FvH4_4g04200

50S ribosomal protein L28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009333
Physical Location & Seq
Forward (+)
3630440 .. 3631719
1280 bp
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UTR
Exon/CDS
Intron
FvH4_4g04200.t1

Sequence Viewer

Length: 453 bp
ATGGCAGCTTCCGCAACCGCGGCTGTGTTCTCCGGTCTATGCTTCCGCCGCGGATCATCAGACTCCTCCAAGAGATTGTCTTCCAAAACCCAAGCAGTTTCCGATGTTGGGTTCGTCACCAGTCAGCTCAGTGGCCTCAAAATCTCCCACGACATGTCGTCTCAGCTCCCCAGTCCAATCTTCACTCCTTCCCTCCCTGGCCTTCAGCCCATTGTTGCGCGTAGAATTTGTCCTTTTACTGGGAAGAAAGCAAACAAGGCAAACAAGATTTCCTTCTCGGCCCACAAGACCAAGAAGTTGCAGTTTGTGAATCTGCAGTACAAGAGGATTTGGTGGGAAGGAGGGAAGCGGTTTTTGAAGCTGAGGTTGTCGACCAAAGCAATCAAGACCATAGAGAAGAATGGACTGGAAGCCGTTGCCAAGAAGGCCGGGATAGATCTTCGCAAGTGTTGA

Protein Analysis

151

Amino Acids

16.4

Weight (kDa)

10.69

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28 PF00830 75 - 134 8.8e-19 Ribosomal L28 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 371
AccII CGCG 3 cut(s) 20, 51, 220
AciI CCGC 7 cut(s) 12, 18, 20, 46, 49, 51, 349
AclWI GGATC 1 cut(s) 61
AcsI RAATTY 1 cut(s) 225
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 2 cut(s) 108, 239
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 1 cut(s) 358
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 4 cut(s) 8, 127, 166, 361
AluI AGCT 4 cut(s) 8, 127, 166, 361
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 1 cut(s) 61
AoxI GGCC 4 cut(s) 133, 199, 279, 426
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 225
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 280
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 1 cut(s) 17
BceAI ACGGC 1 cut(s) 398
BciT130I CCWGG 1 cut(s) 198
BcnI CCSGG 1 cut(s) 430
BcoDI GTCTC 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 314
BglI GCCNNNNNGGC 1 cut(s) 425
BglII AGATCT 1 cut(s) 436
BisI GCNGC 3 cut(s) 6, 21, 49
BlsI GCNGC 3 cut(s) 7, 22, 50
Bme1390I CCNGG 2 cut(s) 198, 430
BmeRI GACNNNNNGTC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 280
BmrFI CCNGG 2 cut(s) 198, 430
BmrI ACTGGG 2 cut(s) 165, 249
BmuI ACTGGG 2 cut(s) 165, 249
BpiI GAAGAC 1 cut(s) 72
Bpu10I CCTNAGC 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 430
BsaJI CCNNGG 3 cut(s) 18, 49, 196
BsaWI WCCGGW 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 239
Bse1I ACTGG 4 cut(s) 120, 171, 244, 411
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 18, 49, 196
BseLI CCNNNNNNNGG 2 cut(s) 108, 239
BseMII CTCAG 3 cut(s) 142, 176, 353
BseNI ACTGG 4 cut(s) 120, 171, 244, 411
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 3 cut(s) 20, 51, 220
BshFI GGCC 4 cut(s) 135, 201, 281, 428
BsiSI CCGG 2 cut(s) 33, 429
BslI CCNNNNNNNGG 2 cut(s) 108, 239
BsmAI GTCTC 1 cut(s) 165
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 4 cut(s) 135, 201, 281, 428
Bsp143I GATC 2 cut(s) 53, 436
BspACI CCGC 7 cut(s) 12, 18, 20, 46, 49, 51, 349
BspANI GGCC 4 cut(s) 135, 201, 281, 428
BspCNI CTCAG 3 cut(s) 141, 175, 354
BspFNI CGCG 3 cut(s) 20, 51, 220
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 1 cut(s) 61
BsrI ACTGG 4 cut(s) 120, 171, 244, 411
BssECI CCNNGG 3 cut(s) 18, 49, 196
BssMI GATC 2 cut(s) 53, 436
Bst2UI CCWGG 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 128, 162, 362
BstDSI CCRYGG 2 cut(s) 18, 49
BstFNI CGCG 3 cut(s) 20, 51, 220
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 2 cut(s) 56, 439
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 2 cut(s) 53, 436
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 48, 257, 425
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 2 cut(s) 196, 428
BstSFI CTRYAG 1 cut(s) 314
BstUI CGCG 3 cut(s) 20, 51, 220
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 4 cut(s) 135, 201, 281, 428
BtgI CCRYGG 2 cut(s) 18, 49
BtsIMutI CAGTG 1 cut(s) 136
CfoI GCGC 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 280
Cfr42I CCGCGG 2 cut(s) 21, 52
Csp6I GTAC 1 cut(s) 319
CviAII CATG 1 cut(s) 154
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 3 cut(s) 128, 162, 362
DpnI GATC 2 cut(s) 55, 438
DpnII GATC 2 cut(s) 53, 436
DriI GACNNNNNGTC 1 cut(s) 157
Eam1105I GACNNNNNGTC 1 cut(s) 157
EciI GGCGGA 1 cut(s) 35
Eco57I CTGAAG 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 1 cut(s) 157
FaiI YATR 3 cut(s) 40, 155, 392
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FatI CATG 1 cut(s) 153
FblI GTMKAC 1 cut(s) 371
Fnu4HI GCNGC 3 cut(s) 6, 21, 49
Fsp4HI GCNGC 3 cut(s) 6, 21, 49
GlaI GCGC 1 cut(s) 219
GluI GCNGC 3 cut(s) 6, 21, 49
HaeIII GGCC 4 cut(s) 135, 201, 281, 428
HapII CCGG 2 cut(s) 33, 429
HhaI GCGC 1 cut(s) 220
Hin1II CATG 1 cut(s) 157
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HinfI GANTC 2 cut(s) 62, 310
HpaII CCGG 2 cut(s) 33, 429
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 372
Hpy188I TCNGA 2 cut(s) 61, 103
Hpy188III TCNNGA 1 cut(s) 385
Hpy8I GTNNAC 1 cut(s) 372
HpyAV CCTTC 5 cut(s) 198, 212, 283, 332, 418
HpyCH4V TGCA 2 cut(s) 301, 316
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 48, 257, 425
HpyF3I CTNAG 3 cut(s) 128, 162, 362
Hsp92II CATG 1 cut(s) 157
HspAI GCGC 1 cut(s) 218
KspI CCGCGG 2 cut(s) 21, 52
Kzo9I GATC 2 cut(s) 53, 436
LmnI GCTCC 1 cut(s) 171
LpnPI CCDG 8 cut(s) 46, 133, 183, 184, 210, 225, 392, 442
Lsp1109I GCAGC 1 cut(s) 17
MaeIII GTNAC 1 cut(s) 115
MalI GATC 2 cut(s) 55, 438
MboI GATC 2 cut(s) 53, 436
MboII GAAGA 5 cut(s) 72, 172, 256, 409, 431
MflI RGATCY 1 cut(s) 436
MluCI AATT 1 cut(s) 225
MlyI GAGTC 1 cut(s) 56
MnlI CCTC 6 cut(s) 76, 146, 203, 318, 335, 357
MspA1I CMGCKG 2 cut(s) 20, 51
MspI CCGG 2 cut(s) 33, 429
MspR9I CCNGG 2 cut(s) 198, 430
MvaI CCWGG 1 cut(s) 198
MvnI CGCG 3 cut(s) 20, 51, 220
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 48, 257, 425
NciI CCSGG 1 cut(s) 430
NdeII GATC 2 cut(s) 53, 436
NlaIII CATG 1 cut(s) 157
NmeAIII GCCGAG 1 cut(s) 257
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 1 cut(s) 157
PciI ACATGT 1 cut(s) 153
PfeI GAWTC 1 cut(s) 310
PkrI GCNGC 3 cut(s) 7, 22, 50
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
PscI ACATGT 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspPI GGNCC 1 cut(s) 280
PstI CTGCAG 1 cut(s) 318
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacII CCGCGG 2 cut(s) 21, 52
SalI GTCGAC 1 cut(s) 370
SatI GCNGC 3 cut(s) 6, 21, 49
Sau3AI GATC 2 cut(s) 53, 436
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 2 cut(s) 198, 430
SetI ASST 5 cut(s) 10, 129, 168, 363, 368
SfcI CTRYAG 1 cut(s) 314
Sfr303I CCGCGG 2 cut(s) 21, 52
SgrBI CCGCGG 2 cut(s) 21, 52
Sse9I AATT 1 cut(s) 225
SsiI CCGC 7 cut(s) 12, 18, 20, 46, 49, 51, 349
StyD4I CCNGG 2 cut(s) 196, 428
TaqI TCGA 1 cut(s) 371
TasI AATT 1 cut(s) 225
TatI WGTACW 1 cut(s) 318
TauI GCSGC 2 cut(s) 23, 51
TfiI GAWTC 1 cut(s) 310
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 115
TspRI CASTG 1 cut(s) 136
XapI RAATTY 1 cut(s) 225
XceI RCATGY 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.