FvH4_4g04610

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009760
Physical Location & Seq
Reverse (-)
4016509 .. 4017185
677 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04610.t1

Sequence Viewer

Length: 330 bp
ATGCACAATGGAGGCAGCTTTGATTATCTTTCTACTCCCCGGGAGACAGAAAATGCAGCCAAGATGCTTACTTTCCAACTTGGCATTGCACCAGTTTACATTTCATTTCTCCATTTCATGGGCAATGCGGTCGAAGCACGAACCTTTAGCTACAGACTGGTAGTTGGAGCACATGGGAGGAAACTCATATGGGAAGGAACCGCTCGGAGCATACGCGAGAACTATCAAAAGGTAAGGGATAGCCATGACGGTCTCATAATCCAGCGCAACATGGCACTCTTCTTCTCCACTGGAGAAAGGAAGGAGCTCAAGCTTCGGGTCACCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04115 ko04120 ko04310 map04013 map04115 map04120 map04310
Pfam Domains
Protein Families
Sina

Protein Analysis

110

Amino Acids

12.52

Weight (kDa)

10.07

Isoelectric Point (pI)

23.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sina_TRAF PF03145 28 - 108 7.3e-23 Sina, TRAF-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 203
AccII CGCG 1 cut(s) 216
AciI CCGC 2 cut(s) 128, 201
AfiI CCNNNNNNNGG 1 cut(s) 118
AluBI AGCT 4 cut(s) 18, 150, 307, 313
AluI AGCT 4 cut(s) 18, 150, 307, 313
Alw21I GWGCWC 2 cut(s) 172, 309
Alw26I GTCTC 2 cut(s) 38, 257
Ama87I CYCGRG 1 cut(s) 39
ApeKI GCWGC 2 cut(s) 15, 56
AspLEI GCGC 1 cut(s) 267
AsuC2I CCSGG 2 cut(s) 40, 41
AsuHPI GGTGA 1 cut(s) 313
AvaI CYCGRG 1 cut(s) 39
BanII GRGCYC 1 cut(s) 309
Bbv12I GWGCWC 2 cut(s) 172, 309
BbvI GCAGC 2 cut(s) 27, 68
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BcnI CCSGG 2 cut(s) 40, 41
BcoDI GTCTC 2 cut(s) 38, 257
BfmI CTRYAG 1 cut(s) 151
BisI GCNGC 2 cut(s) 16, 57
BlsI GCNGC 2 cut(s) 17, 58
Bme1390I CCNGG 2 cut(s) 40, 41
BmeT110I CYCGRG 1 cut(s) 39
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 2 cut(s) 40, 41
BmsI GCATC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 2 cut(s) 40, 41
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 38, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 3 cut(s) 92, 162, 295
Bse3DI GCAATG 2 cut(s) 84, 130
BseDI CCNNGG 2 cut(s) 38, 39
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 2 cut(s) 84, 130
BseNI ACTGG 3 cut(s) 92, 162, 295
BseXI GCAGC 2 cut(s) 27, 68
Bsh1236I CGCG 1 cut(s) 216
Bsh1285I CGRYCG 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 172, 309
BsiHKCI CYCGRG 1 cut(s) 39
BsiSI CCGG 1 cut(s) 40
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 2 cut(s) 38, 257
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 172, 309
BspACI CCGC 2 cut(s) 128, 201
BspFNI CGCG 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 199
BspTNI GGTCTC 1 cut(s) 257
BsrBI CCGCTC 1 cut(s) 203
BsrDI GCAATG 2 cut(s) 84, 130
BsrI ACTGG 3 cut(s) 92, 162, 295
BssECI CCNNGG 2 cut(s) 38, 39
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 1 cut(s) 284
BstEII GGTNACC 1 cut(s) 319
BstFNI CGCG 1 cut(s) 216
BstHHI GCGC 1 cut(s) 267
BstMAI GTCTC 2 cut(s) 38, 257
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstPI GGTNACC 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 38, 39
BstSFI CTRYAG 1 cut(s) 151
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 2 cut(s) 27, 68
BtsIMutI CAGTG 1 cut(s) 288
CfoI GCGC 1 cut(s) 267
Cfr9I CCCGGG 1 cut(s) 39
CviAII CATG 4 cut(s) 118, 173, 245, 271
CviJI RGCY 6 cut(s) 18, 59, 150, 243, 307, 313
CviKI_1 RGCY 6 cut(s) 18, 59, 150, 243, 307, 313
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Ecl136II GAGCTC 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 257
Eco53kI GAGCTC 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 39
Eco91I GGTNACC 1 cut(s) 319
EcoICRI GAGCTC 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 309
FaeI CATG 4 cut(s) 121, 176, 248, 274
FaiI YATR 8 cut(s) 119, 174, 188, 190, 212, 246, 257, 272
FatI CATG 4 cut(s) 117, 172, 244, 270
FauNDI CATATG 1 cut(s) 188
Fnu4HI GCNGC 2 cut(s) 16, 57
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 2 cut(s) 16, 57
GlaI GCGC 1 cut(s) 266
GluI GCNGC 2 cut(s) 16, 57
GsuI CTGGAG 1 cut(s) 312
HapII CCGG 1 cut(s) 40
HhaI GCGC 1 cut(s) 267
Hin1II CATG 4 cut(s) 121, 176, 248, 274
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 311
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 1 cut(s) 207
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 2 cut(s) 188, 295
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4V TGCA 3 cut(s) 4, 56, 89
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
Hsp92II CATG 4 cut(s) 121, 176, 248, 274
HspAI GCGC 1 cut(s) 265
LmnI GCTCC 3 cut(s) 167, 207, 304
LpnPI CCDG 5 cut(s) 53, 105, 143, 275, 276
Lsp1109I GCAGC 2 cut(s) 27, 68
LweI GCATC 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 319
MbiI CCGCTC 1 cut(s) 203
MboII GAAGA 2 cut(s) 271, 274
MhlI GDGCHC 2 cut(s) 172, 309
MmeI TCCRAC 2 cut(s) 100, 145
MnlI CCTC 2 cut(s) 5, 171
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 40, 41
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 134
NciI CCSGG 2 cut(s) 40, 41
NdeI CATATG 1 cut(s) 188
NlaIII CATG 4 cut(s) 121, 176, 248, 274
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 319
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 2 cut(s) 17, 58
Psp124BI GAGCTC 1 cut(s) 309
PspEI GGTNACC 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 199
SacI GAGCTC 1 cut(s) 309
SatI GCNGC 2 cut(s) 16, 57
ScrFI CCNGG 2 cut(s) 40, 41
SduI GDGCHC 2 cut(s) 172, 309
SetI ASST 6 cut(s) 20, 146, 152, 234, 309, 315
SfaNI GCATC 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 151
SmaI CCCGGG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SsiI CCGC 2 cut(s) 128, 201
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 2 cut(s) 38, 39
TaaI ACNGT 1 cut(s) 251
TaqI TCGA 1 cut(s) 132
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 2 cut(s) 15, 56
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 2 cut(s) 93, 106
TspMI CCCGGG 1 cut(s) 39
TspRI CASTG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 118
XmaI CCCGGG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.