Rroxscaffold_5G00339690

50S ribosomal protein L28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009333
Physical Location & Seq
Forward (+)
8127315 .. 8128803
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00339690.1

Sequence Viewer

Length: 465 bp
ATGGCAGCTTCCGCAACCGCGGCTGTCTTCTTCGGCAGCAGTTCCGGTCTATGCCTCCGCCGCGGAGCAGAGTCCTCCAAGAGAATATTGTCTTCCAAGACCCAAGCAGTTTCCGATATTGGGTTCGTCACCAGTCAGCTCAGCGGCCTCAGAATCTCCTACGACGTGTCGTCTCAGCTCCCCAGTCCCATCTTCACTCCTTCCCTCCCTGGCCTTCAGCCCATTGTTGCTCGTAGAATTTGTCCTTTTACTGGGAAGAAAGCAAACAAGGCAAACAAGATTTCATTCTCAGCACACAAGACCAAGAAGTTGCAGTTTGTGAACCTGCAGTACAAGAGGATTTGGTGGGAAGGCGGGAAGCGCTTTTTGAAGCTGCGCTTGTCAACCAAAGCAATCAAGACCATAGAGAAGAATGGACTGGAAGCCGTTGCCAAGAAGGCCGGGATAGATCTTCGGAAGTGTTGA

Protein Analysis

154

Amino Acids

16.8

Weight (kDa)

10.69

Isoelectric Point (pI)

59.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28 PF00830 79 - 138 9.3e-19 Ribosomal L28 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 333
AccII CGCG 2 cut(s) 20, 63
AciI CCGC 8 cut(s) 12, 18, 20, 58, 61, 63, 144, 354
AcsI RAATTY 1 cut(s) 237
AcuI CTGAAG 1 cut(s) 200
AfaI GTAC 1 cut(s) 332
AfeI AGCGCT 1 cut(s) 362
AfiI CCNNNNNNNGG 2 cut(s) 120, 251
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 1 cut(s) 370
AhdI GACNNNNNGTC 1 cut(s) 169
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 4 cut(s) 8, 139, 178, 373
AluI AGCT 4 cut(s) 8, 139, 178, 373
Alw26I GTCTC 1 cut(s) 177
Aor51HI AGCGCT 1 cut(s) 362
AoxI GGCC 3 cut(s) 145, 211, 438
ApeKI GCWGC 3 cut(s) 5, 36, 373
ApoI RAATTY 1 cut(s) 237
AspLEI GCGC 2 cut(s) 363, 378
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 1 cut(s) 121
BbsI GAAGAC 2 cut(s) 19, 84
BbvI GCAGC 3 cut(s) 17, 48, 360
BccI CCATC 1 cut(s) 197
BceAI ACGGC 1 cut(s) 410
BciT130I CCWGG 1 cut(s) 210
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 1 cut(s) 177
BfmI CTRYAG 1 cut(s) 326
BfoI RGCGCY 1 cut(s) 364
BfuAI ACCTGC 1 cut(s) 333
BglI GCCNNNNNGGC 1 cut(s) 437
BglII AGATCT 1 cut(s) 448
BisI GCNGC 6 cut(s) 6, 21, 37, 61, 145, 374
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 6 cut(s) 7, 22, 38, 62, 146, 375
Bme1390I CCNGG 2 cut(s) 210, 442
BmeRI GACNNNNNGTC 1 cut(s) 169
BmgBI CACGTC 1 cut(s) 166
BmrFI CCNGG 2 cut(s) 210, 442
BmrI ACTGGG 2 cut(s) 177, 261
BmuI ACTGGG 2 cut(s) 177, 261
BpiI GAAGAC 2 cut(s) 19, 84
Bpu1102I GCTNAGC 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 442
BsaJI CCNNGG 3 cut(s) 18, 61, 208
BsaWI WCCGGW 1 cut(s) 44
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 251
Bse1I ACTGG 4 cut(s) 132, 183, 256, 423
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 3 cut(s) 18, 61, 208
BseLI CCNNNNNNNGG 2 cut(s) 120, 251
BseMII CTCAG 4 cut(s) 154, 163, 188, 303
BseNI ACTGG 4 cut(s) 132, 183, 256, 423
BseXI GCAGC 3 cut(s) 17, 48, 360
Bsh1236I CGCG 2 cut(s) 20, 63
BshFI GGCC 3 cut(s) 147, 213, 440
BsiSI CCGG 2 cut(s) 45, 441
BslFI GGGAC 1 cut(s) 171
BslI CCNNNNNNNGG 2 cut(s) 120, 251
BsmAI GTCTC 1 cut(s) 177
BsmBI CGTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 3 cut(s) 147, 213, 440
Bsp143I GATC 1 cut(s) 448
Bsp1720I GCTNAGC 1 cut(s) 140
BspACI CCGC 8 cut(s) 12, 18, 20, 58, 61, 63, 144, 354
BspANI GGCC 3 cut(s) 147, 213, 440
BspCNI CTCAG 4 cut(s) 153, 162, 187, 302
BspFNI CGCG 2 cut(s) 20, 63
BspMAI CTGCAG 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 333
BsrI ACTGG 4 cut(s) 132, 183, 256, 423
BssECI CCNNGG 3 cut(s) 18, 61, 208
BssMI GATC 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 210
BstDEI CTNAG 4 cut(s) 140, 149, 174, 289
BstDSI CCRYGG 2 cut(s) 18, 61
BstFNI CGCG 2 cut(s) 20, 63
BstH2I RGCGCY 1 cut(s) 364
BstHHI GCGC 2 cut(s) 363, 378
BstKTI GATC 1 cut(s) 451
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 1 cut(s) 448
BstMWI GCNNNNNNNGC 6 cut(s) 11, 20, 60, 269, 360, 437
BstNI CCWGG 1 cut(s) 210
BstSCI CCNGG 2 cut(s) 208, 440
BstSFI CTRYAG 1 cut(s) 326
BstUI CGCG 2 cut(s) 20, 63
BstV1I GCAGC 3 cut(s) 17, 48, 360
BstV2I GAAGAC 2 cut(s) 19, 84
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 3 cut(s) 147, 213, 440
BtgI CCRYGG 2 cut(s) 18, 61
BtrI CACGTC 1 cut(s) 166
BveI ACCTGC 1 cut(s) 333
CfoI GCGC 2 cut(s) 363, 378
Cfr42I CCGCGG 2 cut(s) 21, 64
Csp6I GTAC 1 cut(s) 331
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 4 cut(s) 140, 149, 174, 289
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
DriI GACNNNNNGTC 1 cut(s) 169
Eam1105I GACNNNNNGTC 1 cut(s) 169
EciI GGCGGA 1 cut(s) 47
Eco47III AGCGCT 1 cut(s) 362
Eco57I CTGAAG 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 208
Esp3I CGTCTC 1 cut(s) 177
FaiI YATR 2 cut(s) 52, 404
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FaqI GGGAC 1 cut(s) 171
FauI CCCGC 1 cut(s) 347
Fnu4HI GCNGC 6 cut(s) 6, 21, 37, 61, 145, 374
Fsp4HI GCNGC 6 cut(s) 6, 21, 37, 61, 145, 374
GlaI GCGC 2 cut(s) 362, 377
GluI GCNGC 6 cut(s) 6, 21, 37, 61, 145, 374
HaeII RGCGCY 1 cut(s) 364
HaeIII GGCC 3 cut(s) 147, 213, 440
HapII CCGG 2 cut(s) 45, 441
HhaI GCGC 2 cut(s) 363, 378
Hin6I GCGC 2 cut(s) 361, 376
HinP1I GCGC 2 cut(s) 361, 376
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 2 cut(s) 71, 153
HpaII CCGG 2 cut(s) 45, 441
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 322, 384
Hpy188I TCNGA 3 cut(s) 115, 152, 456
Hpy188III TCNNGA 1 cut(s) 397
Hpy8I GTNNAC 2 cut(s) 322, 384
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 4 cut(s) 210, 224, 344, 430
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 2 cut(s) 313, 328
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 20, 60, 269, 360, 437
HpyF3I CTNAG 4 cut(s) 140, 149, 174, 289
HpySE526I ACGT 1 cut(s) 165
HspAI GCGC 2 cut(s) 361, 376
KspI CCGCGG 2 cut(s) 21, 64
Kzo9I GATC 1 cut(s) 448
LmnI GCTCC 2 cut(s) 65, 183
LpnPI CCDG 9 cut(s) 58, 145, 195, 196, 222, 237, 338, 404, 454
Lsp1109I GCAGC 3 cut(s) 17, 48, 360
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 127
MalI GATC 1 cut(s) 450
MboI GATC 1 cut(s) 448
MboII GAAGA 7 cut(s) 19, 22, 84, 184, 268, 421, 443
MflI RGATCY 1 cut(s) 448
MluCI AATT 1 cut(s) 237
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 5 cut(s) 65, 85, 158, 215, 330
MspA1I CMGCKG 3 cut(s) 20, 63, 144
MspI CCGG 2 cut(s) 45, 441
MspR9I CCNGG 2 cut(s) 210, 442
MvaI CCWGG 1 cut(s) 210
MvnI CGCG 2 cut(s) 20, 63
MwoI GCNNNNNNNGC 6 cut(s) 11, 20, 60, 269, 360, 437
NciI CCSGG 1 cut(s) 442
NdeII GATC 1 cut(s) 448
NmuCI GTSAC 1 cut(s) 127
PfeI GAWTC 1 cut(s) 153
PkrI GCNGC 6 cut(s) 7, 22, 38, 62, 146, 375
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 208
PspGI CCWGG 1 cut(s) 208
PsrI GAACNNNNNNTAC 2 cut(s) 314, 346
PstI CTGCAG 1 cut(s) 330
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SacII CCGCGG 2 cut(s) 21, 64
SatI GCNGC 6 cut(s) 6, 21, 37, 61, 145, 374
Sau3AI GATC 1 cut(s) 448
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 2 cut(s) 210, 442
SetI ASST 6 cut(s) 10, 141, 168, 180, 327, 375
SfcI CTRYAG 1 cut(s) 326
Sfr303I CCGCGG 2 cut(s) 21, 64
SgrBI CCGCGG 2 cut(s) 21, 64
Sse9I AATT 1 cut(s) 237
SsiI CCGC 8 cut(s) 12, 18, 20, 58, 61, 63, 144, 354
SspI AATATT 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 208, 440
TaiI ACGT 1 cut(s) 168
TasI AATT 1 cut(s) 237
TatI WGTACW 1 cut(s) 330
TauI GCSGC 3 cut(s) 23, 63, 147
TfiI GAWTC 1 cut(s) 153
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 3 cut(s) 5, 36, 373
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 273
XapI RAATTY 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.