pycom13g19330

50S ribosomal protein L28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009333
Physical Location & Seq
Reverse (-)
15317131 .. 15318669
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g19330.1

Sequence Viewer

Length: 465 bp
ATGGCAGCAGCATCGGCAACAGCCGCCGTGTTCTTCGGCACCACCCGAACTCCATGCTTCCATAGGGCCGACGCTTACAAGCGGTTCCTGCAGCCTTTCAAGGCGGAGGCAGTTTCCGACGTCGGGTTCGTTACCAGTCAGCTGAGCGGCGTCAGAATCTCCTACGACATGTCATCTCAGCTGCCAAGTATCTCCACTCCTTCCCTCCCCGCGCTTCAGCCCATCGTTGCCCGTAGAATTTGTCCTTTCACTGGGAAGAAAGCAAACAGGGCAAACAAGATTTCCTTCTCAGCCCACAAGACCAAGAAGTTGCAGTTTGTGAACCTGCAGTACAAGAGGGTTTGGTGGGAAGCTGGGAAGCGCTTTCTCAAACTCCGCTTGTCAACCAAAGCATTGAAGACCATAGAGAAGAATGGGATTGATGTTGTTGCCAAGAAGGCCGGGATTGATCTTCGCAAGTTATGA

Protein Analysis

155

Amino Acids

17.12

Weight (kDa)

10.68

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28 PF00830 79 - 138 1.7e-19 Ribosomal L28 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
Acc36I ACCTGC 1 cut(s) 333
AccB1I GGYRCC 1 cut(s) 38
AccBSI CCGCTC 1 cut(s) 147
AccII CGCG 1 cut(s) 212
AciI CCGC 6 cut(s) 24, 82, 104, 147, 210, 376
AcsI RAATTY 1 cut(s) 237
AcuI CTGAAG 1 cut(s) 200
AcyI GRCGYC 2 cut(s) 120, 150
AfaI GTAC 1 cut(s) 332
AfeI AGCGCT 1 cut(s) 362
AfiI CCNNNNNNNGG 2 cut(s) 123, 251
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 2 cut(s) 100, 397
AluBI AGCT 3 cut(s) 142, 181, 353
AluI AGCT 3 cut(s) 142, 181, 353
Aor51HI AGCGCT 1 cut(s) 362
AoxI GGCC 2 cut(s) 66, 438
ApeKI GCWGC 4 cut(s) 5, 8, 91, 181
ApoI RAATTY 1 cut(s) 237
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AspLEI GCGC 2 cut(s) 214, 363
AspS9I GGNCC 1 cut(s) 66
AsuC2I CCSGG 1 cut(s) 442
BanI GGYRCC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 404
BbvI GCAGC 4 cut(s) 17, 20, 103, 168
BccI CCATC 1 cut(s) 230
BceAI ACGGC 1 cut(s) 11
BcgI CGANNNNNNTGC 3 cut(s) 28, 36, 70
BcnI CCSGG 1 cut(s) 442
BfmI CTRYAG 2 cut(s) 89, 326
BfoI RGCGCY 1 cut(s) 364
BfuAI ACCTGC 1 cut(s) 333
BglI GCCNNNNNGGC 1 cut(s) 437
BisI GCNGC 6 cut(s) 6, 9, 24, 92, 148, 182
BlpI GCTNAGC 1 cut(s) 143
BlsI GCNGC 6 cut(s) 7, 10, 25, 93, 149, 183
Bme1390I CCNGG 1 cut(s) 442
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 2 cut(s) 40, 86
BmrFI CCNGG 1 cut(s) 442
BmrI ACTGGG 1 cut(s) 261
BmsI GCATC 1 cut(s) 20
BmuI ACTGGG 1 cut(s) 261
BpiI GAAGAC 1 cut(s) 404
Bpu1102I GCTNAGC 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 442
BsaHI GRCGYC 2 cut(s) 120, 150
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 251
Bse1I ACTGG 2 cut(s) 135, 256
BseLI CCNNNNNNNGG 2 cut(s) 123, 251
BseMII CTCAG 3 cut(s) 134, 191, 303
BseNI ACTGG 2 cut(s) 135, 256
BseXI GCAGC 4 cut(s) 17, 20, 103, 168
BseYI CCCAGC 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 2 cut(s) 68, 440
BshNI GGYRCC 1 cut(s) 38
BsiSI CCGG 1 cut(s) 441
BslI CCNNNNNNNGG 2 cut(s) 123, 251
BsnI GGCC 2 cut(s) 68, 440
Bsp143I GATC 1 cut(s) 448
Bsp1720I GCTNAGC 1 cut(s) 143
BspACI CCGC 6 cut(s) 24, 82, 104, 147, 210, 376
BspANI GGCC 2 cut(s) 68, 440
BspCNI CTCAG 3 cut(s) 135, 190, 302
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 2 cut(s) 40, 86
BspMAI CTGCAG 2 cut(s) 93, 330
BspMI ACCTGC 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 38
BsrBI CCGCTC 1 cut(s) 147
BsrI ACTGG 2 cut(s) 135, 256
BssMI GATC 1 cut(s) 448
BssNI GRCGYC 2 cut(s) 120, 150
BstACI GRCGYC 2 cut(s) 120, 150
BstDEI CTNAG 3 cut(s) 143, 177, 289
BstFNI CGCG 1 cut(s) 212
BstH2I RGCGCY 1 cut(s) 364
BstHHI GCGC 2 cut(s) 214, 363
BstKTI GATC 1 cut(s) 451
BstMBI GATC 1 cut(s) 448
BstMWI GCNNNNNNNGC 5 cut(s) 14, 23, 88, 269, 437
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 440
BstSFI CTRYAG 2 cut(s) 89, 326
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 4 cut(s) 17, 20, 103, 168
BstV2I GAAGAC 1 cut(s) 404
BsuRI GGCC 2 cut(s) 68, 440
BtsIMutI CAGTG 1 cut(s) 249
BveI ACCTGC 1 cut(s) 333
CfoI GCGC 2 cut(s) 214, 363
Cfr13I GGNCC 1 cut(s) 66
CseI GACGC 2 cut(s) 80, 139
Csp6I GTAC 1 cut(s) 331
CviAII CATG 2 cut(s) 54, 169
CviJI RGCY 9 cut(s) 23, 68, 94, 142, 181, 220, 293, 353, 440
CviKI_1 RGCY 9 cut(s) 23, 68, 94, 142, 181, 220, 293, 353, 440
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 3 cut(s) 143, 177, 289
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
EciI GGCGGA 1 cut(s) 119
Eco47III AGCGCT 1 cut(s) 362
Eco57I CTGAAG 1 cut(s) 200
FaeI CATG 2 cut(s) 57, 172
FaiI YATR 5 cut(s) 55, 63, 170, 404, 463
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FatI CATG 2 cut(s) 53, 168
FauI CCCGC 1 cut(s) 217
Fnu4HI GCNGC 6 cut(s) 6, 9, 24, 92, 148, 182
Fsp4HI GCNGC 6 cut(s) 6, 9, 24, 92, 148, 182
GlaI GCGC 2 cut(s) 213, 362
GluI GCNGC 6 cut(s) 6, 9, 24, 92, 148, 182
GsaI CCCAGC 1 cut(s) 357
HaeII RGCGCY 1 cut(s) 364
HaeIII GGCC 2 cut(s) 68, 440
HapII CCGG 1 cut(s) 441
HgaI GACGC 2 cut(s) 80, 139
HhaI GCGC 2 cut(s) 214, 363
Hin1I GRCGYC 2 cut(s) 120, 150
Hin1II CATG 2 cut(s) 57, 172
Hin6I GCGC 2 cut(s) 212, 361
HinP1I GCGC 2 cut(s) 212, 361
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 1 cut(s) 156
HpaII CCGG 1 cut(s) 441
Hpy166II GTNNAC 2 cut(s) 322, 384
Hpy188I TCNGA 2 cut(s) 118, 155
Hpy8I GTNNAC 2 cut(s) 322, 384
Hpy99I CGWCG 3 cut(s) 74, 122, 125
HpyAV CCTTC 3 cut(s) 210, 295, 430
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 3 cut(s) 91, 313, 328
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 23, 88, 269, 437
HpyF3I CTNAG 3 cut(s) 143, 177, 289
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 2 cut(s) 120, 150
Hsp92II CATG 2 cut(s) 57, 172
HspAI GCGC 2 cut(s) 212, 361
Kzo9I GATC 1 cut(s) 448
LpnPI CCDG 7 cut(s) 101, 148, 237, 253, 338, 339, 454
Lsp1109I GCAGC 4 cut(s) 17, 20, 103, 168
LweI GCATC 1 cut(s) 20
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 1 cut(s) 130
MalI GATC 1 cut(s) 450
MbiI CCGCTC 1 cut(s) 147
MboI GATC 1 cut(s) 448
MboII GAAGA 5 cut(s) 25, 268, 409, 421, 443
MluCI AATT 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 3 cut(s) 100, 215, 330
MspA1I CMGCKG 2 cut(s) 142, 181
MspI CCGG 1 cut(s) 441
MspR9I CCNGG 1 cut(s) 442
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 5 cut(s) 14, 23, 88, 269, 437
NciI CCSGG 1 cut(s) 442
NdeII GATC 1 cut(s) 448
NlaIII CATG 2 cut(s) 57, 172
NlaIV GGNNCC 2 cut(s) 40, 86
NspI RCATGY 1 cut(s) 172
PciI ACATGT 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 1 cut(s) 156
PkrI GCNGC 6 cut(s) 7, 10, 25, 93, 149, 183
PscI ACATGT 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 353
PspN4I GGNNCC 2 cut(s) 40, 86
PspPI GGNCC 1 cut(s) 66
PsrI GAACNNNNNNTAC 2 cut(s) 314, 346
PstI CTGCAG 2 cut(s) 93, 330
PvuII CAGCTG 2 cut(s) 142, 181
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SatI GCNGC 6 cut(s) 6, 9, 24, 92, 148, 182
Sau3AI GATC 1 cut(s) 448
Sau96I GGNCC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 442
SetI ASST 5 cut(s) 123, 144, 183, 327, 355
SfaNI GCATC 1 cut(s) 20
SfcI CTRYAG 2 cut(s) 89, 326
Sse9I AATT 1 cut(s) 237
SsiI CCGC 6 cut(s) 24, 82, 104, 147, 210, 376
StyD4I CCNGG 1 cut(s) 440
TaiI ACGT 1 cut(s) 123
TasI AATT 1 cut(s) 237
TatI WGTACW 1 cut(s) 330
TauI GCSGC 2 cut(s) 26, 150
TfiI GAWTC 1 cut(s) 156
TscAI CASTG 1 cut(s) 256
TseI GCWGC 4 cut(s) 5, 8, 91, 181
TspRI CASTG 1 cut(s) 256
XapI RAATTY 1 cut(s) 237
XceI RCATGY 1 cut(s) 172
ZraI GACGTC 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.