Rw4G004320

50S ribosomal protein L28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009333
Physical Location & Seq
Forward (+)
8617778 .. 8619977
2200 bp
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UTR
Exon/CDS
Intron
Rw4G004320.1

Sequence Viewer

Length: 474 bp
ATGGCAGCTTCCGCAACCGCGGCTGTGTTCTCCGGTCTATGCTTCCGCCGAGGAGCAGAGTCCTACAAGAGGTTGTCTTCCAAGACCCGAGCAGTTTCCGATATTGGGTTCGTCACCAGTCAGCTCAGTGGCCTCAAAATCTCCTACGACGTGTCGTCTCAGCTCCCAACTCCCATCTTCACCCCTTCCCTCCCTGGCCTTCAGCCCATTGTTGCTCGTAGAATTTGTCCTTTTACTGGGAAGAAAGCAAACAAGGCAAACAAGATTTCCTTCTCGGCCCACAAGACCAAGAAGTTGCAGTTTGTGAACCTGCAGTACAAGAGGATTTGGTGGGAAGGTGGGAAGCGCTTTTTGAAGCTGCGCTTGTCGACCAAAGCAATCAAGACCATAGAGAAGAATGGACTGGAAGCCGTTGCCAAGAAGGCTGGCATAGATCTTCCATGTTGTGCAAAGAATGGATCTGATAACACTTGA

Protein Analysis

157

Amino Acids

17.14

Weight (kDa)

10.33

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28 PF00830 74 - 133 9.7e-19 Ribosomal L28 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 318
AccI GTMKAC 1 cut(s) 368
AccII CGCG 1 cut(s) 20
AciI CCGC 4 cut(s) 12, 18, 20, 46
AclWI GGATC 1 cut(s) 466
AcsI RAATTY 1 cut(s) 222
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 1 cut(s) 317
AfeI AGCGCT 1 cut(s) 347
AfiI CCNNNNNNNGG 3 cut(s) 69, 105, 236
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 1 cut(s) 355
AhdI GACNNNNNGTC 1 cut(s) 154
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 193
AluBI AGCT 4 cut(s) 8, 124, 163, 358
AluI AGCT 4 cut(s) 8, 124, 163, 358
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 1 cut(s) 466
Ama87I CYCGRG 1 cut(s) 87
Aor51HI AGCGCT 1 cut(s) 347
AoxI GGCC 3 cut(s) 130, 196, 276
ApeKI GCWGC 2 cut(s) 5, 358
ApoI RAATTY 1 cut(s) 222
AspLEI GCGC 2 cut(s) 348, 363
AspS9I GGNCC 1 cut(s) 277
AsuHPI GGTGA 2 cut(s) 106, 172
AvaI CYCGRG 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 17, 345
BccI CCATC 1 cut(s) 182
BceAI ACGGC 1 cut(s) 395
BciT130I CCWGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 311
BfoI RGCGCY 1 cut(s) 349
BfuAI ACCTGC 1 cut(s) 318
BglI GCCNNNNNGGC 1 cut(s) 422
BglII AGATCT 1 cut(s) 433
BisI GCNGC 3 cut(s) 6, 21, 359
BlsI GCNGC 3 cut(s) 7, 22, 360
Bme1390I CCNGG 1 cut(s) 195
BmeRI GACNNNNNGTC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 87
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 277
BmrFI CCNGG 1 cut(s) 195
BmrI ACTGGG 1 cut(s) 246
BmuI ACTGGG 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 69
BsaJI CCNNGG 3 cut(s) 18, 49, 193
BsaWI WCCGGW 1 cut(s) 32
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 105, 236
Bse1I ACTGG 3 cut(s) 117, 241, 408
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 3 cut(s) 18, 49, 193
BseLI CCNNNNNNNGG 3 cut(s) 69, 105, 236
BseMII CTCAG 2 cut(s) 139, 173
BseNI ACTGG 3 cut(s) 117, 241, 408
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 17, 345
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 3 cut(s) 132, 198, 278
BsiHKCI CYCGRG 1 cut(s) 87
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 3 cut(s) 69, 105, 236
BsmAI GTCTC 1 cut(s) 162
BsmBI CGTCTC 1 cut(s) 162
BsnI GGCC 3 cut(s) 132, 198, 278
BsoBI CYCGRG 1 cut(s) 87
Bsp143I GATC 2 cut(s) 433, 458
BspACI CCGC 4 cut(s) 12, 18, 20, 46
BspANI GGCC 3 cut(s) 132, 198, 278
BspCNI CTCAG 2 cut(s) 138, 172
BspFNI CGCG 1 cut(s) 20
BspMAI CTGCAG 1 cut(s) 315
BspMI ACCTGC 1 cut(s) 318
BspPI GGATC 1 cut(s) 466
BsrI ACTGG 3 cut(s) 117, 241, 408
BssECI CCNNGG 3 cut(s) 18, 49, 193
BssMI GATC 2 cut(s) 433, 458
Bst2UI CCWGG 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 2 cut(s) 125, 159
BstDSI CCRYGG 1 cut(s) 18
BstENI CCTNNNNNAGG 1 cut(s) 67
BstFNI CGCG 1 cut(s) 20
BstH2I RGCGCY 1 cut(s) 349
BstHHI GCGC 2 cut(s) 348, 363
BstKTI GATC 2 cut(s) 436, 461
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 2 cut(s) 433, 458
BstMWI GCNNNNNNNGC 4 cut(s) 11, 20, 254, 422
BstNI CCWGG 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 311
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 17, 345
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 2 cut(s) 433, 458
BstYI RGATCY 2 cut(s) 433, 458
BsuRI GGCC 3 cut(s) 132, 198, 278
BtgI CCRYGG 1 cut(s) 18
BtrI CACGTC 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 133
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 1 cut(s) 427
CfoI GCGC 2 cut(s) 348, 363
Cfr13I GGNCC 1 cut(s) 277
Cfr42I CCGCGG 1 cut(s) 21
Csp6I GTAC 1 cut(s) 316
CviAII CATG 1 cut(s) 441
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 2 cut(s) 125, 159
DpnI GATC 2 cut(s) 435, 460
DpnII GATC 2 cut(s) 433, 458
DriI GACNNNNNGTC 1 cut(s) 154
Eam1105I GACNNNNNGTC 1 cut(s) 154
EciI GGCGGA 1 cut(s) 35
Eco47III AGCGCT 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 185
Eco88I CYCGRG 1 cut(s) 87
EcoNI CCTNNNNNAGG 1 cut(s) 67
EcoRII CCWGG 1 cut(s) 193
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 1 cut(s) 444
FaiI YATR 4 cut(s) 40, 389, 431, 442
FalI AAGNNNNNCTT 4 cut(s) 254, 286, 347, 379
FatI CATG 1 cut(s) 440
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 3 cut(s) 6, 21, 359
Fsp4HI GCNGC 3 cut(s) 6, 21, 359
GlaI GCGC 2 cut(s) 347, 362
GluI GCNGC 3 cut(s) 6, 21, 359
HaeII RGCGCY 1 cut(s) 349
HaeIII GGCC 3 cut(s) 132, 198, 278
HapII CCGG 1 cut(s) 33
HhaI GCGC 2 cut(s) 348, 363
Hin1II CATG 1 cut(s) 444
Hin6I GCGC 2 cut(s) 346, 361
HinP1I GCGC 2 cut(s) 346, 361
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HinfI GANTC 1 cut(s) 59
HpaII CCGG 1 cut(s) 33
HphI GGTGA 2 cut(s) 106, 172
Hpy166II GTNNAC 2 cut(s) 307, 369
Hpy188I TCNGA 2 cut(s) 100, 463
Hpy188III TCNNGA 1 cut(s) 382
Hpy8I GTNNAC 2 cut(s) 307, 369
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 195, 209, 280, 329, 415
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 3 cut(s) 298, 313, 449
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 20, 254, 422
HpyF3I CTNAG 2 cut(s) 125, 159
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 1 cut(s) 444
HspAI GCGC 2 cut(s) 346, 361
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 2 cut(s) 433, 458
LmnI GCTCC 2 cut(s) 53, 168
LpnPI CCDG 8 cut(s) 46, 130, 180, 207, 222, 323, 389, 411
Lsp1109I GCAGC 2 cut(s) 17, 345
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 112
MalI GATC 2 cut(s) 435, 460
MboI GATC 2 cut(s) 433, 458
MboII GAAGA 5 cut(s) 69, 169, 253, 406, 428
MflI RGATCY 2 cut(s) 433, 458
MluCI AATT 1 cut(s) 222
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 5 cut(s) 44, 63, 143, 200, 315
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 195
MvaI CCWGG 1 cut(s) 195
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 4 cut(s) 11, 20, 254, 422
NdeII GATC 2 cut(s) 433, 458
NlaIII CATG 1 cut(s) 444
NmeAIII GCCGAG 2 cut(s) 74, 254
NmuCI GTSAC 1 cut(s) 112
PkrI GCNGC 3 cut(s) 7, 22, 360
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspPI GGNCC 1 cut(s) 277
PsrI GAACNNNNNNTAC 2 cut(s) 299, 331
PstI CTGCAG 1 cut(s) 315
PsuI RGATCY 2 cut(s) 433, 458
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SacII CCGCGG 1 cut(s) 21
SalI GTCGAC 1 cut(s) 367
SatI GCNGC 3 cut(s) 6, 21, 359
Sau3AI GATC 2 cut(s) 433, 458
Sau96I GGNCC 1 cut(s) 277
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 195
SetI ASST 8 cut(s) 10, 74, 126, 153, 165, 312, 340, 360
SfcI CTRYAG 1 cut(s) 311
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
Sse9I AATT 1 cut(s) 222
SsiI CCGC 4 cut(s) 12, 18, 20, 46
StyD4I CCNGG 1 cut(s) 193
TaiI ACGT 1 cut(s) 153
TaqI TCGA 1 cut(s) 368
TasI AATT 1 cut(s) 222
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 23
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 2 cut(s) 5, 358
Tsp45I GTSAC 1 cut(s) 112
TspRI CASTG 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 67
XapI RAATTY 1 cut(s) 222
XmiI GTMKAC 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.