MD16G1242000.v1.1

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003467
Physical Location & Seq
Reverse (-)
26193098 .. 26194418
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1242000.v1.1.491

Sequence Viewer

Length: 345 bp
ATGTACATGGCAGTGTGTTATGGGTTTCATCAACCAGGTGGAGGCCCAGCTCTTCGCCTTTCAGATTCTCCAAAAATTTCAACCGGTGAGATGGGACTGCTTTCTACATTGTTAAAGTGGAACGATAGTGATCCACCTTCAAGAGAAGAGAAGTTAAGAAATGAAAGAGTATGCAAGTTTTATCAACACAATAGAAATCCTTTTGTTGATCATCCAGAATATGCCCGTCTCATATGGAGAAAACATTTTATCCATCGTCTTCCAAGGAGTCATGAAATTCACAACAAAAAGGCAGTTCGGAACAAGGATAAATTCCACAACTATGCGTCCCTAAAAGGCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

115

Amino Acids

13.41

Weight (kDa)

9.85

Isoelectric Point (pI)

64.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 1 - 73 1.9e-11 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 125
AcsI RAATTY 3 cut(s) 75, 276, 311
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 41
AgeI ACCGGT 1 cut(s) 83
AgsI TTSAA 2 cut(s) 81, 141
AjnI CCWGG 1 cut(s) 34
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
Alw26I GTCTC 1 cut(s) 233
AlwI GGATC 1 cut(s) 125
AoxI GGCC 1 cut(s) 43
ApoI RAATTY 3 cut(s) 75, 276, 311
AsiGI ACCGGT 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 251
BccI CCATC 2 cut(s) 85, 261
BciT130I CCWGG 1 cut(s) 36
BclI TGATCA 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 233
Bme1390I CCNGG 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 44
BmrFI CCNGG 1 cut(s) 36
BpiI GAAGAC 1 cut(s) 251
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 263
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse118I RCCGGY 1 cut(s) 83
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 263
BseGI GGATG 1 cut(s) 211
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 41
BseYI CCCAGC 1 cut(s) 46
BshFI GGCC 1 cut(s) 45
BshTI ACCGGT 1 cut(s) 83
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 2 cut(s) 108, 313
BslI CCNNNNNNNGG 1 cut(s) 41
BsmAI GTCTC 1 cut(s) 233
BsmBI CGTCTC 1 cut(s) 233
BsmFI GGGAC 2 cut(s) 108, 313
BsnI GGCC 1 cut(s) 45
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 130, 208
BspANI GGCC 1 cut(s) 45
BspHI TCATGA 1 cut(s) 271
BspPI GGATC 1 cut(s) 125
BspQI GCTCTTC 1 cut(s) 57
BsrFI RCCGGY 1 cut(s) 83
BsrGI TGTACA 1 cut(s) 3
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 263
BssMI GATC 2 cut(s) 130, 208
BssT1I CCWWGG 1 cut(s) 263
Bst2UI CCWGG 1 cut(s) 36
Bst6I CTCTTC 2 cut(s) 57, 141
BstAUI TGTACA 1 cut(s) 3
BstF5I GGATG 1 cut(s) 211
BstKTI GATC 2 cut(s) 133, 211
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 2 cut(s) 130, 208
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstV2I GAAGAC 1 cut(s) 251
BsuRI GGCC 1 cut(s) 45
BtsCI GGATG 1 cut(s) 211
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
CciI TCATGA 1 cut(s) 271
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 44
CseI GACGC 1 cut(s) 315
CsiI ACCWGGT 1 cut(s) 34
Csp6I GTAC 1 cut(s) 4
CspAI ACCGGT 1 cut(s) 83
CviAII CATG 2 cut(s) 7, 272
CviJI RGCY 2 cut(s) 45, 50
CviKI_1 RGCY 2 cut(s) 45, 50
CviQI GTAC 1 cut(s) 4
DpnI GATC 2 cut(s) 132, 210
DpnII GATC 2 cut(s) 130, 208
Eam1104I CTCTTC 2 cut(s) 57, 141
EarI CTCTTC 2 cut(s) 57, 141
Eco130I CCWWGG 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 263
Esp3I CGTCTC 1 cut(s) 233
FaeI CATG 2 cut(s) 10, 275
FaiI YATR 8 cut(s) 8, 21, 172, 222, 233, 235, 273, 324
FaqI GGGAC 2 cut(s) 108, 313
FatI CATG 2 cut(s) 6, 271
FauNDI CATATG 1 cut(s) 233
FbaI TGATCA 1 cut(s) 208
FokI GGATG 1 cut(s) 198
GsaI CCCAGC 1 cut(s) 50
HaeIII GGCC 1 cut(s) 45
HapII CCGG 1 cut(s) 84
HgaI GACGC 1 cut(s) 315
Hin1II CATG 2 cut(s) 10, 275
HinfI GANTC 2 cut(s) 65, 268
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 98
Hpy188I TCNGA 2 cut(s) 64, 300
Hpy188III TCNNGA 3 cut(s) 141, 215, 272
HpyAV CCTTC 1 cut(s) 147
HpyCH4V TGCA 1 cut(s) 174
Hsp92II CATG 2 cut(s) 10, 275
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 2 cut(s) 130, 208
LguI GCTCTTC 1 cut(s) 57
LpnPI CCDG 5 cut(s) 21, 48, 60, 97, 228
MabI ACCWGGT 1 cut(s) 34
MalI GATC 2 cut(s) 132, 210
MboI GATC 2 cut(s) 130, 208
MboII GAAGA 3 cut(s) 44, 158, 251
MluCI AATT 3 cut(s) 75, 276, 311
MlyI GAGTC 1 cut(s) 277
MnlI CCTC 1 cut(s) 35
MseI TTAA 3 cut(s) 113, 155, 343
MslI CAYNNNNRTG 2 cut(s) 11, 321
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 233
NdeII GATC 2 cut(s) 130, 208
NlaIII CATG 2 cut(s) 10, 275
PagI TCATGA 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 57
PfeI GAWTC 1 cut(s) 65
PinAI ACCGGT 1 cut(s) 83
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 34
PspFI CCCAGC 1 cut(s) 46
PspGI CCWGG 1 cut(s) 34
PspPI GGNCC 1 cut(s) 44
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 11, 321
SapI GCTCTTC 1 cut(s) 57
SaqAI TTAA 3 cut(s) 113, 155, 343
Sau3AI GATC 2 cut(s) 130, 208
Sau96I GGNCC 1 cut(s) 44
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 36
SetI ASST 3 cut(s) 40, 52, 139
SexAI ACCWGGT 1 cut(s) 34
SmiMI CAYNNNNRTG 2 cut(s) 11, 321
Sse9I AATT 3 cut(s) 75, 276, 311
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 1 cut(s) 263
TasI AATT 3 cut(s) 75, 276, 311
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 3 cut(s) 113, 155, 343
Tru9I TTAA 3 cut(s) 113, 155, 343
TscAI CASTG 1 cut(s) 18
TspDTI ATGAA 3 cut(s) 17, 177, 288
TspRI CASTG 1 cut(s) 18
XapI RAATTY 3 cut(s) 75, 276, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.