MD13G1218400.v1.1

50S ribosomal protein L28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009333
Physical Location & Seq
Reverse (-)
21065995 .. 21067496
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1218400.v1.1.491

Sequence Viewer

Length: 411 bp
ATGCTTCCACAGGGCCGACGCTTCCAAGCGGTTCCTGCAGCCCTTCAAGGCGGAGGCAGTTTCCGACGTCGGTTCGTTACCAGTCAGCTGAGCGGCGTCAGAATCTCCTACGACATGTCGTCTCAGCTGCCAAGTATCTCCACTCCTTCCCTTCCCGGCCTTCAGCCCATCGTTGCCCGTAGAATTTGTCATTTCACTGGGAAGAAAGCAAACAGGGCAAACAAGATCTCCTTCTCAGCCCACAAGACCAAGAAGTTGCAGTTTGTGAACCTGCAGTACAAGAGGGTTTGGTGGGAAGCTGGGAAGCGCTTTCTCAAACTCCGCTTGTCAACCAAAGCATTGAAGACCATAGAGAAGAATGGGATTGATGTTGTTGCCAAGAAGGCCGGGATTGATCTTCGCAAGTTATGA

Protein Analysis

137

Amino Acids

15.29

Weight (kDa)

11.7

Isoelectric Point (pI)

65.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28 PF00830 61 - 120 1.4e-19 Ribosomal L28 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 70
Acc36I ACCTGC 1 cut(s) 279
AccBSI CCGCTC 1 cut(s) 93
AciI CCGC 4 cut(s) 29, 51, 93, 322
AcsI RAATTY 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 2 cut(s) 67, 96
AfaI GTAC 1 cut(s) 278
AfeI AGCGCT 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 2 cut(s) 47, 343
AhdI GACNNNNNGTC 1 cut(s) 118
AluBI AGCT 3 cut(s) 88, 127, 299
AluI AGCT 3 cut(s) 88, 127, 299
Alw26I GTCTC 1 cut(s) 126
Aor51HI AGCGCT 1 cut(s) 308
AoxI GGCC 3 cut(s) 13, 157, 384
ApeKI GCWGC 2 cut(s) 38, 127
ApoI RAATTY 1 cut(s) 183
AspLEI GCGC 1 cut(s) 309
AspS9I GGNCC 1 cut(s) 13
AsuC2I CCSGG 2 cut(s) 156, 388
BbsI GAAGAC 1 cut(s) 350
BbvI GCAGC 2 cut(s) 50, 114
BccI CCATC 1 cut(s) 176
BcnI CCSGG 2 cut(s) 156, 388
BcoDI GTCTC 1 cut(s) 126
BfmI CTRYAG 2 cut(s) 36, 272
BfoI RGCGCY 1 cut(s) 310
BfuAI ACCTGC 1 cut(s) 279
BglI GCCNNNNNGGC 1 cut(s) 383
BglII AGATCT 1 cut(s) 225
BisI GCNGC 3 cut(s) 39, 94, 128
BlpI GCTNAGC 1 cut(s) 89
BlsI GCNGC 3 cut(s) 40, 95, 129
Bme1390I CCNGG 2 cut(s) 156, 388
BmeRI GACNNNNNGTC 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 2 cut(s) 156, 388
BmrI ACTGGG 1 cut(s) 207
BmuI ACTGGG 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 350
Bpu1102I GCTNAGC 1 cut(s) 89
BpuMI CCSGG 2 cut(s) 156, 388
BsaHI GRCGYC 2 cut(s) 67, 96
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bse1I ACTGG 2 cut(s) 81, 202
BseMII CTCAG 3 cut(s) 80, 137, 249
BseNI ACTGG 2 cut(s) 81, 202
BseXI GCAGC 2 cut(s) 50, 114
BseYI CCCAGC 1 cut(s) 299
BshFI GGCC 3 cut(s) 15, 159, 386
BsiSI CCGG 2 cut(s) 156, 387
BsmAI GTCTC 1 cut(s) 126
BsmBI CGTCTC 1 cut(s) 126
BsnI GGCC 3 cut(s) 15, 159, 386
Bsp143I GATC 2 cut(s) 225, 394
Bsp1720I GCTNAGC 1 cut(s) 89
BspACI CCGC 4 cut(s) 29, 51, 93, 322
BspANI GGCC 3 cut(s) 15, 159, 386
BspCNI CTCAG 3 cut(s) 81, 136, 248
BspLI GGNNCC 1 cut(s) 33
BspMAI CTGCAG 2 cut(s) 40, 276
BspMI ACCTGC 1 cut(s) 279
BsrBI CCGCTC 1 cut(s) 93
BsrI ACTGG 2 cut(s) 81, 202
BssMI GATC 2 cut(s) 225, 394
BssNI GRCGYC 2 cut(s) 67, 96
BstACI GRCGYC 2 cut(s) 67, 96
BstDEI CTNAG 3 cut(s) 89, 123, 235
BstH2I RGCGCY 1 cut(s) 310
BstHHI GCGC 1 cut(s) 309
BstKTI GATC 2 cut(s) 228, 397
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 2 cut(s) 225, 394
BstMWI GCNNNNNNNGC 3 cut(s) 35, 215, 383
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 2 cut(s) 154, 386
BstSFI CTRYAG 2 cut(s) 36, 272
BstV1I GCAGC 2 cut(s) 50, 114
BstV2I GAAGAC 1 cut(s) 350
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BsuRI GGCC 3 cut(s) 15, 159, 386
BtsIMutI CAGTG 1 cut(s) 195
BveI ACCTGC 1 cut(s) 279
CfoI GCGC 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 13
CseI GACGC 2 cut(s) 27, 85
Csp6I GTAC 1 cut(s) 277
CviAII CATG 1 cut(s) 115
CviJI RGCY 9 cut(s) 15, 41, 88, 127, 159, 166, 239, 299, 386
CviKI_1 RGCY 9 cut(s) 15, 41, 88, 127, 159, 166, 239, 299, 386
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 3 cut(s) 89, 123, 235
DpnI GATC 2 cut(s) 227, 396
DpnII GATC 2 cut(s) 225, 394
DriI GACNNNNNGTC 1 cut(s) 118
Eam1105I GACNNNNNGTC 1 cut(s) 118
EciI GGCGGA 1 cut(s) 66
Eco47III AGCGCT 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 146
Esp3I CGTCTC 1 cut(s) 126
FaeI CATG 1 cut(s) 118
FaiI YATR 3 cut(s) 116, 350, 409
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FatI CATG 1 cut(s) 114
Fnu4HI GCNGC 3 cut(s) 39, 94, 128
Fsp4HI GCNGC 3 cut(s) 39, 94, 128
GlaI GCGC 1 cut(s) 308
GluI GCNGC 3 cut(s) 39, 94, 128
GsaI CCCAGC 1 cut(s) 303
HaeII RGCGCY 1 cut(s) 310
HaeIII GGCC 3 cut(s) 15, 159, 386
HapII CCGG 2 cut(s) 156, 387
HgaI GACGC 2 cut(s) 27, 85
HhaI GCGC 1 cut(s) 309
Hin1I GRCGYC 2 cut(s) 67, 96
Hin1II CATG 1 cut(s) 118
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 1 cut(s) 102
HpaII CCGG 2 cut(s) 156, 387
Hpy166II GTNNAC 2 cut(s) 268, 330
Hpy188I TCNGA 2 cut(s) 65, 101
Hpy8I GTNNAC 2 cut(s) 268, 330
Hpy99I CGWCG 3 cut(s) 21, 69, 72
HpyAV CCTTC 6 cut(s) 53, 156, 161, 170, 241, 376
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 3 cut(s) 38, 259, 274
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 215, 383
HpyF3I CTNAG 3 cut(s) 89, 123, 235
HpySE526I ACGT 1 cut(s) 67
Hsp92I GRCGYC 2 cut(s) 67, 96
Hsp92II CATG 1 cut(s) 118
HspAI GCGC 1 cut(s) 307
Kzo9I GATC 2 cut(s) 225, 394
LpnPI CCDG 8 cut(s) 48, 94, 169, 183, 199, 284, 285, 400
Lsp1109I GCAGC 2 cut(s) 50, 114
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 1 cut(s) 76
MalI GATC 2 cut(s) 227, 396
MbiI CCGCTC 1 cut(s) 93
MboI GATC 2 cut(s) 225, 394
MboII GAAGA 4 cut(s) 214, 355, 367, 389
MflI RGATCY 1 cut(s) 225
MluCI AATT 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 2 cut(s) 47, 276
MspA1I CMGCKG 2 cut(s) 88, 127
MspI CCGG 2 cut(s) 156, 387
MspR9I CCNGG 2 cut(s) 156, 388
MwoI GCNNNNNNNGC 3 cut(s) 35, 215, 383
NciI CCSGG 2 cut(s) 156, 388
NdeII GATC 2 cut(s) 225, 394
NlaIII CATG 1 cut(s) 118
NlaIV GGNNCC 1 cut(s) 33
NspI RCATGY 1 cut(s) 118
PciI ACATGT 1 cut(s) 114
PfeI GAWTC 1 cut(s) 102
PkrI GCNGC 3 cut(s) 40, 95, 129
PscI ACATGT 1 cut(s) 114
PspFI CCCAGC 1 cut(s) 299
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 13
PsrI GAACNNNNNNTAC 2 cut(s) 260, 292
PstI CTGCAG 2 cut(s) 40, 276
PsuI RGATCY 1 cut(s) 225
PvuII CAGCTG 2 cut(s) 88, 127
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SatI GCNGC 3 cut(s) 39, 94, 128
Sau3AI GATC 2 cut(s) 225, 394
Sau96I GGNCC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 156, 388
SetI ASST 5 cut(s) 70, 90, 129, 273, 301
SfcI CTRYAG 2 cut(s) 36, 272
Sse9I AATT 1 cut(s) 183
SsiI CCGC 4 cut(s) 29, 51, 93, 322
StyD4I CCNGG 2 cut(s) 154, 386
TaiI ACGT 1 cut(s) 70
TasI AATT 1 cut(s) 183
TatI WGTACW 1 cut(s) 276
TauI GCSGC 1 cut(s) 96
TfiI GAWTC 1 cut(s) 102
TscAI CASTG 1 cut(s) 202
TseI GCWGC 2 cut(s) 38, 127
TspRI CASTG 1 cut(s) 202
XapI RAATTY 1 cut(s) 183
XceI RCATGY 1 cut(s) 118
ZraI GACGTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.