RchiOBHm_Chr4g0394691

50S ribosomal protein L28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009333
Physical Location & Seq
Forward (+)
10378346 .. 10379738
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36716

Sequence Viewer

Length: 453 bp
ATGGCAGCTTCCGCAACCGCGGCTGTGTTCTCCGGTCTATGCTTCCGCCGAGGAGCAGAGTCCTCCAAGAGAATATTGTCTTCCAAGACCCAAGCAGTTTCCGATATTGGGTTCGTCACCAGTCAGCTCAGTGGCCTCAAAATCTCCTACGACGTGTCGTCTCACCTCCCAACTCCCATCTTCACCCCTTCCCTCCCTGGCCTTCAGCCCATTGTTGCTCGTAGAATTTGTCCGTTTACTGGGAAGAAAGCAAACAAGGCAAACAAGATTTCTTTCTCGGCCCACAAGACCAAGAAGTTGCAGTTTGTGAACCTGCAGTACAAGAGGATTTGGTGGGAAGGTGGGAAGCGCTTTTTGAAGCTGCGCTTGTCAACCAAAGCAATCAAGACCATAGAGAAGAATGGACTGGAAGCCGTTGCCAAGAAGGCTGGGATAGATCTTCGTAAGTGTTGA

Protein Analysis

150

Amino Acids

16.45

Weight (kDa)

10.59

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28 PF00830 75 - 134 8.8e-19 Ribosomal L28 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 321
AccII CGCG 1 cut(s) 20
AciI CCGC 4 cut(s) 12, 18, 20, 46
AcsI RAATTY 1 cut(s) 225
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 1 cut(s) 320
AfeI AGCGCT 1 cut(s) 350
AfiI CCNNNNNNNGG 2 cut(s) 108, 239
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 1 cut(s) 358
AhdI GACNNNNNGTC 1 cut(s) 157
AjiI CACGTC 1 cut(s) 154
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 3 cut(s) 8, 127, 361
AluI AGCT 3 cut(s) 8, 127, 361
Alw26I GTCTC 1 cut(s) 165
Aor51HI AGCGCT 1 cut(s) 350
AoxI GGCC 3 cut(s) 133, 199, 279
ApeKI GCWGC 2 cut(s) 5, 361
ApoI RAATTY 1 cut(s) 225
AspLEI GCGC 2 cut(s) 351, 366
AspS9I GGNCC 1 cut(s) 280
AsuHPI GGTGA 3 cut(s) 109, 155, 175
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 2 cut(s) 17, 348
BccI CCATC 1 cut(s) 185
BceAI ACGGC 1 cut(s) 398
BciT130I CCWGG 1 cut(s) 198
BcoDI GTCTC 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 314
BfoI RGCGCY 1 cut(s) 352
BfuAI ACCTGC 1 cut(s) 321
BglI GCCNNNNNGGC 1 cut(s) 425
BglII AGATCT 1 cut(s) 436
BisI GCNGC 3 cut(s) 6, 21, 362
BlsI GCNGC 3 cut(s) 7, 22, 363
Bme1390I CCNGG 1 cut(s) 198
BmeRI GACNNNNNGTC 1 cut(s) 157
BmgBI CACGTC 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 198
BmrI ACTGGG 1 cut(s) 249
BmuI ACTGGG 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 72
BsaJI CCNNGG 3 cut(s) 18, 49, 196
BsaWI WCCGGW 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 239
Bse1I ACTGG 3 cut(s) 120, 244, 411
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 18, 49, 196
BseLI CCNNNNNNNGG 2 cut(s) 108, 239
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 3 cut(s) 120, 244, 411
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 17, 348
BseYI CCCAGC 1 cut(s) 428
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 3 cut(s) 135, 201, 281
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 2 cut(s) 108, 239
BsmAI GTCTC 1 cut(s) 165
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 3 cut(s) 135, 201, 281
Bsp143I GATC 1 cut(s) 436
BspACI CCGC 4 cut(s) 12, 18, 20, 46
BspANI GGCC 3 cut(s) 135, 201, 281
BspCNI CTCAG 1 cut(s) 141
BspFNI CGCG 1 cut(s) 20
BspMAI CTGCAG 1 cut(s) 318
BspMI ACCTGC 1 cut(s) 321
BsrI ACTGG 3 cut(s) 120, 244, 411
BssECI CCNNGG 3 cut(s) 18, 49, 196
BssMI GATC 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 198
BstDEI CTNAG 1 cut(s) 128
BstDSI CCRYGG 1 cut(s) 18
BstFNI CGCG 1 cut(s) 20
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 2 cut(s) 351, 366
BstKTI GATC 1 cut(s) 439
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 1 cut(s) 436
BstMWI GCNNNNNNNGC 4 cut(s) 11, 20, 257, 425
BstNI CCWGG 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 196
BstSFI CTRYAG 1 cut(s) 314
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 17, 348
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 3 cut(s) 135, 201, 281
BtgI CCRYGG 1 cut(s) 18
BtrI CACGTC 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 136
BveI ACCTGC 1 cut(s) 321
CfoI GCGC 2 cut(s) 351, 366
Cfr13I GGNCC 1 cut(s) 280
Cfr42I CCGCGG 1 cut(s) 21
Csp6I GTAC 1 cut(s) 319
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 1 cut(s) 128
DpnI GATC 1 cut(s) 438
DpnII GATC 1 cut(s) 436
DriI GACNNNNNGTC 1 cut(s) 157
Eam1105I GACNNNNNGTC 1 cut(s) 157
EciI GGCGGA 1 cut(s) 35
Eco47III AGCGCT 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 165
FaiI YATR 2 cut(s) 40, 392
FalI AAGNNNNNCTT 2 cut(s) 350, 382
Fnu4HI GCNGC 3 cut(s) 6, 21, 362
Fsp4HI GCNGC 3 cut(s) 6, 21, 362
GlaI GCGC 2 cut(s) 350, 365
GluI GCNGC 3 cut(s) 6, 21, 362
GsaI CCCAGC 1 cut(s) 432
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 3 cut(s) 135, 201, 281
HapII CCGG 1 cut(s) 33
HhaI GCGC 2 cut(s) 351, 366
Hin6I GCGC 2 cut(s) 349, 364
HinP1I GCGC 2 cut(s) 349, 364
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HinfI GANTC 1 cut(s) 59
HpaII CCGG 1 cut(s) 33
HphI GGTGA 3 cut(s) 109, 155, 175
Hpy166II GTNNAC 3 cut(s) 237, 310, 372
Hpy188I TCNGA 1 cut(s) 103
Hpy188III TCNNGA 1 cut(s) 385
Hpy8I GTNNAC 3 cut(s) 237, 310, 372
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 4 cut(s) 198, 212, 332, 418
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 2 cut(s) 301, 316
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 20, 257, 425
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 1 cut(s) 153
HspAI GCGC 2 cut(s) 349, 364
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 1 cut(s) 436
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 8 cut(s) 46, 133, 183, 210, 225, 326, 392, 414
Lsp1109I GCAGC 2 cut(s) 17, 348
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 115
MalI GATC 1 cut(s) 438
MboI GATC 1 cut(s) 436
MboII GAAGA 5 cut(s) 72, 172, 256, 409, 431
MflI RGATCY 1 cut(s) 436
MluCI AATT 1 cut(s) 225
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 6 cut(s) 44, 73, 146, 176, 203, 318
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 198
MvaI CCWGG 1 cut(s) 198
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 4 cut(s) 11, 20, 257, 425
NdeII GATC 1 cut(s) 436
NmeAIII GCCGAG 2 cut(s) 74, 257
NmuCI GTSAC 1 cut(s) 115
PkrI GCNGC 3 cut(s) 7, 22, 363
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 196
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 1 cut(s) 196
PspPI GGNCC 1 cut(s) 280
PsrI GAACNNNNNNTAC 2 cut(s) 302, 334
PstI CTGCAG 1 cut(s) 318
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacII CCGCGG 1 cut(s) 21
SatI GCNGC 3 cut(s) 6, 21, 362
Sau3AI GATC 1 cut(s) 436
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 198
SetI ASST 7 cut(s) 10, 129, 156, 168, 315, 343, 363
SfcI CTRYAG 1 cut(s) 314
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
Sse9I AATT 1 cut(s) 225
SsiI CCGC 4 cut(s) 12, 18, 20, 46
SspI AATATT 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 196
TaiI ACGT 1 cut(s) 156
TasI AATT 1 cut(s) 225
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 23
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 2 cut(s) 5, 361
Tsp45I GTSAC 1 cut(s) 115
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 1 cut(s) 136
XapI RAATTY 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.