FvH4_4g06651

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003467
Physical Location & Seq
Reverse (-)
5938179 .. 5943240
5062 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06651.t4

Sequence Viewer

Length: 804 bp
ATGCAGTCTTCTCCGGTTATGGCTACCAAATTCCATTGCACAGCACCCGTCGTTGTCTATAAGCAGAGATCCACCTTGTCCCGGAGCCCCGAGAACTTCAAATTCAAATGGGACTTCACAAGGTGCATCAATATCTCATGGAGATGGGTTTCTCAGATGATCTCATTTTACTTGTTCTTTCTCAATATTGTTGCTGATGCTCAGGCCTACGATTACCCATCTCCTGTTTATGCTTGTGAAGATGTTAACAATTACTATGCCAATGTGGAGCATCTAGAGGGTAAATTACTCAAGAAGAGACTGAATTCCATCATTGGCAAGCATCAATCTTTATCTTACAGAGAGGTATGGAATGCTCTCAAGATTCTAGATGCTTCGGATGTTGACAACCCAGAAGCTTCATCAGGGATAGTTGAGATTTACTCCCTGAAGGTTGTACCAAAGTCATTAGCTGGAAAGCCTGAAGGATGGAATAGAGAGCACTTATGGCCCCGTTCTTATGGTTTGAAAAATGGTCCATCCCTGACTGATATACACAACATTCGCCCGGCAGATGTCAATGTCAATTCCTCTAGGGGGAATAAATACTATGGAGAATGCGGTGTTACTTCAGCTAAATGTTTGAAGCCAGCAAATATAGAAGCTGCTTTGGACACAGAAACTGACAAGGATAGATGGGCGCCACCTAAACAGAGGAGATATTGCAAGGGCACTGATGTACATGGCAGTATGTTATGGTTATCGTCAATCAGATGGAGGCCCAGTTCTTCACCTTTCAGATTCTCCAAAGATTTCAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

268

Amino Acids

30.62

Weight (kDa)

9.25

Isoelectric Point (pI)

66.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 126 - 238 4e-16 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 679
AciI CCGC 1 cut(s) 600
AclWI GGATC 1 cut(s) 63
AcsI RAATTY 3 cut(s) 29, 101, 304
AcuI CTGAAG 3 cut(s) 449, 483, 594
AcyI GRCGYC 1 cut(s) 680
AdeI CACNNNGTG 1 cut(s) 123
AfaI GTAC 2 cut(s) 438, 720
AfiI CCNNNNNNNGG 2 cut(s) 81, 576
AgsI TTSAA 5 cut(s) 100, 106, 508, 625, 796
AloI GAACNNNNNNTCC 2 cut(s) 748, 780
AluBI AGCT 4 cut(s) 398, 452, 614, 644
AluI AGCT 4 cut(s) 398, 452, 614, 644
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 1 cut(s) 292
AlwI GGATC 1 cut(s) 63
AlwNI CAGNNNCTG 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 3 cut(s) 204, 488, 758
ApeKI GCWGC 1 cut(s) 644
ApoI RAATTY 3 cut(s) 29, 101, 304
AspLEI GCGC 1 cut(s) 682
AspS9I GGNCC 3 cut(s) 489, 515, 759
AsuC2I CCSGG 2 cut(s) 82, 548
AsuHPI GGTGA 1 cut(s) 762
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 1 cut(s) 515
BaeGI GKGCMC 1 cut(s) 713
BanI GGYRCC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 1 cut(s) 631
BccI CCATC 7 cut(s) 138, 226, 317, 462, 526, 669, 747
BcnI CCSGG 2 cut(s) 82, 548
BcoDI GTCTC 1 cut(s) 292
BfaI CTAG 3 cut(s) 275, 368, 573
BfoI RGCGCY 1 cut(s) 683
BisI GCNGC 1 cut(s) 645
BlsI GCNGC 1 cut(s) 646
Bme1390I CCNGG 2 cut(s) 82, 548
Bme18I GGWCC 1 cut(s) 515
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 3 cut(s) 489, 515, 759
BmiI GGNNCC 3 cut(s) 86, 491, 681
BmrFI CCNGG 2 cut(s) 82, 548
BmrI ACTGGG 1 cut(s) 756
BmsI GCATC 5 cut(s) 135, 187, 280, 331, 361
BmuI ACTGGG 1 cut(s) 756
BplI GAGNNNNNCTC 2 cut(s) 407, 439
Bpu10I CCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 275, 344
BpuMI CCSGG 2 cut(s) 82, 548
BsaHI GRCGYC 1 cut(s) 680
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 748, 778
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 576
Bse1I ACTGG 1 cut(s) 762
Bse3DI GCAATG 1 cut(s) 34
BseGI GGATG 3 cut(s) 385, 473, 518
BseLI CCNNNNNNNGG 2 cut(s) 81, 576
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 2 cut(s) 167, 215
BseNI ACTGG 1 cut(s) 762
BseRI GAGGAG 1 cut(s) 709
BseSI GKGCMC 1 cut(s) 713
BseXI GCAGC 1 cut(s) 631
BshFI GGCC 3 cut(s) 206, 490, 760
BshNI GGYRCC 1 cut(s) 679
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 3 cut(s) 14, 82, 548
BslFI GGGAC 2 cut(s) 64, 125
BslI CCNNNNNNNGG 2 cut(s) 81, 576
BsmAI GTCTC 1 cut(s) 292
BsmFI GGGAC 2 cut(s) 64, 125
BsmI GAATGC 2 cut(s) 358, 602
BsnI GGCC 3 cut(s) 206, 490, 760
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 3 cut(s) 89, 483, 713
Bsp1407I TGTACA 1 cut(s) 718
Bsp143I GATC 2 cut(s) 68, 159
BspACI CCGC 1 cut(s) 600
BspANI GGCC 3 cut(s) 206, 490, 760
BspCNI CTCAG 2 cut(s) 166, 214
BspLI GGNNCC 3 cut(s) 86, 491, 681
BspPI GGATC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 679
BsrDI GCAATG 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 718
BsrI ACTGG 1 cut(s) 762
BssMI GATC 2 cut(s) 68, 159
BssNI GRCGYC 1 cut(s) 680
Bst6I CTCTTC 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 680
BstAUI TGTACA 1 cut(s) 718
BstC8I GCNNGC 2 cut(s) 320, 630
BstDEI CTNAG 2 cut(s) 153, 201
BstF5I GGATG 3 cut(s) 385, 473, 518
BstH2I RGCGCY 1 cut(s) 683
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 2 cut(s) 71, 162
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 2 cut(s) 68, 159
BstMWI GCNNNNNNNGC 1 cut(s) 487
BstSCI CCNGG 2 cut(s) 80, 546
BstSLI GKGCMC 1 cut(s) 713
BstV1I GCAGC 1 cut(s) 631
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 3 cut(s) 206, 490, 760
BtsCI GGATG 3 cut(s) 385, 473, 518
BtsIMutI CAGTG 1 cut(s) 711
Cac8I GCNNGC 2 cut(s) 320, 630
CaiI CAGNNNCTG 1 cut(s) 662
CfoI GCGC 1 cut(s) 682
Cfr13I GGNCC 3 cut(s) 489, 515, 759
Csp6I GTAC 2 cut(s) 437, 719
CviAII CATG 2 cut(s) 138, 722
CviQI GTAC 2 cut(s) 437, 719
DdeI CTNAG 2 cut(s) 153, 201
DinI GGCGCC 1 cut(s) 681
DpnI GATC 2 cut(s) 70, 161
DpnII GATC 2 cut(s) 68, 159
DraIII CACNNNGTG 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Eco147I AGGCCT 1 cut(s) 206
Eco24I GRGCYC 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 515
Eco57I CTGAAG 3 cut(s) 449, 483, 594
Eco88I CYCGRG 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 89
EgeI GGCGCC 1 cut(s) 681
EheI GGCGCC 1 cut(s) 681
FaeI CATG 2 cut(s) 141, 725
FaqI GGGAC 2 cut(s) 64, 125
FatI CATG 2 cut(s) 137, 721
Fnu4HI GCNGC 1 cut(s) 645
FokI GGATG 3 cut(s) 392, 480, 505
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 645
FspBI CTAG 3 cut(s) 275, 368, 573
GlaI GCGC 1 cut(s) 681
GluI GCNGC 1 cut(s) 645
HaeII RGCGCY 1 cut(s) 683
HaeIII GGCC 3 cut(s) 206, 490, 760
HapII CCGG 3 cut(s) 14, 82, 548
HhaI GCGC 1 cut(s) 682
Hin1I GRCGYC 1 cut(s) 680
Hin1II CATG 2 cut(s) 141, 725
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HincII GTYRAC 2 cut(s) 247, 385
HindII GTYRAC 2 cut(s) 247, 385
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 2 cut(s) 364, 780
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 3 cut(s) 14, 82, 548
HphI GGTGA 1 cut(s) 762
Hpy166II GTNNAC 2 cut(s) 247, 385
Hpy188I TCNGA 4 cut(s) 156, 379, 752, 779
Hpy188III TCNNGA 4 cut(s) 275, 292, 361, 368
Hpy8I GTNNAC 2 cut(s) 247, 385
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 424, 458
HpyCH4V TGCA 4 cut(s) 4, 39, 126, 705
HpyF10VI GCNNNNNNNGC 1 cut(s) 487
HpyF3I CTNAG 2 cut(s) 153, 201
Hsp92I GRCGYC 1 cut(s) 680
Hsp92II CATG 2 cut(s) 141, 725
HspAI GCGC 1 cut(s) 680
KasI GGCGCC 1 cut(s) 679
KspAI GTTAAC 1 cut(s) 247
Kzo9I GATC 2 cut(s) 68, 159
LmnI GCTCC 2 cut(s) 84, 268
Lsp1109I GCAGC 1 cut(s) 631
LweI GCATC 5 cut(s) 135, 187, 280, 331, 361
MaeI CTAG 3 cut(s) 275, 368, 573
MaeIII GTNAC 1 cut(s) 604
MalI GATC 2 cut(s) 70, 161
MboI GATC 2 cut(s) 68, 159
MboII GAAGA 3 cut(s) 251, 307, 759
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 3 cut(s) 89, 483, 713
MluCI AATT 6 cut(s) 29, 101, 250, 284, 304, 565
Mly113I GGCGCC 1 cut(s) 680
MnlI CCTC 5 cut(s) 271, 337, 580, 687, 750
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 1 cut(s) 142
MspI CCGG 3 cut(s) 14, 82, 548
MspR9I CCNGG 2 cut(s) 82, 548
Mva1269I GAATGC 2 cut(s) 358, 602
MwoI GCNNNNNNNGC 1 cut(s) 487
NarI GGCGCC 1 cut(s) 680
NciI CCSGG 2 cut(s) 82, 548
NdeII GATC 2 cut(s) 68, 159
NlaIII CATG 2 cut(s) 141, 725
NlaIV GGNNCC 3 cut(s) 86, 491, 681
PceI AGGCCT 1 cut(s) 206
PctI GAATGC 2 cut(s) 358, 602
PfeI GAWTC 2 cut(s) 364, 780
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 1 cut(s) 646
PluTI GGCGCC 1 cut(s) 683
PspN4I GGNNCC 3 cut(s) 86, 491, 681
PspPI GGNCC 3 cut(s) 489, 515, 759
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 1 cut(s) 68
RsaI GTAC 2 cut(s) 438, 720
RsaNI GTAC 2 cut(s) 437, 719
RseI CAYNNNNRTG 1 cut(s) 142
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 1 cut(s) 645
Sau3AI GATC 2 cut(s) 68, 159
Sau96I GGNCC 3 cut(s) 489, 515, 759
ScrFI CCNGG 2 cut(s) 82, 548
SduI GDGCHC 3 cut(s) 89, 483, 713
SfaNI GCATC 5 cut(s) 135, 187, 280, 331, 361
SfoI GGCGCC 1 cut(s) 681
SinI GGWCC 1 cut(s) 515
SmiMI CAYNNNNRTG 1 cut(s) 142
SmlI CTYRAG 2 cut(s) 290, 359
SmoI CTYRAG 2 cut(s) 290, 359
Sse9I AATT 6 cut(s) 29, 101, 250, 284, 304, 565
SseBI AGGCCT 1 cut(s) 206
SsiI CCGC 1 cut(s) 600
SspDI GGCGCC 1 cut(s) 679
SspI AATATT 1 cut(s) 187
SspMI CTAG 3 cut(s) 275, 368, 573
StuI AGGCCT 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 80, 546
TasI AATT 6 cut(s) 29, 101, 250, 284, 304, 565
TatI WGTACW 1 cut(s) 718
TfiI GAWTC 2 cut(s) 364, 780
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 718
TseI GCWGC 1 cut(s) 644
TspDTI ATGAA 1 cut(s) 390
TspRI CASTG 1 cut(s) 718
VpaK11BI GGWCC 1 cut(s) 515
XapI RAATTY 3 cut(s) 29, 101, 304
XbaI TCTAGA 2 cut(s) 274, 367
XspI CTAG 3 cut(s) 275, 368, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.