Rh4DG078700

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003467
Physical Location & Seq
Reverse (-)
11689491 .. 11695520
6030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG078700.1

Sequence Viewer

Length: 1140 bp
ATGCAGTCTTCTCCAGTAATGGCCACCAAATTCCATTGCACAAAACCCGTCGTTGTCTGTAAGCAGAGACCCAACTTTTTCCGGGGCCCCGAGAACTTCAAACTCAAGCCTTTTAATCGAAACCCGGATCATATTTTGGTCAGTCCAATCACCTCCACCATTTCTACAAGGGGCATATGGGATTTCACAAAGTGCATCAATATCTCATGGAGATGGGTTTCGCAGATTCTCTCTGTTTACTTGCTTTTTCTCAATATTGTTGCTGATGCTCAGGCCTATGATTACCCATCTCCTTCTTCTTCTTCTGTTTATGCTTGTGAAGATGTTAACAATTACTATGCCAATGTGGAGCATTTAGAGGGTAAATCACTCAAGAAGAAACTGAATTCCATCATTGGCAAGCATCAATCTTTATCTTACAGAGAGGTGTGGAATGCTCTCAAGATTCTAGATGCTTCGGATGTTGACAACCCAGAAGCTTCATCAGGGATAGTTGAAATTTACTCCGTGAAGGTTGTACCAAAGTCATTAGCTGGAAAGCCTGAAGGATGGAATAGAGAGCACTTATGGCCTCGTTCTTATGGTTTGAAAAATGGTCCATCCCTGACTGATATACACAACATTCGCCCTGCAGATGTGAATGTCAATTCCTCCAGGGGGAACAAATACTACGGAGAATGCAGTGAAAATTCGGCTAAATGTTTGAAGCCAGCAAATAAAGAAGCTGCTTTGGACACAGAAACTGACAAGGATAGATGGGCACCACCTAAACAGCATAGAGGAGATATTGCTAGGGCACTGATGTACATGGCGGTATGTTATGGATATCGTCAACCAGATGGAGGCCCAGTTCTTCACCTTTCAGATTCTCCAAAAATTTCAAAGAGTGAGATGGGACTGCTTTCTACGTTGTTAAAGTGGAATGAGATTGATCCCCCTTCAAGAGAAGAGAAATTGAGAAATGACAGAGTATGCAAGTGTTATCAACACAACAGAAATCCTTTCATTGATCATCCAGAATATGCCACTCTCATATGGAAAGAACATATCTTGAGCCATCATGCTTTCCATCCTCTTCCTAGAAGTCATCAAATTCAAAACAACAAACAAGGAAACTTTAGCAAGCAAGTCATCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

379

Amino Acids

43.23

Weight (kDa)

9.15

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 121 - 340 9e-40 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 760
AciI CCGC 1 cut(s) 812
AclWI GGATC 2 cut(s) 135, 926
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 6 cut(s) 29, 385, 498, 688, 876, 1092
AcuI CTGAAG 1 cut(s) 564
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 2 cut(s) 519, 806
AfiI CCNNNNNNNGG 2 cut(s) 657, 842
AgsI TTSAA 7 cut(s) 100, 497, 589, 706, 882, 942, 1097
AjnI CCWGG 1 cut(s) 653
AloI GAACNNNNNNTCC 2 cut(s) 834, 866
AluBI AGCT 3 cut(s) 479, 533, 725
AluI AGCT 3 cut(s) 479, 533, 725
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 2 cut(s) 135, 926
AlwNI CAGNNNCTG 1 cut(s) 743
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 5 cut(s) 21, 85, 273, 569, 844
ApaI GGGCCC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 725
ApoI RAATTY 6 cut(s) 29, 385, 498, 688, 876, 1092
AspS9I GGNCC 4 cut(s) 85, 86, 596, 845
AsuC2I CCSGG 2 cut(s) 83, 125
AsuHPI GGTGA 2 cut(s) 142, 848
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 1 cut(s) 596
BaeGI GKGCMC 3 cut(s) 89, 763, 799
BalI TGGCCA 1 cut(s) 23
BanI GGYRCC 1 cut(s) 760
BanII GRGCYC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 1 cut(s) 712
BciT130I CCWGG 1 cut(s) 655
BclI TGATCA 1 cut(s) 1009
BcnI CCSGG 2 cut(s) 83, 125
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 3 cut(s) 449, 792, 1080
BfmI CTRYAG 1 cut(s) 630
BisI GCNGC 1 cut(s) 726
BlsI GCNGC 1 cut(s) 727
Bme1390I CCNGG 3 cut(s) 83, 125, 655
Bme18I GGWCC 1 cut(s) 596
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 4 cut(s) 85, 86, 596, 845
BmiI GGNNCC 4 cut(s) 86, 87, 88, 762
BmrFI CCNGG 3 cut(s) 83, 125, 655
BmrI ACTGGG 1 cut(s) 842
BmsI GCATC 4 cut(s) 204, 256, 412, 442
BmuI ACTGGG 1 cut(s) 842
BpmI CTGGAG 1 cut(s) 637
Bpu10I CCTNAGC 1 cut(s) 270
BpuEI CTTGAG 4 cut(s) 89, 356, 425, 1072
BpuMI CCSGG 2 cut(s) 83, 125
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 2 cut(s) 82, 654
BsaXI ACNNNNNCTCC 2 cut(s) 834, 864
Bsc4I CCNNNNNNNGG 2 cut(s) 657, 842
Bse1I ACTGG 2 cut(s) 14, 848
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 655
BseDI CCNNGG 2 cut(s) 82, 654
BseGI GGATG 5 cut(s) 466, 554, 599, 1012, 1069
BseLI CCNNNNNNNGG 2 cut(s) 657, 842
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 14, 848
BseRI GAGGAG 1 cut(s) 795
BseSI GKGCMC 3 cut(s) 89, 763, 799
BseXI GCAGC 1 cut(s) 712
BshFI GGCC 5 cut(s) 23, 87, 275, 571, 846
BshNI GGYRCC 1 cut(s) 760
BsiHKAI GWGCWC 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 2 cut(s) 82, 125
BslFI GGGAC 1 cut(s) 909
BslI CCNNNNNNNGG 2 cut(s) 657, 842
BsmAI GTCTC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 909
BsmI GAATGC 2 cut(s) 439, 683
BsnI GGCC 5 cut(s) 23, 87, 275, 571, 846
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 1 cut(s) 89
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 4 cut(s) 89, 564, 763, 799
Bsp1407I TGTACA 1 cut(s) 804
Bsp143I GATC 3 cut(s) 127, 931, 1009
BspACI CCGC 1 cut(s) 812
BspANI GGCC 5 cut(s) 23, 87, 275, 571, 846
BspCNI CTCAG 1 cut(s) 283
BspLI GGNNCC 4 cut(s) 86, 87, 88, 762
BspMAI CTGCAG 1 cut(s) 634
BspPI GGATC 2 cut(s) 135, 926
BspT107I GGYRCC 1 cut(s) 760
BspTNI GGTCTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 804
BsrI ACTGG 2 cut(s) 14, 848
BssECI CCNNGG 2 cut(s) 82, 654
BssMI GATC 3 cut(s) 127, 931, 1009
Bst2UI CCWGG 1 cut(s) 655
Bst6I CTCTTC 2 cut(s) 942, 1080
BstAUI TGTACA 1 cut(s) 804
BstC8I GCNNGC 3 cut(s) 401, 711, 1124
BstDEI CTNAG 1 cut(s) 270
BstF5I GGATG 5 cut(s) 466, 554, 599, 1012, 1069
BstKTI GATC 3 cut(s) 130, 934, 1012
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 3 cut(s) 127, 931, 1009
BstMWI GCNNNNNNNGC 1 cut(s) 568
BstNI CCWGG 1 cut(s) 655
BstSCI CCNGG 3 cut(s) 81, 123, 653
BstSFI CTRYAG 1 cut(s) 630
BstSLI GKGCMC 3 cut(s) 89, 763, 799
BstV1I GCAGC 1 cut(s) 712
BsuRI GGCC 5 cut(s) 23, 87, 275, 571, 846
BtsCI GGATG 5 cut(s) 466, 554, 599, 1012, 1069
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 2 cut(s) 688, 797
Cac8I GCNNGC 3 cut(s) 401, 711, 1124
CaiI CAGNNNCTG 1 cut(s) 743
Cfr13I GGNCC 4 cut(s) 85, 86, 596, 845
Csp6I GTAC 2 cut(s) 518, 805
CviAII CATG 3 cut(s) 207, 808, 1061
CviQI GTAC 2 cut(s) 518, 805
DdeI CTNAG 1 cut(s) 270
DpnI GATC 3 cut(s) 129, 933, 1011
DpnII GATC 3 cut(s) 127, 931, 1009
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 2 cut(s) 942, 1080
EarI CTCTTC 2 cut(s) 942, 1080
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 827
Eco47I GGWCC 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 1 cut(s) 89
EcoO109I RGGNCCY 2 cut(s) 85, 86
EcoRI GAATTC 1 cut(s) 385
EcoRII CCWGG 1 cut(s) 653
EcoRV GATATC 1 cut(s) 827
EcoT38I GRGCYC 1 cut(s) 89
FaeI CATG 3 cut(s) 210, 811, 1064
FaqI GGGAC 1 cut(s) 909
FatI CATG 3 cut(s) 206, 807, 1060
FauNDI CATATG 2 cut(s) 176, 1034
FbaI TGATCA 1 cut(s) 1009
Fnu4HI GCNGC 1 cut(s) 726
FokI GGATG 5 cut(s) 473, 561, 586, 999, 1056
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 726
FspBI CTAG 3 cut(s) 449, 792, 1080
GluI GCNGC 1 cut(s) 726
GsuI CTGGAG 1 cut(s) 637
HaeIII GGCC 5 cut(s) 23, 87, 275, 571, 846
HapII CCGG 2 cut(s) 82, 125
Hin1II CATG 3 cut(s) 210, 811, 1064
HincII GTYRAC 3 cut(s) 328, 466, 833
HindII GTYRAC 3 cut(s) 328, 466, 833
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 3 cut(s) 226, 445, 866
HpaI GTTAAC 1 cut(s) 328
HpaII CCGG 2 cut(s) 82, 125
HphI GGTGA 2 cut(s) 142, 848
Hpy166II GTNNAC 4 cut(s) 238, 328, 466, 833
Hpy188I TCNGA 2 cut(s) 460, 865
Hpy188III TCNNGA 6 cut(s) 373, 442, 449, 942, 1016, 1051
Hpy8I GTNNAC 4 cut(s) 238, 328, 466, 833
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 4 cut(s) 303, 505, 539, 948
HpyCH4IV ACGT 1 cut(s) 908
HpyCH4V TGCA 6 cut(s) 4, 39, 195, 632, 681, 975
HpyF10VI GCNNNNNNNGC 1 cut(s) 568
HpyF3I CTNAG 1 cut(s) 270
HpySE526I ACGT 1 cut(s) 908
Hsp92II CATG 3 cut(s) 210, 811, 1064
Ksp22I TGATCA 1 cut(s) 1009
KspAI GTTAAC 1 cut(s) 328
Kzo9I GATC 3 cut(s) 127, 931, 1009
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 1 cut(s) 712
LweI GCATC 4 cut(s) 204, 256, 412, 442
MaeI CTAG 3 cut(s) 449, 792, 1080
MaeII ACGT 1 cut(s) 908
MalI GATC 3 cut(s) 129, 933, 1011
MboI GATC 3 cut(s) 127, 931, 1009
MboII GAAGA 8 cut(s) 288, 291, 294, 332, 388, 845, 959, 1067
MhlI GDGCHC 4 cut(s) 89, 564, 763, 799
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 9 cut(s) 29, 331, 385, 498, 646, 688, 876, 953, 1092
MluNI TGGCCA 1 cut(s) 23
MnlI CCTC 8 cut(s) 163, 352, 418, 582, 661, 773, 836, 1083
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
MseI TTAA 3 cut(s) 114, 327, 914
MslI CAYNNNNRTG 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 2 cut(s) 82, 125
MspR9I CCNGG 3 cut(s) 83, 125, 655
Mva1269I GAATGC 2 cut(s) 439, 683
MvaI CCWGG 1 cut(s) 655
MwoI GCNNNNNNNGC 1 cut(s) 568
NciI CCSGG 2 cut(s) 83, 125
NdeI CATATG 2 cut(s) 176, 1034
NdeII GATC 3 cut(s) 127, 931, 1009
NlaIII CATG 3 cut(s) 210, 811, 1064
NlaIV GGNNCC 4 cut(s) 86, 87, 88, 762
PceI AGGCCT 1 cut(s) 275
PctI GAATGC 2 cut(s) 439, 683
PfeI GAWTC 3 cut(s) 226, 445, 866
PkrI GCNGC 1 cut(s) 727
Psp6I CCWGG 1 cut(s) 653
PspGI CCWGG 1 cut(s) 653
PspN4I GGNNCC 4 cut(s) 86, 87, 88, 762
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 4 cut(s) 85, 86, 596, 845
PsrI GAACNNNNNNTAC 2 cut(s) 653, 685
PstI CTGCAG 1 cut(s) 634
PstNI CAGNNNCTG 1 cut(s) 743
RsaI GTAC 2 cut(s) 519, 806
RsaNI GTAC 2 cut(s) 518, 805
RseI CAYNNNNRTG 1 cut(s) 211
SaqAI TTAA 3 cut(s) 114, 327, 914
SatI GCNGC 1 cut(s) 726
Sau3AI GATC 3 cut(s) 127, 931, 1009
Sau96I GGNCC 4 cut(s) 85, 86, 596, 845
ScrFI CCNGG 3 cut(s) 83, 125, 655
SduI GDGCHC 4 cut(s) 89, 564, 763, 799
SfaNI GCATC 4 cut(s) 204, 256, 412, 442
SfcI CTRYAG 1 cut(s) 630
SinI GGWCC 1 cut(s) 596
SmiMI CAYNNNNRTG 1 cut(s) 211
SmlI CTYRAG 4 cut(s) 104, 371, 440, 1051
SmoI CTYRAG 4 cut(s) 104, 371, 440, 1051
Sse9I AATT 9 cut(s) 29, 331, 385, 498, 646, 688, 876, 953, 1092
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 1 cut(s) 812
SspI AATATT 1 cut(s) 256
SspMI CTAG 3 cut(s) 449, 792, 1080
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 3 cut(s) 81, 123, 653
TaiI ACGT 1 cut(s) 911
TaqI TCGA 1 cut(s) 118
TasI AATT 9 cut(s) 29, 331, 385, 498, 646, 688, 876, 953, 1092
TatI WGTACW 1 cut(s) 804
TfiI GAWTC 3 cut(s) 226, 445, 866
Tru1I TTAA 3 cut(s) 114, 327, 914
Tru9I TTAA 3 cut(s) 114, 327, 914
TscAI CASTG 2 cut(s) 688, 804
TseI GCWGC 1 cut(s) 725
TspDTI ATGAA 2 cut(s) 471, 994
TspGWI ACGGA 2 cut(s) 496, 687
TspRI CASTG 2 cut(s) 688, 804
VpaK11BI GGWCC 1 cut(s) 596
XapI RAATTY 6 cut(s) 29, 385, 498, 688, 876, 1092
XbaI TCTAGA 1 cut(s) 448
XspI CTAG 3 cut(s) 449, 792, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.