RchiOBHm_Chr4g0398551

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003467
Physical Location & Seq
Reverse (-)
15105412 .. 15111763
6352 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37072

Sequence Viewer

Length: 1143 bp
ATGCAGTCTTCTCCAGTAATGGCCACCAAATTCCATTGCGCAAAACCCGTCGTTGTCTGTAAGCAGAGACCCAACTTTTTCCGGGGCCCCGAGAACTTCAAACTCAAGCCTTTTAATCGAAACCCAGATCATATTTTGGTCAGTCCAATCACCTCCACCATTTCTACAAGGGGCATATGGGATTTCACAAAGTGCATCAATATCTCATGGAGATGGGTTTCTCAGATTCTCTCTGTTTACTTGCTTTTTCTCAATATTGTTGCTGATGCTCAGGCCTATGATTACCCATCTCCTTCTTCTTCTTCTTCTGTTTATGCTTGTGAAGATGTTAACAATTACTATGCCAATGTGGAGCATTTAGAGGGTAAATCACTCAAGAAGAAACTGAATTCCATCATTGGCAAGCATCAATCTTTATCTTACAGAGAGGTGTGGAATGCTCTCAAGATTCTAGATGCTTCGGATGTTGACAACCCAGAAGCTTCATCAGGGATAGTTGAAATTTACTCCGTGAAGGTTGTACCAAAGTCATTAGCTGGAAAGCCTGAAGGATGGAATAGAGAGCACTTATGGCCTCGTTCTTATGGTTTGAAAAATGGTCCATCCCTGACTGATATACACAACATTCGCCCTGCAGATGTGAATGTCAATTCCTCCAGGGGGAACAAATACTACGGAGAATGCAGTGTTAATTCGGCTAAATGTTTGAAGCCAGCAAGTAAAGAAGCTGCTTTGGACACAGAAACTGACAAGGATAGATGGGCACCACCTAAACAGCATAGAGGAGATATTGCTAGGGCACTGATGTACATGGCGGTATGTTATGGATATCGTCAAACAGATGGAGGCCCAGTTCTTCACCTTTCAGATTCTCCAAAAATTTCAAAGAGTGAGATGGGACTGCTTTCTACGTTGTTAAAGTGGAATGAGATTGATCCCCCTTCAAGAGAGGAGAAATTGAGAAATGACAGAGTATGCAAGTTTTATCAACACAACAGAAATCCTTTCGTTGATCATCCAGAATATGCCACTCTCATATGGAAAGAACATATCTTGAGCCATCATGCTTTCCATCCTCTTCCTAGAAGTCATCAAATTCAAAACAACAAACAAGGAAACTTTAGCAAGCAAGTCATCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

380

Amino Acids

43.26

Weight (kDa)

9.25

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 122 - 341 1.3e-39 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 763
AciI CCGC 1 cut(s) 815
AclWI GGATC 1 cut(s) 929
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 5 cut(s) 29, 388, 501, 879, 1095
AcuI CTGAAG 1 cut(s) 567
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 2 cut(s) 522, 809
AfiI CCNNNNNNNGG 1 cut(s) 660
AgsI TTSAA 7 cut(s) 100, 500, 592, 709, 885, 945, 1100
AjnI CCWGG 1 cut(s) 656
AloI GAACNNNNNNTCC 2 cut(s) 837, 869
AluBI AGCT 3 cut(s) 482, 536, 728
AluI AGCT 3 cut(s) 482, 536, 728
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 1 cut(s) 929
AlwNI CAGNNNCTG 1 cut(s) 746
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 5 cut(s) 21, 85, 273, 572, 847
ApaI GGGCCC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 728
ApoI RAATTY 5 cut(s) 29, 388, 501, 879, 1095
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 4 cut(s) 85, 86, 599, 848
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 2 cut(s) 142, 851
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 1 cut(s) 599
BaeGI GKGCMC 3 cut(s) 89, 766, 802
BalI TGGCCA 1 cut(s) 23
BanI GGYRCC 1 cut(s) 763
BanII GRGCYC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 567
BbvI GCAGC 1 cut(s) 715
BciT130I CCWGG 1 cut(s) 658
BclI TGATCA 1 cut(s) 1012
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 3 cut(s) 452, 795, 1083
BfmI CTRYAG 1 cut(s) 633
BisI GCNGC 1 cut(s) 729
BlsI GCNGC 1 cut(s) 730
Bme1390I CCNGG 2 cut(s) 83, 658
Bme18I GGWCC 1 cut(s) 599
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 4 cut(s) 85, 86, 599, 848
BmiI GGNNCC 4 cut(s) 86, 87, 88, 765
BmrFI CCNGG 2 cut(s) 83, 658
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 4 cut(s) 204, 256, 415, 445
BmuI ACTGGG 1 cut(s) 845
BpmI CTGGAG 1 cut(s) 640
Bpu10I CCTNAGC 1 cut(s) 270
BpuEI CTTGAG 4 cut(s) 89, 359, 428, 1075
BpuMI CCSGG 1 cut(s) 83
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 2 cut(s) 82, 657
BsaXI ACNNNNNCTCC 2 cut(s) 837, 867
Bsc4I CCNNNNNNNGG 1 cut(s) 660
Bse1I ACTGG 2 cut(s) 14, 851
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 658
BseDI CCNNGG 2 cut(s) 82, 657
BseGI GGATG 5 cut(s) 469, 557, 602, 1015, 1072
BseLI CCNNNNNNNGG 1 cut(s) 660
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 2 cut(s) 236, 284
BseNI ACTGG 2 cut(s) 14, 851
BseRI GAGGAG 2 cut(s) 798, 965
BseSI GKGCMC 3 cut(s) 89, 766, 802
BseXI GCAGC 1 cut(s) 715
BshFI GGCC 5 cut(s) 23, 87, 275, 574, 849
BshNI GGYRCC 1 cut(s) 763
BsiHKAI GWGCWC 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 912
BslI CCNNNNNNNGG 1 cut(s) 660
BsmAI GTCTC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 912
BsmI GAATGC 2 cut(s) 442, 686
BsnI GGCC 5 cut(s) 23, 87, 275, 574, 849
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 1 cut(s) 89
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 4 cut(s) 89, 567, 766, 802
Bsp1407I TGTACA 1 cut(s) 807
Bsp143I GATC 3 cut(s) 127, 934, 1012
BspACI CCGC 1 cut(s) 815
BspANI GGCC 5 cut(s) 23, 87, 275, 574, 849
BspCNI CTCAG 2 cut(s) 235, 283
BspLI GGNNCC 4 cut(s) 86, 87, 88, 765
BspMAI CTGCAG 1 cut(s) 637
BspPI GGATC 1 cut(s) 929
BspT107I GGYRCC 1 cut(s) 763
BspTNI GGTCTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 807
BsrI ACTGG 2 cut(s) 14, 851
BssECI CCNNGG 2 cut(s) 82, 657
BssMI GATC 3 cut(s) 127, 934, 1012
Bst2UI CCWGG 1 cut(s) 658
Bst6I CTCTTC 1 cut(s) 1083
BstAUI TGTACA 1 cut(s) 807
BstC8I GCNNGC 3 cut(s) 404, 714, 1127
BstDEI CTNAG 2 cut(s) 222, 270
BstF5I GGATG 5 cut(s) 469, 557, 602, 1015, 1072
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 3 cut(s) 130, 937, 1015
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 3 cut(s) 127, 934, 1012
BstMWI GCNNNNNNNGC 1 cut(s) 571
BstNI CCWGG 1 cut(s) 658
BstSCI CCNGG 2 cut(s) 81, 656
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 3 cut(s) 89, 766, 802
BstV1I GCAGC 1 cut(s) 715
BsuRI GGCC 5 cut(s) 23, 87, 275, 574, 849
BtsCI GGATG 5 cut(s) 469, 557, 602, 1015, 1072
BtsI GCAGTG 1 cut(s) 691
BtsIMutI CAGTG 2 cut(s) 691, 800
Cac8I GCNNGC 3 cut(s) 404, 714, 1127
CaiI CAGNNNCTG 1 cut(s) 746
CfoI GCGC 1 cut(s) 41
Cfr13I GGNCC 4 cut(s) 85, 86, 599, 848
Csp6I GTAC 2 cut(s) 521, 808
CviAII CATG 3 cut(s) 207, 811, 1064
CviQI GTAC 2 cut(s) 521, 808
DdeI CTNAG 2 cut(s) 222, 270
DpnI GATC 3 cut(s) 129, 936, 1014
DpnII GATC 3 cut(s) 127, 934, 1012
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 1083
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 830
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 89
EcoO109I RGGNCCY 2 cut(s) 85, 86
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 656
EcoRV GATATC 1 cut(s) 830
EcoT38I GRGCYC 1 cut(s) 89
FaeI CATG 3 cut(s) 210, 814, 1067
FaqI GGGAC 1 cut(s) 912
FatI CATG 3 cut(s) 206, 810, 1063
FauNDI CATATG 2 cut(s) 176, 1037
FbaI TGATCA 1 cut(s) 1012
Fnu4HI GCNGC 1 cut(s) 729
FokI GGATG 5 cut(s) 476, 564, 589, 1002, 1059
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 729
FspBI CTAG 3 cut(s) 452, 795, 1083
FspI TGCGCA 1 cut(s) 40
GlaI GCGC 1 cut(s) 40
GluI GCNGC 1 cut(s) 729
GsuI CTGGAG 1 cut(s) 640
HaeIII GGCC 5 cut(s) 23, 87, 275, 574, 849
HapII CCGG 1 cut(s) 82
HhaI GCGC 1 cut(s) 41
Hin1II CATG 3 cut(s) 210, 814, 1067
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HincII GTYRAC 2 cut(s) 331, 469
HindII GTYRAC 2 cut(s) 331, 469
HindIII AAGCTT 1 cut(s) 480
HinfI GANTC 3 cut(s) 226, 448, 869
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 1 cut(s) 82
HphI GGTGA 2 cut(s) 142, 851
Hpy166II GTNNAC 3 cut(s) 238, 331, 469
Hpy188I TCNGA 4 cut(s) 225, 463, 868, 1139
Hpy188III TCNNGA 6 cut(s) 376, 445, 452, 945, 1019, 1054
Hpy8I GTNNAC 3 cut(s) 238, 331, 469
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 4 cut(s) 303, 508, 542, 951
HpyCH4IV ACGT 1 cut(s) 911
HpyCH4V TGCA 5 cut(s) 4, 195, 635, 684, 978
HpyF10VI GCNNNNNNNGC 1 cut(s) 571
HpyF3I CTNAG 2 cut(s) 222, 270
HpySE526I ACGT 1 cut(s) 911
Hsp92II CATG 3 cut(s) 210, 814, 1067
HspAI GCGC 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 1012
KspAI GTTAAC 1 cut(s) 331
Kzo9I GATC 3 cut(s) 127, 934, 1012
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 1 cut(s) 715
LweI GCATC 4 cut(s) 204, 256, 415, 445
MaeI CTAG 3 cut(s) 452, 795, 1083
MaeII ACGT 1 cut(s) 911
MalI GATC 3 cut(s) 129, 936, 1014
MboI GATC 3 cut(s) 127, 934, 1012
MboII GAAGA 8 cut(s) 288, 291, 294, 297, 335, 391, 848, 1070
MhlI GDGCHC 4 cut(s) 89, 567, 766, 802
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 9 cut(s) 29, 334, 388, 501, 649, 691, 879, 956, 1095
MluNI TGGCCA 1 cut(s) 23
MnlI CCTC 9 cut(s) 163, 355, 421, 585, 664, 776, 839, 943, 1086
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
MseI TTAA 4 cut(s) 114, 330, 690, 917
MslI CAYNNNNRTG 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 2 cut(s) 83, 658
Mva1269I GAATGC 2 cut(s) 442, 686
MvaI CCWGG 1 cut(s) 658
MwoI GCNNNNNNNGC 1 cut(s) 571
NciI CCSGG 1 cut(s) 83
NdeI CATATG 2 cut(s) 176, 1037
NdeII GATC 3 cut(s) 127, 934, 1012
NlaIII CATG 3 cut(s) 210, 814, 1067
NlaIV GGNNCC 4 cut(s) 86, 87, 88, 765
NsbI TGCGCA 1 cut(s) 40
PceI AGGCCT 1 cut(s) 275
PctI GAATGC 2 cut(s) 442, 686
PfeI GAWTC 3 cut(s) 226, 448, 869
PkrI GCNGC 1 cut(s) 730
Psp6I CCWGG 1 cut(s) 656
PspGI CCWGG 1 cut(s) 656
PspN4I GGNNCC 4 cut(s) 86, 87, 88, 765
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 4 cut(s) 85, 86, 599, 848
PsrI GAACNNNNNNTAC 2 cut(s) 656, 688
PstI CTGCAG 1 cut(s) 637
PstNI CAGNNNCTG 1 cut(s) 746
RsaI GTAC 2 cut(s) 522, 809
RsaNI GTAC 2 cut(s) 521, 808
RseI CAYNNNNRTG 1 cut(s) 211
SaqAI TTAA 4 cut(s) 114, 330, 690, 917
SatI GCNGC 1 cut(s) 729
Sau3AI GATC 3 cut(s) 127, 934, 1012
Sau96I GGNCC 4 cut(s) 85, 86, 599, 848
ScrFI CCNGG 2 cut(s) 83, 658
SduI GDGCHC 4 cut(s) 89, 567, 766, 802
SetI ASST 9 cut(s) 155, 432, 484, 519, 538, 730, 772, 864, 914
SfaNI GCATC 4 cut(s) 204, 256, 415, 445
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 211
SmlI CTYRAG 4 cut(s) 104, 374, 443, 1054
SmoI CTYRAG 4 cut(s) 104, 374, 443, 1054
Sse9I AATT 9 cut(s) 29, 334, 388, 501, 649, 691, 879, 956, 1095
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 1 cut(s) 815
SspI AATATT 1 cut(s) 256
SspMI CTAG 3 cut(s) 452, 795, 1083
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 2 cut(s) 81, 656
TaiI ACGT 1 cut(s) 914
TaqI TCGA 1 cut(s) 118
TasI AATT 9 cut(s) 29, 334, 388, 501, 649, 691, 879, 956, 1095
TatI WGTACW 1 cut(s) 807
TfiI GAWTC 3 cut(s) 226, 448, 869
Tru1I TTAA 4 cut(s) 114, 330, 690, 917
Tru9I TTAA 4 cut(s) 114, 330, 690, 917
TscAI CASTG 2 cut(s) 691, 807
TseI GCWGC 1 cut(s) 728
TspDTI ATGAA 1 cut(s) 474
TspGWI ACGGA 2 cut(s) 499, 690
TspRI CASTG 2 cut(s) 691, 807
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 5 cut(s) 29, 388, 501, 879, 1095
XbaI TCTAGA 1 cut(s) 451
XspI CTAG 3 cut(s) 452, 795, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.