HpyF10VI GCNNNNNNNGC 3 cut(s) 692, 709, 835
HpyF3I CTNAG 3 cut(s) 236, 452, 727
HpySE526I ACGT 2 cut(s) 24, 501
Hsp92II CATG 3 cut(s) 35, 479, 1022
HspAI GCGC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 8 cut(s) 4, 229, 330, 335, 456, 577, 657, 1028
LguI GCTCTTC 1 cut(s) 604
LmnI GCTCC 3 cut(s) 67, 595, 1066
Lsp1109I GCAGC 4 cut(s) 194, 483, 937, 940
LweI GCATC 1 cut(s) 690
MaeI CTAG 4 cut(s) 176, 981, 987, 1032
MaeII ACGT 2 cut(s) 24, 501
MaeIII GTNAC 2 cut(s) 252, 1037
MalI GATC 8 cut(s) 6, 231, 332, 337, 458, 579, 659, 1030
MboI GATC 8 cut(s) 4, 229, 330, 335, 456, 577, 657, 1028
MboII GAAGA 5 cut(s) 30, 284, 382, 452, 591
MfeI CAATTG 2 cut(s) 138, 957
MflI RGATCY 4 cut(s) 4, 229, 335, 1028
MhlI GDGCHC 3 cut(s) 318, 599, 798
MlyI GAGTC 4 cut(s) 91, 634, 872, 999
MmeI TCCRAC 3 cut(s) 579, 638, 874
MspI CCGG 3 cut(s) 262, 296, 683
MspR9I CCNGG 3 cut(s) 78, 297, 621
MunI CAATTG 2 cut(s) 138, 957
MvaI CCWGG 2 cut(s) 78, 621
MvnI CGCG 2 cut(s) 267, 521
MwoI GCNNNNNNNGC 3 cut(s) 692, 709, 835
NciI CCSGG 1 cut(s) 297
NdeII GATC 8 cut(s) 4, 229, 330, 335, 456, 577, 657, 1028
NlaIII CATG 3 cut(s) 35, 479, 1022
NlaIV GGNNCC 4 cut(s) 221, 569, 591, 1068
NmuCI GTSAC 1 cut(s) 252
PasI CCCWGGG 1 cut(s) 620
PciSI GCTCTTC 1 cut(s) 604
PcsI WCGNNNNNNNCGW 4 cut(s) 582, 636, 648, 657
PfeI GAWTC 1 cut(s) 28
PflMI CCANNNNNTGG 2 cut(s) 283, 620
PfoI TCCNGGA 1 cut(s) 76
PkrI GCNGC 4 cut(s) 209, 473, 927, 930
PleI GAGTC 4 cut(s) 91, 633, 872, 998
PpsI GAGTC 4 cut(s) 91, 633, 872, 998
Ppu21I YACGTR 1 cut(s) 25
Psp124BI GAGCTC 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 76, 619
PspGI CCWGG 2 cut(s) 76, 619
PspN4I GGNNCC 4 cut(s) 221, 569, 591, 1068
PspPI GGNCC 1 cut(s) 747
PstI CTGCAG 1 cut(s) 473
PsuI RGATCY 4 cut(s) 4, 229, 335, 1028
RsaI GTAC 2 cut(s) 290, 979
RsaNI GTAC 2 cut(s) 289, 978
SacI GAGCTC 1 cut(s) 599
SapI GCTCTTC 1 cut(s) 604
SatI GCNGC 4 cut(s) 208, 472, 926, 929
Sau3AI GATC 8 cut(s) 4, 229, 330, 335, 456, 577, 657, 1028
Sau96I GGNCC 1 cut(s) 747
ScaI AGTACT 1 cut(s) 979
SchI GAGTC 4 cut(s) 91, 634, 872, 999
ScrFI CCNGG 3 cut(s) 78, 297, 621
SduI GDGCHC 3 cut(s) 318, 599, 798
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 747
SpeI ACTAGT 1 cut(s) 980
SsiI CCGC 2 cut(s) 530, 686
SspMI CTAG 4 cut(s) 176, 981, 987, 1032
SstI GAGCTC 1 cut(s) 599
StyD4I CCNGG 3 cut(s) 76, 295, 619
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 2 cut(s) 224, 252
TaiI ACGT 2 cut(s) 27, 504
TaqI TCGA 4 cut(s) 318, 576, 630, 660
TatI WGTACW 2 cut(s) 288, 977
TfiI GAWTC 1 cut(s) 28
TscAI CASTG 3 cut(s) 255, 747, 949
TseFI GTSAC 1 cut(s) 252
TseI GCWGC 4 cut(s) 207, 471, 925, 928
Tsp45I GTSAC 1 cut(s) 252
TspDTI ATGAA 4 cut(s) 20, 30, 336, 540
TspRI CASTG 3 cut(s) 255, 747, 949
Van91I CCANNNNNTGG 2 cut(s) 283, 620
VpaK11BI GGWCC 1 cut(s) 747
XbaI TCTAGA 2 cut(s) 986, 1031
XspI CTAG 4 cut(s) 176, 981, 987, 1032
ZrmI AGTACT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.