Rroxscaffold_5G00343520

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003467
Physical Location & Seq
Reverse (-)
13029020 .. 13035442
6423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343520.1

Sequence Viewer

Length: 1134 bp
ATGGCCACCAAATTCCATTGCACAAAACCCGTCGTAGTCTTTAAGCAGAGACCCACCTTCTTCCGGAGGCCCCAGAACCTCAAATTCAAGCCTTTTAATCGAAACCCAGATCATATTTTGGTCATTCCAATCACCTCCACTATTTCTACAAGGGGCATATGGGATTTCACAAAGTGCATCAATATCTCATGGAGATGGGTTTCTCAGATTATCTCTGTTTACTTGTTTTTTCTCAATATTGTTGCTGATGCTCGGGCCTATGATTACCCATCTCCTTCTTCTTCTTCTTCTTCTTCTTCTGTTTATGCTTGTGAAGATGTTAACAATTACTATGCCAATGTGGAGCATTTAGAGGGCAAACCACTCAAGAAGAAACTGAATTCCATCATTGGCAAGCATCAATCTTTATCTTACAGAGAGGTGTGGAATGCTCTCAAGATTCTAGATGCTTCGGATGTTGACAACCCAGAAGCTTCATCAGGGATAGTTGAAATTTACTCCGTGAAGGTTGTACCAAAGTCATTAGCTGGAAAGCCTGAAGGATGGAATAGAGAGCACTTATGGCCTCGTTCTTATGGTTTGAAAAATGGTCCATCCCTGACTGATATACACAACATTCGCCCTGCAGATGTGAATGTCAATTCCTCCAGGGGGAACAAATACTACGGAGAATGCAGTGTTAATTCGGCTAAATGTTTGAAGCCAGCAAATAAAGAAGCTGCTTTGGACACAGAAACTGACAAGGATAGATGGGCACCACCTAAACAGCATAGAGGAGATATTGCTAGGGCACTGATGTACATGGCGGTATGTTATGGATATCGTCAACCAGATGGAGGCCCAGTTCTTCACCTTTCAGATTCTCCAAAAATTTCAAAGAGTGAGATGGGACTGCTTTCTACGTTGTTAAAGTGGAATGAGATTGATCCCCCTTCAAGAGAGGAGAAATTGAGAAATGACAGAGTATGCAAGTTTTATCAACACAACAGAAATCCTTTCGTTGACCATCCAGAATATGCCACTCTCATATGGAAAGAACATATCTTGAGCCATCATGCTTTCCATCCTCTTCCTAGAAGTCATCAAATTAAAAACAACAAACAGGGAAACTTTAGCAAGCAAGTCATCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

377

Amino Acids

43.17

Weight (kDa)

9.47

Isoelectric Point (pI)

54.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 120 - 338 2.1e-39 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 754
AccIII TCCGGA 1 cut(s) 63
AciI CCGC 1 cut(s) 806
AclWI GGATC 1 cut(s) 920
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 5 cut(s) 11, 83, 379, 492, 870
AcuI CTGAAG 1 cut(s) 558
AdeI CACNNNGTG 1 cut(s) 174
AfaI GTAC 2 cut(s) 513, 800
AfiI CCNNNNNNNGG 3 cut(s) 63, 651, 836
AgsI TTSAA 6 cut(s) 88, 491, 583, 700, 876, 936
AjnI CCWGG 1 cut(s) 647
AloI GAACNNNNNNTCC 2 cut(s) 828, 860
AluBI AGCT 3 cut(s) 473, 527, 719
AluI AGCT 3 cut(s) 473, 527, 719
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 1 cut(s) 43
AlwI GGATC 1 cut(s) 920
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 252
Aor13HI TCCGGA 1 cut(s) 63
AoxI GGCC 5 cut(s) 3, 68, 255, 563, 838
ApeKI GCWGC 1 cut(s) 719
ApoI RAATTY 5 cut(s) 11, 83, 379, 492, 870
AspS9I GGNCC 4 cut(s) 69, 255, 590, 839
AsuHPI GGTGA 2 cut(s) 124, 842
AvaI CYCGRG 1 cut(s) 252
AvaII GGWCC 1 cut(s) 590
BaeGI GKGCMC 2 cut(s) 757, 793
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 754
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 1 cut(s) 706
BciT130I CCWGG 1 cut(s) 649
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 3 cut(s) 443, 786, 1074
BfmI CTRYAG 1 cut(s) 624
BisI GCNGC 1 cut(s) 720
BlsI GCNGC 1 cut(s) 721
Bme1390I CCNGG 1 cut(s) 649
Bme18I GGWCC 1 cut(s) 590
BmeT110I CYCGRG 1 cut(s) 252
BmgT120I GGNCC 4 cut(s) 69, 255, 590, 839
BmiI GGNNCC 2 cut(s) 71, 756
BmrFI CCNGG 1 cut(s) 649
BmrI ACTGGG 1 cut(s) 836
BmsI GCATC 4 cut(s) 186, 238, 406, 436
BmuI ACTGGG 1 cut(s) 836
BpmI CTGGAG 1 cut(s) 631
BpuEI CTTGAG 3 cut(s) 350, 419, 1066
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 1 cut(s) 648
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 651, 836
Bse1I ACTGG 1 cut(s) 842
Bse3DI GCAATG 1 cut(s) 16
BseAI TCCGGA 1 cut(s) 63
BseBI CCWGG 1 cut(s) 649
BseDI CCNNGG 1 cut(s) 648
BseGI GGATG 5 cut(s) 460, 548, 593, 1006, 1063
BseLI CCNNNNNNNGG 3 cut(s) 63, 651, 836
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 218
BseNI ACTGG 1 cut(s) 842
BseRI GAGGAG 2 cut(s) 789, 956
BseSI GKGCMC 2 cut(s) 757, 793
BseXI GCAGC 1 cut(s) 706
BshFI GGCC 5 cut(s) 5, 70, 257, 565, 840
BshNI GGYRCC 1 cut(s) 754
BsiHKAI GWGCWC 1 cut(s) 558
BsiHKCI CYCGRG 1 cut(s) 252
BsiSI CCGG 1 cut(s) 64
BslFI GGGAC 1 cut(s) 903
BslI CCNNNNNNNGG 3 cut(s) 63, 651, 836
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 903
BsmI GAATGC 2 cut(s) 433, 677
BsnI GGCC 5 cut(s) 5, 70, 257, 565, 840
Bso31I GGTCTC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 252
Bsp1286I GDGCHC 3 cut(s) 558, 757, 793
Bsp13I TCCGGA 1 cut(s) 63
Bsp1407I TGTACA 1 cut(s) 798
Bsp143I GATC 2 cut(s) 109, 925
BspACI CCGC 1 cut(s) 806
BspANI GGCC 5 cut(s) 5, 70, 257, 565, 840
BspCNI CTCAG 1 cut(s) 217
BspEI TCCGGA 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 71, 756
BspMAI CTGCAG 1 cut(s) 628
BspPI GGATC 1 cut(s) 920
BspT107I GGYRCC 1 cut(s) 754
BspTNI GGTCTC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 798
BsrI ACTGG 1 cut(s) 842
BssECI CCNNGG 1 cut(s) 648
BssMI GATC 2 cut(s) 109, 925
Bst2UI CCWGG 1 cut(s) 649
Bst6I CTCTTC 1 cut(s) 1074
BstAUI TGTACA 1 cut(s) 798
BstC8I GCNNGC 3 cut(s) 395, 705, 1118
BstDEI CTNAG 1 cut(s) 204
BstF5I GGATG 5 cut(s) 460, 548, 593, 1006, 1063
BstKTI GATC 2 cut(s) 112, 928
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 2 cut(s) 109, 925
BstMWI GCNNNNNNNGC 1 cut(s) 562
BstNI CCWGG 1 cut(s) 649
BstSCI CCNGG 1 cut(s) 647
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 2 cut(s) 757, 793
BstV1I GCAGC 1 cut(s) 706
BsuRI GGCC 5 cut(s) 5, 70, 257, 565, 840
BtsCI GGATG 5 cut(s) 460, 548, 593, 1006, 1063
BtsI GCAGTG 1 cut(s) 682
BtsIMutI CAGTG 2 cut(s) 682, 791
Cac8I GCNNGC 3 cut(s) 395, 705, 1118
CaiI CAGNNNCTG 1 cut(s) 737
Cfr13I GGNCC 4 cut(s) 69, 255, 590, 839
Csp6I GTAC 2 cut(s) 512, 799
CviAII CATG 3 cut(s) 189, 802, 1055
CviQI GTAC 2 cut(s) 512, 799
DdeI CTNAG 1 cut(s) 204
DpnI GATC 2 cut(s) 111, 927
DpnII GATC 2 cut(s) 109, 925
DraIII CACNNNGTG 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1074
EarI CTCTTC 1 cut(s) 1074
Eco31I GGTCTC 1 cut(s) 43
Eco32I GATATC 1 cut(s) 821
Eco47I GGWCC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 69
EcoRI GAATTC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 647
EcoRV GATATC 1 cut(s) 821
FaeI CATG 3 cut(s) 192, 805, 1058
FaqI GGGAC 1 cut(s) 903
FatI CATG 3 cut(s) 188, 801, 1054
FauNDI CATATG 2 cut(s) 158, 1028
Fnu4HI GCNGC 1 cut(s) 720
FokI GGATG 5 cut(s) 467, 555, 580, 993, 1050
Fsp4HI GCNGC 1 cut(s) 720
FspBI CTAG 3 cut(s) 443, 786, 1074
GluI GCNGC 1 cut(s) 720
GsuI CTGGAG 1 cut(s) 631
HaeIII GGCC 5 cut(s) 5, 70, 257, 565, 840
HapII CCGG 1 cut(s) 64
Hin1II CATG 3 cut(s) 192, 805, 1058
HincII GTYRAC 4 cut(s) 322, 460, 827, 1003
HindII GTYRAC 4 cut(s) 322, 460, 827, 1003
HindIII AAGCTT 1 cut(s) 471
HinfI GANTC 2 cut(s) 439, 860
HpaI GTTAAC 1 cut(s) 322
HpaII CCGG 1 cut(s) 64
HphI GGTGA 2 cut(s) 124, 842
Hpy166II GTNNAC 5 cut(s) 220, 322, 460, 827, 1003
Hpy188I TCNGA 3 cut(s) 207, 454, 859
Hpy188III TCNNGA 7 cut(s) 64, 367, 436, 443, 936, 1010, 1045
Hpy8I GTNNAC 5 cut(s) 220, 322, 460, 827, 1003
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 5 cut(s) 67, 285, 499, 533, 942
HpyCH4IV ACGT 1 cut(s) 902
HpyCH4V TGCA 5 cut(s) 21, 177, 626, 675, 969
HpyF10VI GCNNNNNNNGC 1 cut(s) 562
HpyF3I CTNAG 1 cut(s) 204
HpySE526I ACGT 1 cut(s) 902
Hsp92II CATG 3 cut(s) 192, 805, 1058
Kpn2I TCCGGA 1 cut(s) 63
KspAI GTTAAC 1 cut(s) 322
Kzo9I GATC 2 cut(s) 109, 925
LmnI GCTCC 1 cut(s) 343
Lsp1109I GCAGC 1 cut(s) 706
LweI GCATC 4 cut(s) 186, 238, 406, 436
MaeI CTAG 3 cut(s) 443, 786, 1074
MaeII ACGT 1 cut(s) 902
MalI GATC 2 cut(s) 111, 927
MboI GATC 2 cut(s) 109, 925
MhlI GDGCHC 3 cut(s) 558, 757, 793
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 63
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 42, 96, 321, 681, 908, 1089
MslI CAYNNNNRTG 1 cut(s) 193
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 64
MspR9I CCNGG 1 cut(s) 649
Mva1269I GAATGC 2 cut(s) 433, 677
MvaI CCWGG 1 cut(s) 649
MwoI GCNNNNNNNGC 1 cut(s) 562
NdeI CATATG 2 cut(s) 158, 1028
NdeII GATC 2 cut(s) 109, 925
NlaIII CATG 3 cut(s) 192, 805, 1058
NlaIV GGNNCC 2 cut(s) 71, 756
PctI GAATGC 2 cut(s) 433, 677
PfeI GAWTC 2 cut(s) 439, 860
PkrI GCNGC 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 647
PspGI CCWGG 1 cut(s) 647
PspN4I GGNNCC 2 cut(s) 71, 756
PspPI GGNCC 4 cut(s) 69, 255, 590, 839
PsrI GAACNNNNNNTAC 2 cut(s) 647, 679
PstI CTGCAG 1 cut(s) 628
PstNI CAGNNNCTG 1 cut(s) 737
RsaI GTAC 2 cut(s) 513, 800
RsaNI GTAC 2 cut(s) 512, 799
RseI CAYNNNNRTG 1 cut(s) 193
SaqAI TTAA 6 cut(s) 42, 96, 321, 681, 908, 1089
SatI GCNGC 1 cut(s) 720
Sau3AI GATC 2 cut(s) 109, 925
Sau96I GGNCC 4 cut(s) 69, 255, 590, 839
ScrFI CCNGG 1 cut(s) 649
SduI GDGCHC 3 cut(s) 558, 757, 793
SfaNI GCATC 4 cut(s) 186, 238, 406, 436
SfcI CTRYAG 1 cut(s) 624
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 193
SmlI CTYRAG 3 cut(s) 365, 434, 1045
SmoI CTYRAG 3 cut(s) 365, 434, 1045
SsiI CCGC 1 cut(s) 806
SspI AATATT 1 cut(s) 238
SspMI CTAG 3 cut(s) 443, 786, 1074
StyD4I CCNGG 1 cut(s) 647
TaiI ACGT 1 cut(s) 905
TaqI TCGA 1 cut(s) 100
TatI WGTACW 1 cut(s) 798
TfiI GAWTC 2 cut(s) 439, 860
Tru1I TTAA 6 cut(s) 42, 96, 321, 681, 908, 1089
Tru9I TTAA 6 cut(s) 42, 96, 321, 681, 908, 1089
TscAI CASTG 2 cut(s) 682, 798
TseI GCWGC 1 cut(s) 719
TspDTI ATGAA 1 cut(s) 465
TspGWI ACGGA 2 cut(s) 490, 681
TspRI CASTG 2 cut(s) 682, 798
VpaK11BI GGWCC 1 cut(s) 590
XapI RAATTY 5 cut(s) 11, 83, 379, 492, 870
XbaI TCTAGA 1 cut(s) 442
XspI CTAG 3 cut(s) 443, 786, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.