RchiOBHm_Chr4g0394651

50S ribosomal protein L28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009333
Physical Location & Seq
Forward (+)
10334444 .. 10335796
1353 bp
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UTR
Exon/CDS
Intron
PRQ36712

Sequence Viewer

Length: 462 bp
ATGGCAGCTTCCGCAACCGCGGCTATCTTCTTCGGCAGCAGTTCCGGTCTATGCCTCCTCCGCGGAGCAGAGTCCTCCAAGAGAATATTGTCTTCCATGACCCAAGCAGTTTCCGATATTGGGTTCCTCACCAATCAGCTCAGTGGCCTCAAAATCTCCTATGACGTGTCGTCTCAGCTCCCCAAGTCCCGTCTTCACTCCTTCCCTCCCTGGCTTTCAGCGCATTGTTGCTCAATTTGTCCTTTTACTGGGAAGAAAGCAAACTACGCAAACAAGATTTCCTTCTCGGCCCACAAGACCAAGAAGTTGCAGTTTGTGAACCTGCCGTACAAGAGGATTTGGTGGGAAGGCGGGAAGCGCTTTTTGAAGCTGCGCTTGTCGGCCAGAGCAATCAAGACCATAGAGGAAAATGGACTGGAAGCCGTTGCCAAGAAGGCCGGGATAGATCTACGCAAGTGTTGA

Protein Analysis

153

Amino Acids

16.8

Weight (kDa)

10.07

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28 PF00830 80 - 135 1.3e-16 Ribosomal L28 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 330
AccII CGCG 2 cut(s) 20, 63
AciI CCGC 6 cut(s) 12, 18, 20, 61, 63, 351
AcoI YGGCCR 1 cut(s) 381
AfaI GTAC 1 cut(s) 329
AfeI AGCGCT 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 120, 248
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 1 cut(s) 367
AhdI GACNNNNNGTC 1 cut(s) 169
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 1 cut(s) 209
AluBI AGCT 4 cut(s) 8, 139, 178, 370
AluI AGCT 4 cut(s) 8, 139, 178, 370
Alw26I GTCTC 1 cut(s) 177
Aor51HI AGCGCT 1 cut(s) 359
AoxI GGCC 4 cut(s) 145, 288, 381, 435
ApeKI GCWGC 3 cut(s) 5, 36, 370
AspLEI GCGC 3 cut(s) 223, 360, 375
AspS9I GGNCC 1 cut(s) 289
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 1 cut(s) 121
BbsI GAAGAC 2 cut(s) 84, 185
BbvI GCAGC 3 cut(s) 17, 48, 357
BceAI ACGGC 2 cut(s) 310, 407
BciT130I CCWGG 1 cut(s) 211
BcnI CCSGG 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 177
BfoI RGCGCY 1 cut(s) 361
BfuAI ACCTGC 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 434
BglII AGATCT 1 cut(s) 445
BisI GCNGC 4 cut(s) 6, 21, 37, 371
BlsI GCNGC 4 cut(s) 7, 22, 38, 372
Bme1390I CCNGG 2 cut(s) 211, 439
BmeRI GACNNNNNGTC 1 cut(s) 169
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 125
BmrFI CCNGG 2 cut(s) 211, 439
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
BpiI GAAGAC 2 cut(s) 84, 185
BpuMI CCSGG 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 18, 61, 209
BsaWI WCCGGW 1 cut(s) 44
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 248
Bse1I ACTGG 2 cut(s) 253, 420
BseBI CCWGG 1 cut(s) 211
BseDI CCNNGG 3 cut(s) 18, 61, 209
BseLI CCNNNNNNNGG 2 cut(s) 120, 248
BseMII CTCAG 2 cut(s) 154, 188
BseNI ACTGG 2 cut(s) 253, 420
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 3 cut(s) 17, 48, 357
Bsh1236I CGCG 2 cut(s) 20, 63
BshFI GGCC 4 cut(s) 147, 290, 383, 437
BsiSI CCGG 2 cut(s) 45, 438
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 2 cut(s) 120, 248
BsmAI GTCTC 1 cut(s) 177
BsmBI CGTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 4 cut(s) 147, 290, 383, 437
Bsp143I GATC 1 cut(s) 445
BspACI CCGC 6 cut(s) 12, 18, 20, 61, 63, 351
BspANI GGCC 4 cut(s) 147, 290, 383, 437
BspCNI CTCAG 2 cut(s) 153, 187
BspFNI CGCG 2 cut(s) 20, 63
BspLI GGNNCC 1 cut(s) 125
BspMI ACCTGC 1 cut(s) 330
BsrI ACTGG 2 cut(s) 253, 420
BssECI CCNNGG 3 cut(s) 18, 61, 209
BssMI GATC 1 cut(s) 445
Bst2UI CCWGG 1 cut(s) 211
BstDEI CTNAG 2 cut(s) 140, 174
BstDSI CCRYGG 2 cut(s) 18, 61
BstFNI CGCG 2 cut(s) 20, 63
BstH2I RGCGCY 1 cut(s) 361
BstHHI GCGC 3 cut(s) 223, 360, 375
BstKTI GATC 1 cut(s) 448
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 1 cut(s) 445
BstMWI GCNNNNNNNGC 7 cut(s) 11, 20, 60, 220, 266, 357, 434
BstNI CCWGG 1 cut(s) 211
BstSCI CCNGG 2 cut(s) 209, 437
BstUI CGCG 2 cut(s) 20, 63
BstV1I GCAGC 3 cut(s) 17, 48, 357
BstV2I GAAGAC 2 cut(s) 84, 185
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
BsuRI GGCC 4 cut(s) 147, 290, 383, 437
BtgI CCRYGG 2 cut(s) 18, 61
BtrI CACGTC 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 148
BveI ACCTGC 1 cut(s) 330
CfoI GCGC 3 cut(s) 223, 360, 375
Cfr13I GGNCC 1 cut(s) 289
Cfr42I CCGCGG 2 cut(s) 21, 64
Csp6I GTAC 1 cut(s) 328
CviAII CATG 1 cut(s) 97
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 2 cut(s) 140, 174
DpnI GATC 1 cut(s) 447
DpnII GATC 1 cut(s) 445
DriI GACNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 381
Eam1105I GACNNNNNGTC 1 cut(s) 169
Eco47III AGCGCT 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 209
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 1 cut(s) 100
FaiI YATR 4 cut(s) 52, 98, 162, 401
FalI AAGNNNNNCTT 4 cut(s) 266, 298, 359, 391
FaqI GGGAC 1 cut(s) 172
FatI CATG 1 cut(s) 96
FauI CCCGC 1 cut(s) 344
Fnu4HI GCNGC 4 cut(s) 6, 21, 37, 371
Fsp4HI GCNGC 4 cut(s) 6, 21, 37, 371
GlaI GCGC 3 cut(s) 222, 359, 374
GluI GCNGC 4 cut(s) 6, 21, 37, 371
HaeII RGCGCY 1 cut(s) 361
HaeIII GGCC 4 cut(s) 147, 290, 383, 437
HapII CCGG 2 cut(s) 45, 438
HhaI GCGC 3 cut(s) 223, 360, 375
Hin1II CATG 1 cut(s) 100
Hin6I GCGC 3 cut(s) 221, 358, 373
HinP1I GCGC 3 cut(s) 221, 358, 373
HinfI GANTC 1 cut(s) 71
HpaII CCGG 2 cut(s) 45, 438
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 1 cut(s) 394
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 4 cut(s) 211, 292, 341, 427
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 1 cut(s) 310
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 20, 60, 220, 266, 357, 434
HpyF3I CTNAG 2 cut(s) 140, 174
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 1 cut(s) 100
HspAI GCGC 3 cut(s) 221, 358, 373
KspI CCGCGG 2 cut(s) 21, 64
Kzo9I GATC 1 cut(s) 445
LmnI GCTCC 2 cut(s) 65, 183
LpnPI CCDG 8 cut(s) 58, 196, 223, 234, 335, 397, 401, 451
Lsp1109I GCAGC 3 cut(s) 17, 48, 357
MaeII ACGT 1 cut(s) 165
MalI GATC 1 cut(s) 447
MboI GATC 1 cut(s) 445
MboII GAAGA 5 cut(s) 19, 22, 84, 185, 265
MflI RGATCY 1 cut(s) 445
MluCI AATT 1 cut(s) 234
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 8 cut(s) 65, 68, 85, 137, 158, 216, 327, 397
MspA1I CMGCKG 2 cut(s) 20, 63
MspI CCGG 2 cut(s) 45, 438
MspR9I CCNGG 2 cut(s) 211, 439
MvaI CCWGG 1 cut(s) 211
MvnI CGCG 2 cut(s) 20, 63
MwoI GCNNNNNNNGC 7 cut(s) 11, 20, 60, 220, 266, 357, 434
NciI CCSGG 1 cut(s) 439
NdeII GATC 1 cut(s) 445
NlaIII CATG 1 cut(s) 100
NlaIV GGNNCC 1 cut(s) 125
NmeAIII GCCGAG 1 cut(s) 266
PkrI GCNGC 4 cut(s) 7, 22, 38, 372
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 209
PspGI CCWGG 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 125
PspPI GGNCC 1 cut(s) 289
PsrI GAACNNNNNNTAC 2 cut(s) 311, 343
PsuI RGATCY 1 cut(s) 445
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SacII CCGCGG 2 cut(s) 21, 64
SatI GCNGC 4 cut(s) 6, 21, 37, 371
Sau3AI GATC 1 cut(s) 445
Sau96I GGNCC 1 cut(s) 289
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 2 cut(s) 211, 439
SetI ASST 6 cut(s) 10, 141, 168, 180, 324, 372
Sfr303I CCGCGG 2 cut(s) 21, 64
SgrBI CCGCGG 2 cut(s) 21, 64
Sse9I AATT 1 cut(s) 234
SsiI CCGC 6 cut(s) 12, 18, 20, 61, 63, 351
SspI AATATT 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 209, 437
TaiI ACGT 1 cut(s) 168
TasI AATT 1 cut(s) 234
TauI GCSGC 1 cut(s) 23
TscAI CASTG 1 cut(s) 148
TseI GCWGC 3 cut(s) 5, 36, 370
TspRI CASTG 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.