Rw4G007270

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003467
Physical Location & Seq
Reverse (-)
15212636 .. 15215055
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007270.1

Sequence Viewer

Length: 444 bp
ATGTTAATCTCTTTACAGTCAGTTTCATATATATTATTATTATTTTCTTACGTGATTTTCTTTGTCATGCCATTTAAGCATAGAGGAGATATTGCTAGGGCACTGATGTACATGGCGGTATGTTATGGATATCGTCAAACAGATGGAGGCCCAGTTCTTCACCTTTCAGATTCTCCAAAAATTTCAAAGAGTGAGATGGGACTGCTTTCTACGTTGTTAAAGTGGAATGAGATTGATCCCCCTTCAAGAGAGGAGAAATTGAGAAATGACAGAGTATGCAAGTTTTATCAACACAACAGAAATCCTTTCGTTGATCATCCAGAATATGCCACTCTCATATGGAAAGAACATATCTTGAGCCATCATGCTTTCCATCCTCTTCCTAGAAGTCATCAAATTCAAAACAACAAACAAGGAAACTTTAGCAAGCAAGTCATCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

147

Amino Acids

17.27

Weight (kDa)

9.45

Isoelectric Point (pI)

57.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 27 - 108 1.5e-17 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 116
AclWI GGATC 1 cut(s) 230
AcsI RAATTY 2 cut(s) 180, 396
AfaI GTAC 1 cut(s) 110
AgsI TTSAA 3 cut(s) 186, 246, 401
AloI GAACNNNNNNTCC 2 cut(s) 138, 170
AlwI GGATC 1 cut(s) 230
AoxI GGCC 1 cut(s) 148
ApoI RAATTY 2 cut(s) 180, 396
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 103
BccI CCATC 4 cut(s) 137, 190, 369, 381
BclI TGATCA 1 cut(s) 313
BfaI CTAG 2 cut(s) 96, 384
BmgT120I GGNCC 1 cut(s) 149
BmrI ACTGGG 1 cut(s) 146
BmuI ACTGGG 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 376
BsaAI YACGTR 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse1I ACTGG 1 cut(s) 152
BseGI GGATG 2 cut(s) 316, 373
BseNI ACTGG 1 cut(s) 152
BseRI GAGGAG 2 cut(s) 99, 266
BseSI GKGCMC 1 cut(s) 103
BshFI GGCC 1 cut(s) 150
BslFI GGGAC 1 cut(s) 213
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 1 cut(s) 150
Bsp1286I GDGCHC 1 cut(s) 103
Bsp1407I TGTACA 1 cut(s) 108
Bsp143I GATC 2 cut(s) 235, 313
BspACI CCGC 1 cut(s) 116
BspANI GGCC 1 cut(s) 150
BspPI GGATC 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 1 cut(s) 152
BssMI GATC 2 cut(s) 235, 313
Bst4CI ACNGT 1 cut(s) 18
Bst6I CTCTTC 1 cut(s) 384
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 1 cut(s) 428
BstF5I GGATG 2 cut(s) 316, 373
BstKTI GATC 2 cut(s) 238, 316
BstMBI GATC 2 cut(s) 235, 313
BstMWI GCNNNNNNNGC 1 cut(s) 76
BstSLI GKGCMC 1 cut(s) 103
BsuRI GGCC 1 cut(s) 150
BtsCI GGATG 2 cut(s) 316, 373
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 1 cut(s) 428
Cfr13I GGNCC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 109
CviAII CATG 3 cut(s) 67, 112, 365
CviJI RGCY 2 cut(s) 150, 360
CviKI_1 RGCY 2 cut(s) 150, 360
CviQI GTAC 1 cut(s) 109
DpnI GATC 2 cut(s) 237, 315
DpnII GATC 2 cut(s) 235, 313
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco32I GATATC 1 cut(s) 131
EcoRV GATATC 1 cut(s) 131
FaeI CATG 3 cut(s) 70, 115, 368
FaqI GGGAC 1 cut(s) 213
FatI CATG 3 cut(s) 66, 111, 364
FauNDI CATATG 1 cut(s) 338
FbaI TGATCA 1 cut(s) 313
FokI GGATG 2 cut(s) 303, 360
FspBI CTAG 2 cut(s) 96, 384
HaeIII GGCC 1 cut(s) 150
Hin1II CATG 3 cut(s) 70, 115, 368
HinfI GANTC 1 cut(s) 170
HphI GGTGA 1 cut(s) 152
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 3 cut(s) 246, 320, 355
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 1 cut(s) 18
HpyCH4IV ACGT 2 cut(s) 51, 212
HpyCH4V TGCA 1 cut(s) 279
HpyF10VI GCNNNNNNNGC 1 cut(s) 76
HpySE526I ACGT 2 cut(s) 51, 212
Hsp92II CATG 3 cut(s) 70, 115, 368
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 2 cut(s) 235, 313
LpnPI CCDG 3 cut(s) 165, 333, 424
MaeI CTAG 2 cut(s) 96, 384
MaeII ACGT 2 cut(s) 51, 212
MalI GATC 2 cut(s) 237, 315
MboI GATC 2 cut(s) 235, 313
MboII GAAGA 2 cut(s) 149, 371
MhlI GDGCHC 1 cut(s) 103
MluCI AATT 3 cut(s) 180, 257, 396
MnlI CCTC 4 cut(s) 77, 140, 244, 387
MseI TTAA 3 cut(s) 5, 75, 218
MwoI GCNNNNNNNGC 1 cut(s) 76
NdeI CATATG 1 cut(s) 338
NdeII GATC 2 cut(s) 235, 313
NlaIII CATG 3 cut(s) 70, 115, 368
PfeI GAWTC 1 cut(s) 170
Ppu21I YACGTR 1 cut(s) 52
PspPI GGNCC 1 cut(s) 149
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SaqAI TTAA 3 cut(s) 5, 75, 218
Sau3AI GATC 2 cut(s) 235, 313
Sau96I GGNCC 1 cut(s) 149
SduI GDGCHC 1 cut(s) 103
SetI ASST 4 cut(s) 54, 165, 215, 443
SmlI CTYRAG 1 cut(s) 355
SmoI CTYRAG 1 cut(s) 355
Sse9I AATT 3 cut(s) 180, 257, 396
SsiI CCGC 1 cut(s) 116
SspMI CTAG 2 cut(s) 96, 384
TaaI ACNGT 1 cut(s) 18
TaiI ACGT 2 cut(s) 54, 215
TasI AATT 3 cut(s) 180, 257, 396
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 3 cut(s) 5, 75, 218
Tru9I TTAA 3 cut(s) 5, 75, 218
TscAI CASTG 1 cut(s) 108
TspDTI ATGAA 1 cut(s) 15
TspRI CASTG 1 cut(s) 108
XapI RAATTY 2 cut(s) 180, 396
XspI CTAG 2 cut(s) 96, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.