Rh4BG083800

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003467
Physical Location & Seq
Reverse (-)
14522275 .. 14528894
6620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG083800.1

Sequence Viewer

Length: 807 bp
ATGCAGTCTTCTCCAGTAATGGCCACCAAATTCCATTGCACAAAACCCGTCGTTGTCTGTAAGCAGAGACCCAACTTTTTCCGGGGCCCCGAGAACTTCAAACTCAAGCCTTTTAATCGAAACCCAGATCATATTTTGGTCAGTCCAATCACCTCCACCATTTCTACAAGGGGCATATGGGATTTCACAAAGTGCATCAATATCTCATGGAGATGGGTTTCTCGGATTCTCTCTGTTTACTTGCTTTTTCTCAATATTGTTGCTGATGCTCAGGCCTATGATTACCCATCTCCATCTTCTTCTTCTTCTTCTGTTTATGCTTGTGAAGATGTTAACAACTACTATGCCAATGTGGAGCATTTAGAGGGTAAATCACTCAAGAAGAAACTGAATTCCATCATTGGCAAGCATCAATCTTTATCTTACAGAGAGGTGTGGAATGCTCTCAAGATTCTAGATGCTTCGGATGTTGACAACCCAGAAGCTTCATCAGGGATAGTTGAAATTTACTCCGTGAAGGTTGTACCAAAGTCATTAGCTGGAAAGCCTGAAGGATGGAATAGAGAGCACTTATGGCCTCGTTCTTATGGTTTGAAAAATGGTCCATCCCTGACTGATATACACAACATTCGCCCTGCAGATGTGAATGTCAATTCCTCCAGGGGGAACAAATACTACGGAGAATGCAGTGTTAATTCGGCTAAATGTTTGAAGCCAGCAAATAAAGAAGCTGCTTTGGACACAGAAACTGACAAGGATAGATGGGCACCACCTAAACAGGTTTTTCACTGTGCATATCTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

268

Amino Acids

30.21

Weight (kDa)

9.12

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 123 - 241 4.8e-17 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 766
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 3 cut(s) 29, 391, 504
AcuI CTGAAG 1 cut(s) 570
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 1 cut(s) 525
AfiI CCNNNNNNNGG 1 cut(s) 663
AgsI TTSAA 4 cut(s) 100, 503, 595, 712
AjnI CCWGG 1 cut(s) 659
AluBI AGCT 3 cut(s) 485, 539, 731
AluI AGCT 3 cut(s) 485, 539, 731
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 1 cut(s) 61
AlwNI CAGNNNCTG 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 4 cut(s) 21, 85, 273, 575
ApaI GGGCCC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 731
ApoI RAATTY 3 cut(s) 29, 391, 504
AspS9I GGNCC 3 cut(s) 85, 86, 602
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 2 cut(s) 89, 769
BalI TGGCCA 1 cut(s) 23
BanI GGYRCC 1 cut(s) 766
BanII GRGCYC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 1 cut(s) 718
BccI CCATC 7 cut(s) 207, 295, 301, 404, 549, 613, 756
BciT130I CCWGG 1 cut(s) 661
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 1 cut(s) 455
BfmI CTRYAG 1 cut(s) 636
BisI GCNGC 1 cut(s) 732
BlsI GCNGC 1 cut(s) 733
Bme1390I CCNGG 2 cut(s) 83, 661
Bme18I GGWCC 1 cut(s) 602
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 3 cut(s) 85, 86, 602
BmiI GGNNCC 4 cut(s) 86, 87, 88, 768
BmrFI CCNGG 2 cut(s) 83, 661
BmsI GCATC 4 cut(s) 204, 256, 418, 448
BpmI CTGGAG 1 cut(s) 643
Bpu10I CCTNAGC 1 cut(s) 270
BpuEI CTTGAG 3 cut(s) 89, 362, 431
BpuMI CCSGG 1 cut(s) 83
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 2 cut(s) 82, 660
Bsc4I CCNNNNNNNGG 1 cut(s) 663
Bse1I ACTGG 1 cut(s) 14
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 661
BseDI CCNNGG 2 cut(s) 82, 660
BseGI GGATG 3 cut(s) 472, 560, 605
BseLI CCNNNNNNNGG 1 cut(s) 663
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 1 cut(s) 14
BseSI GKGCMC 2 cut(s) 89, 769
BseXI GCAGC 1 cut(s) 718
BshFI GGCC 4 cut(s) 23, 87, 275, 577
BshNI GGYRCC 1 cut(s) 766
BsiHKAI GWGCWC 1 cut(s) 570
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 82
BslI CCNNNNNNNGG 1 cut(s) 663
BsmAI GTCTC 1 cut(s) 61
BsmI GAATGC 2 cut(s) 445, 689
BsnI GGCC 4 cut(s) 23, 87, 275, 577
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 1 cut(s) 89
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 3 cut(s) 89, 570, 769
Bsp143I GATC 1 cut(s) 127
BspANI GGCC 4 cut(s) 23, 87, 275, 577
BspCNI CTCAG 1 cut(s) 283
BspLI GGNNCC 4 cut(s) 86, 87, 88, 768
BspMAI CTGCAG 1 cut(s) 640
BspT107I GGYRCC 1 cut(s) 766
BspTNI GGTCTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 34
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 2 cut(s) 82, 660
BssMI GATC 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 661
Bst4CI ACNGT 1 cut(s) 791
BstC8I GCNNGC 2 cut(s) 407, 717
BstDEI CTNAG 1 cut(s) 270
BstF5I GGATG 3 cut(s) 472, 560, 605
BstKTI GATC 1 cut(s) 130
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 574
BstNI CCWGG 1 cut(s) 661
BstSCI CCNGG 2 cut(s) 81, 659
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 2 cut(s) 89, 769
BstV1I GCAGC 1 cut(s) 718
BsuRI GGCC 4 cut(s) 23, 87, 275, 577
BtsCI GGATG 3 cut(s) 472, 560, 605
BtsI GCAGTG 1 cut(s) 694
BtsIMutI CAGTG 2 cut(s) 694, 787
Cac8I GCNNGC 2 cut(s) 407, 717
CaiI CAGNNNCTG 1 cut(s) 749
Cfr13I GGNCC 3 cut(s) 85, 86, 602
Csp6I GTAC 1 cut(s) 524
CviAII CATG 1 cut(s) 207
CviQI GTAC 1 cut(s) 524
DdeI CTNAG 1 cut(s) 270
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 21
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 602
Eco57I CTGAAG 1 cut(s) 570
Eco88I CYCGRG 1 cut(s) 89
EcoO109I RGGNCCY 2 cut(s) 85, 86
EcoRI GAATTC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 659
EcoT38I GRGCYC 1 cut(s) 89
FaeI CATG 1 cut(s) 210
FatI CATG 1 cut(s) 206
FauNDI CATATG 1 cut(s) 176
Fnu4HI GCNGC 1 cut(s) 732
FokI GGATG 3 cut(s) 479, 567, 592
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 732
FspBI CTAG 1 cut(s) 455
GluI GCNGC 1 cut(s) 732
GsuI CTGGAG 1 cut(s) 643
HaeIII GGCC 4 cut(s) 23, 87, 275, 577
HapII CCGG 1 cut(s) 82
Hin1II CATG 1 cut(s) 210
HincII GTYRAC 2 cut(s) 334, 472
HindII GTYRAC 2 cut(s) 334, 472
HindIII AAGCTT 1 cut(s) 483
HinfI GANTC 2 cut(s) 226, 451
HpaI GTTAAC 1 cut(s) 334
HpaII CCGG 1 cut(s) 82
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 3 cut(s) 238, 334, 472
Hpy188I TCNGA 2 cut(s) 225, 466
Hpy188III TCNNGA 3 cut(s) 379, 448, 455
Hpy8I GTNNAC 3 cut(s) 238, 334, 472
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 511, 545
HpyCH4III ACNGT 1 cut(s) 791
HpyCH4V TGCA 6 cut(s) 4, 39, 195, 638, 687, 794
HpyF10VI GCNNNNNNNGC 1 cut(s) 574
HpyF3I CTNAG 1 cut(s) 270
Hsp92II CATG 1 cut(s) 210
KspAI GTTAAC 1 cut(s) 334
Kzo9I GATC 1 cut(s) 127
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 1 cut(s) 718
LweI GCATC 4 cut(s) 204, 256, 418, 448
MaeI CTAG 1 cut(s) 455
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MboII GAAGA 7 cut(s) 288, 291, 294, 297, 300, 338, 394
MhlI GDGCHC 3 cut(s) 89, 570, 769
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 6 cut(s) 29, 391, 504, 652, 694, 802
MluNI TGGCCA 1 cut(s) 23
MnlI CCTC 5 cut(s) 163, 358, 424, 588, 667
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
MseI TTAA 5 cut(s) 114, 333, 693, 801, 805
MslI CAYNNNNRTG 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 2 cut(s) 83, 661
Mva1269I GAATGC 2 cut(s) 445, 689
MvaI CCWGG 1 cut(s) 661
MwoI GCNNNNNNNGC 1 cut(s) 574
NciI CCSGG 1 cut(s) 83
NdeI CATATG 1 cut(s) 176
NdeII GATC 1 cut(s) 127
NlaIII CATG 1 cut(s) 210
NlaIV GGNNCC 4 cut(s) 86, 87, 88, 768
PacI TTAATTAA 1 cut(s) 805
PceI AGGCCT 1 cut(s) 275
PctI GAATGC 2 cut(s) 445, 689
PfeI GAWTC 2 cut(s) 226, 451
PkrI GCNGC 1 cut(s) 733
Psp6I CCWGG 1 cut(s) 659
PspGI CCWGG 1 cut(s) 659
PspN4I GGNNCC 4 cut(s) 86, 87, 88, 768
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 3 cut(s) 85, 86, 602
PsrI GAACNNNNNNTAC 2 cut(s) 659, 691
PstI CTGCAG 1 cut(s) 640
PstNI CAGNNNCTG 1 cut(s) 749
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
RseI CAYNNNNRTG 1 cut(s) 211
SaqAI TTAA 5 cut(s) 114, 333, 693, 801, 805
SatI GCNGC 1 cut(s) 732
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 3 cut(s) 85, 86, 602
ScrFI CCNGG 2 cut(s) 83, 661
SduI GDGCHC 3 cut(s) 89, 570, 769
SetI ASST 8 cut(s) 155, 435, 487, 522, 541, 733, 775, 783
SfaNI GCATC 4 cut(s) 204, 256, 418, 448
SfcI CTRYAG 1 cut(s) 636
SinI GGWCC 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 211
SmlI CTYRAG 3 cut(s) 104, 377, 446
SmoI CTYRAG 3 cut(s) 104, 377, 446
Sse9I AATT 6 cut(s) 29, 391, 504, 652, 694, 802
SseBI AGGCCT 1 cut(s) 275
SspI AATATT 1 cut(s) 256
SspMI CTAG 1 cut(s) 455
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 2 cut(s) 81, 659
TaaI ACNGT 1 cut(s) 791
TaqI TCGA 1 cut(s) 118
TasI AATT 6 cut(s) 29, 391, 504, 652, 694, 802
TfiI GAWTC 2 cut(s) 226, 451
Tru1I TTAA 5 cut(s) 114, 333, 693, 801, 805
Tru9I TTAA 5 cut(s) 114, 333, 693, 801, 805
TscAI CASTG 2 cut(s) 694, 794
TseI GCWGC 1 cut(s) 731
TspDTI ATGAA 1 cut(s) 477
TspGWI ACGGA 2 cut(s) 502, 693
TspRI CASTG 2 cut(s) 694, 794
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 3 cut(s) 29, 391, 504
XbaI TCTAGA 1 cut(s) 454
XspI CTAG 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.